Ancient DNA evidence for genetic continuity in arctic dogs

The domestic dog fulfills many functions for their human companions, such as hauling, guarding, and protection. Consequently, humans have taken dogs to nearly every corner of the globe. Recent translocations of Western dogs stemming from the Victorian era dog fancy have erased some of the ancient genetic signatures of these earlier migrations. Here we used DNA of ancient and modern dogs from the North American Arctic of Alaska and Greenland to assess their genetic continuity in time and space. We successfully sequenced 23 archaeological (ca. AD 1250–1910) and recent (ca. AD 1930–1990) surface-collected Canis bone and tooth samples and compared them to 51 modern Inuit Sled Dogs and to published sequences of modern Alaskan Malamutes (and additional Inuit Sled Dogs) to test for evidence of lineage replacement or genetic continuity through time. Ancient samples from Alaska and Greenland and modern sequences from Greenland all contained a high frequency of haplotype A31, which was previously described only in modern North American Arctic dogs. Thus, A31 was a common thread tying the entire North American Arctic together prior to European colonization and, in the Eastern Arctic, indicates genetic continuity between past and present dogs as well. However, A31 is rare in modern Alaskan dogs, consistent with post-colonization replacement by Eurasian matrilines.     

The fractal geometry of ancient Maya settlement

Ancient Maya settlement patterns exhibit fractal geometry both within communities and across regions. Fractals are self-similar sets of fractional dimension. In this paper, we show how Maya settlement patterns are logically and statistically self-similar. We demonstrate how to measure the fractal dimensions (or Hausdorff–Besicovitch dimensions) of several data sets. We describe nonlinear dynamical processes, such as chaotic and self-organized critical systems, that generate fractal patterns. As an illustration, we show that the fractal dimensions calculated for some Maya settlement patterns are similar to those produced by warfare, supporting recent claims that warfare is a significant factor in Maya settlement patterning.     

New experimental data on the distance of sling projectiles

The range of stones cast by slings used in the past is debated. In the Central Andes, slings are asserted to be important weapons of prehispanic war, and have been recovered archaeologically. Rolled river cobbles and stones presumed to be slingstones found at fortified hilltop archaeological sites are presented as evidence that slings were used at these fortifications. Yet sling use has not been adequately tested at hillforts. Experiments conducted in Europe by a novice slinger have attempted to illuminate the range of sling cast stones at ancient hillforts. Data acquired from native slingers is necessary to more accurately assess distances achieved by projectiles launched by slings. We present data from sling experiments carried out in Puno, Perú among Quechua-speaking herders who are experienced slingers. The results demonstrate that a prior model of the maximum theoretical distance of sling cast stones underestimates their range. Results also show significant differences in the use of slings by men and women, and by different age groups. These new data permit a better approximation of warfare that has bearing on our interpretation of fortified sites.     

Possible isotopic fractionation of argon in source obsidians and archeological artifacts from Kulkuletti, Ethiopia

Despite uniform geochemistry ⁴⁰Ar/³⁹Ar ages of 0.6, 1.25, and 1.3 Ma on obsidians from a source flow (Worja) and from archeological artifacts at the sites of Kulkuletti (near Gademotta, Ethiopia) have been interpreted to suggest that they derive from different source flows of different ages. A further suggestion is that the magmatic source of the obsidian flows was chemically homogeneous between 1.3 Ma and 0.6 Ma. Chemical analysis of these obsidians indeed suggests that they derive from a single flow. Reexamination of the argon isotopic data reveals that argon isotopes may have been fractionated within the flow, so that the assumption of an atmospheric ⁴⁰Ar/³⁶Ar ratio of 295.5 is inappropriate to use for computing ages. Three groups of data are apparent, and internally all yield ages near 1.3 Ma if the intercept value for ⁴⁰Ar/³⁶Ar from an isotope correlation plot is used for computation instead of using the atmospheric ratio. The Soret thermal diffusion effect provides a possible mechanism of fractionation of argon within the obsidian flow that is of approximately the right magnitude to explain the observations by in-diffusion of already fractionated atmospheric argon.     

Phylogeographic analysis of barley DNA as evidence for the spread of Neolithic agriculture through Europe

Results of analyses of the photoperiod response gene (PPD-H1) and simple sequence repeats (SSRs) in modern landraces of cultivated barley were used as evidence for the mechanism of agricultural spread in Neolithic Europe. In particular, we explored the usefulness of considering adaptive genes as indicators of past selective pressures acting on crops, during their spread through Europe. In some areas, such as the Alpine region, Britain and Scandinavia, we have evidence to suggest that the adaptation of crops to certain climatic conditions may have contributed to the timing of agricultural spread. At the northern fringes of Europe, and in higher altitude locations in central Europe, the introduction of more suitably adapted cereals may have facilitated successful agriculture to trigger agricultural expansion. This research opens up the possibility of investigating other genetic adaptations to climate, which would permit a fuller evaluation of the relative contributions of climate/crop and forager/farmer interactions in the process of agricultural spread.     

Ancient DNA typing shows that a Bronze Age mummy is a composite of different skeletons

Excavations at Cladh Hallan, a Bronze Age-Iron Age settlement on South Uist in the Outer Hebrides off the west coast of Scotland, revealed the skeletons of two adults, a sub-adult and a child buried beneath the foundations of three roundhouses. Osteological and isotopic evidence has shown that the male adult skeleton is a composite made up of parts of at least three different individuals. To test the hypothesis that the female skeleton was also a composite we examined ancient DNA from four of its components: the skull, mandible, right humerus and right femur. Seven polymerase chain reactions (PCRs) were attempted, these covering positions 15,996–16,420, 16–132 and 232–368 of the mitochondrial DNA hypervariable I and II regions. Three PCRs were successful for each sample and a total of 55 sequences were obtained from the cloned products. After exclusion of possible contaminating sequences, the remaining 34 were compared. It was concluded that the mandible, humerus and femur come from different individuals. Insufficient data were obtained to draw conclusions regarding the origin of the skull. The presence of two composite skeletons at Cladh Hallan indicates that the merging of identities may have been a deliberate act, perhaps designed to amalgamate different ancestries into a single lineage.