Efficient but destructive: a test of the dental wash technique using secondary electron microscopy

The Boyadjian et al dental wash technique provides, in certain contexts, the only chance to analyze and quantify the use of plants by past populations and is therefore an important milestone for the reconstruction of paleodiet. With this paper we present recent investigations and results upon the influence of this method on teeth. A series of six teeth from a three thousand years old Brazilian shellmound (Jabuticabeira II) was examined before and after dental wash. The main focus was documenting the alteration of the surfaces and microstructures. The status of all teeth were documented using macrophotography, optical light microscopy, and atmospheric Secondary Electron Microscopy (aSEM) prior and after applying the dental wash technique. The comparison of pictures taken before and after dental wash showed the different degrees of variation and damage done to the teeth but, also, provided additional information about microstructures, which have not been visible before. Consequently we suggest that dental wash should only be carried out, if absolutely necessary, after dental pathology, dental morphology and microwear studies have been accomplished.     

Dietary analysis of Piraino 1, Sicily,Italy: the role of archaeopalynology in forensic science

Pollen from intestinal contents of mummies, backed by macrofloral analysis, provides important clues to diet, medicines, and season of death. Intestinal contents were recovered from the Piraino 1 mummy from the “Sepulcher of the Priests”, Piraino Mother Church, in the province of Messina, Sicily. Using standard palynological methods and pollen concentration technique, we quantified the numbers of pollen grains per gram of coprolite. The pollen spectrum was dominated by Polygalaceae, the Milkwort Family. Polygalaceae pollen is rarely found in archaeological sites. Based on comparison to published keys, we determined that the pollen came from a species of Polygala. Polygala contains species with medicinal value. We found nine other pollen types. Traces of Potamogeton (pondweed) was observed in preliminary scans. Multiple grains of Brassicaceae (mustard family), cereal (cultivated grains), Typha (cattail) and Cheno-am were found. Single grains of Castanea (chestnut), Fabaceae (bean family), Salix (willow), and Solanaceae (tomato family) were found. The preservation of the pollen was poor except for Polygala and the cereal pollen. Brassicaceae and Cheno-am have been part of the natural pollen spectrum in Sicily since ancient times. Cereal grains were consumed with prepared food. Importantly, background arboreal pollen was nearly absent. This indicates that Piraino 1 died during months of low pollination. The absence of olive pollen is important since this plant in Sicily reaches its maximum pollination in May and June and tapers off rapidly by mid-June. Therefore, absence of the key warm season airborne pollen type suggests a post-June death. Macrofossils, especially residue from grape pulp, indicates a death in September to November. His cause of death was likely a result of multiple myeloma. The methodological differences between archaeopalynology and forensic palynology are summarized. We suggest that the palynological methods presented here should be adopted for human remains analysis in forensic palynology.     

Biological mineral content in Iberian skeletal cremains for control of diagenetic factors employing multivariate statistics

The aim of this study was to define a strategy for a correct selection of bone samples by employing inductively coupled plasma optical emission spectroscopy (ICP-OES) for reconstructing the biological mineral content in bones through the determination of major elements, trace elements and Rare Earth Elements (REE, lanthanides) in skeletal cremains of ancient Iberians (III–II B.C), discovered in the Necropolis of Corral de Saus (Moixent, Valencia) between 1972 and 1979. The biological mineral content was determined taking into account diagenetic factors. A control method for a better reading of results was applied. To explore large geochemical datasets and to reduce the number of variables, Principal Component Analysis (PCA) was used, thus, providing a deeper insight into the structure of the variance of the dataset. PCA shows that the elemental profiles of bone and soil samples are clearly different. Bone samples obtained from the outer bone layer were shown to have a different elemental composition; more similar to soil samples than samples of the inner bone layer. PCA scores and loadings plots were preferred to dendrograms obtained using Cluster Analysis, due to the limits of the latter one to appreciate the spatial ordering of samples. Partial least squares discriminant analysis (PLS-DA), a frequently used supervised classification method, was applied to differentiate between degradation states of bone samples. PLS-DA results obtained in this study confirmed that changes derived from different burning conditions were associated with transformations in the mineral part of the bones. Accordingly, carbonized bones can be differentiated from cremated bones. Class assignment of bone samples with uncertain thermal conditions in dependence on their elemental composition has shown to be feasible. Consequently, for biochemical-archaeological studies the analysis and statistical classification of carbonized and cremated archaeological bones, as well as those exposed to unknown thermal conditions together with experiments in modern bones, are recommended.     

Prehistoric animal use on the Great Hungarian Plain: A synthesis of isotope and residue analyses from the Neolithic and Copper Age

The archaeological record of Eastern Hungary indicates that settlement patterns, subsistence strategies, ceramic style, trade patterns and mortuary customs changed from the Late Neolithic to the Copper Age (5000–2700 cal BC). Despite a rich archaeological tradition, questions remain regarding the management and use of domesticated animals and the role animal husbandry played in social change during this transition. Some researchers have hypothesized that these changes reflect a shift towards an economy that intensified its focus on primary and perhaps secondary animal products. Here we synthesize isotope data from human and animal remains and residue analysis from pottery sherds from Neolithic and Copper Age assemblages. Results indicate that the consumption and use of animal protein and fat was relatively high for both periods, with an increase in animal fats in ceramic vessels during the Middle Copper Age; however, milk products do not appear to have played an important dietary role. We conclude that livestock management remained small-scale during the Neolithic and Copper Age and that dairy use was minimal. It is proposed that the cultural changes that occurred at this time were associated with the emergence of smaller, independent farmsteads and perhaps the innovative use of secondary products like manure.     

Navigating the housing crisis: A comparison of international students and other newcomers in a mid-sized Canadian city

This article investigates the housing experiences of international students in comparison to other newcomers in the mid-sized Canadian city of St. John's, Newfoundland and Labrador, with a focus on how they navigate housing crises. Drawing on recent literature on housing justice, a quantitative survey of 188 participants, and 30 qualitative interviews, the findings reveal that international students and other newcomers are at different stages of their housing careers, have different needs and goals, and are experiencing the affordability crisis differently. Housing discrimination is a pressing concern, especially for international students who are subjected to intersectional prejudice, exploitation by landlords, and amplified challenges due to the COVID-19 pandemic. The article argues for inclusive housing and immigration policies that acknowledge international students as part of the Canadian housing market and ensure their rights to housing.