Archaeogenetic study of prehistoric rice remains from Thailand and India: evidence of early <Emphasis Type="Italic">japonica</Emphasis> in South and Southeast Asia

We report a successful extraction and sequencing of ancient DNA from carbonized rice grains (Oryza sativa) from six archaeological sites, including two from India and four from Thailand, ranging in age from ca. 2500 to 1500 BP. In total, 221 archaeological grains were processed by PCR amplification and primary-targeted fragments were sequenced for comparison with modern sequences generated from 112 modern rice populations, including crop and wild varieties. Our results include the genetic sequences from both the chloroplast and the nuclear genomes, based on four markers from the chloroplast and six from the nuclear genome. These markers allow differentiation of indica rice from japonica rice, the two major subspecies of Asian rice (O. sativa) considered to have separate geographical origins. One nuclear marker differentiates tropical and temperate forms of subspecies japonica. Other markers relate to phenotypic variation selected for under domestication, such as non-shattering, grain stickiness (waxy starch) and pericarp colour. Recovery and identification of sequences from nuclear markers was generally poor, whereas recovery of chloroplast sequences was successful, with at least one of four markers recovered in 61 % of archaeological grains. This allowed for successful differentiation of indica or japonica subspecies variety, with japonica identified in all the Thai material and a mixture of indica and japonica chloroplasts in the two Indian assemblages. Rice subspecies was also assessed through conventional archaeobotanical methods relying on grain metrics, based on measurements from 13 modern populations and 499 archaeological grains. Grain metrics also suggest a predominance of japonica-type grains in the Southeast Asian sites and a mixture of japonica and indica in the Indian sites with indica in the minority. The similar results of grain metrics and ancient DNA (aDNA) affirm that grain measurements have some degree of reliability in rice subspecies identification. The study also highlights the great potential of ancient DNA recovery from archaeological rice. The data generated in the present study adds support to the model of rice evolution that includes hybridization between japonica and proto-indica.     

Plant remains and amphorae from the Roman harbour under Flacius Street in Pula (Istria,Croatia)

The aim of this archaeobotanical and archaeological research is to expand knowledge about Roman diet and plant cultivation in Istria and to compare it with similar localities on the Eastern Adriatic coast. We have also tried to find some new information about maritime trade routes in the Mediterranean area in ancient times. Out of 27 samples collected from an excavated Roman port in Flacius Street in Pula, in total, 9809 plant macrofossils were recovered, identified and analysed. The results of the analysis show that most of the plant remains belong to the group of fruit trees and nuts. The most abundant are the remains of Ficus carica, Pinus pinea, Vitis vinifera subsp. vinifera, Rubus fruticosus agg. and Olea europaea subsp. europaea. These are all species that are widespread in the Mediterranean area and have likely always been consumed by the local population. The number of ruderal and weed species found is relatively high (31) in comparison with other plant categories (fruit trees and nuts; fruit collected from the wild; cereals; condiments; oil crops; vegetables and tubers; plants of fresh water environments; elements of maquis), but as they came to the site accidentally and not by targeted human activity, there are far fewer macrofossils of such plants than those of cultivated species. There were a few elements of evergreen forest vegetation and plants of aquatic habitats at the site (2 + 1), which suggests the existence of this type of vegetation in the area of the site in Roman times. Archaeobotanical comparisons of the site in Flacius Street with similar coastal Roman sites—Verige Bay on Veli Brijuni (first-fifth century ad), the port of Zaton near Nin (first-third century ad) and Caska Bay on the island of Pag (first and second century ad)—reveal considerable similarities, confirming the uniformity in nutrition and plant growth in the wider coastal area. Together with the two Roman ships, during the archaeological excavations of the Roman harbour and its layers, we collected over 2000 different archaeological artefacts out of which a large number was almost perfectly preserved. Some of the mentioned artefacts include ceramic amphorae, ceramic table- and kitchenware, ceramic lamps, different usable objects made of glass, wooden use objects, parts of ship’s equipment and other wooden tools, architecture elements from the nearby port as well as residential structures and remains of stone monuments. Because of the large amount of artefacts found at the site, the analysis of the artefacts and data processing are still in progress. As a contribution for recognizing organic remains, we isolated the amphorae whose purpose was the storage and maritime transportation of different food products and ingredients.     

