Dietary adaptation during the Longshan period in China: stable isotope analyses at Liangchengzhen (southeastern Shandong)

Rice and millet were staple crops at Liangchengzhen, a late Neolithic Longshan site in Shandong, China, but the degree of dietary variation is not known. This study uses stable isotope analysis of human and faunal skeletal remains to quantitatively address the importance of these crops as well as terrestrial domesticates and aquatic resources in the diet at Liangchengzhen. Although no collagen could be extracted from the poorly preserved human bones, the δ¹³C stable isotope results for 2 apatite sample and 16 tooth enamel samples averaged −9.8‰ suggesting that diet was based on foods averaging from −24‰ to −18‰, with millet and millet-fed animals comprising at most approximately 25–30% of the diet. Pig faunal δ¹³C isotope values suggested that during the earlier Longshan period pigs were fed mainly millet with more C₃ foods such as rice included by the later Longshan period. Solid ceramic residues from two guan jar sherds produced δ¹³C values averaging −18‰ and δ¹⁵N values averaging +16‰, suggesting both vessels contained fish. The results of the study indicate that by the Longshan period, people in southeastern Shandong no longer relied as heavily on millet and that the agricultural crop of rice had increased in importance at Liangchengzhen. Unfortunately, without human collagen samples to provide nitrogen isotope results, we cannot estimate the relative contribution of aquatic and terrestrial protein to the diet of people at Liangchengzhen. In general, however, the pattern of a diverse agricultural system on the basis of the macrobotanical remains from Liangchengzhen is supported by the isotopic results.     

Migration and Viking Dublin: paleomobility and paleodiet through isotopic analyses

During the early medieval period in Ireland, Dublin was established as the largest Viking settlement on the island in the ninth century AD. A previous biodistance study has suggested that the population of the town consisted of a polyethnic amalgam of immigrant and indigenous. In this study, we use biogeochemistry to investigate paleomobility and paleodiet in archeological human remains from the ninth to eleventh century levels at the sites at Fishamble Street II (National Museum of Ireland excavation number E172), Fishamble Street III (E190) and John’s Lane (E173), as well as twelfth-century remains from Wood Quay (E132). Through radiogenic strontium isotope, stable oxygen, carbon, and nitrogen isotope, and elemental concentration analyses, we investigate the origins of the individuals who lived and died in early and late Viking Dublin. Mean archaeological human enamel and bone isotope values from Dublin are ⁸⁷Sr/⁸⁶Sr = 0.70975 ± 0.00139 (2σ, n = 22), δ¹³C_{carbonate(V-PDB)} = −14.8‰ ± 0.8‰ (1σ, n = 12), and δ¹⁸O_{carbonate(V-PDB)} = −7.2‰ ± 1.0‰ (1σ, n = 12). Archaeological human bone samples exhibit mean δ¹³C_{collagen(V-PDB)} = −20.8‰ ± 0.5‰ (1σ, n = 12) and mean δ¹⁵N_{collagen(AIR)} = +10.0‰ ± 1.7‰ (1σ, n = 12). Comparing these data with archaeological faunal data from Dublin and published data from northern Europe, we argue that there are no clear immigrants from other parts of the North Atlantic, although there is one clear outlier in both origins and diet. Overall, the relative homogeneity in both paleomobility and paleodiet may support models of acculturation in Viking Dublin, rather than a high number of first-generation immigrants or continued migration from Scandinavia.     

Provisioning Inka feasts at Tiwanaku, Bolivia: the geographic origins of camelids in the Pumapunku complex

While political integration can be achieved by many means, here we focus on the use of feasting and statecraft in the Inka Empire of the Andean Late Horizon (c. AD 1400-1532) in South America. In order to examine Inka political integration of the Lake Titicaca Basin of Bolivia, we examine paleomobility and paleodiet through radiogenic strontium and stable oxygen and carbon isotope data in archaeological camelid remains from the site of Tiwanaku. Mean radiogenic strontium isotope values from all archaeological camelid enamel and bone samples is ⁸⁷Sr/⁸⁶Sr = 0.70998 ± 0.00179 (1σ, n = 48), mean stable oxygen isotope values from a sub-set of archaeological camelid enamel and bone samples is δ¹⁸O_{carbonate (VPDB)} = −10.0‰ ± 2.6‰ (1σ, n = 18) and mean stable carbon isotope values from a sub-set of archaeological camelid enamel and bone samples is δ¹³C_{carbonate (VPDB)} = −9.0‰ ± 1.7‰ (1σ, n = 18). While many camelids consumed in these feasting events were likely local to the Lake Titicaca Basin, others came from a variety of different geologic zones, elucidating our understanding of Inka statecraft and the role of feasting in political integration in empires in the past.     

