Landscape bioarchaeology at Pacatnamu,Peru: inferring mobility from δC and δN values of hair

Stable carbon- and nitrogen-isotope ratios were measured for segments along the shafts of hair from eight individuals from the site of Pacatnamu, located in the Jequetepeque Valley on the north coast of Peru. All are from known grave contexts dating from Moche (ca. 450–750 A.D.) to Lambayeque periods (ca. 900–1100 A.D.). The mean δ¹³C and δ¹⁵N values of hair segments from individuals are comparable to those of bone, and demonstrate increased consumption of marine resources in the Lambayeque times relative to the Moche period.     

Re-investigating fish consumption in Greek antiquity: results from δC and δN analysis from fish bone collagen

One of the frequently encountered issues in ancient Greek dietary reconstructions through isotope analyses has been the apparent unimportance of fish protein in human diets. The significance of this observation is amplified by the abundant ichthyofaunal remains, iconographic evidence and literary information on fish and fishing, pertaining to almost all sites and time periods of Greek antiquity. In this project, we measured for the first time isotopes from a large number of fish bones from Greek sites dating from the Mesolithic to the Classical times, aiming to investigate whether this absence is an artefact of the methodology or whether it reflects a reality of restricted fish consumption. Results show that regional trends are stronger that temporal ones in fish isotope values. The range of values overlaps with terrestrial resources, making it difficult or impossible to reject fish consumption based on isotope data alone. This variability proposes a reconsideration of the amount of fish in ancient Greek diets specifically for each site and amplifies the importance of interdisciplinary studies, especially for regions with variable ecological resources.     

Modern plant δN values reflect ancient human activity

We have observed circumstances in which the ancient human activities that formed archaeological sites have left a strong imprint on the stable nitrogen isotopic ratio of the plants currently growing there. There is apparent meter-scale spatial variability and the effect can persist for at least several centuries. To our knowledge, this isotopic effect has not previously been reported. Here, we introduce this phenomenon and begin to consider its potential as an analytical tool in archaeological reconstruction.     

Correlation between modern plant δN values and activity areas of Medieval Norse farms

This further nitrogen isotopic study of Greenlandic Norse archaeological sites provides new evidence of the affect of past human activity on modern plant δ¹⁵N values. Grass samples were taken from a number of spatially defined features at four Norse farms and one site interpreted to be a seasonally occupied herding station (sæter). At the farms, un-naturally high δ¹⁵N values were found for plants from each sampled feature, including those from the likely infields and a churchyard. At the sæter no strong isotopic effect was expected and none was found. Comparing the data from the farms we found that the average plant δ¹⁵N values were similar for features that were functionally alike. We conclude from these observations, that the unusually high plant δ¹⁵N values reflect the strong conservation of the isotopic composition of the nitrogenous materials deposited in the past, and the magnitude of the values reflects the amount of this material admixed in the soil. The results confirm previous exploratory studies of this isotopic effect and further demonstrate its potential value as an archaeological tool.     

The pilgrimage economy of Early Roman Jerusalem (1st century BCE–70 CE) reconstructed from the δN and δC values of goat and sheep remains

Religious and historical sources suggest that pilgrimage formed a major source of Jerusalem's economy during the Early Roman period due to the Temple's role as a religious and judicial center for the Jewish diaspora. Until now, this assertion has been supported by little material evidence. In this study, the carbon and nitrogen isotope values of local arcahaeological and, modern wild herbivores from known environments were used to determine the environmental origins of domesticated sheep and goat that were traded and consumed in Early Roman Jerusalem. Pinpointing the environmental origins of these herd animals can determine if they were raised in specialized farms in the vicinity of Jerusalem, brought to the city by local pilgrims, or were part of organized importation of sacrifice animals from desert regions that lie beyond the boundaries of the province of Judea. The results indicate that at minimum 37% of the goat and sheep consumed in Jerusalem during the Early Roman period were brought from desert regions. The inter-provincial importation of animals to Jerusalem to meet high demands for sacrifice by pilgrims is the first material evidence for large scale economic specialization in the city. Furthermore, the results imply that desert animals were further marketed for domestic use in contemporaneous farm sites out of Jerusalem.     

