Ethnoarchaeological study of phytolith assemblages from an agro-pastoral village in Northern Greece (Sarakini): development and application of a Phytolith Difference Index

Phytolith assemblages are analysed in an ethnographic agro-pastoral community in Northern Greece. A new method for analyzing the data, combined with the concentrations of phytoliths per gram sediment, helps to differentiate diverse uses of space in the village. The Phytolith Difference Index (PDI) contrasts the phytolith assemblages in sediment samples from the region around the village least affected by human activities with those in the village and its immediate surroundings. The PDI reveals that many of the samples are dominated by the input of the stalks of the domestic cereal, rye, which is used for food, animal fodder and roof thatching. The PDI also differentiates between dung from mules or donkeys with dung from free ranging cows and goats. Activity areas analysed include storage areas, stabling areas, animal enclosures, floors from living areas that were repeatedly swept, hearths and open areas between structures. The combined use of the PDI, together with phytolith concentrations and phytolith morphotype analyses, may prove to be useful for deciphering activity areas in archaeological sites of not only agro-pastoralists, but also pastoralists and hunter–gatherers.     

Rapid phytolith extraction for analysis of phytolith concentrations and assemblages during an excavation: an application at Tell es-Safi/Gath,Israel

A rapid phytolith extraction procedure is described, that allows phytolith concentrations and morphotype assemblages to be analyzed within hours. This procedure enables the results of these analyses to be used during an archaeological excavation, in order to better understand how plants were used. The new procedure was tested using a standard phytolith extract and two experimental phytolith-sediment blends and found to be both accurate and precise. The reliability of partial slide counting was evaluated and found to be as accurate and precise as existing phytolith quantification methods. The new extraction and counting procedures were applied to an archaeological site, Tell es-Safi/Gath, Israel. The results demonstrate how information on phytolith concentrations in sediments that are available from one day to the next, can be used during an excavation to more effectively document the local features of interest and obtain better information.     

The use of multiple discriminant analysis in classifying prehistoric phytolith assemblages recovered from cooking residues

In the investigation of the dispersal of maize (Zea mays ssp. mays) from south-central Mexico to the northern and southern limits of agriculture in the Western Hemisphere archaeologists and paleoethnobotanists are increasingly turning to the microbotanical record. Recent analysis of phytolith assemblages from charred cooking residues on pottery sherds in central New York recovered using 209 rondel phytolith variables has identified maize as early as 2270 ± 35 B.P. In this article we use discriminant analysis to re-classify these rondel phytolith assemblages resulting in only seven variables. The results are consistent with those achieved earlier using many more variables and a less formal statistical approach in terms of classification and in similarity of the original and reduced data matrix as seen by the Mantel test and cluster analyses.     

Food and social complexity at Çayönü Tepesi,southeastern Anatolia: Stable isotope evidence of differentiation in diet according to burial practice and sex in the early Neolithic

The identification of early social complexity and differentiation in early village societies has been approached in the past most notably through the evaluation of rituals and architectural layouts. Such studies could be complemented by an approach that provides data about everyday behaviours of individuals. We took 540 human and animal bone samples for stable carbon and nitrogen isotope analysis from the Neolithic site of Çayönü Tepesi in southeastern Anatolia. The inhabitants at this site chose to bury their dead in two different ways at different times during its occupation: beneath the floors of their houses, but also inside a public mortuary building known as the Skull Building. This variation provides an opportunity using isotope methods to test whether there was evidence for structuring of daily activities (diet in this case) that might serve to reinforce this change in burial practice. We show that when the inhabitants of Çayönü Tepesi changed their architecture and operated different burial practices in conjunction, this coincided with other aspects of behaviour including socially-constituted food consumption practices, which served to reinforce social identities.     

Human impact around settlement sites: a phytolith and mineralogical study for assessing site boundaries,phytolith preservation,and implications for spatial reconstructions using plant remains

Defining the extent of human activity around settlement sites is of particular significance in archaeology as it may define peripheral activity areas and thus the site's boundary. In Near Eastern archaeology, site boundaries are usually defined by the presence of architectural and other macroscopic archaeological remains. Here we use the phytolith concentrations and morphotype assemblages, as well as changes in the mineralogical composition of the sediments in and around the small Iron Age site of Izbet Sartah in central Israel to determine the site boundaries. The site has a shallow stratigraphy and highly bioturbated sediments. Coincidental changes in the clay/quartz ratio and phytolith concentrations define the boundary between high and low impact anthropogenic activities. This boundary is generally some 20 m away from the architectural remains. In addition, we note that the phytoliths in the site's core show clear evidence of having been affected by chemical dissolution (i.e., diagenesis), while those in the vicinity of the site's boundary have undergone severe diagenesis. These observations indicate that phytolith diagenesis will affect site boundaries determination, as well as phytolith-based reconstructions of activity areas. We propose that phytolith preservation depends on the initial amount of available silica, the depth of burial with respect to the active root area of modern vegetation, and the presence of fresh phytoliths in the soil.     

