A NEW METHOD FOR EXTRACTION,ISOLATION AND TRANSESTERIFICATION OF FREE FATTY ACIDS FROM ARCHAEOLOGICAL POTTERY

This paper presents evidence for increased recovery of organic residues from archaeological pottery through use of a microwave‐assisted liquid chromatography protocol. C_16:0 and C_18:0 saturated fatty acids were obtained from archaeological potsherds recovered from nine Neolithic settlements in the Middle East dating between 4700 and 7300 cal bc , including materials that had not produced evidence for the survival of any lipid species through use of ‘conventional’ solvent extraction techniques. Compound‐specific isotopic analyses of C_16:0 and C_18:0 fatty acids in potsherd extracts subsequently revealed δ¹³C/δ¹²C compositions consistent with modern subcutaneous fats of wild boar and goats pastured on lands adjacent to the Jordan Valley, and residues from a modern pottery vessel used in the manufacturing of butter, cheese and yogurt in central Turkey. These results are presented as an illustration of capabilities of the microwave‐assisted recovery protocol. The reclamation of saturated and unsaturated fatty acids from archaeological pottery fragments recovered from a number of the earliest ceramic horizons in the Middle East is herewith reported, and the extraction methods and instrumental analytical techniques are described.     

EFFECTS OF MILLING: A POSSIBLE FACTOR INFLUENCING THE DURABILITY OF HISTORICAL MORTARS

In their literary texts, some classical Roman authors such as Gaius Plinius Secundus and Marcus Vitruvius Pollio stress the importance of milling when preparing mortars. Following these indications, this work describes the effects of milling between lime (calcium hydroxide) and clay brick powder before and after the addition of water. Starting and resulting materials were investigated by XRPD, SEM/EDS, differential scanning calorimetry and thermogravimetry (DSC/TC), colorimetry, densimetry and porosimetry. Prolonged milling changes the mineralogy of the starting materials and increases their reactivity. The final lime pastes, starting from materials milled for varying periods of time, had different mineralogical and physical characteristics. These results may help to clarify the reasons for the marked differences in durability of some historical mortars.     

OXYGEN AND CARBON ISOTOPE ANALYSIS OF HUMAN TOOTH ENAMEL FROM THE NEW KINGDOM SITE OF TOMBOS IN NUBIA

This study examines the feasibility of using oxygen isotope analysis of tooth carbonate to identify immigrants from the New Kingdom site of Tombos in Nubia (n = 30). In comparison with published data, the results (δ¹⁸O_ca,VSMOW = 31.4‰) imply a substantial presence of immigrants. However, further analysis of these data strongly suggests the need to consider hydrological and cultural factors such as the contribution of aquifer waters to the Nile, use of groundwater sources, and differences in handling and storage of water. The carbon isotope analysis, in conjunction with published ⁸⁷Sr/⁸⁶Sr data, provides additional support for the presence of immigrants at Tombos.     

Site maintenance practices and settlement social organization in Iron Age Karnataka, India: Inferring settlement places and landscape from surface distributions of ceramic assemblage attributes

This paper investigates the spatial organization of social relations in settlement contexts through a quantitative and distributional analysis of surface ceramic attributes from Iron Age Period (1200–300 BC) archaeological sites in Southern India. The results discern variation in depositional contexts across each site, from which I infer a variety of basic settlement activity structures (e.g., site maintenance, trash disposal, residence, animal husbandry, metallurgy, ritual). I use these results, together with further analyses of artifact and feature distributions, to infer a basic suite of places, place-making practices and some of the social relations and organizational structures that produced these historically unique Iron Age settlement landscapes.     

Crops and weeds: the role of weed functional ecology in the identification of crop husbandry methods

This paper brings together the results of five present-day studies of arable weed ecology, and applies these to the identification of past crop husbandry regimes on the basis of archaeobotanical weed assemblages. The contrasting husbandry regimes covered by the present-day studies include irrigation and dry farming, fallowing and rotation, intensive and extensive cultivation, and autumn and spring sowing. The weed floras associated with these regimes were analysed in terms of the functional ecology of the species represented. These same functional attributes, that distinguished contrasting regimes, were then measured for the weed species found accompanying crop seeds in two archaeobotanical studies, of the Islamic site of Khirbet Faris, S. Jordan and Neolithic sites in central Europe. Analyses using these functional measurements resulted in the identification of irrigation at the former and in the identification of sowing time and intensive cultivation at the latter.     

