Evidence for early viticulture in China: proof of a grapevine (Vitis vinifera L., Vitaceae) in the Yanghai Tombs,Xinjiang

A stem was discovered in the Yanghai Tombs, Turpan District in Xinjiang, China. Anatomical features showed it to be of grape (Vitis vinifera L.). Radiocarbon dating indicates it to be nearly 2300 years old, which would suggest that there was grape cultivation at least from that time. To date, this is the earliest physical evidence of V. vinifera cultivation in China.     

Plant microfossil analysis reveals disturbed forest and a mixed-crop,dryland production system at Te Niu,Easter Island

Plant microfossil analysis was carried out on 12 soil samples from a variety of landscape features at three locations (100–250 m a.s.l.) along a 950 m transect at Te Niu, Easter Island. Pollen and phytolith assemblages were dominated by palms and ground ferns, and suggested disturbed forest. We identified pollen of bottle gourd (Lagenaria siceraria) and starch grains of the common yam (Dioscorea alata), sweet potato (Ipomoea batatas) and taro (Colocasia esculenta). The data suggest a mixed-crop, dryland production system at Te Niu dominated by yam and sweet potato, and supplemented by taro and bottle gourd. The data provide direct evidence of crop type and range, supporting the indirect evidence (topographic and landscape features, field and historical research, comparisons with elsewhere in the Pacific) that much of the rock covered landscape of Easter Island was used for intensive horticulture.     

The origin of the ancient Egyptian drink Shedeh revealed using LC/MS/MS

Ancient Egyptians were buried with the most precious food and drink as sustenance for their afterlife. One of these was Shedeh, the most valued and appreciated beverage in ancient Egypt. The botanic origin of Shedeh remains unclear as no mention of its raw material has survived. Some scholars have proposed that Shedeh was a pomegranate wine, while others, a grape wine. Presented here is the first ever analytical evidence of Shedeh's origin through the analysis of a sample of a residue from an extraordinarily well preserved Shedeh amphora from King Tutankhamun's collection. The previously developed LC/MS/MS wine markers method for archaeological samples was used and our results reveal Shedeh had a red grape origin.     

A short note on banana (Musa) phytoliths in Lapita,immediately post-Lapita and modern period archaeological deposits from Vanuatu

We present results of phytolith analysis of deposits from three prehistoric settlement sites in Vanuatu: the small islands of Vao and Uripiv off the north east coast of the main island of Malekula and at Mafilau on the west coast of Epi Island. Samples from Vao Island coarsely encompass the period from Lapita times (c. 3000 BP) to the present. The data from this site suggest deforestation by people, with a decrease in palms and increase in grasses. The data from the three sites show evidence of an introduced cultigen during different prehistoric periods. Banana (Musa) phytoliths were found in one recent Vao layer (500 BP-present) and in Mafilau layers, the latter representing the immediate post-Lapita period (c. 2800–2500 BP). This phytolith type was also found at Uripiv in Lapita layers (c. 3000–2700 BP). Banana phytoliths are diagnostic so their presence in archaeological deposits in Remote Oceania, outside their natural distribution, provides secure evidence of cultivation. This demonstrates the potential of this line of evidence for identifying both the cultigens transported by early colonists and subsequent plant introductions. The data provide the first direct evidence of banana cultivation at a Lapita site in Remote Oceania and support the contention that horticulture was an integral part of the Lapita Cultural Complex.     

Sweet potato (Ipomoea batatas) and banana (Musa sp.) microfossils in deposits from the Kona Field System,Island of Hawaii

Analysis of plant microfossils (pollen, phytoliths, starch grains and xylem cells) was carried out on 12 soil samples from a variety of pre-contact archaeological features in South Kona within a portion of what has been termed the Kona Field System, on the Island of Hawaii. The oldest radiocarbon ages of the sampled deposits are 1300–1625 AD and 1310–1470 AD. The pollen and phytolith evidence suggests a change from more to less trees and shrubs in the area as a result of human activity. We found phytoliths of banana (Musa sp.) leaves in most and starch grains and xylem cells of tuberous roots of c.f. sweet potato (Ipomoea batatas) in all of the samples, suggesting that the sampled features were associated with these crops and that both crops were cultivated intensively within the study area. Higher concentrations (volumetric) of starch and xylem in samples from older deposits suggest that cultivation was more intensive then. The apparent absence of starch and xylem remains of other tuberous crops archaeologically identified elsewhere in Polynesia suggests that tuberous cropping within the study area was mono-specific.     

