Ages of epiphyseal closure in feral and domestic goats and ages of dental eruption

The bones of 53 goats of known age, sex, and breed were examined to determine the ages of epiphyseal closure, and the mandibles of 36 of them were examined for ages of dental eruption. The ages of epiphyseal closure varied greatly according to the sex and breed of the animal, feral goats being about a year behind domestic goats, and castrate males being anything up to 4 years behind females. In all goats, maturation is much later than in the modern sheep. Ages of dental eruption agreed with earlier findings on the live animal and showed no similar discrepancies.     

舞阳贾湖墓葬的统计学初步分析

本工作旨在了解贾湖单人墓随葬品数量与墓主人性别、年龄以及墓葬分期之间的关系。根据墓葬样本的实际情况,本工作使用t-检验、单因素和双因素方差分析的方法进行了初步的统计学分析。结果显示,贾湖墓葬随葬品总量与性别无关,但与墓主人的死亡年龄有关,壮年随葬品较丰富。同时,随葬品数量受到性别和死亡年龄的交互影响比较显著,女性少年儿童的随葬品数量最多,男性壮年的随葬品次之。从随葬品的种类上看,除少年儿童组最多外,女性壮年墓葬占有石制品最多;男性壮年墓葬占有骨、牙制品最多。本工作还发现陶器和骨、牙制品的数量在不同时期存在着较显著变化,第二期时骨、牙制品和陶制品的数量较多。本工作认为,女孩随葬品数量较多的情况可能与母系氏族社会重女习俗有关,不同性别的壮年侧重随葬不同质地的物品则可能暗示着贾湖先民们已经出现了一定程度的社会分工,陶和骨、牙制品在第二期时比较丰富的现象可能与这一时期渔猎采集活动较活跃有关。     

Interpreting Oral Pathology at Machu Picchu,Peru

This study analyzes oral pathological conditions—caries, antemortem tooth loss, and occlusal macrowear—among adults (N = 70) interred at Machu Picchu, Peru. Previous isotopic diet reconstructions at Machu Picchu suggest substantial early‐life variation that may have narrowed somewhat by the last decade of life. This study seeks to further elucidate the intersection of diet, sex, and health at Machu Picchu by analyzing oral pathological conditions with existing carbon and nitrogen isotopic data. Observed caries prevalence is corrected to control for age and tooth loss, and is described for both anterior and posterior teeth; wear data are scored and reported for individual tooth types. Results indicate caries prevalence consistent with carbohydrate‐rich diets and no significant difference between males and females. However, no significant isotopic parameters are associated with caries prevalence in young adult males, while caries prevalence is significantly associated only with enamel carbonate δ¹³C in older adult males. Dietary protein sources, but not C₄ resources, are associated with caries prevalence in young adult females, and to a lesser degree in older adult females. Significant associations between occlusal wear and caries exist among young adult males and young adult females, but involving different tooth types. These findings suggest differential protein consumption at Machu Picchu between males and females, and separate masticatory and physiological processes shaping caries prevalence between the sexes at the site. This study therefore underscores the role that gender roles may have played in diet variation among this population and the need to account for sex in analyzing oral pathology. Copyright © 2013 John Wiley & Sons, Ltd.     

The chronology of epiphyseal union in the hand and foot from dry bone observations

Dry bone data for epiphyseal union at the hand and foot are scarce, incomplete or inexistent. This study documents the timing of fusion of secondary ossification centres at the hand and foot in a sample of known‐age human skeletons. The sample comprises 92 individuals (49 females and 43 males), between the ages of 9 and 22 years, from the identified skeletal collection curated at the National Museum of Natural History in Lisbon, Portugal. Epiphyseal union was recorded on the right and left side at the long bones of the hand (metacarpus and phalanges) and foot (metatarsus and phalanges), and also at the talus and calcaneus, using a three stage scheme (non‐fused; partially fused and completely fused). In the hand, intra‐observer agreement was 99% and inter‐observer agreement was 98%. In the foot, both intra‐ and inter‐observer agreement reached 100%. Lateral asymmetry was not significant and only 1.1% of the individuals in the sample were found to be asymmetric in the stage of epiphyseal fusion (1.8% in the hand and 0.3% in the foot). A minimum mean advancement of 1–2 years in females relative to males was observed. In the hand bones, epiphyseal union occurred between 12–18 years in females and 16–18 years in males. Comparatively, in the foot bones, epiphyseal union occurred slightly later, with the exception of the talus and calcaneus, which are the earliest bones to mature. Data in this study can be used to estimate the age of unidentified skeletal remains, either directly or by aiding in the modification of incomplete or imprecise data that have been collected over the years. Copyright © 2009 John Wiley & Sons, Ltd.     

