Interpreting cultural remains in airborne laser scanning generated digital terrain models: effects of size and shape on detection success rates

In this study, detection success rates were evaluated for cultural remains that were detected manually based on interpretation of digital terrain models (DTM) derived from airborne laser scanning data and with a resolution of 1, 5 and 10 points m⁻². The group of cultural remains included charcoal kilns, charcoal pits, hollow-roads, various pits, house foundations, tar kilns, grave mounds and pit-falls. The effects on the interpretation success of different types of cultural remains and their physical properties were studied: size, shape and elevation difference showing that the detection success rates varied considerably. The main tendency was that large cultural remains with clear geometrical shape (ovals and circles) and large elevation difference were much more successfully detected and classified compared to the smaller ones, especially those without a clear geometrical shape. The study also showed that it was the identification of the larger structures which profited most from an increased resolution of the DTM, and it was of no help to increase resolution in order to improve the identification of the irregularly shaped cultural remains.     

Detecting Cultural Remains in Boreal Forests in Sweden Using Airborne Laser Scanning Data of Different Resolutions

ABSTRACT

Airborne laser scanning (ALS) is increasingly being used to detect cultural remains in forest landscapes. Boreal forests are challenging, however, since most ancient land use was carried out without major permanent ground disturbances. If this challenge can be met, there is a large potential for surveys through existing nation-wide laser-scanning programs, although their resolutions tend to be low. In this study, we compare the performance of low-resolution (LR) and high-resolution (HR) ALS data in the Krycklan catchment in northern Sweden, an area where ancient land use was small-scale and diverse. About three times as many cultural remains were detected in the HR data set, but the LR set was satisfactory for distinct structures. We analyze how LR data sets can be enhanced at ground-point classification and terrain-model generation and conclude that ALS data have a large potential for the detection and protection of cultural remains in the boreal forest.     

Migration and Viking Dublin: paleomobility and paleodiet through isotopic analyses

During the early medieval period in Ireland, Dublin was established as the largest Viking settlement on the island in the ninth century AD. A previous biodistance study has suggested that the population of the town consisted of a polyethnic amalgam of immigrant and indigenous. In this study, we use biogeochemistry to investigate paleomobility and paleodiet in archeological human remains from the ninth to eleventh century levels at the sites at Fishamble Street II (National Museum of Ireland excavation number E172), Fishamble Street III (E190) and John’s Lane (E173), as well as twelfth-century remains from Wood Quay (E132). Through radiogenic strontium isotope, stable oxygen, carbon, and nitrogen isotope, and elemental concentration analyses, we investigate the origins of the individuals who lived and died in early and late Viking Dublin. Mean archaeological human enamel and bone isotope values from Dublin are ⁸⁷Sr/⁸⁶Sr = 0.70975 ± 0.00139 (2σ, n = 22), δ¹³C_{carbonate(V-PDB)} = −14.8‰ ± 0.8‰ (1σ, n = 12), and δ¹⁸O_{carbonate(V-PDB)} = −7.2‰ ± 1.0‰ (1σ, n = 12). Archaeological human bone samples exhibit mean δ¹³C_{collagen(V-PDB)} = −20.8‰ ± 0.5‰ (1σ, n = 12) and mean δ¹⁵N_{collagen(AIR)} = +10.0‰ ± 1.7‰ (1σ, n = 12). Comparing these data with archaeological faunal data from Dublin and published data from northern Europe, we argue that there are no clear immigrants from other parts of the North Atlantic, although there is one clear outlier in both origins and diet. Overall, the relative homogeneity in both paleomobility and paleodiet may support models of acculturation in Viking Dublin, rather than a high number of first-generation immigrants or continued migration from Scandinavia.     

