Distinguishing between archaeological sheep and goat bones using a single collagen peptide

We describe a method of isolating and analyzing a single collagen peptide able to distinguish between sheep and goat bone collagen. The 33 amino acid peptide from both sheep and goat collagen was sequenced and shown to differ between the two species at two positions. Analysis of a range of caprines indicated that the sequence changes occurred between the divergence of the Himalayan tahr (Hemitragus jemlahicus) and the ibex (Capra ibex) and that the proposed goat marker is diagnostic of all Capra species and breeds. The survival of these markers in archaeological bones was tested using a set of 26 ovicaprid specimens from Domuztepe, a Neolithic site in south central Turkey. These markers were used to test the osteological determination of 24 of the Domuztepe bones, and determine the species for two immature specimens. The collagen-peptide method has advantages over other non-morphological methods of sheep/goat distinction because of the long-term survival of collagen over other biomolecules such as ancient DNA. The results also highlighted the problems in relying upon one morphological criterion, in this case on the distal radius, to distinguish between sheep and goat bones.     

Using Enthesis Robusticity to Infer Activity in the Past: A Review

In bioarchaeological studies, entheseal change is commonly used to infer patterns of activity in past populations. This category of skeletal modification includes both pathological changes (enthesopathies) and changes in size, shape and surface complexity (robusticity). Despite the recent impetus to reassess the use of enthesopathies as markers of activity, robusticity has received little critical attention. In this review, we reassess key assumptions that underpin the use of robusticity in activity studies, drawing on anatomical, physiological, biomechanical and sports medicine literature sources. We find that, whilst there is some evidence to support the assumption that variation in enthesis robusticity reflects different activity patterns, little is known about the process through which this variation is produced. Presently, the stimuli (dynamics of muscle use or muscle size) for entheseal adaptation are ill-defined, a situation that limits our interpretive abilities. Consideration of bone functional adaptation principles and of the relationship of entheses to soft tissue also highlights how other factors, such as age, sex and genetic background, may influence enthesis robusticity and obscure activity-related adaptation. Understanding how these factors influence enthesis robusticity helps define how robusticity studies should be controlled, but further research is required to clarify how these factors interact with activity in robusticity development and the precise relationship between activity and robusticity. Ultimately, this review emphasises the complexity of entheseal structures and their morphological development. Any interpretation of activity from enthesis robusticity should be approached with caution, but in some circumstances, the endeavour may be ill-advised.     

Selling Maize in Mexico: The Persistence of Peasant Farming in an Era of Global Markets

The last decade of the twentieth century was heralded as the ‘end of agrarian reform’ in Mexico and the initiation of a new era of market‐led agricultural policy and practice. The impact of neoliberalism and the North American Free Trade Agreement on smallholder maize production has been widely conceived as negative, associated with ecological degradation, rural emigration and cultural erosion. Yet, some twenty years later, all evidence suggests that smallholder maize production is continuing in Mexico, albeit in evolving structures and forms. This article uses a farm‐level survey implemented in three Mexican states to assess the current condition of maize farming in Mexico. The authors revisit past categorizations of Mexican farmers and apply similar approaches to explore what maize‐producing households are doing with their maize, and what current patterns of production imply for future Mexican maize policy. They find evidence of greater persistence and adaptability in Mexican maize farming than is often presented. On the basis of their analysis, they advocate for a reconsideration of the underlying assumptions of public policy, highlighting the heterogeneity of the maize landscape and the unrealized and generally unrecognized potential this heterogeneity represents.     

Fish ’n chips: ZooMS peptide mass fingerprinting in a 96 well plate format to identify fish bone fragments

Fish are a large, highly diverse, and anthropologically important group of vertebrates. However, fish bones are underrepresented in the archaeological literature because they are less stable than those of other taxa and identification of bone to species is often difficult or impossible. We explore a new identification system, ZooMS (Zooarchaeology by Mass Spectrometry), which is based upon protein barcoding. As proteins can be cleaved enzymatically and analyzed by mass spectrometry in a repeatable way, protein barcoding is used widely in microbiological contexts for quick and inexpensive protein identification; mass spectra reflect the differences in protein sequence and can therefore be reproducibly linked to a particular protein or protein fragment. ZooMS uses peptide fingerprinting of bone collagen as a method for rapid identification of archaeological bone. This has involved the identification of masses related to peptides of known sequence. For mammals, sufficient sequence information is available for this approach but for groups, such the teleost fish, species are highly diverse and there are few available collagen sequences. Here we report a preliminary investigation into the identification of fish species by peptide mass fingerprinting that does not require sequence information. Collagen mass spectra are used to identify eight species of bony fishes (Osteichthyes) from four orders: Clupeiformes, Salmoniformes, Gadiformes, and Perciformes. The method is applied to both modern and archaeological fish remains and offers the capacity to identify traditionally unidentifiable fish fragments, thus increasing the Number of Identified Specimens (NISP) and providing invaluable information in specialized contexts.     

Shopping for Schools: How Do Marginal Consumers Gather Information About Schools?

The theory of the marginal consumer holds that a subset of better informed consumers can create a globally more efficient market through their purchase decisions. In the market for education created by recent school choice initiatives, these "market mavens" are essential to the successful functioning of the choice system given the empirically documented low quantity and quality of information possessed by the average consumer. Little is known, however, about the differences between how marginal consumers and average consumers of education search for information and make decisions about their children's schooling.     

"De-humanised females and Amazonians": British wartime fashion and its representation in Home chat, 1914-1918

Popular representations of the fashionably dressed female body between 1914 and 1918 were highly contradictory and, as this essay will show, were sharply delineated in Home Chat, one of a growing number of relatively new women's magazines which addressed the needs of an expanding skilled working-class and lower-middle-class female readership. Aiming to provide women with practical advice about all aspects of their daily lives, from the traditional concerns of fashion and beauty, marriage and children, to the more contentious issue of women's aspirations beyond the home, Home Chat was uniquely placed to reflect shifting gender and class relations.     

Exceptional preservation of a prehistoric human brain from Heslington,Yorkshire, UK

Archaeological work in advance of construction at a site on the edge of York, UK, yielded human remains of prehistoric to Romano-British date. Amongst these was a mandible and cranium, the intra-cranial space of which contained shrunken but macroscopically recognizable remains of a brain. Although the distinctive surface morphology of the organ is preserved, little recognizable brain histology survives. Though rare, the survival of brain tissue in otherwise skeletalised human remains from wet burial environments is not unique. A survey of the literature shows that similar brain masses have been previously reported in diverse circumstances. We argue for a greater awareness of these brain masses and for more attention to be paid to their detection and identification in order to improve the reporting rate and to allow a more comprehensive study of this rare archaeological survival.