Stable isotope evidence of human diet at the Nukdo shell midden site,South Korea

Carbon and nitrogen stable isotope analysis was undertaken on bone collagen extracted from archaeological human (n = 48) and animal (n = 45) skeletons from the Nukdo site, Location I C, South Korea. This shell midden and grave site is dated from the late Mumun (550–300 BC) to early Iron Age (300 BC-1 AD) periods. The herbivorous mammals fell within the range of C₃ consumers, with average values of δ¹³C = −21.0 ± 0.5‰ and δ¹⁵N = 3.6 ± 0.5‰ for deer (n = 16) and δ¹³C = −20.6 ± 0.5‰ and δ¹⁵N = 4.5 ± 2.0‰ for wild boar (n = 17). Humans from this site averaged δ¹³C = −18.3 ± 0.4‰ and δ¹⁵N = 11.2 ± 0.7‰ for adults (n = 15) and δ¹³C = −18.7 ± 0.7‰ and δ¹⁵N = 12.5 ± 1.1‰ for juveniles (n = 33). These δ¹³C values indicate that there was no significant input of C₄ plants in the human diets and this may be associated with the spread of rice agriculture in the Mumun period. Human bone collagen δ¹³C and δ¹⁵N values indicate that there was some consumption of marine foods, although the main protein sources were from terrestrial foods. The isotope data demonstrate that the humans at Nukdo had mixed diets that included marine and terrestrial protein, including C₃ plants such as rice. Finally, the isotope results from the juveniles indicate that weaning occurred before the age of 1.5 years in this period.     

Stable isotopic analysis of human bones from Jiahu site,Henan, China: implications for the transition to agriculture

We have investigated change in subsistence during the transition to agriculture in the site of Jiahu, Henan Province, China, using stable isotopic analysis of collagen and apatite in human bones. Millet agriculture is well documented at drier high latitudes of the Yellow River Valley, while rice agriculture predominated at wetter lower latitudes of the Yangtze Valley region. The early Neolithic site of Jiahu lies near the boundary between the drier north and wetter south. Archaeobotanical evidence shows that rice was a significant component of diet at Jiahu, but its δ¹³C value is similar to that of other foods, and therefore cannot be conclusively identified by carbon isotope analysis. Foxtail and broomcorn millets are the only C₄ crops known for the Chinese Neolithic. Because of their high δ¹³C values, their consumption can be evaluated by stable carbon isotope analysis of human bone. Collagen reflects mainly the δ¹³C value of dietary protein, and apatite accurately records that of the whole diet. Isotopic analysis of 15 well-preserved samples from three periods shows that collagen δ¹³C values were very low for almost all individuals, suggesting C₃-based foods dominated their diets. However, apatite carbonate δ¹³C values and δ¹³C spacing between collagen and apatite (Δ¹³C_ap-co) indicate that millet may have been a minor component of the diet in this region. Individuals, who consumed the smallest amounts of animal protein, as indicated by low δ¹⁵N, generally had the highest apatite δ¹³C values. Archaeobotanical evidence for millet at Jiahu is needed to support this interpretation.     

A palaeodietary investigation of carbon (C/C) and nitrogen (N/N) in human and faunal bones from the Copper Age cemeteries of Varna I and Durankulak,Bulgaria

Stable isotope analyses have been applied to human and faunal bone collagen from the Varna I and Durankulak cemeteries to explore palaeodietary adaptations in the Neolithic and Eneolithic (Copper Age). The results suggest both populations primarily utilised terrestrial, C₃-based diets, despite their proximity to the Black Sea. The wider δ¹⁵N range of the Durankulak humans likely indicates the differential utilisation of terrestrial meat sources, which is probably related to the degree to which primary and/or secondary ovicaprid products were consumed, particularly since ovicaprid δ¹⁵N values differ from other herbivores. The isotopic distribution of Varna I reflects a linear relationship between δ¹⁵N and δ¹³C, suggesting that a minority of individuals enriched in both isotopic parameters supplemented their diets with marine resources. These burials include the well known ‘chieftain’ (burial 43) and show notable material wealth by way of grave goods. At the population level, however, there is no significant correlation between stable isotope values and material wealth at Varna I, a fact with implications for theories regarding emergent social/economic hierarchies in Balkan prehistory. Five burials at Durankulak were found to have relatively enriched δ¹³C and δ¹⁵N values with respect to the rest of the population. These burials reflect a prominently marine-based or mixed terrestrial C₃-based diet that included C₄ inputs, possibly from millet, for which the limitations of stable isotope analysis on bulk collagen are not able to differentiate. AMS dating has shown that these burials belong to a much later period.     