Taphonomic analysis of leporid remains obtained from modern Iberian lynx (Lynx pardinus) scats

Distinguishing leporid bones accumulated by diurnal raptors, owls, mammals and humans is essential to understand not only past human subsistence activities but also past ecology. This is particularly relevant in Iberian Palaeolithic sites where leporid remains usually constitute the most abundant taxon. As far as terrestrial mammal carnivores are concerned, the Iberian lynx (Lynx pardinus) would be the main leporid predator in the Iberian Peninsula. Up until now its contribution as a rabbit bone accumulator in archaeological sites has never been properly assessed. In order to investigate the taphonomic signature of this predator the analysis of 33 samples of modern Iberian lynx scats was carried out. Results show that leporid remains consumed by this carnivore appear very fragmented and exhibit a strong degree of corrosion digestion. Cranial elements are most abundant followed by long bones. Whereas there seem to be clear differences in relation to breakage and digestion alteration caused by owls, some of the patterns obtained are closest to those established with diurnal raptors.     

Skeleton decay of <Emphasis Type="Italic">Cervus elaphus hispanicus</Emphasis> carcasses in Mediterranean ecosystems: biostratinomy at Sierra Norte of Seville natural park (SW Spain)

Taphonomic studies of faunal assemblages from archaeological and palaeontological sites need biostratinomy research to endorse their results. Many papers have studied the relationship between the differential conservation of anatomical parts and the animal responsible for the deposits. In this paper, we present the biostratinomy results from 18 Cervus elaphus hispanicus carcasses (Hilzheimer 1909) in a Mediterranean ecosystem (the Sierra Norte Mountains in Seville Natural Park). We also describe the skeleton decay related to scavenger community, where Sus scrofa (Linnaeus, 1758) is maybe the most important bone scavenger in our study area. These results of this research were compared to those obtained from a similar study carried out in Doñana National Park (Huelva) and confirmed that post-mortem tendencies are similar in both ecosystems.     

Feeding behaviour and taphonomic characterization of non-ingested rabbit remains produced by the Iberian lynx (Lynx pardinus)

The rabbit (Oryctolagus cuniculus) is usually the most abundant taxon found in the Palaeolithic and Epipalaeolithic archaeological sites of southern Europe and the Mediterranean Basin. These accumulations may be the result of different abiotic factors, biotic agents and/or the interaction of both. For these reasons, over the last decades actualistic research has been conducted on different predators of rabbits. Among them, the Iberian lynx (Lynx pardinus) is an excellent candidate for study because: 1) its diet is based almost exclusively on this leporids and 2) its historical distribution overlaps with one of the most interesting areas for small game research, the Iberian Peninsula and southern France. Here we present the taphonomical analysis of non-ingested rabbit remains collected from two Iberian lynx captive breeding centers located in southern Spain. Our results show: i) predominance of the distal segment of the appendicular skeleton; ii) variable breakage patterns, with a high proportion of whole bones of the autopodium and heavy breakage of the zeugopodium and stylopodium; iii) rare bone surface modifications. Comparison of these results with those obtained for other terrestrial carnivores, such as the fox, reveals great similarities, the only difference being the more abundant tooth marks present on fox non-ingested rabbit assemblages.     

Initial Archaeological Survey of the ex-USS <Emphasis Type="Italic">Independence</Emphasis> (CVL-22)

The Boeing Company, collaborating with NOAA to address innovative ways to make ocean observations, provided their autonomous underwater vehicle, Echo Ranger, to conduct the first deep-water archaeological survey of the scuttled aircraft carrier USS Independence in the waters of Monterey Bay National Marine Sanctuary in March 2015. While a preliminary effort, and not comprehensive, the survey confirmed that a sonar feature (previously not proven to be an archaeological feature) charted at the location was Independence, and provided details on the condition of the wreck. At the same time, new information from declassified government reports provided more detail on Independence’s use as a naval test craft for radiological decontamination as well as its use as a repository for radioactive materials at the time of its scuttling in 1951. The wreck is historically significant, but also of archaeological significance as an artifact of the early years of the atomic age and of the Cold War. This article summarizes Independence’s contexts, its nuclear history, and the results of the survey of the wreck site.     

