An improved PCR method for endogenous DNA retrieval in contaminated Neandertal samples based on the use of blocking primers

Neandertal skeletal remains are usually contaminated with modern human DNA derived from handling and washing of the specimens during excavation. Despite the fact that the distinct Neandertal haplotypes allow the design of specific primer pairs, for instance in most of the mitochondrial DNA (mtDNA) hypervariable region 1 (HVR1), the human contaminants can often outnumber the endogenous DNA, thus preventing a successful retrieval of Neandertal sequences. We have developed a novel PCR method, based on the use of blocking primers that preferentially bind to modern human contaminant DNA and block their amplification, and greatly improve the efficiency of Neandertal DNA retrieval. We tested the method in four El Sidrón Neandertal samples (two teeth and two bone fragments) with different contamination levels and taphonomic conditions, and we have been able to significantly increase the Neandertal yield from figures around 25.23% (5–69.6%) up to 90.18% (75.3–100%).     

Faecal biomarker and archaeobotanical analyses of sediments from a public latrine shed new light on ruralisation in Sagalassos, Turkey

A public latrine in the ‘Imperial Baths’ of Sagalassos was transformed into a dump site in the early Byzantine period. Several layers of urban waste, including ceramics, bones, glass and perhaps excrements were deposited on the floor. Faecal biomarker analyses and archaeobotanical analyses were conducted to reconstruct the history of the room. 5β-stanols of human origin, such as coprostanol, were found in the sewage channels together with mineralised plant remains, indicating a human faecal context. The botanical remains are furthermore representative of the Roman diet of the Sagalassians. Soil layers, deposited on top of the latrine floor and dating to the early Byzantine period, contained herbivore derived 5β-stanols, such as 5β-stigmastanol and epi-5β-stigmastanol. Additionally, a clear predominance of epi-5β-stanols over 5β-stanols showed that the animal dung has been subject to composting. In this period, the former latrine was clearly used as a manure production site which is further confirmed by stratigraphic evidence of large amounts of urban waste artefacts, which were commonly collected together with manure before application on the fields. The results of the present study support the theory that off-site potsherd scattering can be used as a proxy for manuring events. Additionally, the data show key evidence for vertical migration of 5β-stanols and presumably also for the leaching of bile acids.     

The Aterian from the Jebel Gharbi (Libya): New Technological Perspectives from North Africa

The purpose of this work is to examine the Aterian complex in the context of modern human origins through the analysis of the lithic industries from a geographically strategic area, the Jebel Gharbi (Libya). From a chronological point of view, the Aterian fits the time period of the Out of Africa 2 dispersal. During geoarchaeological surveys of the Jebel Gharbi carried out by the Italian–Libyan Archaeological Mission, 25 Aterian sites were found. The technology of the Aterian lithic industries from the Jebel Gharbi shows affinities both with the Aterian industries from Morocco and coeval industries from Egypt such as Taramsa. The affinities between contemporary Libyan Aterian industries, Egyptian industries, and some series from the Levant open new perspectives on the possible models of contact and displacement of human groups in a key period for the history of humankind.     

A history of failed innovation: continuous cooking and the Soviet pulp industry, 1940s–1960s

Abstract

In the years before and after the Second World War, chemical and related industries in a variety of countries experienced a surge in innovation and development. As a result, the pulp industry became a space for considerable innovation. In Sweden, Johan Richter developed the Kamyr digester, a pulp cooker that could run continuously and was adopted by industry within a decade. Prior to Richter, Soviet engineer Leonid Zherebov designed a similar cooker. But after 25 years of experiments, Zherebov’s design failed, and Soviet factories began to produce pulp using imported Kamyr digesters. This article examines the history of continuous pulp cooking in the Soviet Union as a means to understand the nature of Russian technological innovation and its failures. The paper contends that his effort failed because of the technological system developed by Soviet forestry – a system characterized by a lack of open communication among its main institutional actors and a scarcity of resources to facilitate innovation.     

Ammonite dating of latest Cretaceous mosasaurid reptiles (Squamata,Mosasauroidea) from Jordan—preliminary observations

Jagt, J.W.M., Jagt-Yazykova, E.A., Kaddumi, H.F. & Lindgren, J., April 2017. Ammonite dating of latest Cretaceous mosasaurid reptiles (Squamata, Mosasauroidea) from Jordan—preliminary observations. Alcheringa 42, 587-596. ISSN 0311-5518

Newly collected ammonoid material from the uppermost Cretaceous portion of the Muwaqqar Chalk Marl Formation exposed some 30 km southeast of the Qasr Al’Harrana area (east-central Jordan) includes medium-sized baculitids (Baculites ovatus auctorum, non Say), the sphenodiscid Libycoceras acutodorsatus (Noetling) and the pachydiscids Menuites fresvillensis (Seunes) and Pachydiscus (Pachydiscus) dossantosi (Maury). Of the two last named taxa, the former is a good marker species for the upper Maastrichtian, with records from Europe, central Chile, South India, Baluchistan (Pakistan), Australia, Madagascar and South Africa. The latter is known from the United Arab Emirates/Oman border area, from strata of (late) early to early late Maastrichtian age, as well as from more poorly constrained Maastrichtian levels in Brazil and Nigeria. A comparison with ammonoid assemblages from the Maastrichtian type area (southeast Netherlands/northeast Belgium) suggests correlation of the Muwaqqar Chalk Marl Formation with the middle/upper Maastricht Formation (Emael and Nekum members, ca 66.5–66.1 Ma) and the upper part of the coeval Kunrade Formation. However, associated ‘tegulated’ inoceramids of the Tenuipteria argentea group from the Muwaqqar Chalk Marl Formation favour equivalence with a higher level of the Maastrichtian type area, i.e., the Meerssen Member. From the upper Maastricht Formation and the equivalent upper part of the Kunrade Formation, the following mosasaur genera are currently known: Mosasaurus, Prognathodon, Plioplatecarpus and Carinodens. Interestingly, coeval strata of the Muwaqqar Chalk Marl Formation in east-central Jordan have yielded remains of a largely comparable suite comprising Prognathodon, Mosasaurus, Carinodens and an unnamed, highly derived plioplatecarpine.

John W.M. Jagt* [john.jagt@maastricht.nl], Natuurhistorisch Museum Maastricht, de Bosquetplein 6–7, 6211 kJ Maastricht, the Netherlands; Elena A. Jagt-Yazykova [eyazykova@uni.opole.pl], Opole University, Department of Biosystematics, Oleska 22, 45-052 Opole, Poland; Hani F. Kaddumi [blossom_trail@yahoo.com], Eternal River Museum of Natural History, Maroof Al’Rusafi Street, PO Box 11395, Amman 11123, Jordan; Johan Lindgren [johan.lindgren@geol.lu.se], Lund University, Department of Geology, Sölvegatan 12, 223 62 Lund, Sweden.