New Insights into Mesolithic Human Diet in the Mediterranean from Stable Isotope Analysis: The Sites of Campu Stefanu and Torre d'Aquila,Corsica

Mesolithic human remains are rare in the archaeological record of the French Mediterranean. Only the island of Corsica has so far produced relatively well‐preserved burials, and recent archaeological excavations have brought to light new Mesolithic human remains. The site of Campu Stefanu , located in Sollacaro in the southeast of the island, contained a collective burial of seven to eight individuals in a previously unobserved funerary context. A re‐evaluation of collections in regional museums yielded the remains from another Mesolithic individual from the site of Torre d 'Aquila , excavated at Pietracorbara, in the northern part of the island, at the beginning of the 1990s. These two discoveries presented the rare opportunity to obtain new radiocarbon dates and paleodietary insights from this crucial time period using stable isotope analysis (δ¹³C, δ¹⁵N) on collagen. From Campu Stefanu, one individual had sufficient collagen preserved for radiocarbon dating, revealing that it is the oldest Mesolithic human known on the island, dated to 10216–9920 cal. BP. At Torre d'Aquila, radiocarbon dates indicate that the individual belonged to a younger Mesolithic phase than Campu Stefanu, dated to 9903–9596 cal. BP. δ¹³C and δ¹⁵N isotope ratios are similar between the Campu Stefanu and Torre d'Aquila individuals and indicate a diet dominated by the consumption of terrestrial animal protein and a lack of marine resources. These findings are in contrast with the previous results from two other Mesolithic individuals from Corsica from the sites of Araguina Sennola and Monte Leone , for which about 25–30% of the consumed proteins came from a marine diet. The dietary variability recorded in Corsica is consistent with results obtained from Mesolithic human remains of Sicily and the Iberian Peninsula. We can hypothesise, that despite the nomadic lifestyle, the distance to the sea played a major role in Mesolithic food choices in Corsica. Copyright © 2016 John Wiley & Sons, Ltd.     

The Potential of EBSD and EDS for Ceramics Investigations—Case Studies on Sherds of Pre‐Columbian Pottery

The work focuses on the potential of structural and chemical examinations by scanning electron microscopy based methods for archaeometric studies on ceramics. Achieved by a single preparation technique (polished block sections), the feasibility and benefits of electron backscatter diffraction are demonstrated as case studies using polychrome examples of pre‐Columbian pottery (Wari, Moche and Cajamarca). Elemental and phase maps allow for separate consideration of clay and temper. Identification of mineral phases and intergrowths of temper particles provide information for clarifying clay procurement and firing techniques with respect to local versus non‐local pottery to enlighten trade relations, technological transfer and shared heritage of pre‐Columbian cultures.     

Assessing the life history of an andean traveller through biogeochemistry: Stable and radiogenic isotope analyses of archaeological human remains from Northern Chile

Bioarchaeology and biogeochemistry can elucidate aspects of individual life histories that are often lost in the archaeological record. Here, we use stable and radiogenic isotope analyses of enamel, bone and hair to reconstruct paleodiet and paleomobility in an adult male interred along a pre‐Columbian route connecting the northern Chilean coast to the inland Loa River Valley. Although this well‐preserved burial included mortuary goods typical of coastal cultures, it was discovered in a vast, uninhabited part of northern Chile's hyper‐arid Atacama Desert. Variation in carbon and nitrogen isotopes reflects dietary differences, while strontium and oxygen isotopes vary geologically and geographically. We use these data to examine paleodiet and paleomobility and to assess whether this was a coastal traveller seeking provisions from the interior or vice versa. Enamel stable isotope analysis is consistent with the consumption of a mixture of terrestrial and marine resources during the first years of life. Bone stable isotope analyses indicate habitual consumption of marine foodstuffs over the last 10–30 years of this individual's life. Interestingly, stable isotope analysis of hair samples provides more fine‐grained information on this individual, suggesting movements between the coast and highlands in the months before his death. Radiogenic strontium isotope data are consistent with residence on the coast or in the Atacama Desert, but are lower than strontium isotope values from higher altitudes. These dietary and geological patterns are reconcilable with coastal residency; the isotopic data are consistent with foodstuffs and textiles found with the burial. Therefore, we argue that this individual was regularly moving from the coast to inland areas, crossing the hyper‐arid Atacama Desert by following strategic interzonal routes that provided access to particular resources. Copyright © 2010 John Wiley & Sons, Ltd.     

