Efficient but destructive: a test of the dental wash technique using secondary electron microscopy

The Boyadjian et al dental wash technique provides, in certain contexts, the only chance to analyze and quantify the use of plants by past populations and is therefore an important milestone for the reconstruction of paleodiet. With this paper we present recent investigations and results upon the influence of this method on teeth. A series of six teeth from a three thousand years old Brazilian shellmound (Jabuticabeira II) was examined before and after dental wash. The main focus was documenting the alteration of the surfaces and microstructures. The status of all teeth were documented using macrophotography, optical light microscopy, and atmospheric Secondary Electron Microscopy (aSEM) prior and after applying the dental wash technique. The comparison of pictures taken before and after dental wash showed the different degrees of variation and damage done to the teeth but, also, provided additional information about microstructures, which have not been visible before. Consequently we suggest that dental wash should only be carried out, if absolutely necessary, after dental pathology, dental morphology and microwear studies have been accomplished.     

The Conservation and Degradation of Archaeological Soil Features in Flanders and Brussels: The example of Watermaal-Bosvoorde

Ten years ago, an extensive literature study on the conservation and degradation of soil features in Flanders was conducted. The outcome was a report discussing the state of preservation. The present contribution intends to synthesize the chief outcomes of that research and to present a case study where the theoretical knowledge and recommendations then put forward have been applied.     

Urban geoarchaeology and environmental history at the Lost City of the Pyramids,Giza: synthesis and review

Sediment accretion in ancient urban sites and tells records a combination of cultural and geomorphic processes. Urban geoarchaeology is focused on site accumulation, collapse, weathering and erosion, as constrained by architectural plans and structures. These may document settlement growth and decay, as well as environmental history, posing a multidisciplinary challenge of interactive and fluctuating processes.     

Dietary analysis of Piraino 1, Sicily,Italy: the role of archaeopalynology in forensic science

Pollen from intestinal contents of mummies, backed by macrofloral analysis, provides important clues to diet, medicines, and season of death. Intestinal contents were recovered from the Piraino 1 mummy from the “Sepulcher of the Priests”, Piraino Mother Church, in the province of Messina, Sicily. Using standard palynological methods and pollen concentration technique, we quantified the numbers of pollen grains per gram of coprolite. The pollen spectrum was dominated by Polygalaceae, the Milkwort Family. Polygalaceae pollen is rarely found in archaeological sites. Based on comparison to published keys, we determined that the pollen came from a species of Polygala. Polygala contains species with medicinal value. We found nine other pollen types. Traces of Potamogeton (pondweed) was observed in preliminary scans. Multiple grains of Brassicaceae (mustard family), cereal (cultivated grains), Typha (cattail) and Cheno-am were found. Single grains of Castanea (chestnut), Fabaceae (bean family), Salix (willow), and Solanaceae (tomato family) were found. The preservation of the pollen was poor except for Polygala and the cereal pollen. Brassicaceae and Cheno-am have been part of the natural pollen spectrum in Sicily since ancient times. Cereal grains were consumed with prepared food. Importantly, background arboreal pollen was nearly absent. This indicates that Piraino 1 died during months of low pollination. The absence of olive pollen is important since this plant in Sicily reaches its maximum pollination in May and June and tapers off rapidly by mid-June. Therefore, absence of the key warm season airborne pollen type suggests a post-June death. Macrofossils, especially residue from grape pulp, indicates a death in September to November. His cause of death was likely a result of multiple myeloma. The methodological differences between archaeopalynology and forensic palynology are summarized. We suggest that the palynological methods presented here should be adopted for human remains analysis in forensic palynology.     

Environmental history in the Mediterranean world: cross-disciplinary investigation of cause-and-effect for degradation and soil erosion

Environmental history is a multidisciplinary enterprise united by shared interests in ecological change and the complex interactions between people and the environment. Its practitioners include expertise in the natural sciences, in history or archaeology, or in political ecology and related social sciences; but there is no agreement on a common agenda and limited success in bridging methodological and epistemological divisions that impede integrative and interdisciplinary research. World-systems history and environmental history also have overlapping interests in long-term change and matters of sustainability. The Mediterranean world sustained agricultural lifeways across some 8000 years, yet its environment has repeatedly been described as degraded, suggesting conceptual confusion between transformation and destruction. This paper is didactic in purpose and uses landscape histories for the Peloponnese and eastern Spain to show that the impact of recurrent, excessive precipitation events and of reduced quality of land cover are difficult to unravel, because they commonly appear to work in tandem. As a result (a) environmental change cannot be assumed or “predicted”, but must be studied inductively by experts with science skills, and (b) cause-and-effect relationships demand an understanding of ecological behavior, for which humanistic insights are indispensable. Social science models highlight systemic relationships from socioeconomic and structural perspectives, but are less suited to deal with the complexity of environmental change or the contingencies exemplified by human resilience. Near Eastern, Greek and Roman agronomic writings offer elite “voices” that speak to cumulative technological change, scientific understanding, and the context of intensification. Rural voices can be heard through ethnography, and in eastern Spain are extended into the past by archaeology and archival research. In the absence of structural constraints, they reveal collective decision-making with respect to a shifting repertoire of agricultural strategies that take into account market opportunities, demographic growth, finite resources and environmental problems. Such adaptability spells resilience, and “good farming” is culturally embedded as a civic responsibility, both in the ethnographic present and in the older, elite agronomic writings. But if the “moral economy” erodes in the wake of food stress, tax extortion, instability, insecurity, or ideological oppression, there is little incentive to pursue long-term strategies, so that behavior focuses on short-term survival. The context for this dialectic of poor versus good ecological management may be structural, but cause-and-effect in the traditional Mediterranean world ultimately depended on ecological and human resilience. Long-term sustainability is similarly non-predictive. It depends on people, rather than social theory.     

Evaluating chloroplast DNA in prehistoric Texas coprolites: medicinal,dietary, or ambient ancient DNA?

Molecular analysis of coprolites from Hinds Cave, Texas recovered chloroplast DNA sequences. The sequences were interpreted as evidence of diet. We analyzed 19 Hinds Cave coprolites to evaluate the potential sources of the chloroplast DNA (cpDNA) and compared our results to previous studies. This review shows that some cpDNA sequences could be from well-known prehistoric plants foods. Some other sequences could have come from ambient plant material in the guts of small animals eaten by humans in antiquity. Using pollen concentration analysis, we identify sources of ambient plant material which could have been inhaled or imbibed. It is even possible that cpDNA sequences are from proplastids within ambient pollen grains themselves. However, three sequence types cannot be explained as resulting from only dietary or ambient sources. We suggest instead that these might be from medicinal or hallucinogenic plants. We compared these three sequences to existing sequences in the GenBank. We found that these sequences are 100% matches for Rhamnus, Fouquieria, and Solanum.