The survival of PCR-amplifiable DNA in cow leather

We have investigated the survival of PCR-amplifiable mitochondrial and nuclear DNA in a small number of modern and medieval bovine leather samples. The results of this preliminary investigation demonstrate that, while no nuclear DNA can be PCR-amplified from any of the specimens, mitochondrial DNA can be amplified from all samples. To investigate this contrasting pattern of DNA survival further, we have vegetable-tanned cow skin in our own laboratory, and directly assayed the survival of PCR-amplifiable mitochondrial and nuclear DNA at each step of the process. The results indicate that nuclear DNA is reduced to sub-amplifiable levels as a result of the tanning baths, whereas amplifiable mitochondrial DNA survives the complete process. Our results suggest that old and archaeological bovine leather may represent a useful source of genetic information, although this information will most likely be limited to that which can be gained from mitochondrial DNA.     

Insurgents against Brussels: Euroscepticism and the right-wing populist turn of the Lega Nord since 2013

Under the leadership of Matteo Salvini, the Lega Nord has shifted away from its previous political identity as a voice for Italy’s north and has placed hostility towards the policies and institutions of the European Union (EU) at the heart of its rhetoric. Nowadays, the enemy is Rome no longer: it is Brussels, European institutions, and the threat to the national sovereignty posed by the EU. Borrowing from the Italian political philosopher Nicola Matteucci, we would describe Salvini’s Lega as a ‘populist insurgency’. That is to say, it is a populist party that marries the traditional populist evocation of the virtues of the people against the corrupt elites, with a pervasive glibness of analysis.