Provisioning Inka feasts at Tiwanaku, Bolivia: the geographic origins of camelids in the Pumapunku complex

While political integration can be achieved by many means, here we focus on the use of feasting and statecraft in the Inka Empire of the Andean Late Horizon (c. AD 1400-1532) in South America. In order to examine Inka political integration of the Lake Titicaca Basin of Bolivia, we examine paleomobility and paleodiet through radiogenic strontium and stable oxygen and carbon isotope data in archaeological camelid remains from the site of Tiwanaku. Mean radiogenic strontium isotope values from all archaeological camelid enamel and bone samples is ⁸⁷Sr/⁸⁶Sr = 0.70998 ± 0.00179 (1σ, n = 48), mean stable oxygen isotope values from a sub-set of archaeological camelid enamel and bone samples is δ¹⁸O_{carbonate (VPDB)} = −10.0‰ ± 2.6‰ (1σ, n = 18) and mean stable carbon isotope values from a sub-set of archaeological camelid enamel and bone samples is δ¹³C_{carbonate (VPDB)} = −9.0‰ ± 1.7‰ (1σ, n = 18). While many camelids consumed in these feasting events were likely local to the Lake Titicaca Basin, others came from a variety of different geologic zones, elucidating our understanding of Inka statecraft and the role of feasting in political integration in empires in the past.     

Dietary adaptation during the Longshan period in China: stable isotope analyses at Liangchengzhen (southeastern Shandong)

Rice and millet were staple crops at Liangchengzhen, a late Neolithic Longshan site in Shandong, China, but the degree of dietary variation is not known. This study uses stable isotope analysis of human and faunal skeletal remains to quantitatively address the importance of these crops as well as terrestrial domesticates and aquatic resources in the diet at Liangchengzhen. Although no collagen could be extracted from the poorly preserved human bones, the δ¹³C stable isotope results for 2 apatite sample and 16 tooth enamel samples averaged −9.8‰ suggesting that diet was based on foods averaging from −24‰ to −18‰, with millet and millet-fed animals comprising at most approximately 25–30% of the diet. Pig faunal δ¹³C isotope values suggested that during the earlier Longshan period pigs were fed mainly millet with more C₃ foods such as rice included by the later Longshan period. Solid ceramic residues from two guan jar sherds produced δ¹³C values averaging −18‰ and δ¹⁵N values averaging +16‰, suggesting both vessels contained fish. The results of the study indicate that by the Longshan period, people in southeastern Shandong no longer relied as heavily on millet and that the agricultural crop of rice had increased in importance at Liangchengzhen. Unfortunately, without human collagen samples to provide nitrogen isotope results, we cannot estimate the relative contribution of aquatic and terrestrial protein to the diet of people at Liangchengzhen. In general, however, the pattern of a diverse agricultural system on the basis of the macrobotanical remains from Liangchengzhen is supported by the isotopic results.     

Human–environment interactions in medieval Poland: a perspective from the analysis of faunal stable isotope ratios

Stable isotope analyses of faunal remains provide valuable information about human–environment interactions in the past, including insights into past animal husbandry and land management strategies. Here, we report stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope values of collagen and carbonate from archaeological fauna from Ka?dus, a medieval settlement in North-Central Poland, to better understand human–environment interactions during a period of increasing urbanism and marketization. Wild and domestic animals can be separated on the basis of their isotopic values. The mean δ¹⁵N value for 12 domesticated animals is 7.6 ± 1.2‰ and for 5 wild animals is 4.3 ± 0.5‰ (p = 0.002). The mean collagen δ¹³C value for domesticated animals is −20.6 ± 1.1‰ and for wild animals is −22.0 ± 0.5‰ (p = 0.004). The mean carbonate δ¹³C value for domesticated animals is −13.14 ± 1.3‰ and for wild animals is −14.14 ± 0.9‰ (p = 0.034). The “canopy effect” and anthropogenic effects that alter stable isotope ratios of plants (manuring, swidden agriculture and ploughing) are discussed in relation to these differences. Fish are isotopically variable, which suggests broad-spectrum fishing strategies and/or trade, and increases our awareness of the difficulties in interpreting human paleodiet when freshwater fish were on the menu.     

