An improved PCR method for endogenous DNA retrieval in contaminated Neandertal samples based on the use of blocking primers

Neandertal skeletal remains are usually contaminated with modern human DNA derived from handling and washing of the specimens during excavation. Despite the fact that the distinct Neandertal haplotypes allow the design of specific primer pairs, for instance in most of the mitochondrial DNA (mtDNA) hypervariable region 1 (HVR1), the human contaminants can often outnumber the endogenous DNA, thus preventing a successful retrieval of Neandertal sequences. We have developed a novel PCR method, based on the use of blocking primers that preferentially bind to modern human contaminant DNA and block their amplification, and greatly improve the efficiency of Neandertal DNA retrieval. We tested the method in four El Sidrón Neandertal samples (two teeth and two bone fragments) with different contamination levels and taphonomic conditions, and we have been able to significantly increase the Neandertal yield from figures around 25.23% (5–69.6%) up to 90.18% (75.3–100%).     

Adaptation of heritage architecture in Al Asmakh,Doha: insights into an urban environment of the Gulf

SUMMARY: This paper examines the continuing legacy and occupation of Doha’s vernacular architecture of the early oil period, drawing on techniques from architecture, contemporary archaeology and anthropology. Historical and contemporary inhabitation is examined, as well as the experiences of today’s resident migrant communities. The ongoing significance of Al Asmakh’s vernacular architecture within Qatar’s heritage discourse is considered. Particular attention is given to adaptations undertaken by today’s multinational residents, and the role they play as custodians of Qatar’s architectural heritage. This is a companion piece to the study of vernacular architecture by Eddisford and Carter (2017).     

Book reviews

The Essential Codex Mendoza. By FRANCES F. BERDAN and PATRICIA RIEFF ANA WALT. Berkeley and Los Angeles: The University of California Press, 1997. Pp. xiii, 268, 148.

Cruzados, mártires y beatos: Emplazamientos del cuerpo colonial. MARIO CESAREO. West Lafayette: Purdue University Press, 1995. Pp. xi, 201.

Dineros de ventura: La varia fortuna de la emigración a Indias (siglos XVI‐XVII). By CARLOS ALBERTO GONZALEZ SANCHEZ. Seville: Universidad de Sevilla, 1995. Pp. 277.

La administración como un fenómeno social. La justicia penal de la Ciudad de Quito (1650–1750). By TAMAR HERZOG. Madrid: Centro de Estudios Constitucionales, 1995. Pp. 352.

The Countryside in Colonial Latin America. Edited by LOUISA SCHELL HOBERMAN and SUSAN MIGDEN SOCOLOW. Albuquerque: University of New Mexico Press, 1996. Pp. 295.

Peasants, Politics, and the Formation of Mexico's National State. By PETER F. GUARDINO. Stanford: Stanford University Press, 1996. Pp. viii, 319.

Poder terrateniente, relaciones de producción y orden colonial. By EDUARDO AZCUY AMEGHINO et al. Buenos Aires: Fernando García Cambeiro, 1996. Pp. 259.

Storms Brewed in Other Men's World's: The Confrontation of Indians, Spanish, and French in the Southwest, 1540–1795. By ELIZABETH A. H. JOHN. Norman: University of Oklahoma Press, [1975] 1996. Pp. 805.

The World Upside Down: Cross‐Cultural Contact and Conflict in Sixteenth‐Century Peru. By SUSAN ELIZABETH RAMIREZ. Stanford: Stanford University Press, 1996. Pp. 234.

Architecture and Power in the Ancient Andes: The Archaeology of Public Buildings. By JERRY D. MOORE. Cambridge: Cambridge University Press, 1996. Pp. xiii, 256.

Las crónicas y los Andes. By FRANKLIN PEASE G. Y. Mexico: Fondo de Cultura Económica, 1995. Pp. 632.

The War with Spain in 1898. By DAVID F. TRASK. Lincoln: University of Nebraska Press, [1981] 1996. Pp. xiv, 654.

The Village and the Outside World in Golden Age Castile. Mobility and Migration in Everyday Rural Life. By DAVID E. VASSBERG: Cambridge: Cambridge University Press, 1996. Pp. xvii, 253.     

Rethinking ‘Bellarmine’ contexts in 17th-century England

SUMMARY: Archaeologists have identified and examined ‘Bellarmine’ vessels for many years. Their accumulated research has proven instrumental in providing abundant information about the vessels’ dates, styles and distribution. Seldom, however, have archaeologists considered the deeper significance or the major terms used for the vessels. This paper investigates the meanings of various terms and argues for a greater understanding of the relationships between the bearded-man images on the vessels and two eras in which the terms appear — the past and the present. The concept of conceptual frames assists with the interpretation.     

El rito bautismal y las imágenes pintadas en la iglesia de Curahuara de Carangas

In the baptistery of the church of Santiago de Curahuara de Carangas, built at the end of the 16th century, a complex mural painting program was executed in the last quarter of the 18th century. The present text intends to establish the links between the scenes painted and the rite of baptism. We do not see a direct or evident relationship in a didactical or performative sense. The images are there to be potentially activated during the liturgical ceremony in order to reveal the efficacy of the sacrament. We have the advantage of working with a set of images that remain in their original location. Therefore, this essay proposes a potential relationship among gesture, words and paintings, as they could have been related in the 18th century. In the analysis, we put into dialogue the resonances intended by the Church and the reception by the indigenous community.     

Microanalytical study of copper ores from the Chalcolithic settlement of São Pedro (Portugal): Copper production in the south-western Iberian Peninsula

Copper ores recovered at the third millennium bce settlement of São Pedro, Portugal, were characterized by micro-Raman spectroscopy, micro-energy dispersive X-ray spectrometry (micro-EDXRF) and micro-particle-induced X-ray emission spectrometry (micro-PIXE). The collection shows the common presence of secondary copper minerals (malachite, pseudomalachite and libethenite) combined with iron oxyhydroxides (hematite and goethite), while arsenic-rich minerals are absent and, therefore, can be excluded as the source of arsenical copper. Overall, these copper ores suggest a primitive technology involving the exploitation of the superficial zone of ore bodies and reliant on the fortuitous finding of arsenic-rich sources to produce the arsenical copper alloy frequently present among metallic collections of the Iberian Peninsula.     

The Survival of Words: Revolution and Class Struggle

How did Kirchnerismo, initially a motley coalition of progressive Peronists and born-again Menemistas and Duhaldistas trying to scrape together some form of governability from the 2001 default, turn into one of the ‘pink tide’ governments most virulently hated by the Right? Why did long-forgotten concepts and terminologies from the epic past of revolutionary struggle suddenly resurface in the context of what, at least from the viewpoint of the autonomist Left, was hardly more than a mildly redistributive administration of scarcity, aided by the post-millennial commodity boom? In this intervention, historian Javier Trímboli urges us to take seriously some of the discursive anachronisms that flourished during Néstor and Cristina Kirchner’s periods in office (2003–2015), which, he suggests, rather than merely a form of discursive cover for clientelist politics, may have been symptomatic of the way in which some of the foundational fault-lines of modern Argentine society continue to shape political identities, styles and forms of struggle in the present.