Age estimation of children from prehistoric Southeast Asia: are the dental formation methods used appropriate?

Inter-population differences in skeletal and dental growth and maturation are acknowledged frequently in the biological anthropological literature. These growth differences have implications for the reliability of standards for the estimation of age at death of archaeological subadults. The number of archaeological projects that are recovering human burials from non-European contexts, including Southeast Asia, and the increasing interest in subadult bioarchaeological studies provides the impetus for investigating this issue of ageing subadult individuals from these populations. This paper aims to address some of the problems of the representativeness of ageing standards for non-European children in bioarchaeology. This is achieved through a literature review of the issue of growth variability and age estimation, and a basic comparison of the commonly applied age estimation method based on North American children with a dental formation study of modern Thai children. Although these studies do not employ similar methods the Thai study is the only comparable data available and therefore serves as a starting point to address these issues. The results raise an important question for bioarchaeologists of the appropriateness of available ageing methods. In addition this paper emphasises the need for the use of appropriate methodologies in the collection and presentation of dental formation data.     

Sample-specific (universal) metric approaches for determining the sex of immature human skeletal remains using permanent tooth dimensions

In this paper, three approaches for developing sample-specific sex determination methods of immature skeletal remains based on permanent tooth dimensions are proposed and tested using a sample of identified skeletons. The sample comprises adult and subadult individuals selected from the Lisbon documented skeletal collection, housed at the National Museum of Natural History in Lisbon, Portugal. Faciolingual and mesiodistal diameters were the tooth dimensions utilized. In the first approach, sex-specific logistic regression formulae based on adult tooth dimensions are developed and then used to determine the sex of the subadult sample. The second and third approaches are based on the sectioning point procedure, which uses the overall mean of a measurement (tooth diameter) collected from the sample as the discriminant criteria for determining the sex of the individuals in that sample. While in the second approach the adult overall mean of each dimension is used as the discriminant criteria for determining the sex of the subadults, in the third approach it is the subadult overall mean of each dimension that comprises the discriminant criteria that is applied back to the subadults for determining sex. Results show that the canines are the teeth with the highest sexual dimorphism and methods of sex determination based on canine dimensions provide correct allocation accuracies between 58.8% and 100% depending on the diameter and the approach that is being used. Canine faciolingual dimensions provide the best overall results. Combinations of measurements from the same and different teeth do not increase significantly the accuracy of the methods and approaches. Some of the problems of subadult sex determination methods based on adult tooth dimensions result from differing levels of sexual dimorphism between the adult and subadult segment of the sample. Mortality or cultural bias may increase or decrease the sexual dimorphism of the subadults compared to the adults. Small subadult samples utilized in this study may also raise questions regarding the accuracy of the three different sample-specific approaches. However, high consistency of results using the canine and different approaches, suggests that adult and subadult canine dimensions can be reliable sex discriminators of immature skeletal remains in archaeological samples. The major advantage of the approaches presented here is that they can be used to derive sample-specific methods and, therefore, eliminate the problem of applying morphological or metric methods to individuals originating from a population that differs from the one that contributed to the development of the method.     

Skeletal evidence for morbidity and mortality in Copper Age samples from northeastern Hungary

Analysis of 183 human skeletons representing the Copper Age in northeastern Hungary indicates slightly less morbidity and mortality than found in previous studies of later Bronze Age and Iron Age samples from the same area. Mean adult age at death was 33.4 years for males and 32.9 years for females. Life table reconstruction revealed a life expectancy at birth of about 28 years, and at age 15 of about 17 years. Frequencies of dental hypoplasia (<1%) and carious lesions (2.3%) were relatively low. Comparisons of the Copper Age data reported here with previously published studies of later Bronze Age and Iron Age samples from the same area revealed little or no change in life expectancy at age 15, long bone diaphyseal circumference, estimated living stature, frequencies of dental hypoplasia, alveolar abscesses, tooth loss, adult porotic hyperostosis or trauma. Temporal increases were detected in life expectancy at birth, dental caries frequency, cribra orbitalia, subadult periosteal lesions and vertebral osteoarthritis. The study is part of a larger effort to examine long‐term temporal changes in skeletal samples from that region. Copyright © 2008 John Wiley & Sons, Ltd.     