Stable isotopic analysis of human and faunal remains from the Incipient Chulmun (Neolithic) shell midden site of Ando Island,Korea

We analyzed stable carbon and nitrogen isotope ratios of human and animal remains from the Ando shell midden, South Korea. The Ando site is a rare Incipient Chulmun (Neolithic) site (ca. 6000–5000 BC), which contains well-preserved human and animal bones in shell mounds. The stable isotope results for humans (average δ¹³C = −13.5 ± 0.5‰ and δ¹⁵N = 15.2 ± 0.5‰) indicate that Ando people in the Incipient Chulmun period strongly depended on marine resources. There were no isotopic differences between humans of different sex and age at this site. We compared our data with other previous published isotopic data from the Chulmun sites and found that the Ando people had similar isotope values to the southern Chulmun people (Tongsamdong and Daepo), but different isotopic ratios than the western Chulmun people (Daejukri and Konamri). These results indicate that marine foods were the main food resources in the southern coastal regions, but not in the western coastal regions in Chulmun Korea.     

Man and woolly mammoth at the Kraków Spadzista Street (B) – taphonomy of the site

Excavations at the Kraków Spadzista Street (B) site, Poland were conducted over 16 years in which approximately 150 m² of the site was studied. The radiocarbon dates clustered around 23–24 ky BP, placing the site in the Gravettian cultural complex. In this paper, results from a detailed taphonomic study of faunal remains are discussed in conjunction with previous analyses of stone artefacts and site structure in order to address questions regarding site formation and Gravettian occupation. Up to 1994, ∼9000 bone remains were recovered and it was possible to identify the species and skeletal element for 5860 remains. The site contained bones of seven animal species associated with Pleistocene steppe–tundra habitats. At Kraków Spadzista Street (B), 99% of the faunal remains belong to the woolly mammoth, with other large mammal taxa represented by only isolated bones and teeth. This site yielded the largest number of mammoth bones and most individuals (MNI = 86) ever found in one place in Poland. Kraków Spadzista Street (B) represents a mammoth butchering locality and probably a mammoth hunting site as well. However, it is not yet possible to distinguish the mammoths killed by Gravettian hunters from those that died naturally and then were scavenged by people.     

A rock magnetic record of Pleistocene rainfall variations at the Palaeolithic site of Attirampakkam,Southeastern India

Attirampakkam, an open-air stratified Palaeolithic site in southeastern India is the focus of ongoing studies to investigate the nature of Lower and Middle Palaeolithic occupation in relation to changing Pleistocene environments. The paucity of faunal or palaeobotanical remains at the site required the use of rock magnetic properties (χ_lf, χ_fd, χ_fd %, χ_ARM, SIRM, χ_ARM/SIRM, SIRM/χ_lf, χ_ARM/χ_lf, χ_ARM/χ_fd, S-ratio, HIRM and HIRM/IRM_300mT) as proxies for deciphering the palaeoenvironments and palaeoclimates of the region. Rock magnetic data from stratified deposits within excavated trenches show that the bulk of the sedimentation was intermittent with no subaerial exposure of sediments for long periods, and points to fluctuating wetter and drier climates associated with the Acheulian to Late Middle Palaeolithic levels. There is a general trend towards a wetter climate from Layer 5 upwards. Layer 7 offers evidence of bacterial magnetite (χ_ARM/χ_lf > 40 and χ_ARM/χ_fd > 1000), suggesting the onset of micro-anaerobic environments as a result of floods. Greigite is indicated in Layer 5 (SIRM/χ_lf > 30 × 10³ A m^?1), which indicates a reducing environment. Layer 2 represents an arid climate (higher proportion of haematite and a coarser magnetic grain size). Layer 1a again is indicative of an arid climate (presence of calcrete, magnetically “hard” minerals and coarser magnetic grain size). Results indicate that the southeast coast of India experienced a mostly dry tropical climate during the Pleistocene. The detected occurrence of a few notably more arid intervals did not disrupt occupation of the site by successive groups of hominins.     