Ascetic or affluent? Byzantine diet at the monastic community of St. Stephen’s,Jerusalem from stable carbon and nitrogen isotopes

Stable carbon and nitrogen isotope ratios from bone collagen in skeletons from the Byzantine (5th–7th century AD) monastery of St. Stephen’s in Jerusalem were examined in conjunction with a review of historical sources detailing dietary practices during this period in the Levant. Relatively low δ¹³C ratios (−19.0 ± 0.5‰, 1σ) indicate a diet consisting primarily of C₃ sources and display continuity with textual records describing monastic daily life. Conversely, human δ¹⁵N values (9.6 ± 1.2‰, 1σ) are enriched in ¹⁵N relative to local fauna (7.3 ± 1.1‰, 1σ) and point to the contribution of animal protein to the diet, an unexpected result based on both the rarity and expense of these luxury food items as well as dietary prohibitions associated with an ascetic monastic lifestyle. No sex-based differences in diet were detected for either δ¹³C or δ¹⁵N values, suggesting that men and women consumed isotopically similar foods. As the vast majority of monastic communities in the ancient Near East were located in the desert, the urban setting of St. Stephen’s monastery allows for a unique glimpse into a rarely-explored facet of Byzantine life.     

Stable isotope evidence of human diet at the Nukdo shell midden site,South Korea

Carbon and nitrogen stable isotope analysis was undertaken on bone collagen extracted from archaeological human (n = 48) and animal (n = 45) skeletons from the Nukdo site, Location I C, South Korea. This shell midden and grave site is dated from the late Mumun (550–300 BC) to early Iron Age (300 BC-1 AD) periods. The herbivorous mammals fell within the range of C₃ consumers, with average values of δ¹³C = −21.0 ± 0.5‰ and δ¹⁵N = 3.6 ± 0.5‰ for deer (n = 16) and δ¹³C = −20.6 ± 0.5‰ and δ¹⁵N = 4.5 ± 2.0‰ for wild boar (n = 17). Humans from this site averaged δ¹³C = −18.3 ± 0.4‰ and δ¹⁵N = 11.2 ± 0.7‰ for adults (n = 15) and δ¹³C = −18.7 ± 0.7‰ and δ¹⁵N = 12.5 ± 1.1‰ for juveniles (n = 33). These δ¹³C values indicate that there was no significant input of C₄ plants in the human diets and this may be associated with the spread of rice agriculture in the Mumun period. Human bone collagen δ¹³C and δ¹⁵N values indicate that there was some consumption of marine foods, although the main protein sources were from terrestrial foods. The isotope data demonstrate that the humans at Nukdo had mixed diets that included marine and terrestrial protein, including C₃ plants such as rice. Finally, the isotope results from the juveniles indicate that weaning occurred before the age of 1.5 years in this period.     

Paleodiet in northern Chile through the Holocene: extremely heavy δN values in dental calculus suggest a guano-derived signature?

Dental calculus extracted from the teeth of 28 prehistoric human skeletons from coastal and valley archaeological sites in northern Chile that date from the Archaic period (∼2300 BC) to the Late Intermediate period (AD 1476) was analyzed for stable isotope (δ¹⁵N, δ¹³C) compositions. δ¹⁵N compositions were extremely heavy (+17.8 to +33.1‰), but comparable to some studies using conventional human biomaterials (hair, nail, muscle) at other prehistoric sites in northern Chile. There was a negative correlation between δ¹⁵N and δ¹³C for coastal sites, but a positive correlation for valley sites. Results for the valley sites point to a diet that was influenced by marine resources throughout all time periods. The unusually heavy δ¹⁵N values for the coastal sites require a dietary component with a δ¹⁵N composition significantly heavier than that of marine resources. The hyper-aridity at the study area (mean annual rainfall of 0.5–0.6 mm/year) is a likely contributing factor to the production of heavy δ¹⁵N values, but is unlikely to account for the heaviest δ¹⁵N values (>+30‰). One possible explanation for the heaviest δ¹⁵N values is that dietary components were impacted by isotopically-heavy guano, which is abundant in the region. Guano may have been used as a fertilizer during crop cultivation at the onset of the Formative period, continuing through the Historic period after Spanish contact. The indirect impact of guano from abundant bird habitats in the region may have influenced wild foodstuffs harvested throughout the pre-agricultural period. Results provide support for the utility of dental calculus as a proxy for obtaining stable carbon and nitrogen isotope signatures for use in paleodietary studies.     