Introducing δSr analysis in archaeology: a demonstration of the utility of strontium isotope fractionation in paleodietary studies

Isotopic methods are widely used in archaeology to investigate paleodiet. Here, we present a new method to identify trophic level in archaeological human populations and to investigate paleodiet. We demonstrate that strontium isotope compositions (reported as δ^88/86Sr) vary in a mass-dependent manner with increasing trophic level and can elucidate paleodiet in archaeological human populations. We present new mass-dependent strontium isotope data from tooth enamel and bone from individuals buried during the Late Intermediate Period (c. AD 1000–1300) in the large cemeteries of Chiribaya Alta, Chiribaya Baja, San Gerónimo, and El Yaral in the Ilo and Moquegua Valleys of southern Peru. We compare these data to radiogenic strontium isotope data (⁸⁷Sr/⁸⁶Sr) and light stable isotope data (δ¹⁵N_col and δ¹³C_col) from the same individuals to investigate geologic variability in strontium sources as well as marine food consumption among the Chiribaya. Our results demonstrate the utility of measurements of strontium isotope fractionation as a new tool for archaeological investigation of paleodiet. Importantly, this new technique can be used to generate paleodietary (δ^88/86Sr) and paleomobility (⁸⁷Sr/⁸⁶Sr) data from the same specimen, minimizing destructive analyses of invaluable archaeological material, and provides a new way to examine paleodiet through hydroxyapatite, which is particularly important when collagen is poorly preserved.     

Impact of charring on cereal grain characteristics: linking prehistoric manuring practice to δN signatures in archaeobotanical material

Systematic use of animal manure has been demonstrated to be detectable in the plant δ¹⁵N value but evidence of manure affecting isotopic composition is mainly based on studies of fresh plant material. These findings can potentially be applied to archaeobotanical assemblages and thus provide information about prehistoric manuring practice. Prehistoric grains are generally found in a charred state of which the exact charring conditions are unknown but most likely often multifarious. In this study we examined the influence of grain weight and a range of charring conditions with regards duration, temperature, oxygen availability, and manuring. The charring was applied to emmer, spelt and naked barley with assessment of weight loss, N concentration and δ¹⁵N. There were only small and non-systematic variations in δ¹⁵N in relation to grain weight class. We also found that charring did not distort δ¹⁵N in either a systematic or substantial way and conclude that manuring most likely will be detectable in archaeobotanical charred grains. As certain within-grain variability in δ¹⁵N existed, especially in the intensively manured grains, the resolution of this kind of information should be carefully considered. However, despite attempts to deliberately tamper and distort the grain δ¹⁵N signature, the changes observed in this study were too small to be of any consequences for the archaeobotanical applicability of the method. Thus the isotope method offers unique evidence about prehistoric manuring practice.     

Seasonality and season of birth of modern and late Neolithic sheep from south-eastern France using tooth enamel δO analysis

Stable oxygen isotopic analysis of tooth enamel was used to investigate seasonality and season of birth in sheep. Analyses were performed on the teeth of eight modern sheep bred at the Carmejane farm in south-eastern France and born in different periods of the year. This reference data set confirms that oxygen isotopic records in the second and third molars can be used to differentiate between sheep born in late winter and those born in early autumn. The results show that inter-individual variability in the timing of tooth formation is low for the second lower molar and more pronounced for the third lower molar in this population. The duration of the process of enamel mineralization has been estimated at approximately six months. The teeth of four sheep and two goats from the late Neolithic site of Collet-Redon in south-eastern France were analyzed using the same methodology. At this site, lambing occurred between the late winter and early spring. A slight difference appears in the seasonal sequence recorded in sheep and goat, although it cannot be determined, whether this is due to different timing of tooth development, or an actual shift in the birth period. From the distribution of slaughter ages and birth period, it is suggested that domestic caprines were killed throughout the year and that Collet-Redon was probably a permanent settlement.     

