The influence of temperature on rehydroxylation [RHX] kinetics in archaeological pottery

Almost all archaeological ceramics undergo slow, progressive rehydroxylation by chemical combination with environmental water. The reaction is accompanied by an expansion, and also by the small but measurable mass gain that provides the basis of the RHX dating method. The rate of the RHX reaction increases with increasing temperature. Here we describe comprehensively the effects of temperature on the RHX process in relation to the dating methodology. We deal in turn with the kinetic model of the RHX reaction, the temperature dependence of the RHX rate, and the influence of varying environmental temperature on the RHX mass gain. We define an effective lifetime temperature and show how this is calculated from an estimated lifetime temperature history. Historical meteorological temperature data are used to estimate the lifetime temperature history, and this can be adjusted for long-term climate variation. We show also how to allow for the effects of burial in archaeological sites on the temperature history. Finally we describe how the uncertainties in estimates of RHX age depend on the estimates of temperature history and effective lifetime temperature.     

DNA analysis in charred grains of naked wheat from several archaeological sites in Spain

In the present work we attempt to recover endogenous ancient DNA from cereal grains preserved under different conditions: charred, partially charred and waterlogged. A total of 126 grains from naked wheat and 18 from barley from different sites on the Eastern Iberian Peninsula ranging from the beginning of agriculture in the region to the turn of the Common Era, were studied. Two different extraction protocols were used, a standard phenol–chloroform method and a silica-based DNA extraction procedure implemented for artificially charred seeds. Amplifications were directed to three markers: the large subunit of ribulose 1,5 biphosphate carboxylase (rbcL) and the microsatellite WCT12 in the chloroplast genome and the x and y subunits of the high molecular weight glutenin gene (Glu-1) in the nucleus. The first two were used to assess the preservation status of the samples, while with the third we tried to identify the wheat grains at species level. It was possible to obtain eleven positive amplifications in 8 partially charred seeds but only two amplifications of the Glu-1 gene from a single sample of the Early Bronze age were genome-specific. Different contamination sources were identified and reported. Cloning and alignment of sequenced clones showed a correspondence of the amplified fragment to modern wheat D genome haplotypes. This result suggests that the sample corresponds to hexaploid wheat (Triticum aestivum L.), thus being the first ancient DNA evidence to date for the cultivation of hexaploid wheat in the prehistoric agriculture of the Iberian Peninsula. Moreover, obtained results highlight contamination problems associated to the study of ancient archaeobotanical charred seeds suggest that the combination of a silica-based extraction method together with the amplification of specific targets is a good strategy for recovering endogenous ancient DNA from this kind of material.     

Palynological study of a Mi'kmaw shell midden,Northeast Nova Scotia,Canada

Before now, no palynological studies were made on shell midden deposits in Nova Scotia, despite a long history of archaeological research. A test study of samples taken from the shell midden at Maligomish (Indian Island) on the Northumberland Strait was conducted to determine if sufficient pollen, fern and moss spores, and other non-pollen palynomorphs (NPP's, including fungal remains, algal spores) could be recovered to allow interpretation of environmental conditions at the time of deposition. Several pollen processing methods were tested: sieving and swirling, chemical treatment with cold HCl, 5% KOH and brief exposure to 5% NH₄OH, and treatment with acetolysis solution. We recovered variable amounts of pollen, spores and NPP's from samples taken at 3–5 cm, 5–15 cm and 15–25 cm below surface. Abundant microcharcoal fragments prevented effective use of the swirling technique, but sufficient palynomorphs were recovered by chemical treatments to allow assessment of down-section changes in assemblages. Three radiocarbon ages from Mya arenaria and Crassostrea sp., and measurements of sediment grain size, moisture, organic and inorganic carbon, provide a framework for analysing palynological assemblage changes and context for interpreting differential palynomorph preservation. Despite some oxidation and microbial degradation of palynomorphs in the time interval (ca. 380–1600 cal yr BP), the midden assemblages correlate with pollen zones from marine and lake core sites within ca. 50 km of Maligomish. Shifts in abundances of algal spores, testate amoebae and aquatic plant pollen also indicate changes in depositional conditions during the time of midden use. The study provides the first reference for archaeologists attempting to use palynology for analysis of samples from Nova Scotian shell-bearing contexts.     

The effect of charring and burial on the biochemical composition of cereal grains: investigating the integrity of archaeological plant material

Stable isotope analysis of charred archaeobotanical cereal grains has the potential to provide direct evidence of crop growing conditions in the past and to refine palaeodietary predictions. If isotope values of archaeobotanical material are to be considered robust, it is necessary to characterise the compositional changes associated with their charring and burial. This study used a suite of analytical techniques, including FT-IR and solid state ¹³C NMR, to characterise changes in the biochemical composition of modern einkorn grains with heating at 230 °C for 2 h, 4 h, 8 h and 24 h, encompassing conditions that replicate their undistorted ancient counterparts. The biochemical composition of archaeobotanical charred einkorn grains was also investigated by FT-IR and solid state ¹³C NMR in order to assess the changes in composition which occur during burial. Results of FT-IR and solid-state ¹³C NMR show that heating of modern einkorn grains resulted in Maillard reactions between cereal proteins and starch, forming high molecular weight melanoidins, which contain both alkyl and aromatic carbon. Loss of low molecular weight carbon and nitrogen-containing volatiles resulted in a slight but non-systematic increase in the δ¹³C values and a systematic increase of 0.8‰ in the δ¹⁵N values of the charred einkorn grains. Solid-state ¹³C NMR shows that the ancient charred einkorn grains consisted entirely of aromatic carbon and retained a similar proportion of nitrogen to their modern 24 h charred counterparts, despite a significantly lower concentration of amino acids. This indicates that the amino acid nitrogen in the ancient charred grains was retained in the stable melanoidins whose polymeric structure makes them resistant to subsequent degradation.     