Application of magnetic susceptibility on polished stone tools from Western Hungary and the Eastern part of the Czech Republic (Central Europe)

A non-destructive method – magnetic susceptibility (MS, κ) measurement – was used to characterize about 360 Neolithic, Copper and Bronze age polished stone tools of the Miháldy collection (Laczkó Dezső Museum, Veszprém, Hungary), composed of artefacts from three Neolithic (Moravian Painted Ware) workshops around a source of greenschist at Želešice near Brno (Moravia, Czech Republic) and semifinished artefacts from workshops at Neolithic mines in the Jizerské hory Mts. (Bohemia, Czech Republic); in addition, rock reference samples collected from the possible provenance areas and rock outcrops in the Czech Republic, Slovakia and Hungary were analysed as comparative material with the same method. We elaborated a model experience to control the thickness correction factor (TCF) following Williams-Thorpe et al. (2000).Among various stone raw materials of polished artefacts we found basaltic rocks and greenschists-metabasites as the most common.     

Reconstructing late Holocene palaeoenvironments in Bangladesh: phytolith analysis of archaeological soils from Somapura Mahavihara site in the Paharpur area,Badalgacchi Upazila,Naogaon District,Bangladesh

Palaeoenvironmental reconstruction based on phytolith analysis of late Holocene-aged soils in and around the Somapura Mahavihara archaeological monastery site in the Paharpur area, Badalgacchi Upazila of Naogaon District in northwestern Bangladesh was undertaken. Results indicate five climate zones marked by alternatively cool and temperate events. The phytolith assemblages of Zones 1, 3 and 5 are clearly characterized by a higher proportion of Pooideae phytoliths. The climate indices for these zones are all greater than 50, indicating cooler climatic conditions. In contrast, the phytolith assemblages of Zones 2 and 4 are typified by a higher proportion of Panicoideae phytoliths; their climate indices are 42 and 44, respectively, indicating the presence of a warmer, more temperate climate. In general, the dominance of grasses over broad- leaved trees throughout the sequences suggests that generally cool to temperate and dry conditions with some cyclical variability persisted around Paharpur and the surrounding region throughout the Pala Dynasty from AD 730 to 1080. The question of whether these shifts in vegetation might be linked with El Niño Southern Oscillation events, and whether these might in turn have cultural responses, is raised.     

山东日照市两城镇遗址龙山文化先民食谱的稳定同位素分析

山东日照两城镇遗址出土的浮选植物标本和植桂体表明,稻米和粟是该遗址龙山先民重要的食物来源,但由于动物骨骼保存很差,我们对他们的肉食来源所知甚少。而采用碳氮稳定同位素法探讨古人的食性和食物结构,对各种同位素含量不同的食物在当时人们食谱中的相对重要性提供了补充定量的数据。     

Stability of phytoliths in the archaeological record: a dissolution study of modern and fossil phytoliths

Opaline phytoliths are important microfossils used in archaeological and ecological research. Relatively little is known about the stability of phytoliths after burial. Under alkaline pH conditions they can dissolve, and mechanical disturbances can cause a loss of their more delicate appendages. Here we present an experimental study of phytolith stability (combination of solubility and abrasion). Modern and fossil phytoliths were extracted from wheat using new methods to minimize dissolution, and by burning in an oven. These assemblages were placed in a solution buffered to pH 10 and maintained under constant temperature and shaking conditions. The silicon concentrations in the solution were monitored once a week for 5 weeks. The phytolith morphologies in each assemblage were determined at the outset of the experiment and after 5 weeks. The results show that there are differences in stability between various assemblages. Modern inflorescence wheat phytolith assemblages are more unstable than those from leaves/stems. Burnt assemblages are less stable than unburnt assemblages, and a fossil phytolith assemblage about 3000 years old is more stable than the modern wheat assemblages. The results also show that individual phytolith morphotypes have different stabilities, and as a result of dissolution and abrasion, some morphotypes may resemble others. This study further shows that archaeological and/or paleo-environmental interpretation of phytolith assemblages may change with the assemblage’s state of preservation.     