Morphometric distinction between bilobate phytoliths from <Emphasis Type="Italic">Panicum miliaceum</Emphasis> and <Emphasis Type="Italic">Setaria italica</Emphasis> leaves

The development of identification criteria for crop plants based on phytoliths is of high relevance for archaeology, palaeoecology and plant systematics. While identification criteria are available for major food crops, these are mostly based on phytoliths from inflorescences, while other plant parts remain undetected. This paper focuses on bilobate phytoliths from leaves of Panicum miliaceum L. (common millet) and Setaria italica (L.) P. Beauv. (foxtail millet), two taxa that co-occur in regions of Asia and Europe since prehistory and regularly occur at archaeological sites in Eurasia. Leaves of the investigated taxa were systematically sampled to explore the variation of short cells and to collect 27 morphometric variables of bilobate phytoliths with newly developed open-source software. The data was analysed by discriminant analysis, analysis of variance and multiple comparison tests. The resulting morphometric data from five populations per species enables a distinction between the bilobate phytoliths of P. miliaceum and S. italica. Observed differences between populations within species affect only few parameters. This possibility to classify populations of bilobate phytoliths from P. miliaceum and S. italica leaves offers a new method for the detection and identification of these taxa in archaeology, amongst others.     

Scattered shipwreck site prospection: the combined use of numerical modeling and documentary research (<Emphasis Type="Italic">Fougueux</Emphasis>, 1805)

A shipwreck site located off Sancti Petri sand spit (Cadiz, Spain) was identified as a Temeraire-class 74-gun French ship. The archaeological deposits relate it to the Fougueux, sunken after the Battle of Trafalgar. The site contains some pieces of the naval structure, 32 iron guns, and a large anchor, but two thirds of the ship is missing. Available information (including ship logbooks of weather observations and other historic documents) establishes that a dismasted French ship ran aground on 22 October 1805 and broke up into pieces on the 25th due to tempestuous weather. Combining that information and a set of numerical models (ocean, atmosphere, and dispersion), we aim to simulate different scenarios in order to determine the most probable areas for finding the shipwreck remains of the Fougueux, taking into account the uncertainty of the information used and the modeling limitations. The probability of finding scattered remains belonging to the Fougueux was calculated using the modeled scenarios. As a result, it was found that the high probability area contained four additional sites comprising 40 guns and 5 anchors whose characteristics and dating are similar to those of the previously known site. The results of our study highlight the value of numerical modeling in underwater archaeology as a tool for the identification and optimization of resources in prospecting scattered shipwreck sites.     

Identification of woods dating back to the Neolithic Age released from the YENİKAPI (İSTANBUL) excavations

Many ruins dating back to the Neolithic Age have been unearthed at Yenikap? during the archaeological excavations carried out as part of the Marmaray Metro Project. Wood samples of these ruins were sectioned via freezing sliding microtome. These sections became permanent slides and they were compared with reference to slides, wood identification atlases and books in order to identify their taxa. Only 240 samples could be sectioned from among 430 wood samples. As a result of the identification, the following were determined: Quercus spp., Q. pontica, Juniperus spp., Ficus spp., Juglans spp., Fraxinus spp., Alnus glutinosa, Abies spp., Salix sp., Acer spp., Ulmus spp., Castanea sativa and Taxus baccata.     

Who brought in the rabbits? Taphonomical analysis of Mousterian and Solutrean leporid accumulations from Gruta do Caldeirão (Tomar,Portugal)

In the Iberian Peninsula, leporids, and specifically rabbits, play a key role in the understanding of hunter-gatherer economies. They appear to have been especially important in the Tardiglacial, when large numbers of small prey animals and of the European rabbit (Oryctolagus cuniculus) in particular, are a ubiquitous feature of faunal assemblages from archaeological sites. Since a large number of non-human predators can also contribute to the formation of such assemblages, the ability to discriminate between bones accumulated by humans and by other kinds of predators is a key prerequisite to their interpretation. On the basis of systematic actualistic studies carried out on modern leporid remains produced by mammalian terrestrial carnivores, nocturnal and diurnal raptors, and humans, we identified diagnostic taphonomic indicators of the different predators. In this paper, the patterns observed on the modern material are applied to the taphonomical analysis of two archaeological samples of rabbit and hare remains from Mousterian and Solutrean layers of Gruta do Caldeirão, a cave site located in Central Portugal. Our results suggest that Eagle Owl (Bubo bubo) were mainly responsible for the Mousterian accumulations, whilst the Solutrean ones were most likely the result of human activity. These data support the notion that, in Iberia, significant reliance on rabbits does not become a feature of subsistence strategies until later Upper Palaeolithic times.     