3400 years of agricultural engineering in Mesoamerica: lama-bordos of the Mixteca Alta,Oaxaca, Mexico

The origins of early Mesoamerican agricultural techniques are not well established. Our charcoal-derived radiocarbon chronology dates cross-valley check dams, or lama-bordos, buried by up to 11.5 m of sediment in arroyos near Coixtlahuaca, Oaxaca, Mexico. Now it is clear that people in highland Mexico built lama-bordos at least 3400–3500 years ago, several hundred years earlier than previously dated engineering systems in Mesoamerica. Lama-bordo systems evolved as intensively and extensively managed landscapes coeval with climate shifting to more arid conditions. They provide clear examples of human-produced stratigraphy and artificial landscapes (stair-stepped valleys). More importantly, these lama-bordo systems signal a major cultural tipping point toward sedentary agricultural life and solidify our understanding of the Neolithic transition in Mesoamerica.     

Uranium series dating reveals a long sequence of rock art at Altamira Cave (Santillana del Mar,Cantabria)

The rock art in Altamira Cave was the first ensemble of Palaeolithic parietal art to be identified scientifically (Sautuola, 1880). Due to the great thematic, technical and stylistic variety of the art in the cave, which constitutes one of the most complete Palaeolithic art ensembles, Altamira was listed as World Heritage by UNESCO in 1985. Uranium-series dating has recently been applied to figures on the decorated ceiling in the cave. Several motifs are partly covered by thin layers of calcite precipitates, whose formation process is datable by this method. The results provide the date when the calcite formed, which gives a minimum age for the underlying depictions. These results confirm that the parietal art at Altamira was produced during a prolonged period of time, at least 20,000 years (between 35,000 and 15,200 years ago), and that part of the ensemble corresponds to the Aurignacian period.     

Genetic sexing to determine the optimal discriminant functions for the analysis of archaeological remains from El Hierro (Canary Islands)

A correct sex assignment of a given bone or bone fragment is of paramount importance for the archaeologist, anthropologist and in forensic medicine. Discriminant functions, combining several anthropometric measurements obtained from individuals with known sex are useful tools for this purpose, but it is essential to know exactly the sex from which the measures are obtained. This is an easy task in modern populations, but it is problematic in ancient ones, since even when the entire skeleton is available, diagnosis of sex is not 100% accurate. Sexing by genetic methods by amplifying the first intron of the amelogenin gene constitutes a much more accurate method for sexing bones and may be the gold standard for further elaboration of discriminant functions which may serve for sexing new bones dug up in future excavations. With this aim we have genetically sexed 52 (out of 59) tibiae belonging to the prehispanic population of El Hierro, in the Canary Islands, identifying 18 women and 34 men, and then, performed discriminant functions combining several anthropometric variables. These functions show a high accuracy in sex diagnosis (94.2%; area under ROC curve = 0.954 with the best of the functions), so that they allow correct sexing of tibiae or tibiae fragments (only proximal third, distal third or midshaft). Thus, genetic sexing obviates the problem of finding an accurate gold standard for the elaboration of discriminant functions for ancient bones. This method could be applied to other populations of different antiquity and different ethnicity.     

HISTORY AND THE MANIFEST IMAGE: HAYDEN WHITE AS A PHILOSOPHER OF HISTORY1

To claim that Hayden White has yet to be read seriously as a philosopher of history might seem false on the face of it. But do tropes and the rest provide any epistemic rationale for differing representations of historical events found in histories? As an explanation of White's influence on philosophy of history, such a proffered emphasis only generates a puzzle with regard to taking White seriously, and not an answer to the question of why his efforts should be worthy of any philosophical attention at all. For what makes his emphasis on narrative structure and its associated tropes of philosophical relevance? What, it may well be asked, did (or could) any theory that draws its categories from a stock provided by literary criticism contribute to explicating problems with regard to the warranting of claims about knowledge, explanation, or causation that represent those concerns that philosophy typically brings to this field? Robert Doran's anthologizing of previously uncollected pieces, ranging as they do over a literal half‐century of White's published work, offers an opportunity to identify explicitly those philosophical themes and arguments that regularly and prominently feature there. Moreover, White's essays in this volume demonstrate a credible knowledge of and interest in mainstream analytic philosophers of his era and also reveal White as deeply influenced by or well acquainted with other important philosophers of history. White thus invites a reading of his work as philosophy, and this volume presents the opportunity for accepting it as such.