The variation in δ13C values in bone collagen for two wild herbivore populations: Implications for palaeodiet studies

The δ¹³C values for un-defatted bone gelatin of individual members of wild populations of mule deer (Odocoileus hemionus hemionus) and field vole (Microtus townsendii) have been measured to determine the variability within each population. Means and standard deviations for the mule deer sample (N=55) and vole sample (N=22) are −20·6±0·5‰ and −23·2±0·6‰, respectively. The distributions of δ¹³C values for each of these populations are normal and we observed no correlation in δ¹³C values with age or sex.     

Hardwood ash nixtamalization may lead to false negatives for the presence of maize by depleting bulk δC in carbonized residues

Among the multiple proxies for detecting maize in precontact economies is the use of δ¹³C analysis of carbonized residues from ceramic cooking vessels. Although maize horticulture was widely established in Eastern North America (ENA) by A.D. 1000, there are carbonized residues from ceramic assemblages after this date that lack the elevated δ¹³C values indicative of the presence of maize. This may be due to the true absence of maize, or other factors including the masking of maize. Prior experimental research by Hart et al. demonstrated that the addition of C₃ plants or consumers to two part mixes with maize can mask maize presence even when maize is the dominant ingredient. Here we investigate the effect of alkali processing of maize (nixtamalization) on δ¹³C using the widespread ENA process of boiling maize kernels with wood ash, a C₃ product, to create hominy. Our experiments test whether or not the process of hardwood ash nixtamalization can mask the presence of maize in adhering carbonized residues by depleting δ¹³C values, and whether there is a reciprocal δ¹³C enrichment effect on the hardwood ash employed in nixtamalization. Overall, there is substantial δ¹³C depletion of residues when maize is cooked with hardwood ash, and hardwood ash cooked with maize shows the reciprocal enrichment. Therefore, the depleted values after the adoption of maize may be false negatives due to the nixtamalization process.     

Paleodiet in northern Chile through the Holocene: extremely heavy δN values in dental calculus suggest a guano-derived signature?

Dental calculus extracted from the teeth of 28 prehistoric human skeletons from coastal and valley archaeological sites in northern Chile that date from the Archaic period (∼2300 BC) to the Late Intermediate period (AD 1476) was analyzed for stable isotope (δ¹⁵N, δ¹³C) compositions. δ¹⁵N compositions were extremely heavy (+17.8 to +33.1‰), but comparable to some studies using conventional human biomaterials (hair, nail, muscle) at other prehistoric sites in northern Chile. There was a negative correlation between δ¹⁵N and δ¹³C for coastal sites, but a positive correlation for valley sites. Results for the valley sites point to a diet that was influenced by marine resources throughout all time periods. The unusually heavy δ¹⁵N values for the coastal sites require a dietary component with a δ¹⁵N composition significantly heavier than that of marine resources. The hyper-aridity at the study area (mean annual rainfall of 0.5–0.6 mm/year) is a likely contributing factor to the production of heavy δ¹⁵N values, but is unlikely to account for the heaviest δ¹⁵N values (>+30‰). One possible explanation for the heaviest δ¹⁵N values is that dietary components were impacted by isotopically-heavy guano, which is abundant in the region. Guano may have been used as a fertilizer during crop cultivation at the onset of the Formative period, continuing through the Historic period after Spanish contact. The indirect impact of guano from abundant bird habitats in the region may have influenced wild foodstuffs harvested throughout the pre-agricultural period. Results provide support for the utility of dental calculus as a proxy for obtaining stable carbon and nitrogen isotope signatures for use in paleodietary studies.     