The Baelo Claudia Paradigm: The Exploitation of Marine Resources in Roman Cetariae

The Roman city of Baelo Claudia (Baetica, Hispania) is one of the best known halieutic sites of the Mare Nostrum, dating from between the 2nd century BC and the 5th century AD. Recent archaeological investigations have included the discovery, excavation and interdisciplinary study of two new fish-salting factories (so-called “Conjuntos Industriales” XI and XII), providing valuable new information on the exploitation of marine resources. This paper provides the first synthesis of all of the available archaeological evidence for the first three phases of the fishing-product cycle (marine resources/fishing equipment/processing facilities and food products) at this unique site on the Strait of Gibraltar.     

Taphonomy of the bones of rodents consumed by Andean hog-nosed skunks (Conepatus chinga, Carnivora,Mephitidae) in central Argentina

The Andean hog-nosed skunks (Conepatus chinga) are generalized consumers. Rodents are a substantial part of their diet. In order to learn about the effects of this predator on the bones of the prey they consume, the bone remains from scats collected in La Pampa, Argentina, were analyzed from a taphonomic point of view. Analysis confirms that C. chinga produces severe destruction of bone remains, mainly during the chewing process. This allows inclusion of this predator in the category of extreme modifier of the bones of its prey. Data presented here and their interpretation provide criteria to identify the intervention of this predator in archaeological and paleontological sites.     

Refining the <Emphasis Type="Italic">Maritime Foundations of Andean Civilization</Emphasis>: How Plant Fiber Technology Drove Social Complexity During the Preceramic Period

Moseley’s (1975) Maritime Foundations of Andean Civilization hypothesis challenges, in one of humanity’s few pristine hearths of civilization, the axiom that agriculture is necessary for the rise of complex societies. We revisit that hypothesis by setting new findings from La Yerba II (7571–6674 Cal bp) and III (6485–5893 Cal bp), Río Ica estuary, alongside the wider archaeological record for the end of the Middle Preceramic Period on the Peruvian coast. The La Yerba record evinces increasing population, sedentism, and “Broad Spectrum Revolution” features, including early horticulture of Phaseolus and Canavalia beans. Yet unlike further north, these changes failed to presage the florescence of monumental civilization during the subsequent Late Preceramic Period. Instead, the south coast saw a profound “archaeological silence.” These contrasting trajectories had little to do with any relative differences in marine resources, but rather to restrictions on the terrestrial resources that determined a society’s capacity to intensify exploitation of those marine resources. We explain this apparent miscarriage of the Maritime Foundations of Andean Civilization (MFAC) hypothesis on the south coast of Peru by proposing more explicit links than hitherto, between the detailed technological aspects of marine exploitation using plant fibers to make fishing nets and the emergence of social complexity on the coast of Peru. Rather than because of any significant advantages in quality, it was the potential for increased quantities of production, inherent in the shift from gathered wild Asclepias bast fibers to cultivated cotton, that inadvertently precipitated revolutionary social change. Thereby refined, the MFAC hypothesis duly emerges more persuasive than ever.     

Why does (archaeological) micromorphology have such little traction in (geo)archaeology?

Archaeological deposits are often complex and illustrative of an intricate interplay between geogenic and anthropogenic inputs and formation processes. Even for those archaeologists—particularly prehistorians—who consider the basic principles of natural stratigraphy to excavate their sites, they nonetheless typically underutilize the observations and data available at the microstratigraphic level. The technique of soil micromorphology—or archaeological micromorphology as referred to throughout this paper—has seen an astounding increase in its use to answer archaeological questions and archaeological sediments in the last decades. However, we consider that this tool is still quite underutilized and not as mainstream as other techniques. In this paper, we briefly reflect on what can be some of the causes underlying this situation and how we (that is, both producers and consumers of micromorphology data) can go about to change it. The main idea is that we need to establish a better and more approachable way to present micromorphological results and be better at integrating them with the macroscopic archaeological data and research questions.     

Satellite Remote Sensing of Archaeological Vegetation Signatures in Coastal West Africa

This article discusses how archaeological sites in Sierra Leone, and by extension much of West Africa, can be identified through vegetation patterns (vegetation signatures) detectable in very high-resolution (VHR) multispectral satellite imagery. Settlement sites typically have a differing pattern of vegetation from the surrounding landscape, including concentrations of very large trees with sociocultural and historical significance: cotton (Ceiba pentandra) and baobab (Adansonia digitata). These features are conspicuous elements of the landscape both from the ground and in aerial imagery. Two complementary methods of using VHR multispectral satellite imagery are discussed in this paper: visual interpretation and semi-automated subpixel classification. These techniques are aiding ongoing archaeological survey of the Sierra Leone River Estuary. The impact of recently renewed industrial activity at a site of probable archaeological significance is also assessed through visually interpreted VHR satellite imagery.     