Stable carbon and nitrogen isotope analysis on human remains from the Early Mesolithic site of La Vergne (Charente-Maritime,France)

We report here the results of stable carbon and nitrogen isotope analysis of human and faunal remains from La Vergne (Charente-Maritime, western France), a rare Early Mesolithic burial site (ca. 8500–8000 cal BC). The results for nine humans (average δ¹³C = −19.3‰; δ¹⁵N = 9.4‰) indicate a strongly terrestrial diet, dominated by animal protein, with the possibility of, at best, a slight contribution of marine-derived protein. Given lower sea-levels in the early Holocene, the site would have been some 60–80 km from the sea at the time of its use; nevertheless, contacts with the coast are shown by the presence of numerous marine shell beads in the graves. In the light of the stable isotope results, it is suggested here that such contacts most likely took the form of exchange with coastal communities whose remains now lie underwater.     

Stable isotopic evidence for diet in a Roman and Late Roman population from Leptiminus,Tunisia

This study investigates the diet of the Roman and Late Roman population of Leptiminus on the Mediterranean coast of Tunisia. Stable carbon and nitrogen isotope analysis of human bone collagen and carbonate samples obtained from individuals buried in four cemeteries at Leptiminus was conducted in order to reconstruct the diet of the population, investigate the relative importance of marine vs. terrestrial resources, explore sex-, age-, and status-based variations in diet, examine temporal changes in the types of foods consumed, and compare the diet at Leptiminus with that of other Roman populations. The results of this study indicate that the residents of Leptiminus consumed a diet that was heavily reliant on terrestrial plant resources with the addition of a significant amount of marine resources. There were no significant sex differences in isotope values. In contrast, distinct dietary differences were seen between the adults and children. Nitrogen isotope values suggest that weaning began before the age of two and was completed by about 3 years of age, a finding consistent with previous isotopic studies of Roman samples. A temporal shift in diet is suggested by the nitrogen isotope values measured in samples from the most recent cemetery. A comparison of the data from Leptiminus with that derived from other Roman sites indicates that regional variability in diet existed within the Empire.     

Stable isotope analysis of Late Upper Palaeolithic human and faunal remains from Grotta del Romito (Cosenza), Italy

Stable isotope analysis of carbon (δ¹³C), nitrogen (δ¹⁵N) and sulphur (δ³⁴S) were carried out on one of the largest assemblages of Late Upper Palaeolithic human remains in Southern Europe, at Grotta del Romito (Cosenza), Italy. The burials were stratigraphically dated from ca. 18,000 to 13,000 cal BP, which was confirmed by a series of new AMS dates made directly on the bone collagen. Dietary reconstruction from carbon and nitrogen stable isotopes revealed that eight of the nine individuals analysed, dating to the Final Epigravettian, had very consistent diets, rich in terrestrial animal protein, regardless of their age or sex. These included two individuals who were suffering from severe pathologies. A single individual, dating to the Evolved Epigravettian had a more variable diet, which was significantly enriched in protein from marine or freshwater fish compared to the later burials. Overall, the results are consistent with the very limited number of other studies which describe a change to more specialised and less variable subsistence strategies, in this case the hunting of large herbivores, towards the end of the Palaeolithic period. Sulphur isotope values of all of the nine burials and several faunal samples were notably consistent, showing no evidence of long-distance migration to the site from a different geological zone.     