Chemical analysis of 17th century Millefiori glasses excavated in the Monastery of Sta. Clara-a-Velha,Portugal: comparison with Venetian and façon-de-Venise production

A set of ten Millefiori glass fragments dating from the 17th century, originated from archaeological excavations carried out at the Monastery of Sta. Clara-a-Velha (Coimbra, Portugal), were characterized by X-ray electron probe microanalysis (EPMA), Raman microscopy and UV–Visible absorption spectroscopy. All glasses are of soda-lime-silica type. The use of coastal plant ash is suggested by the relatively high content of MgO, K₂O and P₂O₅, as well as by the presence of chlorine. Tin oxide or calcium antimonate were the opacifiers used in the opaque glasses, cobalt in the blue glasses, copper in the turquoise glasses, iron in the yellow and greenish glasses, and iron and copper were found in the opaque red and aventurine glasses. Based on the concentrations of alumina and silica four different sources of silica were identified, allowing the classification of the glasses into the following compositional groups: low alumina (<2 wt%), which includes a sub-group of cristallo samples with SiO₂ > 70 wt%, medium alumina (2–3 wt%), high alumina (3–6 wt%) and very high alumina (>6 wt%). Comparison with genuine Venetian and façon-de-Venise compositions showed that two fragments are of Venetian production, one of Venetian or Spanish production and the remaining are of unknown provenance. In two fragments the glass of the decoration is probably Venetian or Spanish but the glass used in the body is also of unknown provenance.     

Ancient DNA analysis of desiccated wheat grains excavated from a Bronze Age cemetery in Xinjiang

Wheat has been one of the most important crop in Eurasia since the Neolithic period. Understanding the spread of wheat cultivation is crucial to understanding the spread of agriculture as a whole and the interactions between prehistoric populations across the Eurasian continent. However, the routes by which wheat cultivation spread eastwards have been poorly understood to date, due to the scarcity of plant remains recovered from archaeological sites. Desiccated wheat grains excavated from the Xiaohe cemetery in Xinjiang, and dated to the early Bronze Age, show excellent DNA preservation. Here we present an ancient DNA (aDNA) analysis of wheat (Triticum sp.) grains excavated from Xiaohe and provide the first definitive evidence for bread wheat in China during the Bronze Age. The nuclear ribosomal DNA internal transcribed spacer regions (ITS1 and ITS2) and the intergenic spacer region (IGS) were amplified. The IGS region within the D genome of wheat has a 71 bp insertion that is absent from corresponding regions in the A and B genomes. The results showed that the Xiaohe wheat showed most sequence similarity to hexaploid bread wheat (Triticum aestivum), including the characteristic insertion into the D genome. The presence of bread wheat at the Xiaohe cemetery is discussed in relation to it having spread into Xinjiang by the Bronze Age, providing new insight into the origins of bread wheat in East Asia.     

New investigations into the Uluburun resin cargo

Resin found within Canaanite amphorae from the Late Bronze Age shipwreck discovered off the coast of southwest Turkey at Uluburun has previously been identified as Pistacia sp. Although evidence from Egypt suggests that this resin was in high demand and typically transported in such amphorae, it has also been proposed that the amphorae contained wine, with the resin used to seal the interior surfaces and to flavour and/or preserve the wine. To attempt to resolve this question, we have analysed five samples of pistacia resin found in amphorae from the shipwreck using a range of analytical techniques which have used in the past for the analysis of wine residues: spot tests, FT-IR, and HPLC-MS-MS. As well as the archaeological samples, we have analysed modern samples of pistacia resin, leaves and fruit to determine the effectiveness of each technique and to exclude the possibility of false positive results. In addition to the analyses for wine we also detail analysis (GC-MS) of the terpenoids for the purpose of further molecular characterisation of the resin. Bulk stable isotope analysis was used in comparison with similar resins to attempt to identify the geographical origin of the resin.     