Zooarchaeological perspectives on the Chinese Early and Late Paleolithic from the Ma’anshan site (Guizhou,South China)

Ma’anshan is a Paleolithic cave site in the Guizhou province of southern China. The total area of the excavations is ca. 48 m², and the cultural deposit is divided into two layers representing the Chinese Late Paleolithic and the later part of the Early Paleolithic. The upper layer dates between 19,295  BP and 31,155 BP by AMS technique, and the lower layer is dated to around 53,000 BP by U-Series technique. Thousands of bone fragments were recovered from the two layers. Species abundance, bone surface modifications, skeletal element representation, and mortality patterns were studied in an investigation of assemblage formation history and patterns of prey selection and meat consumption by hominins. Interpretations of the faunal data are strengthened by reference to experimental studies and ethnoarchaeological data. The zooarchaeological data indicate that the later occupants of Ma’anshan Cave hunted mainly medium and small game animals, while the earlier occupants tended to prey upon larger animals. In the later period, hominins also made fuller use of the carcasses. The breadth of the meat diet increased with time, due to the inclusion of quick small animals (bamboo rats and birds) in the later period. The differences between the two faunal assemblages are consistent with the chronologic boundary currently drawn between the Chinese Early and Late Paleolithic ca. 30–27 ka BP, based on technological and other evidence.     

Near-surface imaging of a buried foundation in the Western Desert,Egypt, using space-borne and ground penetrating radar

ALOS/PALSAR L-band full polarimetric data have been used to detect and characterize a well-defined geometric target hidden under sand deposits in the Western Desert of Egypt. This buried object is rectangular in shape with very straight boundaries and encloses an area of 500 m². Inside the rectangular area there is a perfectly circular feature of approximately 53 m² area. High and medium resolution optical satellite images (WorldView-2, ALOS/AVNIR-2) and field investigation confirm that the topography of the study area is relatively flat and completely covered by dry sand. The electromagnetic wave scattering behavior of the detected buried object was measured by decomposing the scattering matrix of ALOS/PALSAR full polarimetric data into Entropy-Alpha (H/α) and Freeman and Durden decompositions. Furthermore, a combination of unsupervised classification algorithms and Constant False-Alarm Rate (CFAR) edge detection was performed on the polarimetric data which revealed a well-defined geometric shape with straight borders. These borders have high α angles with low entropy, which physically means double bounce scattering mechanism with low randomness and is very similar to that of an urban scatterer, indicating that the detected target might be a buried wall-like foundation of a man-made structure. Moreover, 28 ground penetrating radar (GPR) profiles were acquired using a 270 MHz shielded antenna to validate the satellite radar remote sensing results. The GPR profiles reveal very clear hyperbolic shapes at 1.5 m depth with one of them showing a large hyperbolic shape-like structure, which might indicate the presence of a buried foundation. Thus the site might have significance to archaeological studies of the Western Desert, particularly previous human occupation and the sources of water for such habitation.     

Multi-resolution morpho-bathymetric survey results at the Pozzuoli–Baia underwater archaeological site (Naples,Italy)

Here we show the results of two morpho-bathymetric surveys carried out on the underwater archaeological area of the Pozzuoli Bay (Naples, Italy). Such area is enclosed in the Campi Flegrei active volcanic complex, where bradyseism and recent volcanic activity strongly influenced the coastline shape over the last 2 Ka. The Digital Elevation Model (DTM) of the seafloor allowed to draw the main archaeological features of the Bay, that also include the military complex of Portus Iulius and the ancient thermal and villa complex of Baianus Lacus. The extraction of archaeological features from DTM was achieved thanks to the use of a GIS-derived tool based on the profile curvature algorithm. Thus, the Villa dei Pisoni (1st Century B.C.) and the thermal complex of Secca delle Fumose were investigated by using ultra-high resolution DTMs, that show the presence of Roman pilae for the protection of coastal buildings and also the inner planimetry of the Villa dei Pisoni complex. The observation of coastal engineering structures give also the opportunity to formulate some hypothesis on the evolution of morphology and of the sedimentary pattern of the area, while depth of archaeological remains allowed a rough computation of the rate of subsidence, resulted to be 2.55 (±0.5) mm/y for the Eastern sector (Pozzuoli) and 2.90 (±0.5) mm/y for the western (Baia).     