Investigating crop processing using phytolith analysis: the example of rice and millets

The application of crop processing models to macro-botanical assemblages has traditionally been used to interpret past husbandry practices and organisation of labour involved in crop-processing. Phytoliths offer an alternative method of analysis because they are durable in most environments, regardless of whether plant parts are charred, and the identification of plant types and plant parts allows them to be used in much the same way as macro-botanical remains. Indeed macro-remains and phytoliths are complementary datasets for examining the input of plant parts, such as crop-processing waste, into archaeological deposits. We outline crop-processing models in relation to macro-remains and then develop the framework for their application to archaeological phytolith assemblages. Rice and millet processing models are explored in relation to patterns expected in both macro-remains and phytoliths. The utility of these models is demonstrated with archaeological evidence from the site of Mahagara, an early farming site in North-Central India. The results indicate a way to employ phytoliths in archaeology which complements the fragmentary evidence available from plant macroremains.     

One,two, three phytoliths: assessing the minimum phytolith sum for archaeological studies

The number of phytolith studies has increased steadily in the last decades in palaeoecological as well as archaeological research, and phytolith analysis is currently recognised as a proper area of expertise within archaeobotany. This has led towards a strengthening in the standardisation of the different steps involved in analysis; e.g. sampling strategies, laboratory extraction or processing of plant material/soils for the creation of reference collections. In spite of this, counting procedures remain one of the areas that could be further developed. The aim of this paper is to assess representativeness of phytolith count size in archaeological samples and specifically to assess whether an increase in total number of individuals counted influences the number or distribution of morphotypes observed. Two statistical tests are performed to evaluate the representativeness of count size: phytolith sum variability analysis (PSVA) and morphotype accumulation curve (MAC). The analyses show the relationship among the number of counted phytoliths, the variability (that is, the number of different morphotypes identified) and the stabilisations of the MACs. Results allow us to support the standard count size in phytolith studies, which ranges from 250 to 300 particles. Together with a quick scan, this strategy should produce a precise and clear phytolith assemblage for archaeological studies.     

The phytolith archaeological record: strengths and weaknesses evaluated based on a quantitative modern reference collection from Greece

The phytolith record from archaeological strata is a powerful tool for reconstructing aspects of past human behaviour and ecology. Considerable insights into the problems and potentials of this record can be obtained by studying phytoliths in modern plants. We prepared a phytolith reference collection of modern plants mostly from northern Greece that includes quantitative information on phytolith concentrations (number of phytoliths per gram dry organic material) and morphological assemblages. Here we analyse this reference collection with the aim of evaluating the strengths and weaknesses of the archaeological phytolith record. The reference collection comprises 62 different plant taxa. Of them, 28 are trees, 1 conifer, 10 shrubs and 1 herb, 3 domesticated annual legumes and 19 monocotyledons. We demonstrate quantitatively the extent to which woody species, legumes and fruits of dicots contribute only small amounts of phytoliths to the sediments per unit tissue dry weight, while leaves of trees and shrubs produce significant amounts of phytoliths and grasses are prolific phytolith producers. We compare the data of this reference collection with a similar reference collection from another Mediterranean ecosystem (Israel). The comparison indicates that some aspects of phytolith production are probably genetically controlled, whereas others are environmentally controlled. We note that despite the fact that woody species produce few phytoliths per gram of tissue, their record can be most informative when taking into account other properties of the sediments. We also note that jigsaw puzzle-shaped phytoliths normally form in plants that grow in humid conditions, but may form in arid environments when the plants are irrigated. This study clearly shows the extent to which some plants can be under-represented and others over-represented in the phytolith record. Knowing the extent of this bias can greatly improve our interpretation of the phytolith record.     

Ethno-geochemical and Phytolith Studies of Activity Related Patterns: A Case Study from Al Ma’tan,Jordan

ABSTRACT

Understanding Neolithic sites in southwest Asia is often difficult because of the lack of preservation of organic remains and the effects of various taphonomic processes that alter the original record. Here, we use an ethnographic approach to test the potential of using plant phytoliths and geochemistry to aid our interpretation of southwest Asian Neolithic sites. Our study of a recently abandoned stone and mud constructed village in Jordan, shows that for certain activity types, phytoliths and geochemistry can help distinguish different construction methods and functions, particularly for burnt areas, animal use areas and where there has been the addition of a specific construction material. For features constructed from the same source materials distinctions are more problematic. Geochemical and phytolith proxies were individually effective in distinguishing activity areas and construction materials, but signals were diminished when the statistical analysis was run on both forms of evidence combined. It is therefore recommended that the data from plant phytolith and geochemical analyses are subject to separate statistical tests and that the two sets of results are used in combination to interpret archaeological sites and their uses.     