Environmental impacts around the time of Norse landnám in the Qorlortoq valley,Eastern Settlement,Greenland

Palynology, radiocarbon dating, and open-section stratigraphies from archaeological trenches are used to examine the impact of human activity around the time of Norse landnám on vegetation and landscape associated with a small farm (Ø34) in the Qorlortoq valley, Eastern Settlement, Greenland (61° N 45° W). Peat deposits from a mire abutting the Norse ruins revealed a discontinuous palaeoenvironmental record containing a possible hiatus from ca. AD 410–1020. Palaeovegetational data were recovered either side of this period. Pollen assemblages suggest that open Salix scrub dominated the landscape during the pre-settlement phase. The later phases of landnám resulted in the creation of hay fields and heavily-grazed grassy heath. Site abandonment is reflected by a re-expansion of Salix. This occurs shortly before the onset of deposition of a Sphagnum peat, dated to cal AD 1420–1630 (2σ) and reflecting an increase in mire surface wetness, probably in response to a deteriorating climate. Radiocarbon dates were obtained on peat and plant macrofossils sampled from either side of the proposed hiatus at two different but closely-spaced (<20 m) locations across the mire. These produced significantly different dates for the cessation of peat formation in the pre-landnám period (cal BC 2130–1770 and cal AD 240–410 respectively), but near-synchronous dates for the recommencement of peat growth (cal AD 890–1150 for peat and a probably more reliable interval of cal AD 1020–1190 based on plant macrofossils). It is suggested that this hiatus may represent the first direct evidence for peat cutting in Norse Greenland.     

Vegetation,land, and wood use at the sites of Bat and Al‐Khashbah in Oman (fourth–third millennium BC)

About 4200 charcoal fragments have been identified from the fourth‐ to third‐millennium BC archaeological sites of Bat and Al‐Khashbah in order to gain an understanding of plant resources available at the sites. Acacia sp., Ziziphus sp., and Tamarix sp. were the main taxa identified at both sites and indicate a similar vegetation composition as today. Phoenix sp. (date palm) charcoal also has been found at both sites. Whereas the cultivation of date palm for the 2700–2300 BC layers from Bat was likely, given other circumstantial evidence (i.e. local cereal cultivation and floodwater irrigation), it is unclear whether date palm was cultivated at Al‐Khashbah. Especially for the older periods (3300–2700 BC) it is possible that nomadic pastoralists were exploiting and/or managing wild date palms. The find of Avicennia marina at Al‐Khashbah indicates long‐distance contacts with the coast.     

Genetic characterization of an archaeological sheep assemblage from South Africa’s Western Cape

The Neolithic Revolution, constituting a shift from food acquisition to food production, came to Africa as it did to most of the rest of the world: through processes of transmission rather than through de novo innovation. In contrast with other regions the pastoral management of cattle, sheep and goats was widespread in Africa thousands of years before settled agricultural communities or the use of domesticated plants were in evidence ( 46, 35 and 10). We report here the discovery of haplogroup B in the first genetic analysis of an African archaeological sheep assemblage.     

Environmental change,agricultural innovation,and the spread of cotton agriculture in the Old World

Recent excavations at the site of Kara-tepe in northwestern Uzbekistan revealed evidence for the production of cotton (Gossypium sp.) in domestic contexts dated to ca. 300–500 AD. These archaeobotanical remains help to document the spread of Old World cotton production, and predate the existing evidence for its cultivation in Central Asia. The context in which these remains were found—in temperate Eurasia at a time of intense environmental and social change—suggests that the spread of cotton agriculture to this region occurred when new plant varieties were incorporated into domestic production regimes as part of local adaptive strategies. The development and transmission of cotton as a global cultigen was initiated by these small-scale innovations aimed at the expansion of economically and environmentally sustainable subsistence practices in Central Asia. Subsequent worldwide transmission occurred when emerging empires helped to spread cotton agriculture more widely across the Old World.     