Human subsistence strategy at Liuzhuang site,Henan, China during the proto-Shang culture (∼2000–1600 BC) by stable isotopic analysis

Since the discovery of the proto-Shang culture, created mostly by ancestors of the Shang clan from the late Neolithic Age to the early Shang period (∼2000–1600 BC), the subsistence strategy and lifestyle of humans in China during their movement southwards have been a great focus. Chinese literature and archaeological findings suggest that the proto-Shang societies were composed of different cultural groups and had various subsistence strategies. For example, at the Liuzhuang site, three types of burials, i.e., stone coffin, wooden coffin and earthen shaft-pit, are found. The wooden coffin and earthen shaft-pit burials had been adopted locally in the Central Plains since the Neolithic Age while the stone coffin burials were usually used by people living in Northeast China and had never been found in the Central Plains before. In this study, stable carbon and nitrogen isotopic analyses were performed on human bones from the Liuzhuang site and animal bones from Zhangdeng site in Henan province, China to determine whether different social groups had various accesses to food resources and whether their dietary difference was related to inequality in social status. Humans have mean δ¹³C and δ¹⁵N values of −7.6 ± 0.6‰ and 9.6 ± 1.0‰ (n = 19) respectively, which strongly indicates that humans rely primarily on C₄-based food. The main contribution of C₄-based food in their diet is from millet agriculture or animals that consume millet by-products. The isotopic spacing of carbon and nitrogen isotopic values between pigs and humans, between dogs and humans, and between cattle and humans, all imply that these animals were the main meat resources for humans. Surprisingly, the δ¹³C values and δ¹⁵N values of humans with different types of burials are quite close, indicating that they had equal access to food resources. This result suggests that the proto-Shang humans had adapted to the local subsistence strategy, and the local cultural factors in the Central Plains were very well integrated into proto-Shang culture.     

Soil and organic carbon losses from varying land uses: a global meta‐analysis

Soil (SL) and organic carbon losses (SOC_L) have increased with human population and climate change linked extreme events. While SL and SOC_L are clearly associated with surface run‐off, the impact of land use is still not well understood. This article elucidates the effects of land use, surface cover, and other environmental factors on SL and SOC_L using data from 55 published studies. The overall SL (12.34 ± 2.25 Mg ha^?1 year^?1) and SOC_L (21.80 ± 0.91 g C m^?2 year^?1) suggests substantial losses of the fertile topsoil globally. Grasslands (4.19 ± 1.22 Mg ha^?1 year^?1) had 77%, 66%, and 41% lower SL than orchards, croplands, and forests, respectively, which is attributable to higher soil organic matter and surface cover. Croplands had the highest SOC_L at 22.78 ± 2.22 g C m^?2 year^?1. Natural vegetation had 98% and 70% lower run‐off and SL than did plant residue mulched plots, indicating its great potential for surface run‐off and soil erosion control. Rainfall and slope were key drivers of soil erosion, while soil surface cover, SOC, and clay content decreased. These findings improve our knowledge on soil and organic carbon losses, which is useful for fostering sustainable management of soils and natural vegetation to enhance ecosystems functionality.     

