Deficiencies and challenges in the study of ancient tuberculosis DNA

We explore the standards of research and reporting needed to justify the destructive analysis of archaeological human bone for biomolecular studies of ancient tuberculosis (TB). Acceptable standards in osteological interpretation have been met in some biomolecular papers, but there are also cases where insufficient care has been taken in distinguishing between pathognomonic lesions and those that are ‘consistent with’ a diagnosis of TB. Some biomolecular studies have failed to recognize that archaeological bones might be contaminated with environmental mycobacteria whose DNA could give rise to false positives in polymerase chain reactions directed at members of the Mycobacterium tuberculosis complex. The difficulties of applying spoligotyping to ancient DNA have also been underestimated and conclusions drawn from such analyses are often weakly supported. Assumptions that mycobacterial DNA preserves better than human DNA, and that contamination with modern DNA is less of a problem, has led in some cases to a laxity in research standards with insufficient attention paid to the need to authenticate ancient DNA results. We illustrate our concerns by reference to a recent paper reporting biomolecular detection of ancient TB DNA in skeletons from the eastern Mediterranean Neolithic settlement of Atlit-Yam. We are unconvinced that the skeletal evidence presented in this paper gives sufficient indication of TB to warrant destructive analysis, and we are concerned that during the biomolecular part of the project inadequate attention was paid to the possibility that results might be due to laboratory cross-contamination or to amplification of environmental mycobacterial DNA present in the bones.     

Stringing Together Cowrie Shells in the African Archaeological Record with Special Reference to Southern Africa

Journal of Archaeological Method and Theory - Archaeological explorations of the meaning of ‘trade objects’, such as glass beads and cowrie shells, remain hampered by theoretical and...     

The Importance of Multiple 14C Dates from Significant Archaeological Contexts

Radiocarbon (¹⁴C) dates are the most important means for determining the age of Holocene archaeological deposits. The theoretical physical basis of this method is by now unassailable, having been consistently tested and refined over two generations. However, the means by which this method has been applied and the interpretation of these results remain as key issues, particularly for complex archaeological discoveries that substantially affect our understanding of world prehistory and social evolution. Many factors can produce uncertainty or variation in the ¹⁴C concentrations of samples, even those that have been selected from the same archaeological context or event. A number of recent studies have also addressed the ways in which ambiguities and irregularities in the ¹⁴C calibration curve can affect the interpretation of archaeological dates and temporal patterns. Of greatest concern, however, is a growing practice of using only one or two samples to date a significant prehistoric context or event. The date of these events, usually relative to other human activities, often holds important theoretical implications for evolutionary anthropology and related disciplines. In this article, we demonstrate that such a practice is rarely adequate or acceptable. Rather, proper procedure requires a suite of dates that permit statistical verification that the deposit or event itself is being correctly dated. We present a detailed case study that highlights the importance of analyzing multiple samples of ¹⁴C from significant archaeological contexts.     

Kinship between burials from Grave Circle B at Mycenae revealed by ancient DNA typing

The richness of the burials in Grave Circle B at Mycenae, Greece indicates that the 35 people interred there held elite status during their lifetimes 3500 years ago. It has been speculated that the groups of burials represent different dynasties or branches of the same family. To test this hypothesis, we carried out an exhaustive ancient DNA (aDNA) study of 22 of the skeletons. We were unable to identify nuclear aDNA in any specimen, but we obtained authentic mitochondrial aDNA sequences for four individuals. The results were compared with facial reconstructions and interpreted within the archaeological context represented by the organisation of the graves and the positions of the burials within the graves. We conclude that the contemporaneous male Γ55 and female Γ58 skeletons, which both possess the UK mitochondrial haplogroup, were brother and sister. The implication is that Γ58 was buried in Grave Circle B not because of a marital connection but because she held a position of authority by right of birth. The results illustrate the difficulty in using aDNA to study kinship relationships between archaeological specimens, but also show that aDNA can advance understanding of kinship when used to test hypotheses constructed from other evidence.     

Mitochondrial DNA haplotypes of Devensian hyaenas from Creswell Crags,England

Spotted hyaena (Crocuta crocuta) remains have been recovered from British Middle and Upper Pleistocene sites at intervals within the period 700–730 ka BP. Morphological studies have suggested that hyaenas of the Last Interglacial sensu stricto (Ipswichian: Marine Isotope Stage [MIS] 5e, 130–115 ka BP) and Last Glacial (Devensian: MIS 3, 61–24 ka BP) were two distinct populations, the Ipswichian hyaenas becoming extinct in Britain during MIS 5 and the Devensian ones arriving via a subsequent migration from continental Europe. However, the apparent presence of hyaenas in later MIS 5 deposits has led to the alternative suggestion that there was a southern relict population from which the Devensian hyaenas originated. We obtained ancient DNA (aDNA) sequences from four Devensian hyaena specimens from Creswell Crags, Derbyshire, dated to around 45 ka ¹⁴C BP. Each of these four specimens belonged to the mitochondrial DNA (mtDNA) clade A. This clade is not thought to have been present in Europe until ~360 ka BP, after the initial arrival of hyaenas in Britain. The DNA results, therefore, suggest that there were at least two waves of hyaena dispersals into Britain. The results are consistent with the repeated dispersals into Britain of another Pleistocene social carnivore, Homo sapiens.     

Breaking Ground: Hoes in Precolonial South Africa—Typology,Medium of Exchange and Symbolic Value

In sub-Saharan Africa, every cultivator of the soil in a precolonial, agriculturist society needed a digging implement, and this normally took the form of a hoe with an iron blade hafted on a wooden handle. This paper marks the first attempt to document the surprising variety of hoe blade styles and map their regional distributions within South Africa. While the primary function of the hoe—this key item of material culture in African farming communities—was to cultivate the soil, the large quantity of metal and therefore labour required to make a hoe blade accorded it great value in the years before European industrial copies flooded the market. The hoe was an important item in trade networks, in marriage transactions and in a number of symbolic contexts associated with individual and collective ancestry. This biographical approach to hoe exchange, use and recycling reveals the complex significance of this tool in the lives of precolonial farmers. In particular, we are able to show that hoes could in some circumstances be accorded the same values as cattle in marriage exchange.     

Counting eyeballs,soundbites and ‘plings’: arts participation,strategic instrumentalism and the London 2012 Cultural Olympiad

This article examines cultural participation, its metrics and ‘drivers’ as they are defined through cultural programming for the London 2012 Olympics. The meanings and interpretation of these terms are considered by examining the development of an evaluation framework for the We Play programme in the North West of England, an initiative funded by Legacy Trust UK and part of the London 2012 Cultural Olympiad. It argues that in spite of the dissonance between arts and sports within Olympics programmes and claims of the deleterious impact on arts funding, particular within the regions, London 2012 has engendered creative programming which strategically deploys the Cultural Olympiad to satisfy local cultural policy objectives as well as meeting broader interests in ‘legacy’ from the Games. Such ambitions require the development of appropriate methodologies for understanding arts participation and engagement for the purpose of evaluation and evidence-based policy making, a particular challenge for such a complex range of activities, sites and settings for arts participation.