A bioarchaeological approach to the Iron Age in Switzerland: stable isotope analyses (δ<Superscript>13</Superscript>C, δ<Superscript>15</Superscript>N, δ<Superscript>34</Superscript>S) of human remains

In Switzerland, a large number of Iron Age burial sites were found in the last century. Changes in living conditions and socio-cultural behavior may have occurred over time and space and could be reflected in the dietary habits, social stratigraphy within populations and migration patterns. This study attempts to shed light on these aspects with the application of stable isotope analyses. Human remains from 11 different burial sites (n?=?164) in the area of today’s Swiss Plateau and Swiss Alpine regions were investigated. Temporal and geographical variations as well as sex and age-related dietary differences were analyzed through isotopic studies (δ¹³C, δ¹⁵N, and δ³⁴S). In total, the data of 129 individuals could be evaluated. Highly significant differences between the burial sites were found, with higher δ¹³C and δ¹⁵N values in the Alpine regions. Cultural and/or climatic changes as well as the different geological conditions might have led to distinct patterns of crop cultivation and animal husbandry and consequently to significantly different dietary habits in the Plateau and the Alpine regions. The data indicate a higher intake of millet and animal protein including early dairy production in the southern regions, probably influenced by the Mediterranean world. Cultural exchange between geographical regions might have been facilitated by migration during the Iron Age as suggested by the δ³⁴S.     

The effect of parity status on δN: looking for the “pregnancy effect” in 18th and 19th century London

This study examined the effect of parity status on δ¹⁵N using the well-characterised 18th and 19th century skeletal collection from the crypt of Christ Church, Spitalfields, London. We tested whether the cumulative effect of multiple pregnancies and breastfeeding could significantly reduce female δ¹⁵N values compared to males. The results from stable isotope analysis of 92 adult ribs show that the population of Spitalfields had relatively little variation in diet, compared to contemporary urban populations, and had abundant animal and marine protein. We were able to rule out any effect attributable to socio-economic status, date or age at death on the stable isotope ratios. There were no significant differences in δ¹⁵N due to parity status, nor were there any differences between males and females. Models of collagen turnover rates in ribs suggest that the effect of δ¹⁵N depletion due to pregnancy would be undetectable except in ideal circumstances, where bone of the optimal turnover period was sampled, and in cases where multi-parous individuals died shortly after parturition.     

Pastoralist Mobility in Bronze Age Landscapes of Northern Kazakhstan: 87Sr/86Sr and δ18O Analyses of Human Dentition from Bestamak and Lisakovsk

ABSTRACT

The role of migration and mobility of people across the steppe has often been cited as key to Bronze Age developments across Eurasia, including the emergence of complex societies in the steppe and the spread of material culture. The central Eurasian steppe (CES) is a focal point for the investigation of the shifting nature of pastoral societies because of the clear transition in archaeological patterning that occurred from the Middle (MBA) to Late Bronze Age (LBA). The spread of LBA (1700–1400?cal BC) Andronovo cultural materials found across wide swaths of the steppe provide indirect evidence for broad scale interactions, but the degree to which people moved across the landscape remains poorly understood. This study takes a first step into documenting human movement during these critical periods through strontium (⁸⁷Sr/⁸⁶Sr) and oxygen (δ¹⁸O) isotopic analyses of tooth enamel recovered from human individuals buried in the cemeteries of Bestamak (MBA) and Lisakovsk (LBA) in northern Kazakhstan. Strontium isotope results, referenced against the distribution of contemporary bioavailable strontium in the vicinity of both sites, suggest local communities engaged in small-scale mobility with limited ranges. Reduced strontium and oxygen isotopic variation visible in humans from Lisakovsk suggests mobility decreased from the Middle to Late Bronze Age likely indicative of a shift in resource and landscape use over time.     

Seasonality of birth and diet of pigs from stable isotope analyses of tooth enamel (δO, δC): a modern reference data set from Corsica,France

Since domestication during the mid 11th millennium BP in the Near East and the 8th millennium BP in China, the pig has played an important role in human diet and economy. The objective of this study is to evaluate the potential of stable oxygen and carbon isotopes sequential analyses of pig tooth enamel to investigate pig seasonality of birth and diet, as these are important parameters for the reconstruction of pig herding strategies in ancient societies.     