Palaeobiology of the Medieval Population of Albano (Rome,Italy): A Combined Morphological and Biomolecular Approach

Recent years have seen increased interest in skeletal populations from the Imperial Roman Age in Italy, but much less is known about diet and standards of living in the subsequent medieval period. To fill this gap, we conducted a morphological analysis of human remains from Albano, an Italian town near Rome, as well as a stable isotope analysis of bone collagen to reconstruct diet. The sample was recovered from a Medieval cemetery (1040–1220 cal. yr. BP) located in the gardens of the historical Palazzo Doria Pamphili in Albano. A minimum number of 40 individuals (31 adults and 9 sub‐adults) were examined using standard methods. Though the general health status of the population was good, signs of cribra orbitalia and diffuse enthesopathies were noted during the morphological examination. Stable carbon and nitrogen isotope analyses of the bone collagen from 24 adult humans and three faunal bones indicate that the diet of the population may be described as predominantly terrestrial and C₃‐plant based although the data for some of the individuals suggest a modest consumption of C₄‐(millet) based or aquatic proteins. No evidence of significant dietary differences between the sexes was found. The comparison of the isotope data from Albano with those from populations recovered in the same region is consistent with a shift from a terrestrial, possibly plant foods‐dominated subsistence in the Early Middle Ages to a diet with a higher contribution from animal proteins, both terrestrial and aquatic, in the Later Middle Ages. Copyright © 2013 John Wiley & Sons, Ltd.     

Diet,nutritional status and oral health in hunter‐gatherers from the central‐northern coast of Patagonia and the Chubut river lower valley,Argentina

Archaeological research in central‐northern Patagonia (Atlantic coast and lower the valley of Chubut river) showed that this area was used since at least the Middle Holocene. Stable isotope analyses (¹³C and ¹⁵N) of human bone samples indicate that hunter‐gatherers living in that area had a terrestrial‐marine diet including guanaco meat, land plants, mollusks and pinnipeds. Despite this general trend, intersite variability and changes through time were noted, especially after the late Holocene. These results have been reinforced by archaeofaunal, technological and bioarchaeological records. In this paper, three hypotheses are examined: (a) the diet of these populations was complete and rich enough to ensure good health status and avoid nutritional deficiencies; (b) carbohydrate consumption increased progressively after 1000 BP, when pottery technology was adopted and (c) this kind of mixed diet would have been qualitatively more nutritious than that of other populations of the region, which would have resulted in better nutritional and healthy conditions. These three hypotheses are compared with dental results obtained from 563 permanent teeth from 45 individuals (34 adults and 11 juveniles from both sexes), rescued from burial sites. Indicators of oral health were assessed through the observation of caries, abscesses, wear, pulpar cavity exposure and ante mortem loss. Features of nutritional status such as enamel hypoplasia, porotic hyperostosis and cribra orbitalia were also examined. Given the availability of direct radiocarbon dating for most of the sample, three temporal series were determined: ‘Before 1000 BP’, ‘1000–5000 BP’ and ‘Post‐contact’. No evidence of alimentary stress or iron deficiency was found in individuals from the three series, which accounts for healthy and good nutritional life conditions. After 1000 BP, the results show a progressive increase in the caries percentage and a decrease in abscesses, dental wear and ante mortem losses frequency. This is possibly related to more consumption of processed foods in the last 1000 years. These results were compared with similar studies based on samples from different environments and latitudes of Patagonia. Evidence suggests that mixed diets (marine‐terrestrial) would have been more appropriate and nutritionally complete than exclusively marine or terrestrial diets. Copyright © 2010 John Wiley & Sons, Ltd.     