Dental pathology and diet at Apollonia,a Greek colony on the Black Sea

Dental pathology has the potential to provide insight into the composition of the diet and to reveal dietary differences based on age, sex and social status. Human skeletal remains from the Greek colonial site of Apollonia (5^th to 2^nd centuries BC) on the Black Sea coast of Bulgaria were analysed for various forms of dental pathology in order to: assess the prevalence of dental disease in the population; compare the dental pathology data from Apollonia with dietary data derived from ancient literary texts and from previous stable isotopic analysis of the colonists' remains; explore variations in dental disease with respect to age and sex; and compare the prevalence of dental pathology in the Apollonians with that of other Greek populations. The composition of the diet, as indicated by the dental pathology data, is consistent with the stable isotopic evidence from Apollonia and with the ancient literary texts, both of which indicate the consumption of a relatively soft, high carbohydrate diet. The higher frequency of dental caries, abscesses, calculus, and antemortem tooth loss in older adults compared with younger ones reflects the age‐progressive nature of these conditions. The lack of significant sex differences in caries, abscesses, calculus and tooth loss corresponds with the stable carbon and nitrogen isotopic data derived from bone collagen, which indicate no significant sex differences in the consumption of dietary protein. In contrast, these findings conflict with the ancient literary texts, which refer to distinct dietary differences between males and females, and with the stable carbon isotopic values derived from bone carbonate, which indicate sex differences with respect to the overall diet. Despite the lack of marked sex differences in dental pathology, overall trends point to subtle dietary differences between males and females. A greater degree of tooth wear in males also hints at possible sex differences in the use of the teeth as tools. Copyright © 2007 John Wiley & Sons, Ltd.     

Age attribution in domestic sheep by skeletal and dental maturation: A pilot study of available sources

The aim of the study was to assess the quality of the available evidence pertaining to the effects of sex and castration on the timing of skeletal and dental maturation in sheep. The initial premise was that our present knowldge of the effects of these factors is inadequate, and that currently available published sources and reference specimens are insufficient to allow confident interpretation of archaeological data. A literature survey was carried out, and adequately documented sheep skeletons in a number of major collections were recorded. The results show that the published evidence is contradictory and ambiguous, particularly with regard to the effects of castration, and that currently available reference material is mostly too diverse in origin and in quality of documentation to allow comparison of well-controlled samples. Some evidence was found to suggest that castration may delay epiphysial fusion during the second year, without delaying fusion of earlier- and later-fusing elements. Overall, it is concluded that much of the received wisdom on skeletal maturation in sheep is poorly founded and unreliable, and that tightly controlled test populations are needed if better data are to be obtained.     

Retracted: A Possible Case of Hypopituitarism in Neolithic China

A human skeleton with a possible case of hypopituitarism is reported. The individual (burial M53) is from the site of Guanjia, a Neolithic settlement in northern China, dated to the Late Yangshao period (6000–5500 bp ). On the basis of the fully erupted third permanent molars and moderate occlusal dental wear resulting in substantial exposure of dentine, the initially estimated age‐at‐death was placed between 26 and 33 years. However, dimensions of the postcranial skeleton fall significantly below and outside the range from contemporaneous adult populations, and along with delayed epiphyseal fusion present throughout the skeleton, the postcranial age is concordant to that of an 11‐ to 13‐year‐old child. Most long bone epiphyses display incomplete fusion or are entirely unfused, but a lack of microporosity in the metaphyseal areas near growth plates indicates a cessation of longitudinal bone growth. Because no signs of porotic hyperostosis, cribra orbitalia, periosteal lesions or linear enamel hypoplasia are observed, the restricted growth of this individual is likely caused by a growth hormone disorder and is unrelated to nutritional deficiencies or systemic infection. Copyright © 2011 John Wiley & Sons, Ltd.     

