Looking for Ancient Fish Products Through Invisible Biomolecular Residues in the Roman Production Vats from the Atlantic Coast

As organic materials, fish and its derivative products are perishable and break down rapidly and ultimately disappear. Unless they include bones or scales, the only means of identifying them in the archaeological record is through the identification of the chemical constituents that are left behind after decomposition. Fish flesh mainly consists of proteins and lipids. Since these molecules are unstable they degrade irreversibly after death or discard, losing a significant part of the original chemical elements, consequently hampering or complicating their identification. In this paper, two main types of fish products are considered: raw, salted or cooked fish, and fermented fish-based products. In the first case, the degradation of the native markers results from chemical post-depositional degradation, and eventually thermal degradation during cooking. In the second case, the biochemical processes involve bacteria that cause the rapid decomposition of fish as part of the process of forming fish sauces, such as Roman garum. To detect and identify fish products we combined experimental archaeology and organic analyses. The identification of the degradation products of cholesterol appeared to provide a strong indicator for detecting the presence of fish sauces. Analysis of samples taken from fish-salting vats located along the Atlantic coast (Marsa, Baelo Claudia, Troia, Kerlaz, and Etel) with samples taken from actual experimental garum sauces made from mackerel (Scomber scombrus), sardines (Sardina pilchardus), and/or oysters (Ostreidae), revealed the presence of common markers of fermented fish-based products. The application of the analytical methodology described here to structures involved in ancient fish sauce manufacture and storage has enabled investigation of the production of the sauces by searching directly for the fish markers on the inner sides of the containers. For the first time the addition of molluscs and fruits in the fish product has been identified as part of the process of manufacturing garum.     

Partial mummification and extraordinary context observed in perinate burials: a complex osteoarcheological study applying ICP-AES, μXRF,and macromorphological methods

Very small, green colored, partially mummified remains of a perinate individual were found buried in a ceramic pot with a copper coin in the Late Medieval cemetery of Nyárl?rinc-Hangár út (“Nyárl?rinc 3. lel?hely”) in southern Hungary. The remains must date back to the second half of the nineteenth century AD. In this paper; we present data gathered in a series of multidisciplinary investigations targeted to the partially mummified remains (ind. no. 14426) and two other non-mummified perinates (ind. no. 10662 and no. 14336) possibly buried under similar circumstances in the cemetery. Besides standard macromorphological and stereomicroscopic examinations, we compared Cu concentrations in the remains using ICP-AES and mapped Cu concentration changes using μXRF. The partially mummified perinate showed the highest Cu concentrations, while the individual buried without a possible Cu source showed the lowest. Body parts in the closer proximity of the copper coins always showed higher concentration. The mummified individual showed 497 times higher Cu values than average, and even the perinate buried without copper coin measured higher than the otherwise normal Cu content of the soil. Extremely high Cu values may be related to the corrosion of the coins included in the burials. Mummification must have been facilitated by copper deriving from the coins. Uneven Cu concentrations and only partial mummification of one of the individuals refer to the importance of other environmental factors involved in a possible quasi-natural mummification process. However, the Nyárl?rinc perinate burial no. 14426 may be the first solely copper-driven mummification case ever reported, and hopefully, more cases are to appear in the future.     

Aquatic resources in human diet in the Late Mesolithic in Northern France and Luxembourg: insights from carbon,nitrogen and sulphur isotope ratios

We investigated the contribution of freshwater resources to the diet of seven Late Mesolithic hunter-gatherers (ca. 5300–7000 BC) from Northern France and Luxembourg using stable isotope ratios. In addition to the carbon and nitrogen stable isotope ratios (δ¹³C, δ¹⁵N), we explored the potential of the sulphur isotopic ratios (δ³⁴S) to detect and quantify the proportion of protein derived from aquatic foodstuff. In only two sites, animal remains from an associated settlement were available and subsequently examined to decipher the isotopic differential between terrestrial and freshwater resources. The quantification of their relative contribution was simulated using a Bayesian mixing model. The measurements revealed a significant overlap in δ¹³C values between freshwater and terrestrial resources and a large range of δ¹⁵N values for each food category. The δ³⁴S values of the aquatic and terrestrial animals were clearly distinct at the settlement in the Seine valley, while the results on fish from Belgium demonstrated a possible overlap in δ³⁴S values between freshwater and terrestrial resources. Local freshwater ecosystem likely contributed to ca. 30–40 % of the protein in the diet of the individuals found in the Seine settlement. Out of this context, the isotopic signature and thus contribution of the available aquatic foods was difficult to assess. Another potential source of dietary protein is wild boar. Depending on the local context, collagen δ³⁴S values may contribute to better assessment of the relative contribution of freshwater and terrestrial resources.     

