Persistence and decay of the intestinal microbiota's DNA in glacier mummies from the Alps

The study of the DNA of ancient microorganisms in human remains represents one of the newest and most promising branches of molecular archaeoanthropology. Despite the growing number of papers addressing this subject, however, the analysis of ancient bacterial DNA is still a contentious issue. The indigenous microbiota of the gastrointestinal tract of man represents a community characterized by relative constancy in species composition and proportion. As a model system, we studied the preservation of the intestinal microbiota DNA in two naturally freeze-dried human mummies found on the Alps. This kind of mummy is an ideal subject for ancient DNA investigations. The first is a male body historically dated 1918 A.D. while the second is the famous Tyrolean Iceman (3.350–3.100 B.C.). The screening of bacterial 16S rRNA gene libraries from colon samples of the two mummies (49 clones for the 1918 mummy, 119 clones for the Iceman) showed that the characteristic composition of the intestinal microbiota of man (Alpha-, Beta-, Gammaproteobacteria, Bacteroides, Clostridia) is still kept in the library from the recent mummy while the Iceman's library is almost entirely composed by the DNA of clostridia. Comparison of the intestinal data with those from the literature describing the screening of 16S rRNA gene libraries from other parts of the Iceman's body and from permafrost specimens indicates that the changes in library composition may partly be attributed to the proliferation of clostridia inside the corpses, as described in forensic literature, and partly to the differential persistence of the DNA of gram-negative bacteria and endospore-former low-GC gram-positive bacteria. The present results contribute to the issue of the authentication of claims of pathogen DNA identification in archaeological human remains.     

Foot amputation by the Moche of ancient Peru: osteological evidence and archaeological context

Three probable cases of foot amputation, with healing, in skeletal remains associated with the Moche culture (AD 100–750) of northern coastal Peru are described. Each case exhibits non‐functional tibio‐talar joints with proliferative bone occupying the normal joint space. The robusticity of the tibiae and fibulae suggest renewed weight‐bearing and mobility following recovery. The osteological evidence is consistent with details shown in Moche ceramic depictions of footless individuals. A footless Moche skeleton with wooden prostheses, described in 1913 by Peruvian physician Vélez López, appears to represent a fourth example of this procedure. The Moche surgical approach was similar to a technique that would be pioneered in western medicine by the Scottish surgeon Sir James Syme some 1500 years later. Copyright © 2000 John Wiley & Sons, Ltd.     

The church and Catholicism in contemporary Italy

Abstract

The constitutions of modern States may be seen somehow as a form of civil religion that is to be revered and that binds all people of a nation both ethically and legally. Reflecting on the constitutional architecture of a State means going deeper into the structure of societies, laws and mentalities. One of the foremost experts of the history of the Italian constitution and himself an interpreter of the fundamental Law of Italy, in this lecture Judge Sabino Cassese discusses the forms of constitutionalism adopted in Italy from the nineteenth-century Statuto Albertino down to contemporary debate on the ‘updating’ of the Italian constitution.     

Marketing Destinations with Prolonged Negative Images: Towards a Theoretical Model

Abstract

While there is a great deal of research on destination branding in general, the study of tourism marketing that deals specifically with destinations suffering from prolonged image problems is less well developed. We integrated theories from crisis communication, image restoration and destination marketing to examine media strategies used by such destinations to attract tourism, and then classified them. We utilized qualitative content analysis of tourism campaigns for destinations – primarily in problematic areas such as the Middle East, Africa, Eastern Europe – that appeared in international tourism magazines, destinations’ websites and video-sharing websites. We classified two approaches of media strategies: the ‘cosmetic approach’, relating to strategies that focus mainly on altering the destination's image and the ‘strategic approach’, relating to strategies that take comprehensive action, changing both the negative image and the problems causing it.     

Vernunft und Sprache — oder die Schwierigkeit,Kant auf Italienisch sprechen zu lassen

We can reach the exactness and univocity the scientific thougth aims at only by mastering the polysemy historically present in language. This appears even more difficult when we move from one polysemic system to another, as in translations into other languages. Thus, in Italy in the early XIXth century, the first attempts to translate Immanuel Kant's Critique of pure reason met great difficulties in understanding his new terms and led to misunderstanding and therefore rejecting his new philosophy.     

Geochemical signatures of Roman amphorae produced in the Sado River estuary,Lusitania (Western Portugal)

Several archaeological sites located in the Sado estuary, Lusitania province (Western Portugal), show evidence of an important amphorae production, mainly related to the Roman Tróia industrial centre of fish preserves. The chemical analysis of amphorae sherds found in Barrosinha, Bugio, Zambujalinho, Xarrouqueira and Vale da Cepa (Lower Sado) was performed by instrumental neutron activation analysis, and the mineralogical composition obtained by X-ray diffraction. The chemical results were compared with amphorae from the Herdade do Pinheiro production centre, also located in the Sado estuary. Application of several exploratory and discriminant techniques of multivariate statistical analysis using chemical data of Lower Sado amphorae allowed the characterization and differentiation of two reference groups, named Sado 1 and Sado 2. The geochemical patterns and the mineralogical composition of these groups indicate the use of two different raw materials, the selection of which appears to have been based on the proximity factor of the kilns. The amphorae produced in the Sado basin were differentiated from amphorae from the Gualdalquivir estuary (Villa Nueva, Cádiz, Spain) in Baetica province.