Consequences of conquest? The analysis and interpretation of subadult trauma at Puruchuco-Huaquerones, Peru

This paper examines bioarchaeological evidence of violence and traumatic injury on subadult skeletal remains from two Late Horizon (A.D. 1470-1540) cemeteries within the archaeological zone of Puruchuco-Huaquerones, Peru. Here we present the frequency and types of traumatic lesions on the 242 subadults analyzed. We observed significant increases in the frequency of subadult trauma, particularly among the burials associated with Spanish Conquest. Specifically, we noted a statistically significant increase in the frequency of cranial trauma in a subsample of individuals from one of two cemeteries at the site, 57AS03. These perimortem cranial injuries suggest an intensification of violence and lethality that may have affected the children from this community. We then discuss the biocultural implications of this analysis within the context of Spanish invasion and conquest.     

Polydactyly in the prehistoric American Southwest

General surveys of skeletal collections from Arizona for evidence of trauma and pathology led to the identification of polydactyly in two subadults. Polydactyly is a congenital condition characterised by the presence of extra digits on the hands or feet. Both affected subadults exhibit a sixth digit in the form of a branching fifth metatarsal. One of the affected individuals is an infant from the Tapia del Cerrito site exhibiting Y‐shaped fifth metatarsals indicative of postaxial type A polydactyly. The second individual is a juvenile from the Nuvakwewtaqa (Chavez Pass Ruin) site exhibiting a left fifth metatarsal with a lateral branch, also diagnosed as postaxial type A polydactyly. These two cases appear to be the first examples of polydactyly from archaeological contexts identified among subadults, and bring the total number of known cases from the American Southwest to six. Discovery of so many examples of this relatively rare condition amongst the Puebloan people of the Southwest adds to the evidence that, rather than simply being an artistic motif, rock art depictions featuring hands and feet with six digits were probably inspired by observation of the condition amongst living people. Furthermore, burial treatment of the Tapia del Cerrito infant suggests that polydactyly may have conferred a special status on the bearer. Copyright © 2006 John Wiley & Sons, Ltd.     

Modeling Breastfeeding and Weaning Practices (BWP) on the Coast of Northern Chile's Atacama Desert During the Formative Period

Stable isotope analysis of bone collagen is frequently employed as a means of studying the breastfeeding and weaning practices (BWP) of archaeological populations. Such studies are strengthened greatly through the application of statistical models that permit precise and model-bound estimates of weaning age, duration, trophic enrichment, and the isotopic characterization of supplementary foods. Here we present the result of a stable isotope (δ¹⁵N) and Bayesian computational modeling study of bone collagen from human subadults from two coastal cemetery sites located near the mouth of the River Loa in the Atacama Desert. Recent bioarchaeological and paleodemographic research on remains from these marine hunter-gatherer sites, which are contemporary with the Formative Period (1500 BC–AD 400), has found evidence for notably elevated rates of female fertility. Ultimately, we argue that the modeled BWP parameters, which indicate the early introduction of supplementary foods, support an argument of high fertility as gleaned from the bioarchaeological evidence, and that these results provide novel insights into the child-rearing practices of the coastal populations of the Atacama. Indeed, these populations would have seemed to have developed a set of BWP that carefully balanced the biological and economic production/reproduction of the community.     

Subadult Age at Death and Health Status at Niah Cave,Borneo (1500–200 bc)

Southeast Asia's transition to rice agriculture is often used as an exception to the general pattern of health decline at the agricultural transition. Niah Cave is the largest known Southeast Asian Neolithic cemetery, providing valuable information about subadult health during the agricultural transition in this region. This study examines the health status and mortuary treatment of subadult skeletal remains (Minimum Number of Individuals (MNI) = 49) from the Neolithic cemetery of Niah Cave, Sarawak, Malaysia (1500–200 bc ). This study found that few cases of cribra orbitalia (4.6%) and porotic hyperostosis (14%). However, nonspecific infections may have been a risk factor for subadults as over half (65%) died with active cases of periosteal reactions on their long bones. Differential diagnosis determined that possible factors for this include parasite load, rickets and scurvy. Mortuary practices indicate that subadults may have been treated differently in phases 1 and 2 but were grated similar mortuary treatment in phases 3 and 4. This study suggests that changes in mobility patterns may have placed subadults at risk for increased parasite load and vitamin deficiency, while the role of subadults within the community may have changed over time. Copyright © 2017 John Wiley & Sons, Ltd.     