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Fifty-eight dental calculus samples from medieval and post-medieval skeletons from Vitoria, Spain, and a single sample from an Alaskan Inuit were tested for stable carbon and nitrogen isotope compositions. There was sufficient carbon and nitrogen concentrations to obtain δ¹³C and δ¹⁵N values, and the samples from Spain produced results that were replicable and comparable to European isotope values based on bone collagen collected from literature sources. The Alaskan Inuit calculus sample yielded a δ¹⁵N value of +17.5‰, well beyond the range of the Spanish samples, but consistent with literature data for modern Greenlandic Inuit consuming a diet rich in marine food. There are several potential sources for carbon and nitrogen in calculus. The results of this study yield stable isotope values consistent with those obtained from other biomaterials used as isotope proxies for paleodietary research, including bone collagen, hair, and fingernails, although further work is necessary to verify the fidelity of calculus as an isotope proxy. Many studies in bioarchaeology are precluded by curatorial concerns regarding the destructive analysis of primary biomaterials. However, calculus is an “add-on”, or secondary biomaterial, that is not an integral part of the dental or skeletal system. Hence, its consumption during analysis is technically not destructive. Therefore, isotope analysis of dental calculus may provide a potential new avenue for paleodietary analysis where the use of other primary biomaterials is precluded.     

Stable isotope analysis of dog,fox, and human diets at a Late Holocene Chumash village (CA-SRI-2) on Santa Rosa Island,California

Stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope analyses of dog (Canis familiaris), island fox (Urocyon littoralis), and human bone collagen from CA-SRI-2 (AD 130–1830) on Santa Rosa Island, California provide a proxy of diet and the relationships between humans and these animals. Carbon isotopic signatures indicate that Native Americans and their dogs at CA-SRI-2 subsisted almost exclusively on marine resources, while the island fox ate primarily terrestrial foods. Nitrogen isotopes and archaeofaunal remains indicate that humans and dogs also ate higher trophic level foods, including finfishes, marine mammals, and seabirds with smaller amounts of shellfish. The CA-SRI-2 island foxes appear to have eaten higher amounts of terrestrial foods, similar to the diets observed in modern fox populations. These data generally confirm the commensal relationship assumed to exist between domesticated dogs and people, but the carbon isotopic composition of dogs is enriched ∼2‰ compared to humans. We hypothesize that the difference in carbon isotopes between dogs and humans may have resulted from a higher consumption of C3 plants with lower δ¹³C values by humans, or less likely from the ingestion by dogs of significant amounts of bone collagen, which is enriched by ∼4‰ over associated muscle.     

Human–environment interactions in medieval Poland: a perspective from the analysis of faunal stable isotope ratios

Stable isotope analyses of faunal remains provide valuable information about human–environment interactions in the past, including insights into past animal husbandry and land management strategies. Here, we report stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope values of collagen and carbonate from archaeological fauna from Ka?dus, a medieval settlement in North-Central Poland, to better understand human–environment interactions during a period of increasing urbanism and marketization. Wild and domestic animals can be separated on the basis of their isotopic values. The mean δ¹⁵N value for 12 domesticated animals is 7.6 ± 1.2‰ and for 5 wild animals is 4.3 ± 0.5‰ (p = 0.002). The mean collagen δ¹³C value for domesticated animals is −20.6 ± 1.1‰ and for wild animals is −22.0 ± 0.5‰ (p = 0.004). The mean carbonate δ¹³C value for domesticated animals is −13.14 ± 1.3‰ and for wild animals is −14.14 ± 0.9‰ (p = 0.034). The “canopy effect” and anthropogenic effects that alter stable isotope ratios of plants (manuring, swidden agriculture and ploughing) are discussed in relation to these differences. Fish are isotopically variable, which suggests broad-spectrum fishing strategies and/or trade, and increases our awareness of the difficulties in interpreting human paleodiet when freshwater fish were on the menu.     