Quotidian and Transgressive Practices in Nazi Forced Labor Camps: The Role of Objects

We argue that archaeological investigations of Nazi-period Lager should include two elements. The first is excavation. Research confined to non-invasive methods documenting visible remains, archival research, and oral history leads to small gains in existing knowledge. Archaeology’s full potential is realized when excavations are conducted allowing reconstruction of actual practices. Secondly, it is essential to evaluate spatially and quantitatively even the most inconspicuous objects. They are a fundamental means to understand conditions of suffering as well as transgressive actions by camp inhabitants. Excavations of a forced labor camp of Weser Flugzeugbau GmbH at Tempelhof airfield in Berlin serves as a case study.     

Elucidating post-harvest practices involved in the processing of algarrobo (<Emphasis Type="Italic">Prosopis</Emphasis> spp.) for food at El Shincal Inka site (Northwest Argentina): an experimental approach based on charred remains

It is known from previous studies that qualitative and quantitative morphological attributes of food products and residues may permit the identification of different food processing activities in desiccated algarrobo (Prosopis chilensis and Prosopis flexuosa) remains. Experimental approaches are used here in order to (1) evaluate if those diagnostic features persist even after charring and (2) to use these results as a means of interpreting types of processing from archaeobotanical contexts of the Inka site of El Shincal. Experimentation was made on the basis of traditional practices which were registered during previous ethnobotanical work in the area. Diagnostic patterns of Prosopis flours and patay (bread), as well as of añapa/aloja (fresh and alcoholic beverages, respectively) and arrope (syrup) residues were stipulated for charred remains. In the specific case of El Shincal, specimens derived from flour manufacture (unrefined and refined) and añapa/aloja residues were recognized from different buildings. This must have implied a special organization of the production and the people working on that, in the sense of post-harvest intensification.     

USS <Emphasis Type="Italic">Independence’s</Emphasis> Aircraft

Submerged aircraft are a newly emerging area of study in underwater archaeology. In August 2016, a multidisciplinary team conducted the first archaeological survey of the deep water (829 m) wreck of the ex-USS Independence (CVL22), a WWII era light carrier scuttled off San Francisco after its use as an atomic bomb test target and subsequently as a floating radiological laboratory and training facility. Using the remotely operated vehicles Argus and Hercules, the expedition documented and studied Independence including assessment of a sonar target thought to be an aircraft resting inside the sunken carrier’s forward elevator pit. The survey confirmed the presence of a plane, but previous assumptions as to the type and identity of this aircraft, based on archival records, proved erroneous. As well, the remains of a second, and possibly a third, aircraft were also encountered during the 2016 survey. These artifacts, through their context, both as naval warplanes on an aircraft carrier, and as test articles for seaborne nuclear weapons development, more precisely fit within the parameters of maritime archaeology. They join other archaeological evidence at the Independence site that helps define its significance within the context of World War II and the Cold War.     

Experimental studies of personal ornaments from the Iron Gates Mesolithic

Personal ornaments, especially those made from the shells of marine mollusks and animal teeth, have been recovered from many Mesolithic sites across Europe. This paper reviews the evidence of personal ornaments from the Mesolithic of the Iron Gates, where such finds were identified in five sites on the Romanian bank: the cave and rock shelter sites of Climente II and Cuina Turcului, and three open-air sites—Icoana, Ostrovul Banului, and Schela Cladovei. The ornaments from these sites were made from the shells of several gastropod taxa and at least one species of dentaliid scaphopod, as well as the pharyngeal teeth of cyprinids, the teeth of several species of terrestrial mammal, fish vertebrae, and pieces of antler and bone. Particular attention is given to taxonomic identification and questions of taphonomy, provenance, selection, manufacture, and use. Experiments were conducted in which several types of ornament were replicated. Archeological and experimental pieces (at various stages of production) were examined under a microscope, to establish the durability of the beads and estimate the length of time over which they were worn. Our results show that shells of Lithoglyphus and Theodoxus sp. were simply perforated and fixed in composed adornments, as were shells of Tritia neritea in the early part of the time range. In the later Mesolithic, T. neritea shells were processed in a different way and fixed to clothing in the manner of appliqués. No technological modification of the cyprinid teeth was observed; these were sewn individually onto clothing by means of a thread coated with an adhesive substance. The other categories of personal ornament were used mainly as pendants. Experimental use-wear analysis suggests that many ornaments were used over long periods, with broken or missing pieces replaced when necessary.