Αge‐associated Variation and Sexual Dimorphism in Adult Cranial Morphology: Implications in Anthropological Studies

The current paper explores the impact of age and sex on adult cranial shape and size variation in the documented collections from St Bride's and Spitalfields. The scope is to assess the extent to which it is valid to pool age and sex groups in inter‐population comparisons that use cranial data. For this purpose, age and sex differences in cranial shape and size were explored using multivariate analysis of variance, Discriminant analysis and Mann–Whitney tests. The results suggest a clear change in cranial shape with increasing age; however, this change is not statistically significant. Therefore, it is justifiable to pool different age groups in bioarchaeological analyses. Increasing age also has a minimal impact on cranial size among females, whereas among males, its impact is small but statistically significant. Finally, cranial shape and size are significantly different between males and females, irrespective of their age. This dimorphism can be used for the assessment of sex, although attention should be given to over‐classification problems when using discriminant analysis. Copyright © 2012 John Wiley & Sons, Ltd.     

Patterns of dental wear in the early Maori dentition

The levels of tooth wear were surveyed in 50 pre‐contact Maori skulls (23 of them female) from New Zealand. In addition to a generalized tooth wear index, we evaluated occlusal wear for erosion and abrasion. Occlusal microwear was also investigated using scanning electron microscopy (SEM) images and, finally, the occlusal slope was determined at the level of the first molar teeth. The general pattern of wear was one of severe occlusal reduction with no significant differences between males and females. Both silicone impressions and SEM views confirmed a large component (30%) of erosion. While most individuals had flat wear planes, 33% of males and 44% of females showed reversed Monson's curves. Accentuated Monson's curves were found in 23.8% of males. While these results confirm early studies of the excessive wear experienced by pre‐contact Maori, our study shows for the first time that erosion played a significant role in the dental wear of these people. Copyright © 2001 John Wiley & Sons, Ltd.     

Biomolecular study of the human remains from tomb 5859 in the Etruscan necropolis of Monterozzi,Tarquinia (Viterbo,Italy)

Archaeological excavation in an Etruscan room tomb, from the Monterozzi necropolis in Tarquinia led to the recovery of four individuals. It was hypothesized that they could be members of a single family group. As both archaeological data and classical anthropological analysis provided little information in this direction, ancient DNA (aDNA) was extracted from bone and tooth fragments of the individuals. For each subject HVR-I of the mitochondrial DNA (mtDNA) was cloned and sequenced. To identify the sex of the individuals, amelogenine and SRY genes were analysed. Short tandem repeat (STR) characterization was also performed. DNA studies were preceded by the evaluation of amino acids racemization extent and thermogravimetric analysis (TGA), to evaluate, respectively, degradation and quantity of organic matter preserved in the samples.Results show that two subjects are males, whereas two are females. Furthermore, three of them share the same mtDNA sequence, and, as such, they could be related by maternal lineage. This evidence supports the hypothesis that the occupants of the tomb can be considered members of a family group composing two parents and their son and daughter. Molecular study supplies new data to better define the reconstruction previously proposed, based only on a morphological and archaeological approach. Multidisciplinary investigation also allows comparison of the different methods and integration of their contributions.     

A radiographic study of the extent of variation in cortical bone thickness in soay sheep

In order to assess the effect of nutrition on bone mass, radiographs of metopodia from skeletal collections of Soay sheep (Ovis aries) were taken of animals subject to seasonal stress (from the island of Hirta, Hebrides) and those living in good nutritional conditions (from the Institute of Animal Physiology, Babraham, Cambridge). A third set of radiographs was taken at 3 month intervals over a single year on living Soay sheep kept under good conditions at the Rowett Research Institute, Aberdeen.Measurements were taken on the radiographs of length, minimum shaft width (SW) and medullary cavity width (MCW), while combined cortical thickness (CCT) was calculated. The ewes from Hirta had significantly lower values for CCT than those from Babraham, whereas the rams showed no significant reduction in CCT. Since no seasonal variation in CCT was detected in the well nourished Rowett ewes, this indicates a differential effect of poor environmental conditions on bone metabolism in ewes. It is proposed here that this may be related to the additional stress imposed by gestation and lactation on female animals living under conditions of seasonally restricted food supply.     