Isotopically distinct modern carbonates in abandoned livestock corrals in northern Kenya

We report δ¹⁸O and δ¹³C data from modern carbonate in soils and dung samples from 3 recently abandoned livestock corrals in northern Kenya. Calcium carbonate content is higher within ∼5 cm depth that contains a mixture of dung and surface soils of corrals than in soils below 5 cm depth. We radiocarbon dated carbonates from 0.5 to 40 cm depths in two corrals and one control site. Surface carbonates (0.5 cm) from the two corrals were formed from modern carbon (>1955) when the corrals were active, while all other carbon is >16,000 years (BP) old. Shallow carbonate is also enriched in ¹⁸O (δ¹⁸O up to 3.0‰) and depleted in ¹³C (δ¹³C up to −12.0‰) with respect to carbonate at deeper levels and at two control sites. The δ¹⁸O and δ¹³C of soil carbonates (δ¹⁸O_SC and δ¹³C_SC respectively) in corrals are inversely correlated for depths up to about 15 cm where organic carbon is greater than 0.5%. Below that depth, there is a positive correlation between δ¹⁸O_SC and δ¹³C_SC values, similar to that observed in a control site.     

Nitrogen isotopes and the trophic level of humans in archaeology

Human and domesticate animal bone collagen δ¹⁵N values in prehistory differ generally by 3‰ or more from Neolithic to post- Roman times in Northwest Europe, leading to an assumed dietary animal protein fraction of 60–80% using a standard interpretation of δ¹⁵N values. We examine the assumptions on which this model rests and the limitations of our knowledge in the analysis of δ¹⁵N values in archaeology. We have developed a set of models which, with small changes made in assumptions (on the order of 1‰), can produce substantially lower estimates of the dietary animal protein fraction for given δ¹⁵N values. We consider the implications of various dietary animal protein fractions on agricultural carrying capacities and human population densities in prehistory.     

Diagenesis in bone and enamel apatite carbonate; the potential of density separation to assess the original composition

This paper presents a new approach to the isotopic measurement of diagenetically altered archaeological bone apatite carbonate. We describe how the existing differential dissolution method may be combined with a new approach to remove diagenetically reformed material on the basis of its greater specific gravity. We show that heavier, more diagenetically altered fraction has a higher (altered) δ¹³C and also increased crystallinity within one individual. In addition, we proposed one potential tool to check the validity of bone carbonate by comparing bone collagen, enamel carbonate and bone carbonate values of δ¹³C and radiocarbon content from the same individual. In the case of Danebury cattle, we estimated the biogenic δ¹³C_apa value for DC83 and DC89, although it is still difficult to overcome diagenesis in order to meet the expected value, which is shown to be 1.9‰ (DC83) and 3.5‰ (DC89).     

Stable isotopic analysis of human and faunal remains from the Incipient Chulmun (Neolithic) shell midden site of Ando Island,Korea

We analyzed stable carbon and nitrogen isotope ratios of human and animal remains from the Ando shell midden, South Korea. The Ando site is a rare Incipient Chulmun (Neolithic) site (ca. 6000–5000 BC), which contains well-preserved human and animal bones in shell mounds. The stable isotope results for humans (average δ¹³C = −13.5 ± 0.5‰ and δ¹⁵N = 15.2 ± 0.5‰) indicate that Ando people in the Incipient Chulmun period strongly depended on marine resources. There were no isotopic differences between humans of different sex and age at this site. We compared our data with other previous published isotopic data from the Chulmun sites and found that the Ando people had similar isotope values to the southern Chulmun people (Tongsamdong and Daepo), but different isotopic ratios than the western Chulmun people (Daejukri and Konamri). These results indicate that marine foods were the main food resources in the southern coastal regions, but not in the western coastal regions in Chulmun Korea.     

Stable carbon and nitrogen isotope analysis on human remains from the Early Mesolithic site of La Vergne (Charente-Maritime,France)

We report here the results of stable carbon and nitrogen isotope analysis of human and faunal remains from La Vergne (Charente-Maritime, western France), a rare Early Mesolithic burial site (ca. 8500–8000 cal BC). The results for nine humans (average δ¹³C = −19.3‰; δ¹⁵N = 9.4‰) indicate a strongly terrestrial diet, dominated by animal protein, with the possibility of, at best, a slight contribution of marine-derived protein. Given lower sea-levels in the early Holocene, the site would have been some 60–80 km from the sea at the time of its use; nevertheless, contacts with the coast are shown by the presence of numerous marine shell beads in the graves. In the light of the stable isotope results, it is suggested here that such contacts most likely took the form of exchange with coastal communities whose remains now lie underwater.     