Identifying past agricultural activity through soil phytolith analysis: a case study from the Hawaiian islands

Soil samples from documented archaeological sites and adjacent fields were analyzed for phytolith assemblage in an effort to identify and reconstruct prehistoric and early historic agricultural systems on the Island of Hawaii. The removal of indigenous tree and shrub vegetation, subsequent agricultural activity, and eventual abandonment of the fields can be identified in the areas tested. The applicability of phytolith analysis to archaeological problems is advanced and areas of strength and weakness identified.     

The status of phytolith analysis in the American tropics

Phytolith analysis is now sufficiently mature to provide an independent source of data in tropical paleoethnobotany and paleoecology. At the same time, ongoing studies of phytoliths from tropical plants and sediments are discovering new applications. There are, to be sure, problems in interpreting prehistoric plant use and paleoecology which cannot be addressed through phytolith analysis; some of the more important ones are listed. Through a review of recent studies in the New World tropics, this paper presents some contributions to, problems of, and prospects for phytolith analysis to inform the archaeological community about such issues as (1) the origins and dispersals of domesticated plants, (2) the development of tropical forest agriculture, (3) the uses of tropical plants in prehistory, and (4) the distribution and composition of past plant communities.     

Identification of pastoral sites using stable nitrogen and carbon isotopes from bulk sediment samples: a case study in modern and archaeological pastoral settlements in Kenya

The identification of pastoral sites in the East African archaeological record is problematic. Recently, a method for the identification of degraded livestock enclosure sediments had been developed that takes into account the geoarchaeological indicators of micromorphology, phytolith concentrations and the mineral assemblages. This suite of indicators may not always be present in degraded livestock enclosure sediments. This study presents an additional indicator by which degraded livestock enclosure sediments may be identified, namely the isotopic composition of organic nitrogen measured on bulk sediment samples. We studied a highly controlled ethnoarchaeological sequence of abandoned Maasai livestock enclosure sediments sampled in Rombo area, southern Kenya. The results were compared to archaeological sediments from the Elmenteitan Pastoral Neolithic site of Sugenya, southwestern Kenya, radiocarbon dated to ca. 2000 BP (uncalibrated). The sediments from both sites were studied using all four types of analyses, i.e., micromorphology, mineralogy, phytolith concentrations, and stable carbon and nitrogen isotopic compositions on bulk sediment samples. The results show that in abandoned livestock enclosure sediments of known ages a significant enrichment in the heavy nitrogen isotope (¹⁵N) occurs, and that carbon isotopic compositions may be useful for differentiating cattle from caprine enclosures following their dietary preferences (i.e., grazers vs. browsers). A similar pattern of ¹⁵N enrichment is observed in sediments sampled within the site of Sugenya while sediments sampled outside the site's perimeter are generally depleted in ¹⁵N. The micromorphological, mineralogical and phytolith analyses support the conclusion that the sediments from within the site of Sugenya represent degraded livestock enclosure sediments. The carbon isotopic composition from the degraded dung deposits strongly suggests that livestock kept at Sugenya were cattle. Overall, this study presents new empirical data that can be used for the identification of livestock enclosures, and shows that the isotopic signatures and geoarchaeological indicators can preserve for at least two millennia.     

Geoarchaeology in an urban context: The uses of space in a Phoenician monumental building at Tel Dor (Israel)