Stable isotope analysis of humans from Xiaojingshan site: implications for understanding the origin of millet agriculture in China

Millet agriculture originated in Northern China in the early Neolithic period (ca. 8000 BP), however, the actual importance of millet in human diets is still not clear. To determine the relative contribution of millet in human diets in this period we undertook stable isotope analysis of humans from Xiaojingshan site and fauna from Yuezhuang site, both of which are attributed to the Houli Culture and date to about 8000 years ago. The carbon isotope values of human bone collagen showed that millet (as a C₄ plant) only contributed approximately 25% of dietary protein, with the rest from C₃ based plant and animal sources, if a simple mixing model is used. We did not observe any statistical dietary difference between males and females at the site, although it has been argued that the Houli Culture was a matriarchal society. Finally, we compared our data with other published isotopic data from the contemporary Jiahu site and Xinglongwa site and a number of sites from the subsequent Yangshao Culture and found that millet only became a significant source of dietary protein approximately 1000 years later, as human carbon isotope values from these later sites indicated that almost all of dietary protein came from C₄ (i.e. millet) sources.     

Prehistoric Fijian diet and subsistence: integration of faunal, ethnographic, and stable isotopic evidence from the Lau Island Group

We explore subsistence practices and dietary change on islands in the central Lau Group of Fiji with zooarchaeological methods and stable isotopic analysis of human and animal skeletal material interpreted through an ethnoarchaeological lens. Our dataset combines detailed identifications of fauna, especially fishes, with stable isotopic (δ¹³C, δ¹⁵N) values of human and animal bone collagen and apatite carbonate spanning approximately three thousand years of human occupation on three study islands. Additionally, over fifty inshore and offshore contemporary fishing expeditions were observed on all study islands over a six-year period. We integrate these separate lines of evidence to form robust interpretations of Lauan subsistence patterns over a broad temporal scale. We add to the existing literature on stable isotopic analysis of archaeological bone from Remote Oceania and compare all of these groups through time. Our results indicate that Lauans differentially relied on nearshore reef resources rather than pelagic fishes, and terrestrial endemic species may have served as a portion of the diet during the early prehistoric period. Root crops (e.g., taro, yam) provided the majority of calories to the diet; however, sea grapes likely contributed to the early diet. Our isotopic results differ from previous studies of Fijian diet and that of Remote Oceania at a time of probable marine ecosystem shifts (AD 1300) illustrating diet breadth and variability in subsistence strategies potentially due to climatically influenced resource depletion.     

Late Stone Age subsistence in the Tilemsi Valley, Mali: Stable isotope analysis of human and animal remains from the site of Karkarichinkat Nord (KN05) and Karkarichinkat Sud (KS05)

The pathways leading to the adoption of cereal cultivation and pastoralism in West Africa are poorly understood. In order to elucidate the transition to food production during the Late Stone Age in Mali’s Tilemsi Valley samples of ancient and modern human and animal remains were selected for carbon and oxygen isotope analysis. Our results indicate the inhabitants of Karkarichinkat Nord (KN05) consumed considerable quantities (85%) of carbon derived from C₄ plants, either directly in the form of C₄ grasses such as wild Panicum sp. and possibly domestic Pennisetum sp. or indirectly through the consumption of C₄ grazers such as Bos sp. and Ovis sp.     

Early Neolithic diets at Baijia,Wei River valley,China: stable carbon and nitrogen isotope analysis of human and faunal remains

Stable carbon and nitrogen isotope values are presented for faunal and human bone collagen from Baijia, in the Wei River valley region of Shaanxi Province, China. The remains have a calibrated age range of ca. 5709–5389 BC, and correspond with the early Neolithic Laoguantai Period. Stable isotopic results indicate that human diets included millet and probably aquatic foods such as fish and shellfish. Bovid samples are tentatively identified as water buffalo, and have a mean δ¹³C value of −14.6‰, which reflects some millet consumption. Whether bovids were grazing on wild millet, or had diets directly influenced by humans, is not known. The single Sus sample from Baijia had a diet dominated by C3 plants and is thus unlikely to have been a domesticated animal. Overall, the stable isotope results presented here conform to the current concept that the people of the Laoguantai culture were millet farmers, who had subsistence strategies that included hunted wild foods.     