Stable isotope analysis of human and animal remains from the Late Upper Palaeolithic site of Balma Guilanyà, southeastern Pre-Pyrenees,Spain

Stable isotope analysis of carbon (¹³C/¹²C) and nitrogen (¹⁵N/¹⁴N) was performed on collagen extracted from three human and five herbivore bone and tooth samples from the Late Upper Palaeolithic site of Balma Guilanyà (Catalonian Pre-Pyrenees, Spain). Contextual and palaeoecological data as well as radiocarbon dates indicate that the studied occupation phase took placed during the Bolling/Allerod interstadial (GI-1a event). The human remains were co-mingled without any anatomical association, corresponding to a minimum number of three individuals, and it was not possible to determine if the three analyzed samples are from one or more individuals. The mean isotope values obtained from the human remains are δ¹³C = −19.8‰ and δ¹⁵N = 6.7‰, while those of the large herbivores (red deer and wild goat) were −19.8‰ and 1.7‰ for δ¹³C and δ¹⁵N respectively. This indicates that the main source of protein in the diet of the Balma Guilanyà human(s) came from terrestrial herbivores. There is no zooarchaeological or isotopic evidence for the consumption of freshwater or marine resources at the site, which lies 80 km from the present Mediterranean coast. The low δ¹⁵N values observed in both human and animal samples correspond to a trend reported by other researchers working in northwestern Europe: a significant δ¹⁵N reduction in collagen from bones datable within 20,000–10,000 BP, followed by a rise to present values in the Early Holocene. This phenomenon, generally attributed to climatic and/or pedological processes, had not been previously observed in the Mediterranean region and, until now, was thought to be restricted to northern Europe.     

A palaeodietary investigation of carbon (C/C) and nitrogen (N/N) in human and faunal bones from the Copper Age cemeteries of Varna I and Durankulak,Bulgaria

Stable isotope analyses have been applied to human and faunal bone collagen from the Varna I and Durankulak cemeteries to explore palaeodietary adaptations in the Neolithic and Eneolithic (Copper Age). The results suggest both populations primarily utilised terrestrial, C₃-based diets, despite their proximity to the Black Sea. The wider δ¹⁵N range of the Durankulak humans likely indicates the differential utilisation of terrestrial meat sources, which is probably related to the degree to which primary and/or secondary ovicaprid products were consumed, particularly since ovicaprid δ¹⁵N values differ from other herbivores. The isotopic distribution of Varna I reflects a linear relationship between δ¹⁵N and δ¹³C, suggesting that a minority of individuals enriched in both isotopic parameters supplemented their diets with marine resources. These burials include the well known ‘chieftain’ (burial 43) and show notable material wealth by way of grave goods. At the population level, however, there is no significant correlation between stable isotope values and material wealth at Varna I, a fact with implications for theories regarding emergent social/economic hierarchies in Balkan prehistory. Five burials at Durankulak were found to have relatively enriched δ¹³C and δ¹⁵N values with respect to the rest of the population. These burials reflect a prominently marine-based or mixed terrestrial C₃-based diet that included C₄ inputs, possibly from millet, for which the limitations of stable isotope analysis on bulk collagen are not able to differentiate. AMS dating has shown that these burials belong to a much later period.     

In situ oxygen isotope micro-analysis of faunal material and human teeth using a SHRIMP II: a new tool for palaeo-ecology and archaeology

A Sensitive High Resolution Ion MicroProbe (SHRIMP II) has been used to make high spatial resolution in situ micro-analyses of oxygen isotopes in fish otoliths, and teeth from fossil herbivores and a Neanderthal. Large intra-tooth variations in the oxygen isotopic composition (up to 9‰) were observed in the enamel of herbivores from the Neanderthal fossil site of Payre, consistent with preservation of seasonal cyclicity. The range of isotopic compositions observed in Neanderthal tooth enamel was much smaller (∼3‰), possibly the result of a longer enamel maturation time averaging out variability. An archaeological otolith from a Preceramic site in Northern Peru exhibited marked changes in δ¹⁸O over life, due either to the fish occasionally migrating from the sea to a lower salinity habitat, or to short-lived rises in sea water temperature. A fish otolith from Australia's Willandra Lakes World Heritage Area showed clear seasonal variations, but also a general trend towards isotopically heavier and more saline water, as indicated by higher δ¹⁸O and Sr/Ca values resulting from increased evaporation. The results of these case studies are compared to results of oxygen isotope analysis using more conventional methods and demonstrate the ability of the SHRIMP II to provide precise high spatial resolution in-situ oxygen isotope analyses of a variety of biogenic materials. This approach has major advantages over conventional methods. It can provide rapid, micro-scale isotopic analyses of sub-permil precision without the need for chemical preparation of the sample.     