Panel regression formulas for estimating stature and body mass from immature human skeletons: a statistical approach without reference to specific age estimates

Anthropologists require methods for accurately estimating stature and body mass from the human skeleton. Age-structured, generalized Least Squares (LS) regression formulas have been developed to predict stature from femoral length and to predict body mass in immature human remains using the width of the distal metaphysis, midshaft femoral geometry (J), and femoral head diameter. This paper tests the hypothesis that panel regression is an appropriate statistical method for regression modeling of longitudinal growth data, with longitudinal and cross-sectional effects on variance. Reference data were derived from the Denver Growth Study; panel regression was used to create one formula for estimating stature (for individuals 0.5–11.5 years old); two formulas for estimating body mass from the femur in infants and children (0.5–12.5 years old); and one formula for estimating body mass from the femoral head in older subadults (7–17.5 years old). The formulas were applied to an independent target sample of cadavers from Franklin County, Ohio and a large sample of immature individuals from diverse global populations. Results indicate panel regression formulas accurately estimate stature and body mass in immature skeletons, without reference to an independent estimate for age at death. Thus, using panel regression formulas to estimate stature and body mass in forensic and archaeological specimens may reduce second stage errors associated with inaccurate age estimates.     

The Bioarchaeological Investigation of Childhood and Social Age: Problems and Prospects

Recently, the value of the study of children and childhood from archaeological contexts has become more recognized. Childhood is both a biological and a social phenomenon. However, because of specialization in research fields within anthropology, subadults from the archaeological record are usually studied from the biological perspective (bioarchaeology) or, more predominantly, the social perspective (social archaeology), with little research that incorporates both approaches. These polarized approaches to childhood and age highlight the dualistic way in which “biological” and “social” aspects of the body are viewed. Some recent literature criticizes bioarchaeological approaches, and calls for the incorporation of childhood social theory, including social age categories, into subadult health analysis. However, few studies have explicitly addressed the practicalities or theoretical issues that need to be considered when attempting this. This paper critically examines these issues, including terminology used for defining subadulthood and age divisions within it, and approaches to identify “social age” in past populations. The important contribution that bioarchaeology can make to the study of social aspects of childhood is outlined. Recent theoretical approaches for understanding the body offer exciting opportunities to incorporate skeletal remains into research, and develop a more biologically and socially integrated understanding of childhood and age.

Cribra orbitalia,sinusitis and linear enamel hypoplasia in Swedish Roman Iron Age adults and subadults

Maxillary and frontal sinusitis, cribra orbitalia and linear enamel hypoplasia were recorded as indicators of nutritional and environmentally related stress in an Early Iron Age (0–260 AD ) population from Sweden. The material comprised the skeletal remains of 99 adults and 45 subadults (aged 20 years and under). Maxillary sinusitis and cribra orbitalia were recorded in the majority of subadults and adults. In contrast, linear enamel hypoplasia was uncommon and occurred mainly in the subadults. In seven cases (12.7%) there was a clear co‐occurrence of periapical lesions and maxillary sinusitis. A significant co‐occurrence of maxillary sinusitis and frontal sinusitis was found in the adults. The least common combination in sex‐determined adults was cribra orbitalia and enamel hypoplasia, only 7.7% exhibiting both lesions. The significantly higher incidence of this combination among subadults suggests an association with life‐threatening conditions. Overall, the results showed that the Early Iron Age community studied suffered foremost from upper respiratory diseases. Copyright © 2010 John Wiley & Sons, Ltd.     

