Late third‐millennium elite burials in Bahrain

Studies of old aerial photographs of the Bahrain burial mound fields have revealed that a small number of both Early Type (c. 2200–2050 BC) and Late Type (c. 2050–1750 BC) mounds are encircled by an outer ring wall, apparently marking out the mound as belonging to an elite. Four of these mounds have been excavated, and the results are presented. The geological differences between the Early Type and the Late Type mound landscapes are discussed.     

Molecular markers for the discrimination of Triticum turgidum L. subsp. dicoccum (Schrank ex Schübl.) Thell. and Triticum timopheevii (Zhuk.) Zhuk. subsp. timopheevii

The persistent uncertainty on the classification of the “new” glume wheat found in Neolithic and Bronze Age sites from Greece and other European settlements might be resolved only through analysis of its ancient DNA. Tools able to discriminate among different Triticum species on the basis of scarce, very damaged DNA, are therefore essential. While current attempts concentrate on DNA fragments sequencing and comparison, in some instances PCR-based selective amplification techniques might offer a cheaper and quicker alternative. The purpose of this research was therefore the identification of species-specific primers, able to distinguish caryopses of Triticum timopheevii subsp. timopheevii from those of Triticum turgidum subsp. dicoccum. Primers and their working conditions were defined and optimized using DNA from modern accessions. The ribosomal primers ITS1 tim and ITS2 tim, and the nuclear primer acetyl-coenzyme A tim clearly discriminated the sequences of Triticum timopheevii from other species. Finally, Neolithic charred wheat grains found in the sites of Sammardenchia (Pozzuolo del Friuli, Udine) and La Marmotta (Lago di Bracciano, Roma), belonging to the “new” wheat type or to emmer, were tested with the three selected primers. However, the results were not conclusive, because the samples analysed were apparently too degraded to yield useful DNA.     

Ultrathin sectioning techniques

Epoxy mixtures incorporating Araldite available in Australia have been used to embed Recent organisms, fossils and sedimentary rocks. Resultant sections of embedded material may be less than 10 micrometres thick and give increased resolution of fine structures. Mixtures used and sectioning techniques are described.