Ancient olive DNA in pits: preservation,amplification and sequence analysis

The olive tree (Olea europaea) was domesticated by vegetative propagation of selected wild individuals with superior fruit. Later, new cultivars were established repeatedly from feral trees or from crosses between wild, feral, and domesticated trees. Thus the genetic background of many contemporary domesticated lines is a mixture of ancient cultivars and local wild trees. Ancient DNA may illuminate the complicated process of olive domestication because such DNA sequences provide data about ancient genomes that existed closer to the domestication events. Well preserved DNA must be available for such studies, even though in the Mediterranean region, where olive cultivation took place, the climatic conditions are not favorable for DNA preservation. To select for well preserved pits we measured their proportions of lignin by IR spectroscopy, and correlated this with parameters of DNA quality such as template length in an olive-specific repeat array, and template quantity as determined by real-time PCR amplification. Archaeological pits that passed these tests did contain high quality ancient DNA. We present the first ancient olive DNA sequences and compare them to modern wild, feral and domesticated lines.     

The coloured glass of Iulia Felix

Research on the cargo of glass in the Roman ship Iulia Felix, wrecked off the town of Grado (province of Udine, North Italy) in the first half of the 3rd century AD and composed of recycling cullet carefully selected for colour and type, provided much information on Roman glass production technology. A combined approach, involving analytical, statistical, and archaeological evidence, included chemical analyses (X-ray fluorescence, electron microprobe), which indicated that the coloured samples were all soda-lime-silica in composition, with natron as flux, although cluster analysis identified many compositional groups. Comparisons among the compositional groups of the colourless glass, previously studied, and those of coloured glass showed that the production technologies of the colourless glass vessels constitute two well-defined technological end-members, also related to group types, into which those of the coloured glass samples fall. In particular, coloured glass samples—all bottles, low-status vessels—were produced with mainly beach siliceous-feldspar-calcareous sand. However, unlike the colourless type, strict control of raw materials and decolourising processes was not adopted, and recycling was also practised, as demonstrated by the Sb₂O₃, Cu and Pb patterns. In this context, a “recycling index” (RI)—[(Sb₂O₃)_X/(Sb₂O₃)_Ref] * 100—is proposed here for the first time, in order to quantify the extent of recycling of antimony colourless glass in the batch. RI is valid for glass containing abundant Sb from an end-member of colourless glass. In conclusion, although it cannot be stated unequivocally that the identified compositional trends are related to different production centres or different raw materials, the strong evidence of compositional variability among all the Iulia Felix glass samples, both colourless and coloured, supports the dispersed production model for Roman glassware and the common practice of recycling in Roman imperial times, especially for low-status vessels.     

Introduced taro (Colocasia esculenta) and yams (Dioscorea spp.) in Podtanean (2700–1800 years BP) deposits from Mé Auré Cave (WMD007), Moindou,New Caledonia

Plant microfossil analysis was carried out on deposits bearing Podtanean (2700–1800 years BP) ceramics from Mé Auré Cave, Moindou, New Caledonia. Starch residues of introduced Colocasia esculenta, Dioscorea esculenta and one or more, undifferentiated Dioscorea species were identified. The latter include Dioscorea alata, D. bulbifera, D. nummularia and D. pentaphylla. The data provide the first direct evidence of prehistoric horticulture in New Caledonia.     