Did Children in Medieval and Post‐medieval Poland Suffer from Scurvy? Examination of the Skeletal Evidence

Late‐medieval and post‐medieval writings report that scurvy was a widespread condition in medieval and early historical Poland. Archaeological and historical data indicate that the diet of children was based on foods poor in vitamin C and contained small amounts of raw plant products. Also, historians emphasise that in medieval and post‐medieval Poland, there were seasonal fluctuations in food availability, frequently accompanied by poor harvests. Both resulted in long periods of poor nutrition, which affected children most severely. The aim of this study was to investigate skeletal manifestations of scurvy in subadult remains from medieval and post‐medieval Poland. Following standards described by Ortner and colleagues, anatomical sites pathognomonic of scurvy in subadults (<17 years) were assessed for abnormal porosity and hypertrophic bone among skeletons excavated from three sites: Ostrów Lednicki (dated to the 11th–14th centuries AD), Cedynia (10th–14th centuries AD) and Słaboszewo (14th–17th centuries AD). In total, 3.6% of all examined children were found to bear traces of vitamin C deficiency. The prevalence of scorbutic lesions was 4.5% for Cedynia, 2.6% for Ostrów Lednicki and 3.6% for Słaboszewo. The majority of affected children were less than 7 years of age. Scurvy was likely more widespread in the living populations than it appears from the calculation of skeletal markers, because some individuals might have recovered or died before obvious traces became apparent. Also, in some children, scurvy might not have reached an advanced stage, identifiable in the skeletal material. The prevalence of scurvy reflects not only dietary patterns but also food storage and preparation techniques adopted in the Polish territories during the Middle Ages, which contributed to low intakes of vitamin C. Copyright © 2015 John Wiley & Sons, Ltd.     

Estimating age by tooth wear of prehistoric human remains in Brazilian archaeological sites

The occlusal surfaces of 298 permanent maxillary and mandibular molar teeth of prehistoric shellfish‐gatherer subjects from the Piaçaguera and Tenorio sites (4930 to 1875 BP), near the central‐northern coastline of São Paulo, Brazil, were examined for classification of macro‐wear stages. Molar tooth wear is an indication of masticatory activity and can be used in the estimation of age at death. The examination of visual and schematic aspects of occlusal macro‐wear used a visual chart proposed by Brothwell, which includes the three superior and inferior, left and right, permanent molars. Three examiners performed the macroscopic observations twice under the same conditions. The resulting age estimates were compared with previous information of age estimated by skeletal examination. A reduced intra‐ and inter‐observer variation was observed; all re‐examinations indicated discrepancies of less than two years for the upper and lower limit of the age range estimates. The procedure was also considered consistent with the skeletal method used for age estimation of human remains excavated in Brazilian archaeological shell mounds, with a discrepancy of less than 8.22 years between the upper and lower limits of estimates by both methods. Age classification by the occlusal molar wear may be a useful tool for the classification of archaeological findings, mainly when only fragmentary skeletal remains are excavated. The current results indicate that the application of the Brothwell chart for Brazilian archaeological series presented satisfactory results of consistency, and its expanded use may represent a relevant adjunct for research. Copyright © 2006 John Wiley & Sons, Ltd.     

A regional investigation of subadult dietary patterns and health in late Iron Age and Roman Dorset,England

This is the first regional analysis of the impact of Romanisation on subadult dietary patterns and related health parameters in Britain. A sample of 200 subadults from late Iron Age (LIA) and Romano-British (RB) Dorset were examined for dental health and specific metabolic diseases, and a sub-sample of 29 individuals were selected for nitrogen and carbon isotope analysis. The results showed that dental health declined in the Romano-British period and the incidence of scurvy and rickets rose. Increased consumption of marine foods in the RB period is indicated by an increase in δ¹³C between the LIA and RB subadults. After early childhood, there was no age-dependent variation in dietary protein in the RB and LIA populations from Dorset. We propose that these changes related to the introduction of urban living, Romanised diets and population migration.     

