A DNA test for sex identification in cattle confirms osteometric results

It is of vital importance to be able to sex identify cattle remains to understand the strategies and importance of cattle husbandry in an ancient society. This is usually done from osteoarchaeological assemblages and often relies on measurements of metapodials. The breadth measurement of the distal trochlea is considered an easy way to identify the sex. Bones from males appears to be easily distinguishable from female counterparts, although it has been complicated to find an external control for the morphological results. Here we investigate the reliability of these particular morphometrics for sex identifying cattle bones with molecular genetics. We use a sex discriminating single nucleotide polymorphism in the ZFXY gene and we apply it to DNA from the bones. To keep the fragment size short and suitable for ancient DNA we base the test on a SNP. The test confirms the osteological sex identification in all cases were DNA could be retrieved. This molecular method can also be used when no fragments suitable for osteological sex identification can be found or when the measurements are non-conclusive.     

Molecular and osteometric sexing of cattle metacarpals: a case study from 15th century AD Beja,Portugal

In the course of a zooarchaeological survey of Holocene sites in southern Portugal, a substantial size increase of cattle bones was noted following the Christian reconquista of the 11th–13th centuries AD. A size increase in the course of time within a lineage of domestic livestock is usually considered to represent animal improvement. However several other factors including sex may influence the average size of a sample of mammal bones – cattle exhibit considerable sexual size dimorphism, with bulls being larger than cows. A histogram of the distal widths of a large (n = 44) sample of cattle metacarpals from 15th century Beja (Alentejo, Portugal), revealed a bimodal distribution. It was assumed that the large measurements belonged to males and the small to females. In order to rule out the possibility of a post-Moslem change in the sex ratio of cattle, a sub-sample of 21 cattle metacarpals from Beja was selected and we used genetic markers to identify the sex of the animals to which these metacarpals belonged. The ancient DNA sex of all specimens agreed with the previously assumed sex as determined osteometrically. We conclude that the two nearly separated peaks for the metacarpal distal width measurements do indeed indicate sex. A similar bimodal distribution was obtained from another large but earlier sample of cattle metacarpals from Moslem Alcáçova de Santarém (9th–12th century AD). Although these have not been molecularly sexed and since osteometric sexing has now been validated, we conclude that both small (female) and large (male) peaks are smaller than the 15th century ones and that there was an overall size increase or improvement of cattle in this region. Why the Christians improved cattle is unclear, but a selection for larger beeves for meat is one possibility as is the selection of more robust cattle for power. The spread of the quadrangular or chariot plough in Iberia is known to have occurred at this time. We then use the genetically sexed metacarpals to determine which measurements provide reasonable distinction between the sexes. Both the distal width (BFd; as already noted by Svensson et al., 2008; in Swedish medieval cattle) and the width of the lateral condyle (WCL) offer the best distinction. We also used them as a reference ‘collection’ to sex the medieval and post-medieval cattle metacarpals from Launceston Castle in England. This re-visit of the Launceston data corroborates other evidence indicating increased specialisation (milk and veal) in post-medieval cattle husbandry in England.     

First ancient bovine DNA evidence from India: difficult but not impossible

Ancient DNA isolation from the tropical countries has been shown to be very difficult in the past. Here for the first time we have been successful in isolating ancient DNA from Indian cattle samples. We were able to obtain DNA and sequence the partial mitochondrial D-loop in 3 of the 15 bovine fossil samples ranging in age from 2000 BC to 1000 AD, and were able to further identify the most recent sample as being of Bos indicus origin. Our results on ancient DNA extraction from India will encourage other researchers in this field to carry out further studies of ancient DNA from Indian bovine samples. Our results represent the first successful extraction and amplification of bovine ancient DNA from India, and thus may pave the road for a better understanding of demographic and historical processes of cattle domestication that has taken place in this region.     