Roman blue-green bottle glass: chemical–optical analysis and high temperature viscosity modelling

Chemical analysis, optical absorption spectroscopy and mathematical modelling of high temperature viscosity have been carried out on five 1st–2nd century AD Roman blue-green bottle glass fragments from Coppergate, York. Modelled viscosities indicate remarkable consistency within the sample set studied and support the suggestion that temperatures of ∼1000–1150 °C were required to remelt these glasses and to provide suitable viscosities for forming articles. Iron redox ratios (Fe²⁺/ΣFe), analysed SO₃ contents and the absence of characteristic Fe³⁺–S²⁻ amber absorption bands suggest that melting conditions for all studied glasses were mildly, rather than strongly, reducing (estimated pO₂ ≈ 10⁻¹–10⁻⁶ bar). These furnace conditions are consistent with the effects of combustion gases and carbonaceous matter contained in the raw materials.     

Spatial–temporal distribution and geographic context of Neolithic cultural sites in the Hanjiang River Basin,Southern Shaanxi,China

Understanding how to live successfully within our environment is among the most pressing challenges facing contemporary society. This paper probes the problem based on comparative analysis and discusses the relationship between the spatial–temporal distribution of the Neolithic cultural sites and the geographic context in the Hanjiang River Basin in the south of Shaanxi Province, China. Archaeological studies have identified 175 Neolithic cultural sites in the study area, with a sequence of Laoguantai (¹⁴C age 8–7 ka BP), Yangshao (¹⁴C age 7–5 ka BP) and the late period of the Neolithic Age (¹⁴C age 5–4 ka BP). The total number of archaeological sites, the distribution area and the density all showed an early ascending and later descending trend, but the proportion of the number of archaeological sites in the study area to the corresponding value of the entire Shaanxi Province declined sharply. Spatially, these sites were concentrated on the terraces of the Hanjiang River and its main tributaries with an altitude of 400–800 m. Multiple data were integrated to clarify the critical effects of tectonic and geomorphologic conditions on the distribution of the Neolithic sites. Further comparisons revealed the correlation of Holocene climate change and environmental evolution with the Neolithic cultural succession in the study area that ameliorated conditions to generally promote the development of the primitive culture while degeneration coincided with the culture's transition or interruption. The discussion on the origin of the primitive culture and the temporal–spatial distribution corresponding to the regional culture differentiation sheds light on the complex and dynamic human–nature interaction system during the Neolithic Age, thus emphasising the wider field-based investigation and high-resolution reconstruction works of the palaeoclimate and palaeoenvironment in the future.     

Palaeoenvironmental significance of grain-size distribution of river flood deposits: a study of the archaeological sites of the Apengjiang River Drainage,upper Yangtze region,Chongqing, China

The grain-size distribution (based on cumulative probability curves) in the sediments produced by potential palaeofloods at the Shiniusi archaeological site is similar to that of modern flood sediments from the Wujiang River Drainage in the upper Yangtze River. There is an obvious pattern in the curves, with two segments, and the mean grain size (Mz), standard deviations (σ1), skewness, and kurtosis are all similar. Combined with the AMS¹⁴C dating data and the ages judged based on the presence of cultural remains, our data suggests frequent palaeoflood events within the Wujiang River Drainage. We hypothesize the existence of seven high flood possibility layers in the QST4 unit from Shiniusi archaeological site: during the periods of the Ming Dynasty (1368–1644 AD) to the Qing Dynasty (1616–1911 AD), and the end of the Eastern Zhou Dynasty (770 BC–256 BC) to the Shang Dynasty (1600–1100 BC). We also hypothesize ten high flood probability layers in the QST2 unit from Shiniusi archaeological site: during the periods of the Ming Dynasty to the Qing Dynasty, the periods of Song Dynasty (960–1279 AD) to the Yuan Dynasty (1206–1368 AD), as well as during the Han Dynasty (207 BC–220 AD) to the Eastern Zhou Dynasty (770 BC–256 BC). These results are consistent with palaeoflood events inferred from pollen and spore analysis and from historical records in other rivers.     