Population-specific stature reconstruction for medieval Trondheim,Norway

Temporal changes in average stature are often used as a measure of a past population's adaptation, or lack of it. Traditionally, stature estimates have been calculated using formulae derived from limb proportions of cadavers. However, many authors have noted the problem of regional or population variation in body proportions of such reconstructed ratios. Before differences in stature can be attributed to environmental adaptation, ‘ethnic’ or population differences in limb ratios must be taken into account. The present paper calculates the stature of a medieval Norwegian skeletal sample using archaeological plan femur length and dry bone femur length. The author presents a variety of formulae and compares the stature derived from these calculations to the stature derived from archaeological plans. The Trondheim statures are then compared to stature reconstructions of other contemporary populations.     

New Statistical Approaches to Intra‐individual Isotopic Analysis and Modelling of Birth Seasonality in Studies of Herd Animals

This paper introduces improved methods for statistically assessing birth seasonality and intra‐annual variation in δ¹⁸O from faunal tooth enamel. The first method estimates input parameters for use with a previously developed parametric approach by C. Tornero et al. The second method uses a non‐parametric clustering procedure to group individuals with similar time‐series data and estimate birth seasonality. This method was successful in analysing data from a modern sample with known season of birth, as well as two heterogeneous archaeological data sets. Modelling indicates that the non‐parametric approach estimates birth seasonality more successfully than the parametric method when less of the tooth row is preserved. The new approach offers a high level of statistical rigour and flexibility in dealing with the time‐series data produced through intra‐individual sampling in isotopic analysis.     

Schmorl's nodes: clinical significance and implications for the bioarchaeological record

Back pain is one of the major contributors to disability and loss of productivity in modern populations. However, osteological correlates of back pain are often absent or, as yet, unidentified. As bioarchaeologists depend on osteological evidence to interpret quality of life in the past, back pain, with its profound effects on modern populations, is largely overlooked in archaeological samples. This study addresses this shortcoming in bioarchaeological analysis by exploring the relationship between a defined vertebral osteological lesion, the Schmorl's node, and its effect on quality of life in a clinical population. Using patient insight, healthcare practitioner diagnoses and MR imaging analyses, this study investigates: (1) Schmorl's nodes and sociodemographic factors; (2) the number, location and quantitative aspects (e.g. length, depth, area) of Schmorl's nodes, and how these influence the reporting of pain; (3) the dynamic effects of Schmorl's nodes, in combination with other variables, in the reporting of pain; and (4) the perception and impact of pain that patients attribute to Schmorl's nodes with regard to quality‐of‐life issues. The results of this study indicate that Schmorl's nodes located in the central portion of the vertebral body are significantly associated with patient reporting of pain, and that the presence of osteophytes, in the affected vertebral region, may increase the likelihood that an individual will report pain. This finding provides bioarchaeologists with an osteological correlate to begin interpreting the presence and impact of pain in archaeological populations, with implications for scoring Schmorl's nodes. Copyright © 2007 John Wiley & Sons, Ltd.     

A new approach to sexual diagnosis in past populations. Practical adjustments from Van Vark's procedure

Determination of sex is a key aspect in the study of past populations. Bias in skeletal sexing is well known, however, and depends upon the completeness of the skeletal remains and the representativeness of reference samples. In order to correct these difficulties, the authors propose practical adjustments to Van Vark's well‐known procedure. Two fundamental aspects are considered to reduce the bias of sex allocation. First, we propose to increase the effectiveness of sexing during the first step (primary diagnosis only from the pelvic bone). Second, to improve the final step (secondary diagnosis from the extra‐pelvic skeleton), we consider posterior probabilities as essential. These two practical adjustments are successfully tested in a sample of known sex and lead to a classification with 94.5% accuracy. These results significantly improve sex determination in archaeological samples and therefore contribute to a better understanding of past populations. Copyright © 1999 John Wiley & Sons, Ltd.     

