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81.
Karl Heaton Caroline Solazzo Matthew J. Collins Jane Thomas-Oates Edmund T. Bergstrm 《Journal of archaeological science》2009,36(10):2145-2154
The detection and identification of protein residues from archaeological artefacts has been the subject of considerable controversy over the past two decades, this controversy spurring the application of a range of different analytical methods. Part of the debate surrounds the ability of the methods to uniquely identify original proteins. Another is the apparent contradiction between the ease of extraction in the laboratory of residues that have managed to survive in sediment over millennia; some studies use simple solvents including water, whilst others claim that efficient extraction requires the destruction of the artefact.Desorption electrospray ionisation mass spectrometry (DESI-MS) offers the potential to directly analyse proteins bound to the surface of artefacts. The method permits desorption of analytes under ambient conditions directly from a wide range of surfaces with little or no sample preparation. This has already led to its application in a range of areas, including forensic analysis of trace levels of narcotic drugs. It does not require destruction of the artefact (e.g. by drilling or dissolution) and can work in concert with other methods (for instance it could be used as a screening tool prior to lipid extraction).Here we report the construction of a DESI-MS interface and consider the potential of the method to contribute to the debate surrounding the preservation of proteins on artefacts. The DESI-MS interface was fitted to a conventional ion trap mass spectrometer and tested using peptides and proteins artificially applied to the surface of flint flakes and potsherd samples. A range of intact proteins was analysed; the DESI interface identified test proteins up to a maximum molecular weight of approximately 20 kDa. Peptides generated from these and larger proteins could be detected using DESI-MS following tryptic digestion in situ. The collected data allowed identification of the originating proteins. The potential applications to archaeological science of this new analytical tool are discussed. 相似文献
82.
83.
Paul Benneworth Lars Coenen Jerker Moodysson Björn Asheim 《European Planning Studies》2009,17(11):1645-1664
Universities are increasingly seen as potential contributors to regional innovative capacity by serving as local knowledge conduits, bringing global state-of-the-art science and technology into the region. In practice, however, more active university engagement with their regional innovation systems is not as straightforward as it may seem. The article uses examples from a successful case by which less successful regions could be inspired. Our analysis considers how various forms of technological learning intersecting within Lund University around three distinct sectoral engagement efforts have been built up and how this created new structural regional innovation capacity. 相似文献
84.
The intimate politics of secularism and the headscarf: the mall,the neighborhood,and the public square in Istanbul 总被引:1,自引:0,他引:1
The headscarf continues to be a highly charged political issue in Turkey where it is often understood through the prism of the opposition between so-called Islamists versus secularists. My work brings together feminist scholarship on the politics of everyday space and recent rethinking of the categories of secularism and religion. I begin by situating this politicized debate in the everyday material contexts of the public square, the street, and the mall. By introducing popular culture (notably the film Bü?ra) and my own fieldwork on the veil, I argue that the headscarf represents the intersection of politics of place and individual agency in a way that renders ideological debates contingent on everyday practices. Reducing the headscarf to a sign of Islamism fails to take into account the ever-shifting meanings of this object across time and space. The differences within and between the everyday urban sites I examine reveal much more complex, often contradictory, and discontinuous geographies of secularism and Islam. This analysis reveals a multiplicity that belies attempts to delineate clearly bounded spaces, subjects, and ideologies, one that is intimate and political. 相似文献
85.
Magdalena Fraser Sabine StenAnders Götherström 《Journal of archaeological science》2012,39(2):229-233
Previous research probing early migrations and contacts in the Baltic Sea area is characterized by the analysis of different chronologies and subsistent strategies on all sides of the Sea. Several studies performed on artifact typology, ceramics, grave rituals and physical anthropology ended with varying results. Although the question of human origins remains inconclusive, in this study, we rely on the phylogeography of an animal associated with humans to elucidate findings regarding prehistoric human migration and contacts.Hedgehogs, along with other fauna on Gotland, were brought over to the island by humans. We examined hedgehog mitochondrial DNA from the Pitted Ware Culture (Middle Neolithic). The genetic signatures of the animals on the island were investigated to determine the animal’s origin.From the 23 bones originally examined, twelve bones from all five locations studied yielded reliable results and resembled published extant Erinaceus europaeus sequences from Sweden, Norway and Denmark. We postulate that a western heritage for the Neolithic hedgehogs on Gotland indicates early human contact with the Swedish mainland. 相似文献
86.
This article analyses two instances of abortion law reform in Latin America. In 2006, after a decades-long impasse, the highly controversial issue of abortion came to dominate the political agenda when Colombia liberalized its abortion law and Nicaragua adopted a total ban on abortion. The article analyses the central actors in the reform processes, their strategies and the opportunity contexts. Drawing on Htun's (2003) framework, it examines why these processes concluded with opposing legislative outcomes. The authors argue for the need to understand the state as a non-unitary site of politics and policy, and for judicial processes to be seen as a key variable in facilitating gender policy reforms in Latin America. In addition, they argue that ‘windows of opportunity’ such as the timing of elections can be critically important in legislative change processes. 相似文献
87.
Abstract: The state is often described in transition: public spaces are rearranged by private companies, national social welfare is being privatized to some extent, and supranational institutions have more influence on national policies. “A view from the kitchen” (Diane Elson) is helpful for understanding the changing dynamics of states and societies because different women are affected by these policies in different and often ambiguous ways: women of the globalized South migrate to the North, creating global care chains, while women in Western industrialized countries are confronted with changing welfare regimes, leaving mainly highly educated women to profit from this situation. This article contributes to feminist debates on economic globalization and state internationalization. Our feminist materialist perspective allows a critical view on dominance and power in “governance”. Thus, the article adds to feminist debates on globalization from the perspective of state transformation and to debates on governance from the perspective of state transformation grounded in gendered social relations. 相似文献
88.
Margaret Sönser Breen 《European Legacy》2011,16(2):147-165
This essay argues that translation in Se questo è un uomo (If This is a Man) (1947), as well as in related pieces, functions for Primo Levi as a key means for claiming and potentially repairing manhood. In its capacity to reposition meaning, translation functions as a powerful vehicle for affirming agency, particularly gendered agency. What emerges in Levi's writings, particularly in Se questo's “Canto of Ulysses” chapter, is the figure of the translator as resistance fighter: the man who uses his intellect, his love of languages and other men, and his desire to communicate in order to combat the assault on humanity perpetrated by Nazism and sustained by its legacy. In this Levi's writing exists on a continuum with the cultural work of the founding members of Giustizia e Libertà and, accordingly, complicates Italy's postwar understanding of partisan activity. Throughout Se questo è un uomo and related works, translation proves a vital if imperfect means for reclaiming manhood and for asserting the possibility of friendship across cultural, regional, ethnic, and gender boundaries. 相似文献
89.
Detailed cathodoluminescence (CL) imaging of zircon crystals, coupled with Laser Ablation Multi-Collector Inductively Coupled Plasma Mass Spectrometry (LA-MC-ICP-MS) U-Pb zircon dating was used to develop new insights into the evolution of granitoids from the High Tatra Mountains. The zircon U-Pb results show two distinct age groups (350±5 Ma and 337±6 Ma) recorded from cores and rims domains, respectively. Obtained results point that the last magmatic activity in the Tatra granitoid intrusion occurred at ca. 330 Ma. The previously suggested age of 314 Ma reflects rather the hydrothermal activity and Pb-loss, coupled with post-magmatic shearing. 相似文献
90.