Stable isotope insights (δ18O, δ13C) into cattle and sheep husbandry at Bercy (Paris,France, 4th millennium BC): birth seasonality and winter leaf foddering

Abstract

Bercy is a prehistoric village sited by the Seine river (Paris, France), whose main period of occupation was dated to the very beginning of the 4th millennium BC. The animal subsistence economy relied heavily on cattle husbandry, complemented by other species including sheep. Cattle and sheep isotopic history (δ¹³C, δ¹⁸O) was investigated at the seasonal scale, through sequential sampling in tooth enamel, providing new insights into seasonality of birth and diet. Sheep were lambing in mid-spring, only slightly later than expected from what is observed nowadays in temperate Europe at similar latitude. Cattle were born over a period spanning approximately six months, which was an unexpected result compared with a two to three months calving period in free-ranging cattle populations. The extension of the calving period might have been related to seasonal food supplementation. Some cattle and some sheep fed on a ¹³C-depleted resource in winter, potentially leafy fodder. A direct consequence of an extended calving period would be the availability of cow milk, which would have covered the whole year at Bercy. This is important information in a context where the exploitation of cattle milk by the human community was highly suspected from the demographic management of the herd.     

Probing dietary change of the Kwädąy Dän Ts'ìnchį individual,an ancient glacier body from British Columbia: II. Deconvoluting whole skin and bone collagen δC values via carbon isotope analysis of individual amino acids

The remains of the Kwäd?y Dän Ts'ìnch? individual, a frozen male human, were recovered from a retreating glacier within the Tatshenshini-Alsek Park in British Columbia in August 1999. In order to provide information on both the geographical origin of this individual and low long he spent in the remote interior region prior to his death, molecular analysis and compound-specific carbon isotope analyses were performed on individual amino acids purified from his skin and bone. Gas chromatographic quantification of constituent amino acids of both tissues revealed a molecular distribution characteristic of collagen, dominated by glycine and to a lesser extent proline, hydroxyproline and alanine. Chiral gas chromatography indicated that protein preservation in both tissues was exceptional. Carbon isotope analysis of a faunal assemblage from an earlier prehistoric site from southern British Columbia provided reference dietary amino acid δ¹³C values for terrestrial (deer and domestic dog) and marine species (salmon and sealion), showing clear separation in all amino acids, particularly glycine which was extremely ¹³C-enriched in the marine animals. The distinction between terrestrial and marine organisms was increased by exploring Δ¹³C_{Glycine-Phenylalanine} values (6.6 ± 0.6‰ and 15.0 ± 2.1‰, respectively), which were higher in the latter by approximately 8‰, mirroring the increased δ¹⁵N_{Bulk collagen} values observed for the marine animals (R² = 0.78; p < 0.001). The Kwäd?y Dän Ts'ìnch? individual's bone had a similarly elevated Δ¹³C_{Glycine-Phenylalanine} value of 15.6 ± 1.0‰, indicating his extreme reliance on marine dietary resources throughout early life. The skin amino acid δ¹³C values were consistently lower than those observed for bone, with a concurrently lower Δ¹³C_{Glycine-Phenylalanine} value of 12.7 ± 0.9‰. The shift between the carbon isotope composition of bone (long-term diet) and skin amino acids (short-term diet) confirmed a sudden divergence away from marine food sources in the last months of life, consistent with his discovery 80 km inland.     

The influence of manuring on stable isotopes (δ<Superscript>13</Superscript>C and δ<Superscript>15</Superscript>N) in Celtic bean (<Emphasis Type="Italic">Vicia faba</Emphasis> L.): archaeobotanical and palaeodietary implications

This paper examines the impact of animal manure on δ¹⁵N and δ¹³C values in a legume, Celtic Black broad bean (Vicia faba). In a field experiment, V. faba was cultivated in plots treated with farmyard manure and pure sheep manure. The results indicate that highly intensive manuring can increase δ¹⁵N values in beans, stems, leaves and pods. In comparison, manuring had a relatively small impact on δ¹³C values. In terms of palaeodietary reconstructions, the high δ¹⁵N values in very intensively manured beans (+3?‰) are equivalent to the trophic-level effect. Based on the experimental results, it is suggested that high δ¹⁵N values in archaeobotanical remains of V. faba may be attributable to small-scale cultivation with intensive manuring.