The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices

Recent archaeological studies of human diet have used stable nitrogen isotope ratios (δ¹⁵N) from human bone collagen to infer the relative importance of terrestrial plant and animal foods. This approach is based on widely observed enrichment of δ¹⁵N up the food chain, plants having distinctly lower values than the herbivores that consume them. Studies of early farming diets in Britain, Denmark and Germany have tended to detect relatively high δ¹⁵N values (e.g. c. +9‰), interpreted as evidence of a diet largely based on animal products, though archaeobotanical evidence for crop cultivation (e.g. carbonised cereal grain and chaff) is widespread. This paper investigates the impact of manuring on δ¹⁵N values in modern cereals, and of charring on these cereal values. The results from two long-term experiments demonstrate that manuring significantly raises δ¹⁵N in cereal grain and chaff. Depending on manuring levels and frequency, it appears that human diets with a major component of such grain would conventionally be interpreted as indicating a largely animal-based diet or a mixed plant/animal diet. Moreover, preliminary analyses of experimentally charred grain and chaff from manured and unmanured conditions are promising for the extraction of reliable ancient δ¹⁵N values from archaeobotanical cereal remains. The wider implications of these results, and the need for further work, are discussed.     

Tuberculosis among Iron Age individuals from Tyva,South Siberia: palaeopathological and biomolecular findings

The study focuses on the evidence for tuberculosis apparent in an Iron Age population recovered from the cemetery of Aymyrlyg, Tyva (Tuva), South Siberia. A recent wholly molecular study of five of the cases confirmed the presence of Mycobacterium tuberculosis (MTB) complex DNA in four of the individuals. In all cases the disease was caused by strains of Mycobacterium bovis rather than Mycobacterium tuberculosis and represents the first positive identification of the bovine form of the disease in archaeological human remains. Details of the palaeopathological characteristics of the cases are provided in the current paper, while the molecular observations are extended to include a quantitative evaluation of the surviving mycobacterial DNA using real-time PCR. The observation that bovine tuberculosis was the pathogen responsible is discussed in terms of current understanding of the evolution of the MTB complex as well as the implications for future ancient DNA studies in this area.     

Middle Stone Age pièces esquillées from Sibudu Cave,South Africa: an initial micro-residue study

In this paper I present the results of a micro-residue study conducted on ten pièces esquillées (scaled pieces) from Sibudu Cave, South Africa. These artefacts are associated with the Howiesons Poort Industry (∼61.7 and ∼64.7 ka years ago at Sibudu), representing part of the later phase of the Middle Stone Age. Until now, it was unclear on what these pieces were used, and whether they were functional. Previous experimental use-wear work tentatively pointed towards bone processing. However, replication work on stone tool production technology suggests that pièces esquillées are merely the by/end-product of bipolar knapping. I used residue analysis on the Sibudu artefacts because this alternative method has the potential to identify if they were used, and if so, illuminate the specific materials the pieces were used on. Although the sample is small, all the pièces esquillées reveal a clear animal processing signal. There are some bone deposits on the utilised edges that may substantiate bone processing, or perhaps a bone hammer was used with them, but additional study, including Later Stone Age artefacts, is needed to assess the feasibility of these observations. It remains possible that the artefacts are core reduced pieces that were subsequently used as tools or simply knapped with a bone hammer.     

Roman agriculture in the conventus Bracaraugustanus (NW Iberia)

A review of archaeobotanical data, together with new, still unpublished data, from Roman sites in conventus Bracaraugustanus suggests the Romanization of the northwest Iberia brought little changes to the agricultural strategies of local communities in indigenous-type settlements. The main crops remain the same as in the Iron Age: Triticum aestivum/durum, Panicum miliaceum, Hordeum vulgare subsp. vulgare, Triticum turgidum subsp. dicoccum and T. aestivum subsp. spelta, Avena and Vicia faba. The first secure reference for the cultivation of Secale cereale in the region comes from a Roman context, suggesting that it was introduced in this period, although it probably remained a minor crop.     

Identifying wine and oil production: analysis of residues from Roman and Late Antique plastered vats

The production of oil and wine was an important aspect of the ancient Mediterranean economy. Among the material remains related to the production of these substances, installations used for their production provide evidence that is still unexploited. Although archaeological studies have been carried out on these installations, problems can arise in the identification of their function, as wine and oil production structures can be similar and the archaeological traces insufficient to establish which substance was produced.