Subsistence and mobility strategies in the Epipalaeolithic: a stable isotope analysis of human and faunal remains at 'Uyun al-Hammam,northern Jordan

This paper investigates evidence for subsistence and settlement activities in the Levantine Middle Epipalaeolithic through the application of stable isotope analysis to human and non-human remains from the site of 'Uyun al-Hammam, northern Jordan. In general, bone from the site suffers a high degree of diagenesis and collagen could not be extracted for analysis here. Carbon and oxygen isotopic values from human tooth enamel samples were variable, but within expected values for the Middle Epipalaeolithic, whereas animal carbon and oxygen isotopic values varied widely, most likely due to hydrological and climatological factors. Carbon and isotopic values for the human samples indicated a predominantly C₃ plant dietary input, while animal samples appeared to have varying amounts of C₄ inputs into their diet. This is the first isotopic analysis conducted on material from the Middle Epipalaeolithic of the southern Levant and, as such, even results constrained by temporal and climatological variants within the region contribute to the overall knowledge of settlement and subsistence strategies during this cultural period.     

Mobility or migration: a case study from the Neolithic settlement of Nieder-Mörlen (Hessen,Germany)

A combination of stable carbon and nitrogen isotope analysis of collagen and radiogenic strontium isotope analysis of tooth enamel was used to investigate diet and mobility at the early Neolithic settlement of Nieder-Mörlen in Germany. The carbon and nitrogen ratios suggest a mixed terrestrial based diet that is consistent with data previously published for early Neolithic sites in Europe. The strontium isotope data indicate a high degree of human mobility with only one individual having an isotope ratio consistent with locally derived strontium. Unusually, a group of non-local juveniles with isotope ratios typical of upland regions is also present at the settlement but there are no adult burials with such values. Whilst transhumance is considered as an explanation, it would not explain why these non-local juveniles lived foreshortened lives and other possible mechanisms are therefore discussed.     

Stable isotopes and diet at Ancient Kerma,Upper Nubia (Sudan)

Carbon and nitrogen stable isotope values were measured from bone collagen extracted from archaeological Nubian human (n = 54) and faunal (n = 61) populations from the site of Kerma, Sudan. Collagen suitable for isotopic analysis was extracted from 22 faunal and 48 human samples from the Eastern cemetery site, dated to the Middle Kerma (c. 2050–1750 BC) and Classic Kerma (c. 1750–1500 BC) periods respectively. The isotopic data indicate that the human dietary regimen included a mix of C₃ and C₄ plant-derived components, with a larger C₄ component than previously reported in archaeological Egyptian Nile Valley populations. Elevated δ¹⁵N values are attributed to consumption of dietary resources from a ¹⁵N-enriched terrestrial ecosystem. The faunal isotope data also indicate the consumption of C₃ and C₄ plants. The large range of δ¹³C values measured in both the human and faunal samples supports previous work suggesting that a significant portion of the populations buried at Kerma may have originated elsewhere, further confirming the Nile Valley as a major corridor for population movement in the region.     

Stable isotope investigations of charred barley (Hordeum vulgare) and wheat (Triticum spelta) grains from Danebury Hillfort: implications for palaeodietary reconstructions

Palaeodietary studies typically focus on the analysis of bone collagen due to the limited availability of plant remains. Isotopic analysis of plant remains, however, allow for a more extensive consideration of the contribution of plants to the human diet and can potentially provide information about the environment in which the crops were grown. This paper reports the results of carbon and nitrogen isotope analyses performed on charred barley and wheat grains recovered from pits within Danebury Iron Age hillfort. To the best of our knowledge, this is the first Iron Age site in Britain from which charred grains have been isotopically analysed. Our results suggest that cereals found at the hillfort were grown in several different environmental contexts. The isotope data demonstrate that the herbivores were not consuming a diet primarily based on grains as the δ¹⁵N values of the grains are very similar to those of the herbivores. Palaeodietary investigations typically assume that humans eating plant protein only would have the same δ¹⁵N value as the local herbivores. This assumption is clearly invalid at Danebury, where the humans and animals appear to have consumed either different parts of the same plants or different plants. Researchers typically interpret high differences between human and animal δ¹⁵N values as indicative of diets high in animal protein, however where major plant resources have δ¹⁵N values similar to those of the herbivores our ability to distinguish between plant and animal sources of protein in the diet is limited. Our research has demonstrated that whenever possible it is desirable to measure the isotopic signatures of potential major plant resources in order to understand past subsistence strategies.     