Raising Practices of Neolithic Livestock Evidenced by Stable Isotope Analysis in the Wei River Valley,North China

Although a patchwork of projects shows a process of agriculture intensification in North China during the Neolithic, the impact of cereal farming on animal husbandry and their mutual interaction remain cloudy. This study reports bone collagen δ¹³C and δ¹⁵N of humans and animals from Wayaogou (ca 6.5–6.0 kyrs bp ) and Dongying (ca 5.9–5.6 kyrs bp , 4.6–4.0 kyrs bp ) to explore temporal trend of livestock raising and particularly the importance of millet fodder to stock raising practices in the Wei River valley, North China. The isotopic evidence overall shows that millet products increased in human and domestic animal diets during the mentioned chronological span. δ¹³C values of pigs and dogs at Dongying are higher than those at Wayaogou, implying that the importance of millet nutrients increased to animal husbandry diachronically. Interestingly, δ¹³C results of domestic cattle of Dongying late phase (−14.1 ± 1.1‰, N = 5) are more enriched than Wayaogou wild Bos (−17.8 ± 0.3‰, N = 3), indicating that millet fodder had taken a significant place in early cattle husbandry. Besides, differences between Bos species of the two periods also imply that δ¹³C values of bone collagen constitute a potential indicator for tracing the origin of cattle husbandry in North China. In addition, domestic sheep at Dongying produced similar isotope data to wild ovicaprid at Wayaogou, suggesting that they possibly had grazed for the most in grassland and therefore experienced a different lifestyle from cattle. Copyright © 2014 John Wiley & Sons, Ltd.     

Iron Age animal husbandry in the wetlands of the western Netherlands

During the Iron Age a subsistence economy based on mixed farming existed in the Dutch wetlands. A model based on subsistence is put forward and tested against the archaeozoological data of 27 sites in order to gain insight into the role of animal husbandry. The model confirms that cattle husbandry was the dominant form of animal husbandry but pigs and sheep were also important at some sites. Although a high number of pig bones were expected, their role is mostly insignificant. An explanation is sought in their lack of secondary products and their competition with humans for the scarce cereals in the wetlands. The mortality profiles for cattle are largely in accordance with the subsistence model. Older cattle are used for traction, milk and herd security and sheep were mainly kept for their meat but also for their milk and wool. Differences in animal husbandry between sites also exist. At two sites more cattle were killed for their meat and at three sites the proportion of older cattle was larger than expected. Although this first test renders the subsistence model viable, it needs to be tested against more sites with larger bone samples.     

河南地区新石器时代生业方式初探

本文通过梳理河南地区动植物考古研究成果,对新石器时代(距今10000年—4000年)河南地区豫北、郑洛和豫西南三个区域的生业方式进行了初步的综合研究和探讨。研究表明,新石器时代中期河南地区是以采集渔猎为主、种植农作物和饲养家畜为辅的生业模式,经过新石器时代晚期的过渡,发展为以种植农作物和饲养家畜为主、采集渔猎为辅的生业模式;新石器时代末期,农作物中新加入了大豆和小麦,家畜中新加入了黄牛和绵羊,多种农作物种植和家畜饲养方式逐渐形成。人类对动植物资源的掌控、文化和资源的交流是生业发展的主因,同时自然环境对生业起到了制约和促进两方面的影响。在古代社会发展与文明进步的进程中,生业起到了不可或缺的重要作用。     

A calf for all seasons? The potential of stable isotope analysis to investigate prehistoric husbandry practices

The Early Bronze Age barrows at Irthlingborough and Gayhurst in central England are notable for the large number of cattle (Bos taurus) remains associated with their human Beaker burials. Previous work using strontium isotope analysis has indicated that most of the cattle analysed, and one aurochs (Bos primigenius), were of local origin [Towers, J., Montgomery, J., Evans, J., Jay, M., Parker Pearson, M., 2010. An investigation of the origins of cattle and aurochs deposited in the Early Bronze Age barrows at Gayhurst and Irthlingborough. Journal of Archaeological Science 37, 508–515.]. In this study, stable isotope analysis of enamel and bone was carried out to investigate whether the mature cattle had experienced similar husbandry practices, climate and environment. Bulk carbon, nitrogen and sulphur isotope analysis of collagen suggested most were consuming similar sources of plant protein from environments probably local to the sites and this was supported by high resolution intra-enamel carbon isotope profiles. Oxygen isotope profiles indicated the aurochs and most of the cattle experienced similar climatic regimes: the only exception being an animal with a non-local strontium isotope ratio. However, a comparison of seasonality profiles of the local animals using estimated tooth formation times showed that there was no consistency in season of birth: the animals appeared to have been born throughout the year. Cattle can breed throughout the year but it requires considerable human effort and intervention to successfully overwinter young stock; it is therefore unlikely to have been carried out without good reason and benefit if winters were harsh. One reason is to ensure a continuous supply of milk. Measuring oxygen isotope profiles to identify year-round calving may thus be a potential indicator of dairying economies.     