Spondylolysis in a pre‐contact San Francisco Bay population: behavioural and anatomical sex differences

Spondylolysis refers to a separation of the spinal body from the arch. Researchers have documented that these fractures occur due to stresses related to activities involving the lower limb and back. Spondylolysis in sacral and lumbar vertebrae of 146 (66 males, 66 females, 14 indeterminates) California Amerinds were examined to determine whether sex differences were present. Sacral anatomy (i.e. sacralisation and lumbarisation, sacral base angles, and superior facet morphology) was analysed in relation to spondylolysis and sex, to explore whether sex differences could be better explained through activity patterns or anatomical variation. Spondylolysis afflicted 24 individuals (16.4%). Males had more than twice the rate of spondylolysis than did females (26% and 11%, respectively). Activity patterns, such as thrusting and throwing shafted obsidian points, could explain the sex differences. Males were most frequently buried with obsidian point artefacts, whereas females were buried with mortar and pestles. For sacral anatomy, only males had lumbarisation, and all other anatomical variation had no significant sex differences. Lumbarisation related to spondylolysis in males. In this study, sacral anatomical variation could not fully account for sex differences in spondylolysis; activity patterns provided a better explanation. Nonetheless, anatomical variation may predispose males to spondylolysis, or spondylolysis may affect sacral anatomy. Copyright © 2008 John Wiley & Sons, Ltd.     

Reindeer antlers and what they can tell us about the reindeer population

The form and size of reindeer antlers are due to the animals’ age, genetic constitution, and nutritional condition.

The antlers are shed once a year at different seasons depending on the animals’ age, sex, and, for females, whether they are pregnant or not.

Finds of subfossil antler‐fragments from Heisantj?nn and Sumtangen on Hardangervidda show that the hunting has taken place early in the autumn and that is has been selective for the males.

This article is based on studies of the reindeer, Rangifier tarandus L. on Hardangervidda today, and on a subfossil bone material.     

Raw Material Availability and Technological Choice: Modified Metapodia from an Early Bronze Age Site in Central Israel

This paper explores the incongruous relationship between bone raw material availability and technological choice at the Early Bronze Age site of Horvat 'Illin Tahtit (HIT) in central Israel. At HIT, the worked bone assemblage is mostly comprised of minimally modified sheep and goat metapodia whose function(s) are enigmatic. Most specimens have longitudinally oriented scraping abrasions on at least one face, and of these, some have bilaterally symmetrical drilled perforations on the distal–posterior face. Furthermore, the distal epiphyseal plates of almost all worked metapodia are unfused, whereas unfused epiphyses are nearly absent from the general faunal assemblage. Worked goat metacarpals are significantly more common than sheep metacarpals or metatarsals of either species, despite the greater abundance of sheep over goat in the general faunal assemblage. This paper proposes that the desire to express specific visible attributes of the bone raw material was the key motivation in choosing unfused goat metacarpals over similar, more abundant choices. The analysis considers material properties such as bone mineral density and natural morphology and concludes that material properties between various choices were equivocal. This result suggests that social meanings attributed to specific animals and specific skeletal elements structured bone raw material procurement decisions. Copyright © 2011 John Wiley & Sons, Ltd.     

Tending and drive hunting: A density-mediated attrition model can explain age profiles of white-tailed deer at Iroquoian village sites

In a 1993 paper, Noble and Crerar suggested, based on the age and sex distribution of white-tailed deer (Odocoileus virginianus) longbones from four Attawandaron Iroquoian sites around the western end of Lake Ontario, that the contact-period Attawandaron were managing local deer populations, in response to the aboriginal trade in hides. Having observed similar patterns of differential preservation of elements and epiphyseal fusion at nearby Iroquoian sites that are not ascribed to the Attawandaron, and which pre-date the hypothesised trading period, we argue that these patterns may instead relate to bone density and/or vulnerability to taphonomic effects of late-fusing epiphyses. We further support our argument through age profiles based on dental eruption and wear.     

Fusion status of the jugular growth plate: An aid for age at death determination

To assess the feasibility of the fusion (synostosis) status of the jugular growth plate as an age at death indicator, a series of 98 skulls of documented age and sex from the so-called ‘Utrecht collection’ was examined. Ages at death in the series ranged from 14 to 92 years. Only specimens that could be inspected bilaterally were taken into account. According to the few but conflicting data from the literature, fusion happens at 16–18 years, or starts at ca. 25 years of age. In our sample no fusions were seen before the age of 22 years. Unilateral fusions were found only in the age range 22–34 years. At ages above 34 years in females and above 36 years in males all jugular growth plates were fused bilaterally. These results indicate that examination of the fusion status of the jugular growth plate is of use in age at death determination.     