Identifying a technological style in the making of lime plasters at Teopancazco (Teotihuacan,México)

The aim of this article is to focus on the technology of archeological plasters at Teotihuacan (Central Mexico), from the Classic Period (200–650 A.D.), focusing in the study of Teopancazco, a neighborhood center in the city. Petrographic and chemical analyses by OM, SEM-EDS, and LA-ICP-MS were conducted to characterize samples from different constructive phases and sectors of the neighborhood center, to determine the provenance of the raw materials employed in the manufacturing (e.g., volcanic glass shards) and to assess whether a shift occurred in the manufacturing of plasters and in the procurement strategies of raw materials during the different phases. The results of the analyses show that almost no changes occurred in the making of the plaster during more than four centuries and allow us to consider the presence of a technological style in their manufacture, which is characterized by the making of plaster by mixing lime with volcanic glass shards, which in the case of Teopancazco were derived from the Altotonga (Veracruz) magmatic system. The data at our disposal suggest that this style was developed in Teopancazco and later introduced throughout the city of Teotihuacan in the Early Xolalpan phase (A.D. 350), although we still do not know whether the provenance of the raw materials is the same. The wide distribution of this technological tradition could be due to the technological properties of the materials and/or the influence of the intermediate elite who ruled over Teopancazco. The paper confirms that the study of material culture and technology sheds light on broader cultural aspects of ancient societies.     

A noble diet at the Hof van Leugenhaeghe (Steendorp,Belgium): pig skulls as a fourteenth–fifteenth century delicacy?

The animal remains found at the fourteenth–fifteenth century Hof van Leugenhaeghe are crucial to reconstruct the life of the noble inhabitants, as all buildings were destroyed with the construction of a later estate on the property called the Blauwhof. The diet confirms the high social status of this nobility with the suspected consumption of pig skulls, a possible sign of wealth in late-medieval Flanders. Other signs of a noble diet are found as well: juvenile cattle, a diverse spectrum of game, partridge and grey heron. The observed pattern of a wealthy diet is consistent with the zooarchaeological assemblages found at other noble sites in late-medieval Flanders.     

Luminescence geochronology and paleoenvironmental implications of coastal deposits of southeast Cyprus

Quaternary stratigraphy and sea level changes have been extensively investigated in many areas of the Mediterranean. However, numerical dating of coastal deposits and the associated paleoenvironmental information are limited for the coasts of Cyprus, principally based on radiometric and radiation-exposure geochronological techniques on fossils which bear a range of limitations and uncertainties. As such, optically stimulated luminescence (OSL) dating techniques are deemed to be the most suitable in direct dating of the coastal sediments of Cyprus. In the southeastern Cyprus, coastal dunes (aeolianites) now forming elongated ridges appear as morphological features running parallel to the current shoreline presenting an indicator of sea level and climate changes of great paleoenvironmental significance. We present our first chronological results for the exposed aeolianites and underlying littoral deposits formed along the southeastern coastal Cyprus ranging from 78.4 ± 9.9 to 56.2 ± 7.4 ka. The post-infrared–infrared stimulated luminescence (pIR-IRSL) revealed that dune formation took place during the marine isotope stages (MISs) 3, 4, and possibly 5a. Late Holocene reworking is proposed for a distinctively isolated dune with an age of ~1.3 ka ago. This study also showed that pIR-IRSL dating of feldspars may be a reliable alternative to quartz OSL dating when the quartz luminescence characteristics are unsuitable.     