The late adoption of cattle and pig husbandry in Neolithic Central Turkey

This paper summarizes and interprets zooarchaeological evidence for cattle and pig domestication in Neolithic Central Anatolia. Biometric and demographic data indicate that domestic cattle first appear in the region in the late seventh millennium cal BC while domestic pigs are not evident until the mid fifth millennium. This places the origins of cattle and pig husbandry in Central Anatolia considerably later than in neighboring regions. Reasons for this delay in the spread of productive animal husbandry practices are explored.     

汉晋时期河西走廊张掖地区的开发研究——以黑水国汉代墓葬为例

黑水国汉代墓葬出土的农具、动物骨骼、农作物遗存和人骨为人们了解河西走廊张掖地区汉晋时期生业经济和农业发展等问题提供了重要证据。通过动物骨骼、农作物遗存鉴定结果和人骨碳氮同位素检测结果,可以揭示汉晋时期黑水国人群的食物结构和生业模式特点。墓葬出土的农作物有粟、黍、稻,随葬动物有鸡、猪、绵羊、黄牛、狗、马,人骨骨胶原碳稳定同位素主要呈现C₃和C₄混合信号,部分呈现C₃信号和C₄信号,氮稳定同位素值相较牛羊等家养食草动物较高。综合研究说明汉晋时期河西走廊形成麦类、粟黍和豆类的混合农业模式,所以黑水国汉代先民摄取的粮食作物来源呈多样化。同时,河西走廊畜牧业发达,使当时人群摄取了大量的肉食资源,导致蛋白质摄入量较高。     

明清时期畜养业结构的变化

本文主要根据历史文献资料的相关记载对明清时期畜养业结构的变化进行了初步探讨。总体而言 ,无论是在牧区还是在农区 ,牧马业的地位都呈下降趋势 ,而牛、羊、猪及各种家禽的饲养则逐渐走向繁荣 ,其中牛、羊的放牧在北方较为普遍 ,猪及各种家禽的饲养则以南方为盛。明清时期畜养业结构的这种变化与当时社会政治经济形势的变化有密切的关系 ,是各种因素综合作用的结果 ,其中明清王朝立国形势和养马政策的变化以及市场需求的变化和各地贸易联系的加强是最主要的影响因素     

Long and Short‐distance Transhumance in Ancient Greece: The Case of Arkadia

We argue for short‐distance transhumance as a system of livestock mobility in ancient Arkadia, as well as in many other parts of ancient Greece. This model is a sustainable system of animal husbandry that resolves the technical and political issues posed by long‐distance transhumance and provides a historical interpretation of ancient Arkadian economies using a household herding model. Moreover, it helps us to integrate economy and socio‐political identity in mountainous areas. For this reason, we argue that it is necessary to revamp the traditional model for understanding ancient Greek husbandry, which is based on the opposition between astu and chora. This model has contributed greatly to fostering an image of ancient Arkadia as a territory especially dedicated to pastoralism, with the possible presence of long‐distance transhumance. However, archaeological, environmental and historical studies call into question the traditional models and point to other interpretations.     

Stable hydrogen isotopes of bone collagen in palaeodietary and palaeoenvironmental reconstruction

The stable hydrogen isotope ratios (δD) of bone collagen in archaeological human and animal samples demonstrate a trophic level effect, with increasing δD from herbivores to omnivores to humans, in steps of 10–30‰. In addition the archaeological sites studied (Yarnton, Eton Rowing Lake, Danebury Environs–Suddern Farm, and Windmill Hill in the UK, Balatonszárszó in Hungary, and Huari in Peru) demonstrate geographical variation in δD. The detection of manuring in prehistory by comparison of δ¹⁵N to δD values in humans and a local herbivore (cattle) is also considered. This work is the first to measure δD in a large number and range of archaeological samples, with several animal species and humans. It demonstrates unequivocally that δD is different between species in ancient material, increasing from herbivores to omnivores to carnivores.     