Mummies, maize, and manure: multi-tissue stable isotope analysis of late prehistoric human remains from the Ayacucho Valley, Perú

This paper reports the δ¹³C and δ¹⁵N values of bone collagen, muscle and skin from several late prehistoric–early colonial (AD 1490–1640) mummies from Perú's Ayacucho Valley. The mean of the δ¹³C and δ¹⁵N values of bone collagen are −11.5 ± 1.4 and 11.1 ± 0.7‰, respectively. The mean of the δ¹³C values for Vinchos skin is −11.8 ± 1.2‰ and the mean of δ¹⁵N values is 13.2 ± 0.5‰. The samples of muscle tissue have a δ¹³C mean of −11.9 ± 0.9‰ and a δ¹⁵N mean of 12.7 ± 0.3‰. The data from bone collagen indicate maize was the basis of the region's subsistence economy. A significant correlation between δ¹³C and δ¹⁵N values of bone collagen (R² = 0.75) is consistent with the preferential fertilization of maize with composted manure. Both skin and muscle samples are consistently enriched in δ¹⁵N relative to paired samples of bone (2.1 ± 0.5 and 1.6 ± 0.7‰, respectively), possibly as a result of short term physiological stress or differential decomposition.     

Wild at heart: Approaching Pitted Ware identity,economy and cosmology through stable isotopes in skeletal material from the Neolithic site Korsnäs in Eastern Central Sweden

The Middle Neolithic Pitted Ware Culture on the Baltic Sea islands comprised a common identity distinguished, in part, by an almost exclusively marine diet. Based on evidence from the first stable isotope analysis on Pitted Ware skeletal material from the Eastern Central Swedish mainland, we suggest that this identity was shared by PWC groups in the archipelago of the west side of the Baltic. Fifty-six faunal and 26 human bone and dentine samples originating from the Pitted Ware site Korsnäs in Södermanland, Sweden were analysed, and the data clearly shows that the diet of the Korsnäs people was marine, predominantly based on seal. The isotope data further indicate that the pig bones found in large quantities on the site emanate from wild boar rather than domestic pigs. The large representation of pig on several Pitted Ware sites, which cannot be explained in terms of economy, is interpreted as the results of occasional hunting of and ritual feasting on wild boar, indicating that the animal held a prominent position, alongside seal, in the hunting identity and cosmology of the Pitted Ware people. Further, eleven new radiocarbon dates are presented, placing the Korsnäs site, with a large probability, within Middle Neolithic A.     

Stable isotope analysis of human and animal remains from the Late Upper Palaeolithic site of Balma Guilanyà, southeastern Pre-Pyrenees,Spain

Stable isotope analysis of carbon (¹³C/¹²C) and nitrogen (¹⁵N/¹⁴N) was performed on collagen extracted from three human and five herbivore bone and tooth samples from the Late Upper Palaeolithic site of Balma Guilanyà (Catalonian Pre-Pyrenees, Spain). Contextual and palaeoecological data as well as radiocarbon dates indicate that the studied occupation phase took placed during the Bolling/Allerod interstadial (GI-1a event). The human remains were co-mingled without any anatomical association, corresponding to a minimum number of three individuals, and it was not possible to determine if the three analyzed samples are from one or more individuals. The mean isotope values obtained from the human remains are δ¹³C = −19.8‰ and δ¹⁵N = 6.7‰, while those of the large herbivores (red deer and wild goat) were −19.8‰ and 1.7‰ for δ¹³C and δ¹⁵N respectively. This indicates that the main source of protein in the diet of the Balma Guilanyà human(s) came from terrestrial herbivores. There is no zooarchaeological or isotopic evidence for the consumption of freshwater or marine resources at the site, which lies 80 km from the present Mediterranean coast. The low δ¹⁵N values observed in both human and animal samples correspond to a trend reported by other researchers working in northwestern Europe: a significant δ¹⁵N reduction in collagen from bones datable within 20,000–10,000 BP, followed by a rise to present values in the Early Holocene. This phenomenon, generally attributed to climatic and/or pedological processes, had not been previously observed in the Mediterranean region and, until now, was thought to be restricted to northern Europe.     

