Sickle blade life-history and the transition to agriculture: an early Neolithic case study from Southwest Asia

In Southwest Asia, sickle blades first appear early in the sequence of the transition to agriculture. In the past, detailed qualitative research on silica bearing blade stone tools focus on the characterization of use-wear traces such as polish types and accrual rates. In this paper we approach the study of sickle blades slightly different, choosing to examine tool life-history by developing a method to quantitatively estimate harvesting intensity. The method centers on an experiment of cutting cereal stalks and measuring stone blade edge thickness under a scanning electron microscope as a proxy for cutting time. We end with regressing the experimental results to provide an estimation of how intensively archaeological sickle blades recovered from the site of Dhra’, Jordan were used for harvesting. The results, while preliminary, enable an initial interpretation of sickle blades as important tools with long use-life histories during the early Neolithic in the Southern Levant.     

Tracing sickle blade levels of wear and discard patterns: a new sickle gloss quantification method

Although sickle blades gloss is known to form during the harvest, which is a dynamic process and although it is known to be a type of wear which is the outcome of the repetitive encounters between the sickle blades working edges and plant stems; there is no common way to quantify its intensity. Since there is no consensus over a specific scale that will enable the estimation of the amount of wear, based on the quantification of gloss brightness intensity. In this paper we propose a new measurement system which is based on the projection of laser (He–Ne) beam on glossed sickle blades and an analysis of the reflected images. The results form a relative scale from which it is possible to infer on the sickle blades level of wear, and to create discard models.     

Analysis of ancient mycolic acids by using MALDI TOF MS: response to “Essentials in the use of mycolic acid biomarkers for tuberculosis detection” by Minnikin et al., 2010

The mycobacterial cell wall is consists of several long chain lipid and glycolipid components. Mycolic acids are major and unique fraction of the cell envelope of mycobacteria that include the ancient causative agents of tuberculosis and leprosy. The analytical investigation of these lipid biomarker residues is one of the most promising perspectives in the field of molecular paleopathology. Recently published in this journal, Minnikin et al. present a systematic critique of the MALDI TOF MS analysis for determination of ancient mycolic acids, focusing on our previous paper. In this study, our mass spectrometric investigations by commonly used 2,5 DHB matrix were presented with our comments to the critique authors. On the base of our previous and recent mass spectrometric results we have to realize that the clinical protocols and standards cannot directly be used for the biomolecular paleopathological investigations. The applicability of the recent mycobacterial and clinical results is very limited in the biomolecular archaeology, thus the recent scientific results and protocols should be adapted carefully to bioarchaeological sciences.     

Biomolecular archaeology of ancient tuberculosis: response to “Deficiencies and challenges in the study of ancient tuberculosis DNA” by Wilbur et al. (2009)

It is sixteen years since the first detection of Mycobacterium tuberculosis DNA in archaeological specimens, yet the validity of findings continues to be questioned. Rigorous scientific scrutiny and debate is valuable and has led to a coalescence of procedures and precautions amongst those actively engaged in this work. It is disappointing that these good practices are not recognised by certain scientists whose primary expertise is in the related fields of archaeology, palaeopathology, and eukaryote ancient DNA. There is a danger that by constant repetition, disputable and inadequately justified concerns will assume the status of self-perpetuating myths and misunderstandings. We discuss these issues with reference to a recent article in this journal, in which clear peer-reviewed scientific data were specifically targeted as part of a general critique of the field of the palaeomicrobiology of tuberculosis. We believe we have given sufficient evidence and cogent argument to persuade the unbiased reader that the views in the critique by Wilbur et al. are unjustified.