Remains of fly puparia as indicators of Neolithic cattle farming

Abstract

Remains of invertebrates were examined from four Final Neolithic settlements of the northern Federsee region in Upper Swabia. High concentrations of fly puparia (Diptera, Sepsidae, Sphaeroceridae, Muscidae) indicate cattle husbandry within the settlements.     

Ancient molecules and human prehistory

Abstract

DNA and other molecules found in ancient remains are yielding new information about the origins, spread, interaction, and culture of early humans. Molecules of animal fats preserved in archaeological pottery have shown that in medieval societies people ate non-ruminant animals, such as pigs, but burned tallow from ruminants, such as sheep and cattle. Geochemical analysis of bitumen in the Middle East has documented ancient trade routes. Studies of molecular genetic diversity have shed light on when and how cattle were domesticated. Analysis of human hair from remains up to 5200 years old has revealed the diets of those ancient people. DNA recovered from other remains has also provided evidence for theories about the origins and spread of agriculture, and human migration into the Pacific.     

Isotopic and zooarchaeological investigation of later medieval and post-medieval cattle husbandry at Dudley Castle,West Midlands

Abstract

Zooarchaeological analysis of a substantial assemblage of animal bones excavated from Dudley Castle, West Midlands, revealed two significant changes in cattle management in the late medieval and post-medieval periods: a statistically significant increase in size in the later 14th century; and a gradual reduction in slaughter age. To explore whether these altered husbandry practices were accompanied by modifications to the diet and environment of these animals, a targeted study of carbon and nitrogen stable isotope values from 135 cattle bones was conducted. This analysis revealed that δ¹³C and δ¹⁵N values were broadly consistent between the mid-13th and mid-18th centuries. Only two statistically significant diachronic variations were identified: a temporary decrease in δ¹³C values in phase 7 (1397–1533); and lower variation in δ¹³C values in phase 9 (1647–1750). A number of explanations for these changes are offered. While the zooarchaeologically attested shifts in cattle husbandry were not accompanied by isotopically detectable changes to diet and environment, the analysis of stable isotopes highlighted other changes that were not detected using traditional zooarchaeological analysis. The complimentary benefits of zooarchaeological and isotopic approaches are extolled.     

Diet of aurochs and early cattle in southern Scandinavia: evidence from 15N and 13C stable isotopes

Stable carbon and nitrogen isotope ratios from ¹⁴C dated bones of early Atlantic aurochs (Bos primigenius Bojanus) and late Atlantic first domestic cattle (Bos taurus Linnaeus) in eastern Denmark and southern Sweden are significantly different and provide information on the origin and feeding strategies of the two species.Radiocarbon dates generally divide the bone material of aurochs and domestic cattle in three groups: aurochs older than 4000 cal yr BP, an older group of domestic cattle around 4000 cal yr BP, and a younger, less well-defined group of domestic cattle starting at around 3500 cal yr BP. The older domestic cattle are represented mainly by fragmentary bones left over from meals, and deposited in lakes at the vicinity of the settlement areas. Bones of the younger domestic cattle group occur both as settlement debris and as single articulated skeletons in bogs, commonly in association with different types of clay pots. The latter type of finds suggests that sacrifice of domestic cattle began at this time. The dating of the early domestic cattle further indicates that they were contemporaneous with or slightly younger than the elm decline, which occurred shortly after 4000 cal yr BC on the Danish island of Sjælland. Our results indicate a sudden rapid introduction of domestic cattle into Denmark, heralding the introduction of agriculture and there is no evidence for leaf foddering or domestication of aurochs. A combination of several natural events may have created the necessary open land, providing the grazing areas for the imported cattle.     

Ancient Humped Cattle in Africa: A View from the Chad Basin

The origins of ancient and modern African cattle are still a matter of much debate among researchers. Part of the dispute involves the question of the appearance in Africa, from the second millennium BC onwards, of cattle carrying a distinguishing morphological feature present in most of the modern sub-Saharan breeds: The hump. This paper addresses the issue of the origins of the African humped cattle. After reviewing the current hypothesis on their origins, the status and significance of old and new archaeological and osteological evidence from the Chad Basin are presented and critically discussed. Mainly based on the cultural context of the archaeological figurative evidence available in the remaining continent, a case for the foreign ancestry of the ancient African humped cattle is made, and a perspective for future research in the topic is provided.     