Interpretation of past urban societies in the Near East, whose settlements are known mostly as tell sites, is largely based on macro-stratigraphy and on the association of architecture with macroscopic artifacts. Analyses of sediments, common in prehistoric sites, are rare in tell sites. Here we show the results of a detailed geoarchaeological study of the micro-stratigraphy of a sedimentary sequence associated with early Iron Age Phoenician monumental architecture. The study involves mineralogical, micromorphological and phytolith analyses and provides new insights into the stratigraphic sequence and the use of architectural spaces. The sedimentary sequence examined comprises alternating layers of gray ‘fill’ deposits and white ‘floors’. We show that ‘floors’ made from local calcareous sandstone in the lower part of the sedimentary sequence were heated and are thus in effect ‘plaster floors’. A concentration of micro-laminated, trampled fish remains above the most elaborate of these plaster ‘floors’ indicates activities related to fish processing. Fine white layers in the upper part of the sedimentary sequence that were considered as plaster based on macroscopic examination are in fact composed almost entirely of opaline grass phytoliths. The phytoliths appear in an undulating micro-laminated structure and are associated with dung spherulites and phosphate nodules, thus probably reflecting livestock penning. The formation of ‘phytolith floors’ involves extensive volume reduction due to the degradation of the organic material and this may result in ‘floor’ subsidence, a phenomenon that is often observed in archaeological sites. Most ‘fill’ deposits include macroscopic and microscopic remains of wood ash, bones, phytoliths, charcoal, ceramics, plaster and mollusk shells, reflecting the debris produced from household activities. This study shows how a combination of macro-stratigraphy with microscopic and mineralogical analyses of the sediments within architectural spaces can provide information on the varying ways in which the space was used through time, and also contributes to solving macro-stratigraphic problems.     

A Comparative Analysis of Wet and Dry Ashing Techniques for the Extraction of Phytoliths from Plant Material

Two methods are commonly used for the extraction of phytoliths from plant material to be used as reference in the analysis of archaeological phytolith samples: (1) spodograms or dry ashings; and (2) acid digestions or wet ashing. It has been suggested that these techniques may modify the resultant samples in different ways. Dry ashing, in particular, has been implicated as a cause of shrinkage and warping in phytolith assemblages when incineration occurs at ≥450°C. The results of a morphometric comparative analysis between the dry ashing and wet ashing methods do not support these claims. This study establishes that differences in patterns of dimension and curvature of short bilobate phytoliths and of elongate phytoliths both subjected to dry and wet ash preparation are not statistically significant. There is, therefore, no detectable evidence of morphological impact as a result of these methods. This finding implies that any differences that do occur in phytolith size and curvature are typical, possibly random permutation within assemblages, or that they are the result of variation in leaf cell structure rather than the consequence of a particular extraction procedure. This suggests that the practice of using different methods of preparation of reference samples for fossil analysis can be reliably continued.     

Early Middle Ages Houses of Gien (France) from the Inside: Geoarchaeology and Archaeobotany of 9th–11th c. Floors

ABSTRACT

At Gien (France), indoor floors from early Middle Ages occupation (8th–10th c. AD) are very well preserved, providing a new reference for archaeological investigation in northern France. This site is located on an outcrop, 20 m above the Loire valley, where a 15th c. castle stands now. The medieval occupation combines high-status houses with crafting and agricultural areas. They constitute a new urban nucleus, which grew 2?km east from an ancient Roman settlement. During the rescue excavation, four buildings of different status were sampled and studied using an integrated approach, combining stratigraphy, micromorphology, chemical, macro-remain and phytolith analyses. Micromorphological investigations helped to identify 74 built floors, from 0.5 to 150?mm thick, made with transformed local clay or imported silty earth. Mineral floors were covered by vegetal ones, consisting of crop processing refuse. These litters include an abundance of phytoliths and some seeds, both produced by cultivated cereals, which were processed in situ, such as Triticum durum, Secale cereale and Hordeum vulgare. The refuse above the mineral and vegetal floors were trampled. They were produced not only by domestic activities, such as cooking and eating, but also by metallurgic activities and animal husbandry. The investigation of a contemporary pit indicated that, despite the large amount of refuse, floors were well maintained and regularly rebuilt. The spatial distribution of waste indicated that a single space could be dedicated to several activities, which were not necessarily separated by new floors. Moreover, the total absence of bioturbation allowed the study of a stage of dark earth formation, by comparing it to the contemporaneous mechanical disturbance of a part of the strata which occurred when building new floors. All these results give new evidence of the richness and the complexity of the early Middle Ages town, in addition to help identifying the activities which could take place in early castral areas.     

‘True British sailors’: a comment on the origin of the men of the Mary Rose

Bell et al. (2009) have recently published an isotopic investigation of the origins of 18 men whose remains were found in the wreck of the Mary Rose, Henry VIII's warship, which sank in 1545. They conclude that a high proportion of the ship's crew were foreigners and that this may have contributed to confusion on board ship and the sinking of the vessel. We have re-evaluated the data of Bell et al. and conclude that only one of the 18 sailors demonstrably spent his childhood outside the British Isles.