Probing dietary change of the Kwädąy Dän Ts'ìnchį individual,an ancient glacier body from British Columbia: II. Deconvoluting whole skin and bone collagen δC values via carbon isotope analysis of individual amino acids

The remains of the Kwäd?y Dän Ts'ìnch? individual, a frozen male human, were recovered from a retreating glacier within the Tatshenshini-Alsek Park in British Columbia in August 1999. In order to provide information on both the geographical origin of this individual and low long he spent in the remote interior region prior to his death, molecular analysis and compound-specific carbon isotope analyses were performed on individual amino acids purified from his skin and bone. Gas chromatographic quantification of constituent amino acids of both tissues revealed a molecular distribution characteristic of collagen, dominated by glycine and to a lesser extent proline, hydroxyproline and alanine. Chiral gas chromatography indicated that protein preservation in both tissues was exceptional. Carbon isotope analysis of a faunal assemblage from an earlier prehistoric site from southern British Columbia provided reference dietary amino acid δ¹³C values for terrestrial (deer and domestic dog) and marine species (salmon and sealion), showing clear separation in all amino acids, particularly glycine which was extremely ¹³C-enriched in the marine animals. The distinction between terrestrial and marine organisms was increased by exploring Δ¹³C_{Glycine-Phenylalanine} values (6.6 ± 0.6‰ and 15.0 ± 2.1‰, respectively), which were higher in the latter by approximately 8‰, mirroring the increased δ¹⁵N_{Bulk collagen} values observed for the marine animals (R² = 0.78; p < 0.001). The Kwäd?y Dän Ts'ìnch? individual's bone had a similarly elevated Δ¹³C_{Glycine-Phenylalanine} value of 15.6 ± 1.0‰, indicating his extreme reliance on marine dietary resources throughout early life. The skin amino acid δ¹³C values were consistently lower than those observed for bone, with a concurrently lower Δ¹³C_{Glycine-Phenylalanine} value of 12.7 ± 0.9‰. The shift between the carbon isotope composition of bone (long-term diet) and skin amino acids (short-term diet) confirmed a sudden divergence away from marine food sources in the last months of life, consistent with his discovery 80 km inland.     

Feeding Management Strategies among the Early Neolithic Pigs in the NE of the Iberian Peninsula

The socio‐economic relevance of domesticated animals during the Early Neolithic in the Iberian Peninsula is indisputable, yet we essentially know little about the way they were managed. Among domesticated animals, pig (Sus domesticus ) was a common food source, and previous studies have shown the potential of stable isotopes for assessing variability in pig diet in relation to husbandry practices. Nevertheless, this approach has never been applied to the earliest pigs in the Iberian Peninsula. We analysed the carbon and nitrogen stable isotope composition of pig bone collagen from several Early Neolithic sites in the NE Iberian Peninsula. While pig δ¹³C values were similar across different populations, there were significant differences in δ¹⁵N values between sites. These are attributed to different pig husbandry systems, which may reflect distinct social and spatial organisation and interaction with environmental conditions during the Early Neolithic in this region. Copyright © 2017 John Wiley & Sons, Ltd.     

Stable Isotope Analysis of Diet‐based Social Differentiation at Late Prehistoric Collective Burials in South‐Western Portugal

In the Neolithic and Copper Age collective burials of the Portuguese Estremadura, the majority of material culture and skeletal remains are highly commingled, making it difficult for archaeologists to evaluate social status by linking individuals with specific grave goods. In these circumstances, bio‐anthropological data about individual life histories offer an additional avenue of investigation into social complexity among prehistoric communities practising collective burial. In this study, stable isotope data were gathered from 81 individuals from seven collective burial sites and one settlement, the fortified site of Zambujal, in order to determine if significant dietary differences exist within or between burials that may point to patterns of social differentiation, both at individual sites and across the region. While in general all of the sampled individuals consumed fairly homogeneous diets based on terrestrial animal proteins and C₃ plants, this study found that statistically significant differences in δ¹³C_ap and δ¹⁵N values exist between several sites, which may indicate socially differentiated consumption of meat and plants. Additionally, statistically significant differences were found in δ¹⁵N values between adults and juveniles, which may either be attributable to protein‐restrictive child‐feeding practices or physiological processes related to skeletal growth and development.