Cattle mobility in prehistoric Britain: strontium isotope analysis of cattle teeth from Durrington Walls (Wiltshire,Britain)

An important role has been envisaged for cattle during the Neolithic period in Britain based on their prominence within the faunal assemblages of the period as a whole. The relative ease with which cattle can be moved over long distances and the requirement to provide ample pastureland leads almost inescapably to the consideration of prehistoric cattle movement. This paper presents the results of an investigation into the mobility of Late Neolithic cattle at the well-known site of Durrington Walls, Wiltshire. ⁸⁷Sr/⁸⁶Sr values from cattle (Bos taurus) teeth were compared to local vegetation samples, well established values from archaeological material and to known geological conditions in order to determine whether individual animals were raised in areas with similar geological conditions as those found at the site (i.e. chalkland), and therefore whether the animals were of allochthonous or autochthonous origin. In total, 13 mandibular molars from Durrington Walls were analysed. Two of the animals included in the study were certainly raised under conditions similar to those found in the vicinity of Durrington Walls, but the other 11 provided signatures so distinct from that found locally that they could not have been raised on chalkland. From the results it is suggested that cattle were brought to the site from a variety of grazing areas in different parts of Britain. The implication of these findings is that the movement of cattle was undertaken during the Late Neolithic, and that in a number of cases substantial distances must have been traversed in order for animals to reach the site. In addition, the study provided valuable information for the interpretation of the site, which attracted people from a variety of regions, probably for ceremonial reasons.     

DNA analysis in charred grains of naked wheat from several archaeological sites in Spain

In the present work we attempt to recover endogenous ancient DNA from cereal grains preserved under different conditions: charred, partially charred and waterlogged. A total of 126 grains from naked wheat and 18 from barley from different sites on the Eastern Iberian Peninsula ranging from the beginning of agriculture in the region to the turn of the Common Era, were studied. Two different extraction protocols were used, a standard phenol–chloroform method and a silica-based DNA extraction procedure implemented for artificially charred seeds. Amplifications were directed to three markers: the large subunit of ribulose 1,5 biphosphate carboxylase (rbcL) and the microsatellite WCT12 in the chloroplast genome and the x and y subunits of the high molecular weight glutenin gene (Glu-1) in the nucleus. The first two were used to assess the preservation status of the samples, while with the third we tried to identify the wheat grains at species level. It was possible to obtain eleven positive amplifications in 8 partially charred seeds but only two amplifications of the Glu-1 gene from a single sample of the Early Bronze age were genome-specific. Different contamination sources were identified and reported. Cloning and alignment of sequenced clones showed a correspondence of the amplified fragment to modern wheat D genome haplotypes. This result suggests that the sample corresponds to hexaploid wheat (Triticum aestivum L.), thus being the first ancient DNA evidence to date for the cultivation of hexaploid wheat in the prehistoric agriculture of the Iberian Peninsula. Moreover, obtained results highlight contamination problems associated to the study of ancient archaeobotanical charred seeds suggest that the combination of a silica-based extraction method together with the amplification of specific targets is a good strategy for recovering endogenous ancient DNA from this kind of material.     

Isotopic Evidence for Camelid Husbandry During the Formative Period at the Pacopampa Site,Peru

ABSTRACT

Camelids were domesticated in the Andean highlands, such as in the puna habitat, and dispersed into lowland areas and the northern Central Andes. As camelids domesticated in a particular region would have had a greater economic benefit than visiting- or hunted wild camelids, it is important to reconstruct the dispersal of camelid husbandry from its initial site throughout the ancient Andean civilisation. We carried out multi-isotope analyses of animal remains recovered from the Pacopampa site to investigate the nature of camelid pastoralism and utilisation. Strontium and oxygen isotope ratios from tooth enamel suggested that camelids in the early Late Formative Period (800–500 BC) were born near the site and remained in the same habitat for up to three years. Although corresponding data for the Middle Formative Period (1200–800 BC) were not available, carbon and nitrogen isotope ratios were statistically different from those of the Late Formative Period, supporting the possibility that the camelids inhabited the highland plateau like puna. It is inferred that in the northern highlands camelids were initially rare and regarded as either tribute or ritual animals, or they were used as pack animals. Camelid husbandry using maize as fodder began during the Late Formative Period at Pacopampa.