‘Rid grasse of bones’: A taphonomic study of the bones from midden deposits at the Neolithic and Bronze age site of Runnymede,Surrey, England

Runnymede has large samples of Neolithic and Late Bronze Age animal bones, with contrasting preservation conditions in both periods. The bone evidence has been used to interpret the formation of the site deposits. There are few articulated bones, and no joins were found in butchered bone, indicating that the area studied did not contain primary refuse. Various aspects of bone alteration have been analysed: (i) the proportion of bones with very good surface preservation was high in the in situ Neolithic excavation units and the basal Bronze Age midden, but bones in the upper units were mostly eroded. These units are reworked flood deposits. The greater degree of fragmentation of the bone in the reworked units has been quantified, using a system of recording the ‘zones’ present on each bone, which allows calculation of the fraction present. It is also demonstrated that the reworked units contain a lower proportion of identified bones and a higher proportion of teeth and iaws than the units with well-preserved bone, (ii) Quantification of canid gnawing shows, unexpectedly, that more was recorded on well-preserved bone. Thus recognition of gnawing depends on bone condition. This also confirms that most of the erosion of the bone surface is a post-depositional phenomenon. The sequence of activities is therefore interpreted as follows: meat was cooked and consumed, and the bones discarded for the dogs. At a later stage, larger bones were picked up and thrown away in the river or midden. Some ethnographic examples of periodic cleaning of farming settlements are cited.     

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Fifty-eight dental calculus samples from medieval and post-medieval skeletons from Vitoria, Spain, and a single sample from an Alaskan Inuit were tested for stable carbon and nitrogen isotope compositions. There was sufficient carbon and nitrogen concentrations to obtain δ¹³C and δ¹⁵N values, and the samples from Spain produced results that were replicable and comparable to European isotope values based on bone collagen collected from literature sources. The Alaskan Inuit calculus sample yielded a δ¹⁵N value of +17.5‰, well beyond the range of the Spanish samples, but consistent with literature data for modern Greenlandic Inuit consuming a diet rich in marine food. There are several potential sources for carbon and nitrogen in calculus. The results of this study yield stable isotope values consistent with those obtained from other biomaterials used as isotope proxies for paleodietary research, including bone collagen, hair, and fingernails, although further work is necessary to verify the fidelity of calculus as an isotope proxy. Many studies in bioarchaeology are precluded by curatorial concerns regarding the destructive analysis of primary biomaterials. However, calculus is an “add-on”, or secondary biomaterial, that is not an integral part of the dental or skeletal system. Hence, its consumption during analysis is technically not destructive. Therefore, isotope analysis of dental calculus may provide a potential new avenue for paleodietary analysis where the use of other primary biomaterials is precluded.     

Diet and mobility in Middle Archaic Florida: stable isotopic and faunal evidence from the Harris Creek archaeological site (8Vo24), Tick Island

We explore diet and mobility in Middle Archaic Florida using human burials and faunal remains from the Harris Creek archaeological site (8Vo24) on Tick Island. We conducted stable carbon and oxygen isotopic (δ¹³C, δ¹⁸O) analyses of 50 human enamel samples and strontium isotopic (⁸⁷Sr/⁸⁶Sr) analysis of a subset of 10. Most individuals (46/50) subsisted on local freshwater and terrestrial resources, with relatively depleted δ¹³C values (C₃) and δ¹⁸O values matching those of central and north Florida water resources within the St. Johns River valley. Eight of 10 burials have local, within valley ⁸⁷Sr/⁸⁶Sr values. Two human burials yield marine δ¹³C and ⁸⁷Sr/⁸⁶Sr values, which we interpret as evidence for across valley movement from the coast. Another two individuals show depleted δ¹⁸O values, likely obtained from northern rivers. We also identified potential food resources and non-local foods from the Harris Creek faunal assemblage. We found six species with estuarine and marine habitat preferences; two of which, whelk and quahog, indicate that people of Harris Creek traveled to or traded with coastal areas.