Interpreting the expansion of sea fishing in medieval Europe using stable isotope analysis of archaeological cod bones

Archaeological fish bones reveal increases in marine fish utilisation in Northern and Western Europe beginning in the 10th and 11th centuries AD. We use stable isotope signatures from 300 archaeological cod (Gadus morhua) bones to determine whether this sea fishing revolution resulted from increased local fishing or the introduction of preserved fish transported from distant waters such as Arctic Norway, Iceland and/or the Northern Isles of Scotland (Orkney and Shetland). Results from 12 settlements in England and Flanders (Belgium) indicate that catches were initially local. Between the 9th and 12th centuries most bones represented fish from the southern North Sea. Conversely, by the 13th to 14th centuries demand was increasingly met through long distance transport – signalling the onset of the globalisation of commercial fisheries and suggesting that cities such as London quickly outgrew the capacity of local fish supplies.     

The origin of black pigmentation in a sample of Mexican prehispanic human bones

The study of black patches and spots in prehispanic bones of Tlatelolco and Tlapacoya is presented. The chosen characterization techniques, X-ray Fluorescence, X-ray Diffraction, Scanning Electron Microscopy, Energy Dispersive X-ray Spectroscopy, Infrared Spectroscopy and Gas Chromatography are complementary and they all conclude that the bone black pigment is constituted, mainly, by amorphous carbon and some mineral inclusions. Gas Chromatography and Infrared Spectroscopy show that the pigment is constituted by organic compounds as aromatic hydrocarbons, mainly bitumen. An explanation on how the spots were formed is proposed.     

New techniques for the recovery of small amounts of mature enamel proteins

Currently there are no non-destructive techniques to obtain protein from the dental enamel, the most mineralized tissue in mammals and most resistant to diagenesis, which provides a window to the developing period by means of incremental markings containing proteins. To recover protein, dissolution of powdered enamel is required. Here we tested whether samples obtained by micro-etching of the enamel surface were adequate for protein analysis by MALDI-TOF/TOF mass spectrometry and identification in protein databases. The micro-etch techniques were effective in generating adequate samples for mass spectrometry (from 3 to 13.4 μm superficial enamel), being also highly conservative, since they rendered masses of enamel ranging from 0.1 to 0.4 mg. Using these techniques the separation of proteins by SDS-PAGE was not necessary, and the whole procedure was easier. Results showed successful identification of specific enamel proteins after whole crown superficial etching with 11% EDTA in the case of immature porcine samples, and with 10% HCl in the case of mature human enamel. X- and Y-isoforms of amelogenin, ameloblastin, and enamelin peptides were identified. The new techniques described here allowed the successful recovery of enamel proteins, opening new avenues for the use of enamel protein information in fossil/archeological material, where sometimes little protein is left.     

Spirits of being,spirits of becoming: Bororo shamanism as ontological theatre

Crocker, John Christopher. Vital Souls: Bororo Cosmology, Natural Symbolism, and Shamanism. Tucson: University of Arizona Press, 1985. xiii + 380 pp. including photographs, appendices, bibliography, and index. Foreword by David Maybury‐Lewis. $29.95 cloth.     

The first fossil avian egg from Brazil

Marsola, J.C.A., Grellet-Tinner, G., Montefeltro, F.C., Sayão, J.M., Hsiou, A.S. & Langer, M.C., 2014. The first fossil avian egg from Brazil. Alcheringa 38, 563–567. ISSN 0311-5518.

In contrast to the rich record of eggs from non-avian dinosaurs, complete eggs attributable to Mesozoic birds are relatively scarce. Nevertheless, several well-preserved specimens have been discovered over the last three decades revealing functional and phylogenetic characters that shed light on the breeding strategies of extinct birds. Here we report the first fossil avian egg from Brazil, which was discovered in Upper Cretaceous strata of São Paulo in the southeastern part of the country. The taxonomic identity and structural features of the biomineralized tissues were determined using a combination of Scanning Electron Microscopy, Wave Dispersion Energy analyses and Computed Tomography. These show that the 125.5-μm-thick shell of the 31.4?×?19.5?mm egg incorporates three structural layers of similar thickness with both prismatic and aprismatic boundaries. Close similarity between the Brazilian bird egg and those of enantiornithines from the Upper Cretaceous Bajo de la Carpa Formation (Río Colorado Subgroup) of Argentina advocates affinity with basal Ornithothoraces. Furthermore, coherency of their depositional contexts might imply a compatible preference for breeding and nesting environments.

Júlio Cesar de A. Marsola [juliomarsola@gmail.com], Annie Schmaltz Hsiou [anniehsiou@ffclrp.usp.br] and Max C. Langer [mclanger@ffclrp.usp.br], Laboratório de Paleontologia de Ribeirão Preto, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes 3900, Ribeirão Preto, São Paulo state, 14040-901, Brazil. Gerald Grellet-Tinner [locarnolugano@gmail.com], Centro Regional de Investigaciones La Rioja—Consejo Nacional de Investigaciones Científicas y Técnicas, Entre Ríos y Mendoza s/n, 5301 Anillaco, Argentina; Orcas Island Museum, PO Box 134, 181 North Beach Road, Eastsound, WA 98245. Felipe C. Montefeltro [felipecmontefeltro@gmail.com], Departamento de Zoologia, Universidade Estadual Paulista, Avenida 24A 1515, Rio Claro, São Paulo State, 13506-900, Brazil. Juliana M. Sayão [jmsayao@gmail.com], Laboratório de Diversidade do Nordeste, Núcleo de Biologia, Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Rua do Alto do Reservatório s/n, Bela Vista, Vitória de Santo Antão, Pernambuco state, 52050-480, Brazil. Received 18.12.2013; revised 30.4.2014; accepted 18.5.2014.