MOLECULAR AND ISOTOPIC DEMONSTRATION OF THE PROCESSING OF AQUATIC PRODUCTS IN NORTHERN EUROPEAN PREHISTORIC POTTERY*

A number of charred surface residues, adhering to ceramic containers, were obtained from various coastal and inland sites in north‐west Europe dating from the sixth to the fourth millennium cal bc . In order to investigate the use of these vessels and in particular to identify any marine products, the residues were subjected to carbon and nitrogen stable isotope analysis. Variation in carbon isotope ratios between different samples indicated that some vessels may have been used to process marine products. This analysis was corroborated by specific identification of aquatic products following structural and isotopic characterization of lipids extracted from selected samples.     

Seasonal Patterns of Coastal Exploitation on the Farasan Islands,Saudi Arabia

Here we present the results of the analysis of coastal exploitation patterns in the southern Red Sea during the Middle Holocene. We focus on the shell midden cluster of the Farasan Islands, Saudi Arabia, which comprises over 3,000 shell midden sites. These sites date from 6,500 to 4,500 cal BP and are part of an arid landscape. We focus on one site, JW1727, which provides a snapshot of marine exploitation and will help to understand the use of food resources within the region. Stable isotope values (δ¹⁸O) were collected from the marine gastropod Conomurex fasciatus (Born 1778), which represents 72% of shell weight of JW1727, in order to reconstruct the season of capture. Results demonstrate that 1) every season is represented within the dataset; and 2) there is increased C. fasciatus deposition during the summer and autumn months. This indicates a diet consisting of C. fasciatus throughout the year in combination with other food sources and an increase of the C. fasciatus component during the arid seasons, possibly linked to the unavailability of vegetation. Additionally, size measurements of C. fasciatus were carried out to examine changes in size distribution throughout the occupation of the site that could be related to overexploitation of C. fasciatus. However, no significant trends could be observed. In sum, the results suggest a sustainable and constant habitation of the Farasan Islands despite the highly arid conditions.     

Paleodietary analysis of a San Francisco Bay Area shellmound: stable carbon and nitrogen isotope analysis of late Holocene humans from the Ellis Landing site (CA-CCO-295)

The late Holocene archaeofaunal record of the San Francisco Bay Area demonstrates temporal declines in the abundance of low-cost, high-ranked marine and terrestrial resources. During later periods of occupation, faunal assemblages are often dominated by lower-ranked, higher-cost resources, suggesting an increase in diet breadth through time. Archaeological resource intensification models argue that this marks a late Holocene decline in foraging efficiency in the Bay Area, driven by human-induced harvest pressure. This study examines dietary change in the region using stable carbon and nitrogen isotope data from 65 human burials, spanning two temporal components (cal AD 55–890 and cal AD 762–1550) at the Ellis Landing site (CA-CCO-295). The strong linear relationship between collagen carbon and nitrogen isotope values reflects both marine and terrestrial food consumption, with individuals showing a high level of dietary variability at the site. No temporal trend or meaningful sex differences were found in isotope values. The widening of diet breadth predicted by the archaeofaunal record occurred prior to the occupation of the Ellis Landing shellmound site. The trend in exploiting a greater amount of terrestrial resources during the Middle and Late Period in central California was supported by isotopic evidence.     

