A sedimentation study of St. Ana Lake (Romania) applying the <Superscript>210</Superscript>Pb and <Superscript>137</Superscript>Cs dating methods

The biggest problem with most lakes that have no contact with other water sources and are being charged by precipitation is the massive eutrophication. The aim of this work was to determine the sedimentation rate in order to evaluate the progress of eutrophication for St. Ana Lake (Ciomad Mountain near the Băile Tuşnad in Harghita County (Romania)). The concentration of ²¹⁰Pb was determined by means of high resolution gamma spectrometry as well as derived from ²¹⁰Po activity which was measured through alpha spectrometry; values obtained are in good agreement. For the excess ²¹⁰Pb activity values between 4.0±0.5 Bq/kg and 218±20 Bq/kg have been found. As an alternative method, the ¹³⁷Cs dating method was applied as well. Calculated mass sedimentation rates are in the range of 0.06±0.01 to 0.32±0.05 g/cm² year with a mean of value of 0.15±0.02 g/cm² year. Linear sedimentation rates yielded much higher sedimentation values (between 0.5±0.1 and 7.9±0.7 cm/year with a mean of 2.4±0.6 cm/year), due to the predominant organic matter composition and the long suspension time of the sediment. This is an indication for the process of eutrophication which will probably lead to the transformation of the lake into a peat bog.     

The genetic signature of a shrunken head

The making of ritual shrunken heads, or tsantsas, was a common practice among the Jivaro-Shuar tribes of Ecuador and Peru during the post-Columbian period. The raising interest in the tsantsas in the late nineteenth through the twentieth century caused an increase in manufacturing of forged shrunken heads for profit. In the current study, we examined the authenticity and possible cultural provenance of the shrunken head displayed at the “Eretz Israel Museum, Tel Aviv” using macro- and microscopic criteria together with DNA analyses. DNA analyses have revealed that the shrunken head represents a human male individual with a genetic profile compatible with an African ancestry and resembling that of modern South American populations.     

Phytolith assaying using a micron-scale electrokinetic sorting ring

A particularly useful indicator of past vegetation are phytoliths, glassy products of plant metabolism which have distinctive size and morphology based on the plant taxa which produced them; however, their analysis is a time-consuming task. Building on investigations into mobilizing and sorting of synthetic polystyrene microspheres using a closed loop rectangular microelectromechanical systems (MEMS) electrokinetic array, we investigate these devices’ utility for sorting natural microparticles. Using phytolith samples extracted from archaeological sediment, we show that closed loop MEMS can separate the glassy bodies from both mineral contaminants and each other. Also, small differences in polarization between the phytoliths cause lateral segregation between particles of different sizes and shapes. This process facilitates manual analysis by providing a motive force to the phytoliths that translates and rotates them, showing different configurations as they are propelled by the MEMS array. The organization of the phytoliths into streams also opens the door to automated analysis using image processing. Phytolith assaying is therefore feasible using a MEMS electrokinetic ring and merits additional research to explore the potential of this innovative approach.     

Characterising the potential of sheep wool for ancient DNA analyses

The use of wool derived from sheep (Ovis aries) hair shafts is widespread in ancient and historic textiles. Given that hair can represent a valuable source of ancient DNA, wool may represent a valuable genetic archive for studies on the domestication of the sheep. However, both the quality and content of DNA in hair shafts are known to vary, and it is possible that common treatments of wool such as dyeing may negatively impact the DNA. Using quantitative real-time polymerase chain reaction (PCR), we demonstrate that in general, short fragments of both mitochondrial and single-copy nuclear DNA can be PCR-amplified from wool derived from a variety of breeds, regardless of the body location or natural pigmentation. Furthermore, although DNA can be PCR-amplified from wool dyed with one of four common plant dyes (tansy, woad, madder, weld), the use of mordants such as alum or iron leads to considerable DNA degradation. Lastly, we demonstrate that mtDNA at least can be PCR-amplified, cloned and sequenced from a range of archaeological and historic Danish, Flemmish and Greenlandic wool textile samples. In summary, our data suggest that wool offers a promising source for future ancient mitochondrial DNA studies.     

Strontium isotope analysis of human tooth enamel from Barsinia: a late antiquity site in Northern Jordan

The archaeological site of Barsinia represents a model of a mixed subsistence strategy in the late antiquity of Jordan. Contrary to historians' belief that the late antiquity economy was stagnated, archaeological evidence at the site of Barsinia points to wealth accumulation as mirrored by the local wine industry and trade. As the economic growth may enhance population dynamics, the study tests the population mobility at the site using strontium isotope ratios from the human tooth enamel. The study comprised 12 right upper third molars and 12 rodent teeth samples. The results confirm that all of the sampled individuals were local to the area (raised in the area) and whose diets were probably obtained from spatially restricted localities in the region.     

Damage caused by insects during the mummification process: an experimental study

The process of mummification had been known since the Fourth Dynasty of the Old Kingdom (ca. 2600 bc) and continued to develop throughout ancient Egyptian history. Although mummification protected the body from decay, especially by microbes, some mummification techniques left the body susceptible to insect attack. Certain types of insects have been detected in the mummies. In scholarly publications, most authors have dealt with microorganisms, while few have concerned themselves in depth with the effect of insects on the mummies. This study aims to discuss the significance of insects and the changes they affected to the mummies during embalming. To achieve this goal, experiments were carried out replicating various mummification techniques using albino Wistar rats. Analysis and investigative techniques used included visual observation, Fourier transform infrared spectroscopy, investigation of the surface morphology by a scanning electron microscope, and color change by a spectrophotometer. The following insects could be identified as being present during the second and third processes of mummification: Dermestes maculatus, Necrobia rufipes, Saprinus gilvicornis, Chrysomya albiceps, Wohlfahrtia magnifica, and Attagenus fasciatus. In addition, the majority of our findings confirmed that the degradation by insects increased with the second and third methods of mummification. Finally, the experimental study conducted using the mummification techniques of the New Kingdom (ca. 1570–1070 bc) indicated that they were more resistant to insect attack than the other types.     

Phylogeography of einkorn landraces in the Mediterranean basin and Central Europe: population structure and cultivation history

Einkorn (Triticum monococcum L.) was one of the first cereals to be domesticated in the Old World ca. 10,000 years ago and to spread towards Europe and North Africa. Its cultivation declined before the Iron Age and it remains today only as a relic crop in remote areas. To investigate if the geographic distribution of genetic diversity in modern einkorn landrace accessions could be informative about the movement of this crop during prehistory, we genotyped 50 accessions of einkorn from Europe, North Africa and the Near East. Using nuclear and chloroplast microsatellites and clustering methods, we detected two main gene pools in einkorn. The distribution of these lineages revealed differences between accessions from Morocco and the Iberian Peninsula from the rest of Europe and the Near East and suggests different regional dynamics in the spread of this crop.