Bone preservation and DNA amplification

The use of ancient DNA has increased during the past two decades in several scientific disciplines. However, the underlying mechanisms of DNA degradation in bone tissue are poorly understood. Here we address the importance of hydroxyapatite and collagen for DNA preservation in bone. We used two series of bones and teeth, one set of modern experimentally degraded bovid bones and one set of ancient horse bones/teeth. From these samples, we measured crystallinity, DNA presence and extracted collagen. The mtDNA fragments, parts of cytochrome b and the D–loop were amplified and sequenced. Our results show that presence of DNA was strongly related to the crystallinity in the hydroxyapatite and to the amount of collagen. This suggests that the hypothesis that hydroxyapatite has a crucial role in DNA preservation in calcified tissue is valid; and hydroxyapatite and collagen can be used to indicate whether DNA is present in the material. This is what would be expected if DNA is adsorbed to and stabilized by hydroxyapatite in calcified tissue, and collagen is part of the complex system that preserves DNA in bone tissue. Further, since collagen is the preferred material for radiocarbon dating, such bones may be a starting–point for a DNA analysis.     

Metelkova as Autonomous Heterotopia

This article examines conflicts concerning urban space, focusing on relationships between autonomous space and neoliberal urbanism through the empirical example of the cultural centre AKC Metelkova Mesto in Ljubljana, Slovenia. Through a thematic discourse analysis of activist interviews and collective statements by activist groups connected to Metelkova, research questions concern how urban conflict is constructed from the vantage point of autonomous space; what role autonomous space is assigned in relation to such conflict; and how tensions and antagonisms within the autonomous space can be understood. Theoretically we engage in a reinterpretation of the notion of heterotopia in conjunction with critical urban theory, analysing Metelkova as an autonomous heterotopia. Further, we argue that theoretisations of autonomous spaces need to consider experiences from Central and Eastern Europe, in which the conditions are shaped and constructed in conjunction with particular configurations of abruptly implemented neoliberal governance and the rise of the authoritarianism.     

Confidence Building in Local Planning and Development. Some Experience from Norway

Can local confidence building and cooperation strengthen local communities and have a positive influence on local development? This is the main question in this article. In the first part of the article the conditions for confidence building, mobilization, self-development and self-confidence in local planning are presented. A planning process called strategic and mobilizing planning which is built on these conditions is described. This planning or development process is drawn as an infinite spiral with an increasing 'radius'. As examples of such local confidence building and mobilization processes the cases of 'Nordvest Forum' and 'Cooperation in Haram commune' are presented and discussed. Nordvest Forum is a collaborative activity owned by competing companies in the region, which have recognized that they have a common problem related to recruitment and training of leaders. This has lead to new activities and new cooperation especially related to management training and cooperation. The collaboration between private and public institutions in Haram is an example where common challenges for companies and community were recognized. This process started as a concrete activity regarding recruitment of skilled workers with participation from a few but nevertheless important persons in the private and public sectors. The learning and confidence developed as a result of this cooperation lead to new and broader activities concerning the future of the community with many more participants. In the conclusion some practical advice is given for community planning and confidence building, informed by the theoretical discussion and the empirical examples in the article.     

Sheep wool in Bronze Age and Iron Age Europe

This study presents the results of a series of wool measurements from Bronze Age and Iron Age skins and textiles from Hallstatt, and Bronze Age textiles from Scandinavia and the Balkans. A new method of classification that was set up and applied on mostly mineralised Iron Age material has now been applied to a large body of non-mineralised material from the Bronze and Iron Ages. Three types of microscopes were used and their advantages and disadvantages assessed. The results of the investigation cast new light on sheep breeding and fibre processing in prehistoric Europe, and suggest that different sheep breeds existed in Bronze Age Europe.     

Planning Analysis and Political Steering with New Public Management

Interviews with members of the Norwegian national assembly's Standing Committee for Transport and Communications were conducted in 1995, 1997, 2001 and 2004. The politicians were asked about the usefulness of analytic input that planners contribute to national plans, first the Norwegian Road and Road Traffic Plan and later the National Transport Plan. Questions related to steering, coordination, delegation and the inclusiveness of the planning process were also posed. Changes in the politicians' attitudes to types of analytic planning input are outlined. Furthermore, the last round of interviews sheds light on the apparent paradox that parliamentarians accept delegation of highway investment decisions despite their profound scepticism regarding the cost–benefit analyses and impact calculations that are essential to management by objectives and results (MBOR), which was to give them continued political control despite delegation. Another conundrum is the parliamentarians' willingness to renounce the right to decide over most highway projects, even if these were seen as politically important, in order to obtain a strategic overview which they have been unable to use for changing the priorities set by the Government. The political concentration on strategic steering, the concomitant delegation, and MBOR are central components of New Public Management.