Incisor microwear textures of five bioarcheological groups

Dental microwear texture analysis has proven to be a valuable tool for inferring aspects of subsistence behaviour in human groups and diet in other mammals. Studies have to date been limited to molar teeth. Here we report on the first microwear texture analysis of incisors. Five bioarcheological groups were included in this analysis: Aleuts from various islands in the Bering Sea (n = 24), Arikara from the Mobridge site in South Dakota (n = 18), ethnic Chinese cannery workers from Kodiak Island (n = 16), a Late Woodland Bluff sample from Jersey County, Illinois (n = 18) and Puye Pueblo from Pajarito Plateau in New Mexico (n = 18). First, point clouds with 0.18 µm lateral spacing and 0.005 µm vertical resolution were obtained from maxillary central incisor labial surfaces using a white‐light confocal profiler. Four adjoining fields were sampled for a total area of 276 µm × 204 µm for each specimen. Surface data were then imported into scale‐sensitive fractal analysis software for texture characterisation. Results indicate significant variation among groups in anisotropy, fill volume and heterogeneity. These differences are likely related to differences in diet, degrees and types of non‐dietary incisor use, as well as exposure to abrasives. This study also suggests that texture variables most useful for characterising and comparing incisor microwear may differ from those most useful for distinguishing molar microwear patterns. Copyright © 2009 John Wiley & Sons, Ltd.     

CONSTRUCTING CARIBBEAN CHRONOLOGIES: COMPARATIVE RADIOCARBON DATING OF SHELL AND WOOD ARTEFACTS FROM PRE‐COLUMBIAN SITES IN CUBA

The lack of robust chronological frameworks for pre‐Columbian archaeology in the Caribbean limits the interpretive potential of ongoing studies of inter‐site and inter‐island interaction. This paper reviews the dating of 28 wood and shell artefacts from sites in northern Cuba in an attempt to date pre‐Columbian activity in the region and develop dating methodologies for the study of pre‐Columbian interaction in the Caribbean. This research compares dates from wood and shell artefacts from the waterlogged site of Los Buchillones in north‐central Cuba with dates from shell artefacts from sites on eight islands in the Sabana–Camaguey archipelago in the Bahama Channel. In addition to providing a chronological framework based on absolute dates for pre‐Columbian activity in northern Cuba, this paper also considers the methodological issues of using shell for dating pre‐Columbian activity in the Caribbean.     

Treponemal Disease,Tuberculosis and Subsistence‐settlement Pattern in the Late Woodland Period West‐central Illinois

In later pre‐Columbian prehistory (post AD 1000), the adaptation and intensification of maize agriculture and its correlate of aggregate village settlement (i.e. Mississippianization) is temporally and geographically variable. In the Midwest, consequential to the florescence of the major ceremonial centre of Cahokia (AD 1050–1300), the Mississippi River Valley alluvial plain in Illinois, known as the American Bottom, became a core area of this subsistence‐settlement change. Much archaeological research has traced aspects of this transition in the Lower and Middle Illinois River Valley, but little is known outside of these areas. A skeletal sample from the remote hinterland area of the Upper Mississippi River in west‐central Illinois was examined for arguable paleopathological correlates of sedentism (treponemal disease) and Mississippianization (tuberculosis). The Schroeder Mounds (AD 900–1100) adult skeletal sample (N = 53) exhibited a high frequency of treponemal disease (13.2–15.1%). This result is consistent with paleopathological literature linking a 9+ % pre‐Columbian North American prevalence with sedentism, challenging archaeologically based inferences that the hinterland was occupied by mobile forager‐horticulturalists. A hallmark of Mississippianization is the presence of diagnostic cases of tuberculosis. No cases were observed in the Schroeder sample, suggesting a pre‐Mississippian subsistence‐settlement pattern. Copyright © 2013 John Wiley & Sons, Ltd.     

DISCOVERY AND CHARACTERIZATION OF AN UNKNOWN BLUE‐GREEN MAYA PIGMENT: VESZELYITE*

Blue‐green mosaic and polychrome masks and funerary offerings from the royal tombs of Calakmul, Mexico, were analysed by scanning electron microscope equipped with energy dispersive X‐ray spectroscopy (SEM‐EDS), X‐ray diffraction (XRD), and particle induced X‐ray emission (PIXE). This led to the first identification of the use of veszelyite, a rare hydrated copper‐zinc phosphate, as green pigment. Analyses of a geological sample of this mineral have been done to confirm the characterization of this Mayan pigment, which might help determine pre‐Columbian trade routes of precious and luxury objects in the ancient Maya Classic period (ad 250–800).     