A bioarchaeological study of a Western Basin tradition cemetery on the Detroit River

Archaeological monitoring of construction in a Windsor city park on the Detroit River led to the discovery of an isolated cemetery containing the remains of eight individuals assigned to the Late Woodland Western Basin Tradition. At the request and consent of the contemporary First Nation community, tissue samples from five individuals were subjected to radiocarbon dating, mtDNA, and stable isotope analysis to confirm cultural affiliation and further understand the subsistence practices of these people. Radiocarbon dating placed the cemetery at the transition from the Younge phase (AD 900–1200) to the Springwells phase (AD 1200–1400). The stable carbon and nitrogen isotope results provide an unexpected but fuller understanding of Late Woodland Western Basin Tradition subsistence. All individuals were as enriched in carbon as those found on Iroquoian horticulturalist sites to the east, suggesting a very high reliance on maize. Nitrogen isotope values indicate that the protein component of the diet was comprised largely of high trophic level food sources, likely fish. An in situ osteological analysis identified a high number of carious lesions in the visible teeth, also suggesting a diet high in carbohydrates. The mtDNA findings support the antiquity of the Western Basin presence in Northeast North America through genetic links with the Hind Site, an Archaic site in southern Ontario. These results underscore the importance of such studies for providing novel insight into the archaeological histories and lifeways of this distinct Late Woodland tradition. This study also emphazises the need to work with descendant communities to provide them with information on the past that reflects their distinct heritage in the lower Great Lakes region.     

Radiocarbon evidence indicates that migrants introduced farming to Britain

Archaeologists disagree about how farming began in Britain. Some argue it was a result of indigenous groups adopting domesticates and cultigens via trade and exchange. Others contend it was the consequence of a migration of farmers from mainland Europe. To shed light on this debate, we used radiocarbon dates to estimate changes in population density between 8000 and 4000 cal BP. We found evidence for a marked and rapid increase in population density coincident with the appearance of cultigens around 6000 cal BP. We also found evidence that this increase occurred first in southern England and shortly afterwards in central Scotland. These findings are best explained by groups of farmers from the Continent independently colonizing England and Scotland, and therefore strongly support the migrant farmers hypothesis.     

Assessing the effects of conservation treatments on short sequences of DNA in vitro

Little is known about what effects conservation treatments used to preserve human and animal hard and soft tissues have on DNA preservation. We have developed a method to assess quantitatively the extent of lesions or strand breakage caused by conservation treatments on short sequences of DNA in vitro. The method developed enables the determination of the percentage of DNA preserved following exposure to a conservation treatment solution relative to control samples, thereby allowing the direct comparison of treatments based upon their preserving/damaging effects on a DNA sequence. Forty-three chemicals commonly used in the preparation and/or conservation of human and/or animal remains were examined. We found that the majority were damaging, in particular and as expected, acidic treatments and treatments carried out at elevated temperatures. A few, primarily organic solvents, were not damaging. The approach we have adopted can be applied to screen other treatments either used in the past or for future conservation applications as they are developed to assess their effects on DNA. How these results should be interpreted in terms of conservation and sampling is also discussed.     

Stable nitrogen isotopic examination of Norse sites in the Western settlement of Greenland

To continue to explore the impact of past human activity on the stable nitrogen isotope ratios of modern vegetation, we have measured the δ¹⁵N values of plants now growing on several ancient farmsteads in the Western Settlement of Norse Greenland. Although this new test area is some 400 km north and in a different ecoregion from the previous study area, the same isotopic effect was observed. A clear isotopic signature was found on the ancient farms which could be directly correlated to past activity areas; in particular, the infields. Further, the magnitude of the δ¹⁵N values was found to be similar between study areas, indicating the same interpretative framework can be applied in widely separate geographic regions. A first test of different plant species showed that although the magnitude differed greatly the same basic information was found for functionally different plants. These observations provide additional information to aid in future archaeological application of this newly observed phenomenon.