Stable isotope analysis of human and animal remains from the Late Upper Palaeolithic site of Balma Guilanyà, southeastern Pre-Pyrenees,Spain

Stable isotope analysis of carbon (¹³C/¹²C) and nitrogen (¹⁵N/¹⁴N) was performed on collagen extracted from three human and five herbivore bone and tooth samples from the Late Upper Palaeolithic site of Balma Guilanyà (Catalonian Pre-Pyrenees, Spain). Contextual and palaeoecological data as well as radiocarbon dates indicate that the studied occupation phase took placed during the Bolling/Allerod interstadial (GI-1a event). The human remains were co-mingled without any anatomical association, corresponding to a minimum number of three individuals, and it was not possible to determine if the three analyzed samples are from one or more individuals. The mean isotope values obtained from the human remains are δ¹³C = −19.8‰ and δ¹⁵N = 6.7‰, while those of the large herbivores (red deer and wild goat) were −19.8‰ and 1.7‰ for δ¹³C and δ¹⁵N respectively. This indicates that the main source of protein in the diet of the Balma Guilanyà human(s) came from terrestrial herbivores. There is no zooarchaeological or isotopic evidence for the consumption of freshwater or marine resources at the site, which lies 80 km from the present Mediterranean coast. The low δ¹⁵N values observed in both human and animal samples correspond to a trend reported by other researchers working in northwestern Europe: a significant δ¹⁵N reduction in collagen from bones datable within 20,000–10,000 BP, followed by a rise to present values in the Early Holocene. This phenomenon, generally attributed to climatic and/or pedological processes, had not been previously observed in the Mediterranean region and, until now, was thought to be restricted to northern Europe.     

Elephants and subsistence. Evidence of the human exploitation of extremely large mammal bones from the Middle Palaeolithic site of PRERESA (Madrid, Spain)

The archaeological site at PRERESA (Madrid, Spain) has been dated to 84 ± 5.6 ka by optically stimulated luminescence (OSL) (MIS 5a). An area 255 m² was excavated and 754 lithic pieces were recovered, as well as a large amount of micro and macro vertebrate remains, including proboscidean bones. The aim of this paper is to outline the results of the taphonomic study of these remains. The identification of cut marks on a number of the bones recovered strengthens the theory that the exploitation of extremely large mammals was more than just a marginal practice before the Upper Palaeolithic. Additionally, the identification of green-bone fractures and percussion marks confirm for the first time, that the bone marrow of these taxa was also consumed. Few other cases of this practice have been identified, firstly because obtaining this substance would not be an easy matter, and secondly because similar nutritional needs can also be met by the consumption of brain matter, which is easier to acquire.     

On the elimination of extraneous DNA in fossil human teeth with hypochlorite

Elimination of extraneous DNA in fossil specimens is of paramount importance for the successful isolation and analysis of authentic DNA; this is especially true when the specimens are of human origin. Bones and teeth are commonly decontaminated with bleach containing the powerful oxidising hypochlorite ion. The procedures involve either submersion in or wiping with the chlorine agent. Using the radioactive isotope Cl³⁶ we showed that submersion of fossil teeth in solutions of small ions such as Cl⁻ or hypochlorite, ClO⁻, cause that they migrate right into the pulp. This may lead to the unwanted destruction of authentic DNA. However, using pairs of teeth from the remains of four ancient Europeans (1000–2000 YBP) as well as tooth and hair from an Inuit skull (>300 YBP) we provide evidence that at least some endogenous human fossil DNA survives in powdered pulp/dentin that has been submersed in 2% hypochlorite. Further, we show that powdered pulp/dentin deliberately contaminated with huge amounts of a 414 bp PCR product is effectively decontaminated by suspension in 2% hypochlorite for 5 min. Decontamination of fossil material from teeth may therefore be accomplished by a short direct action of hypochlorite on the powdered specimen rather than less controllable and less efficient external treatments of the whole specimen.