Investigating foetal and perinatal mortality in prehistoric skeletal samples: a case study from a 3000-year-old Pacific Island cemetery site

The Teouma skeletal sample from Vanuatu represents one of the few truly colonising populations in the Pacific Islands. Therefore, investigating the factors that may have affected foetal/infant mortality in this population is potentially important for understanding the success of settlement in this region of the world. We investigate whether stable isotope analyses of carbon and nitrogen in conjunction with skeletal ageing techniques, can aid in identifying whether the subadults from Teouma died before or after birth in an attempt to understand the potential threats to foetal and infant survival. Multiple skeletal ageing methods using diaphyseal lengths were used to age the young subadults (n = 7). Using regression-based skeletal ageing methods, four of the individuals were aged at around full-term gestation (37–42 weeks gestation), while the remaining three individuals died preterm. The isotope analyses did not assist in identifying the individuals that survived post-birth because none of the subadults displayed the 2–3‰ trophic increase in δ¹⁵N values expected for a breastfed infant, probably as a result of their young age. However, all of the foetal/perinatal individuals exhibited higher δ¹⁵N values in their bone collagen compared with the adult females of the sample, with two of the individuals demonstrating unusually high δ¹⁵N values. The δ¹³C values of the foetuses/perinates did not exhibit the same variation. We explore a number of possible explanations for this elevation of perinatal/foetal δ¹⁵N values and tentatively suggest that this is a result of in utero stress as a consequence of chronic maternal ill-health. The osteological and palaeodemographic evidence supports the assertion that females, foetuses and perinates were susceptible to environmental stress within this colonising population, resulting in early death of the perinatal individuals in addition to early terminations of pregnancy or premature birth possibly caused by infectious and/or metabolic diseases.     

On the accuracy of estimating living stature from skeletal length in the grave and by linear regression

This study evaluates a method for obtaining stature estimates for populations represented by skeletal material, with individuals buried in a supine position. During the excavation of a Danish mediaeval cemetery, in situ skeletal length in the grave was measured from a point above the cranial point farthest from the body to the most distal point of the talus. The measurement was made with a folding rule placed on the sagittal midline of the skeleton, allowed to follow any curvature of the skeleton in situ. In the laboratory, stature was reconstructed anatomically, and this stature was regarded as an accurate estimate of living stature. Stature was also reconstructed from femur length by two linear regression procedures: 1) by sample and sex specific formulae, employing a leave‐one‐out approach, and 2) by sex wise formulae for Euro‐Americans from Trotter & Gleser (1952, American Journal of Physical Anthropology 10 : 463–514). Skeletal length in the grave and the two stature estimates based on linear regression were compared to anatomically reconstructed stature. Skeletal length in the grave estimated anatomically reconstructed stature with practically no bias (95% CI: −1.3–1.5 cm). Sample specific regression formulae estimated anatomically reconstructed stature also with no bias (95% CI: −1.2–1.1 cm). In contrast, statures calculated from Trotter & Gleser's regression formulae estimated anatomically reconstructed stature with a bias of about 4 cm (95% CI: 3.3–5.0 cm). Estimates of stature variance were biased for all three estimation procedures. However, for samples of adults, an adjusted variance estimate can be obtained by subtracting 8.7 cm² from the variance obtained from skeletal lengths in the grave. It is recommended to measure skeletal length in the grave whenever possible, and use this measurement for estimating statures for prehistoric and early historic populations. Copyright © 2005 John Wiley & Sons, Ltd.     

Stable Isotope Analysis of Human and Animal Remains at the Qijiaping Site in Middle Gansu,China

Intercontinental exchanges between communities living in different parts of Eurasia during the late prehistoric period have become increasingly popular as a topic of archaeological research. The Qijia culture, found in northwest China, is one of the key archaeological cultures that can shed light on trans‐Eurasian exchange because a variety of imports are found in this cultural context. These imports include new cereals and animals, which suggest that human diets may also have changed compared with previous periods. To understand human and animal diets of the Qijia culture, carbon and nitrogen isotope ratios from human and animal skeletal remains were analysed from the type site of the Qijia culture at Qijiaping. The results demonstrate that human diet at the site mainly consisted of millet and animals fed on millet. C₃ cereals, such as wheat and barley, did not contribute significantly to human diet, and no isotopic differences were found between adult and subadult diets. Furthermore, three outlying human results raise the possibility of exogenous individuals, perhaps in relation to the parallel movement of animals, crops and goods. This study provides human and animal dietary information for evaluating the nature of exchange and diffusion in eastern Eurasia at this time. Copyright © 2013 John Wiley & Sons, Ltd.