Wild or domesticated: DNA analysis of ancient water buffalo remains from north China

Recent zooarchaeological studies on water buffalo (Bubalus sp.) remains from China and south Asia question the traditional view that water buffalo were first domesticated in Neolithic China over 7000 years ago. The results from several recent population genetic studies of modern domesticated buffalo (Bubalus bubalis) are not consistent with each other, placing the original center of buffalo's domestication in south Asia, southeast Asia, or China. This paper reports a study using an ancient DNA approach to analyze water buffalo remains from Neolithic sites in north China to investigate their affinities with modern domesticated water buffalo, and to shed light on the origin of modern domesticated water buffalo in China.A 169 bp fragment of D-loop mitochondrial DNA was successfully amplified and verified for 13 of 24 bone samples obtained from seven archaeological sites along the Wei River valley in Shaanxi Province, China. The bone samples which yielded positive DNA can be dated to 8000–3600 cal. BP. The phylogenetic analysis of the obtained DNA sequences along with modern water buffalo sequences indicated that the ancient water buffalos were not the direct ancestor of modern domesticated water buffalo. However, the phylogenetic analysis, along with BLAST searches of these ancient DNA sequences, did demonstrate their relatedness to water buffalo more so than to any other bovid species, confirming the existence of indigenous wild (but now extinct) water buffalo species (B. mephistopheles) in ancient China.The DNA analysis of these ancient remains failed to establish direct links between modern domesticated water buffalo (B. bubalis) and indigenous water buffalo (B. mephistopheles) from ancient China. If further DNA studies of more ancient remains from other regions of China confirm the observation of solely indigenous water buffalo species in ancient China, it would suggest modern water buffalo might not have been first domesticated in China.     

Morphological taphonomic transformations of fossil bones in continental environments,and repercussions on their chemical composition

The aim of this paper is to provide a summary of structural or surface modifications of vertebrate bone remains in continental palaeoenvironments that may have repercussions on their chemical composition. Both before and after burial, a different set of physical, chemical and biological agencies may produce modifications of the bone morphological structure and/or bone chemical composition. Several of these morphological modifications are diagnostic of particular agents, which otherwise may not be noticed or identified in a fossil association. In order to understand diagenesis, those events that occurred before final burial have to be considered, as they may strongly modify the bone morphological structure and influence post–burial changes.     

Tracing the source of Late Neolithic Spondylus shell ornaments by stable isotope geochemistry and cathodoluminescence microscopy

Determination of the source of Spondylus objects is essential for the interpretation of Late Neolithic exchange systems and the social role of shell ornaments. We performed stable isotope analysis combined with cathodoluminescence microscopy study on ornaments (beads, bracelets) made of Spondylus shells excavated at the Aszód-Papi földek archaeological site in Hungary, to define their origin. For comparison Spondylus finds from Neolithic sites of Greece, modern Spondylus shells from the Aegean and the Adriatic, as well as fossil Spondylus and Ostrea shells from the Carpathian Basin were also examined. Oxygen isotope composition of Spondylus finds from Aszód ranges between ?1.9 and 2.1‰ and overlaps with the oxygen isotope range of shell objects from other Neolithic sites. Modern Spondylus shells from the Aegean and the Adriatic show overlapping δ¹⁸O values with one another and with the Neolithic objects; while recent shells of the Black Sea clearly are separate isotopically from the Mediterranean ones and most of archaeological artefacts. Spondylus shells from the Aszód site have Mediterranean origin; their source can be the Aegean or the Adriatic. Based on a former strontium isotope study the use of fossil Spondylus shells is excluded as raw material used for ornaments, however, in recent years the use of fossil shells was reintroduced. The shell ornaments from Aszód-Papi földek and the fossil oyster shells collected from the Carpathian Basin exhibit some overlapping oxygen isotope values; however, cathodoluminescence microscopy indicates that the Spondylus objects retained their original aragonite material. Diagenetic calcite, which occurs typically in the fossil shells, was not detected in the ornaments suggesting that the studied objects were made of recent shells. Calcitic parts observed in some Spondylus objects are not related to fossilisation.     

Use of robust methods to determine quadratic surfaces: application to heritage

3D surveys of archaeological elements and the construction of virtual models enable us to reconstruct historical sites. They provide useful data to select specific building processes.Photogrammetry and Terrestrial Laser Scanning (TLS) are the most common techniques to obtain synthetically heritage models. These techniques provide massive data which can only be managed by replacing them by primitive shapes, thereby obtaining more simple models and the explicit geometry of the element. Statistical and robust methods are used for this substitution, thereby avoiding the common errors due to measuring. Of these methods, the most widely used are the least-median-of-squares (LMedS) and the RANSAC method.Another computation method consists in recursive processes based on evolutive-type algorithms to adjust the model to the data captured.This paper describes two surface computation methodologies to determine the modelling of a column shaft located in temple G of the archaeological park of Selinunte (Sicily).