SEX IDENTIFICATION OF SLAVE SACRIFICE VICTIMS FROM QIN STATE TOMBS IN THE SPRING AND AUTUMN PERIOD OF CHINA USING ANCIENT DNA

Ancient DNA (aDNA) analysis was employed to determine the sex of slave sacrifice victims from Qin State tombs during the Spring and Autumn Period of China. It is difficult to obtain sex information from fragmentary skeleton samples with the aid of skeletal morphology methods. aDNA was extracted from the dentine in selected tooth samples of sacrificial slaves using a modified traditional method, which combines together phenol/chloroform extraction, silicon dioxide adsorption and ultrafiltration concentration. Based on the sequence differences between the amelogenin homologous gene in the X and that in the Y chromosome, a pair of specific primers was designed to identify the sex of the selected samples. In the selected eight typical samples, the aDNA analytical results revealed that three were males and two were females. These findings indicate that molecular sex identification might provide more valuable information for archaeological research on the institution of slave sacrifice in the Spring and Autumn Period of China.     

The frequency of os acromiale in the Robert J. Terry Collection

Normal fusion of the acromion process in humans begins at about age 15 and should complete ossification by age 22–25. Os acromiale occurs when fusion of the acromial epiphysis does not follow its normal ossification pattern, resulting in the retention of a separate epiphyseal end to the acromial process. Depending on how the metaphyseal junctures fuse, differential size and shape of the unattached acromial segment is produced. As a result of shoulder anatomy and the mobile epiphysis, os acromiale is associated with shoulder impingement and rotator cuff problems. The purpose of this study is to document the frequency of os acromiale in the Robert J. Terry Collection and gain a perspective on the occurrence of os acromiale in this specific US population. The overall frequency of os acromiale in the sample of 1594 skeletons was 8.34%. This study also evaluates the prevalence of os acromiale between sexes and ancestral groups in the Terry Collection. Significant frequency differences in the expression of os acromiale were found between both ancestry and sex; the black male sample has the highest frequency (12.47%), with a lower occurrence in the black female sample (9.22%). The white sample exhibited observably lower frequencies, with the white males at 6.8%, and white females at 3.2%. Size and shape and side expression (laterality) showed no significant differences between the sexes. However, significant differences in laterality by ancestry were observed; black males exhibited 48.3% bilateral expression, while white males exhibited bilateral expression only 29% of the time and 54.8% had a right lateral expression. Based on the results presented in this study, it is suggested that there is a genetic causation for os acromiale, which may or may not be exacerbated by physical activity. The use of this anomaly as an additional non‐metric feature in inferring familial relationships and personal identification is discussed. Copyright © 2006 John Wiley & Sons, Ltd.     

Locating Land Use at Neolithic Çatalhöyük,Turkey: The Implications of 87Sr/86Sr Signatures in Plants and Sheep Tooth Sequences

We evaluate local versus distant land‐use models at Neolithic Çatalhöyük, central Anatolia, using strontium isotope analysis of sheep tooth enamel and charred plant remains. Interpretation of strontium in sheep tooth sequences is constrained by previous oxygen isotope work, which largely excludes summer movement to the mountains but cannot distinguish between herding on the plain and the closest upland‐zone, Neogene limestone terraces. We establish a baseline contrast in modern plant strontium values between the plain and terraces and infer predominant herding on the plain from seven sheep tooth sequences. Archaeobotanical plant strontium values exclude the use of the terraces for cultivation and foraging. Relatively local crop and sheep management, plausibly intensive and integrated to some degree, given limited dry ground, appears likely on the basis of this pilot study.     

Diameter of the human internal acoustic meatus and sex determination

We report the results of testing the diameter of the internal opening of the acoustic canal in the petrous part of the temporal bone for sex determination of skeletal remains. The method involves measuring the diameter using a suite of ordinary drills. The method is very simple and has the great advantage of utilising one of the sturdiest bone elements of the human skeleton. The method may be especially useful for the analyses of very fragmented skeletal remains or cremated bones, where the petrous bone may still be readily recognisable. The method was tested using a forensic sample of 113 left petrous bones with known sex. Intra‐ and inter‐observer testing was also performed. We found a statistically significant difference in diameter between males and females (means: males: 3.7 mm; females: 3.4 mm; P < 0.009). However, the low predictive value (70%) for correct sexing using two sectioning points ( < 3.0 mm = female; >3.5 mm = male) was disappointing. No additional accuracy was gained by employing both left and right petrous bones (a bilateral sample of 60 petrous bones was also tested), although left and right side diameter is highly correlated (R = 0.778; P = 0.0001). Copyright © 2005 John Wiley & Sons, Ltd.