Who ate the birds: the taphonomy of Sarakenos Cave,Greece

The taphonomic analysis of avian remains from Sarakenos Cave reveals that, contrary to previous suggestions, many bird bones excavated there represent food remains of the Eagle Owls rather than humans. The conclusion is based on the presence of traces of digestion, beak and claw punctures, and indirect evidence that includes relative preservation of particular elements, species composition, the lack of cut marks, and the absence of numerous traces of burning. Specimens with medullary bone and traces of digestion indicate that the owls killed breeding females in spring. Since it is unlikely that owls shared the cave with humans at the same time, it supports the notion based on archeological evidence that human groups did not inhabit it permanently.     

One,two, three phytoliths: assessing the minimum phytolith sum for archaeological studies

The number of phytolith studies has increased steadily in the last decades in palaeoecological as well as archaeological research, and phytolith analysis is currently recognised as a proper area of expertise within archaeobotany. This has led towards a strengthening in the standardisation of the different steps involved in analysis; e.g. sampling strategies, laboratory extraction or processing of plant material/soils for the creation of reference collections. In spite of this, counting procedures remain one of the areas that could be further developed. The aim of this paper is to assess representativeness of phytolith count size in archaeological samples and specifically to assess whether an increase in total number of individuals counted influences the number or distribution of morphotypes observed. Two statistical tests are performed to evaluate the representativeness of count size: phytolith sum variability analysis (PSVA) and morphotype accumulation curve (MAC). The analyses show the relationship among the number of counted phytoliths, the variability (that is, the number of different morphotypes identified) and the stabilisations of the MACs. Results allow us to support the standard count size in phytolith studies, which ranges from 250 to 300 particles. Together with a quick scan, this strategy should produce a precise and clear phytolith assemblage for archaeological studies.     

The measurement of taxonomic evenness in zooarchaeology

Zooarchaeologists frequently measure taxonomic evenness to document subsistence change and to understand the response of faunal communities to paleoenvironmental change. Although the measurement of evenness is commonplace, there are numerous challenges involved. Evenness indices are sensitive to changing richness, and by extension sample size, and various indices respond differently to changing taxonomic abundances (i.e., changing evenness). To refine protocol for comparing assemblages of varied sampling effort and identify indices that may be more useful for zooarchaeological applications, we examine the quantitative behavior of the widely used Shannon evenness and Simpson indices and two others more commonly used by ecologists. These indices are examined in relation to varied richness, sample size, and taxonomic abundances. We show that although zooarchaeologists are concerned with identifying and correcting for the effects of sample size on evenness, it may be appropriate to instead focus on how richness modulates evenness. Based on our analyses, we recommend the Simpson index for most zooarchaeological applications, except when comparing evenness across assemblages that are very even.     

A geometric morphometric relationship predicts stone flake shape and size variability

The archaeological record represents a window onto the complex relationship between stone artefact variance and hominin behaviour. Differences in the shapes and sizes of stone flakes—the most abundant remains of past behaviours for much of human evolutionary history—may be underpinned by variation in a range of different environmental and behavioural factors. Controlled flake production experiments have drawn inferences between flake platform preparation behaviours, which have thus far been approximated by linear measurements, and different aspects of overall stone flake variability (Dibble and Rezek J Archaeol Sci 36:1945–1954, 2009; Lin et al. Am Antiq 724–745, 2013; Magnani et al. J Archaeol Sci 46:37–49, 2014; Rezek et al. J Archaeol Sci 38:1346–1359, 2011). However, when the results are applied to archaeological assemblages, there remains a substantial amount of unexplained variability. It is unclear whether this disparity between explanatory models and archaeological data is a result of measurement error on certain key variables, whether traditional analyses are somehow a general limiting factor, or whether there are additional flake shape and size drivers that remain unaccounted for. To try and circumvent these issues, here, we describe a shape analysis approach to assessing stone flake variability including a newly developed three-dimensional geometric morphometric method (‘3DGM’). We use 3DGM to demonstrate that a relationship between platform and flake body governs flake shape and size variability. Contingently, we show that by using this 3DGM approach, we can use flake platform attributes to both (1) make fairly accurate stone flake size predictions and (2) make relatively detailed predictions of stone flake shape. Whether conscious or instinctive, an understanding of this geometric relationship would have been critical to past knappers effectively controlling the production of desired stone flakes. However, despite being able to holistically and accurately incorporate three-dimensional flake variance into our analyses, the behavioural drivers of this variance remain elusive.