FINGERPRINTING OF ROMAN MINTS USING LASER‐ABLATION MC–ICP–MS LEAD ISOTOPE ANALYSIS*

In this paper we present data to demonstrate the applicability of laser‐ablation MC–ICP–MS isotope analysis to archaeological artefacts, in this case Roman silver coins. The technique requires no chemical preparation, does minimal damage to the sample and yields external reproducibility that is better than conventional TIMS analysis; ²⁰⁷Pb/²⁰⁶Pb =±0.015% 2σ in comparison with ²⁰⁷Pb/²⁰⁶Pb =±0.04% 2σ, respectively. We show that Pb isotope compositions give isotope fingerprints to mints despite the likely reworking of the metal during coin production.     

Stable isotope evidence for dietary and cultural change over the Holocene at the Sabana de Bogotá region,Northern South America

The Sabana de Bogotá in the northern Andes is an interesting region to investigate temporal patterns of dietary variation because it counts with a vast archeological and osteological record for the last 10,000 years. In this paper, stable isotope data of human archeological bone collagen and apatite were used to study the evolution of diet and major subsistence transformations taking place during the Holocene (～9000–600 cal BP). Paleoenvironmental reconstructions and the isotopic ecology of the Sabana de Bogotá were used as an interpretative baseline. Stable isotope measurements (δ¹³C_col, δ¹³C_ap, δ¹⁵N, and Δ¹³C_col-ap) representing hunter-gatherers, horticulturalists, and agriculturalists (N = 134 individuals) were analyzed by using bivariate, regressional, and discriminant statistical techniques. Results show that early Holocene hunter-gatherers (9000–7000 cal BP) consumed mostly C₃ vegetal resources locally available. In contrast, animal protein was less important. Middle Holocene hunter-gatherers (6000–4500 cal BP) continued with the food foraging pattern observed in the earlier counterparts and presented a slight increase in C₃ animal protein intake. During the initial late Holocene ca. 4000 cal BP, important shifts in subsistence strategies occurred when populations presented a trend toward mixed C₃/C₄ diets, and by ca. 3500 cal BP, there is a clear signal of C₄ crops (i.e., maize) consumption concomitant with the introduction of ceramic technology. During the final late Holocene (last 2000 cal years BP), intensive agriculture was adopted and humans presented relatively diverse diets integrated by C₄ and C₃ crops, C₃-C₄ feeding animals, and freshwater resources. Such dietary change coincides with an increase in sociopolitical complexity, population size, and a general decline in health.     

Carbon and nitrogen stable isotope analysis on the diet of Jomon populations from two coastal regions of Japan

We report on a stable isotope paleodietary reconstruction of Jomon populations in Japan during the Middle to Final Jomon period (ca. 5000–2300 years BP), focusing on dietary differences within and among populations and between regions. Carbon and nitrogen isotope analysis was performed on human and faunal bone collagen from six coastal sites along the Inland Sea in the Sanyo (Ota, Funamoto, and Tsukumo) region and along Mikawa Bay and the Pacific Ocean in the Tokai (Kawaji, Yoshigo, and Inariyama) region. We found that carbon and nitrogen isotope ratios were positively correlated, indicating that the Jomon people consumed a mixed diet of marine (shellfish and marine fish) and terrestrial (C₃ plants and terrestrial mammals) protein. In the Ota samples (n = 25, during the Middle Jomon period, 5000–4000 years BP), sex was one of the main reasons for the intra-population dietary variation. Ota males consumed greater amounts of marine food, while Ota females consumed greater amounts of terrestrial food; these dissimilar diets may have been related to the sexual division of labor. Significant inter-population dietary differences were found, which may have been related to differences in age or site location. Notably, the two coastal regions showed clear isotopic differences. Nitrogen isotope ratios of individuals from the Sanyo region were significantly higher than ratios of individuals from the Tokai region. The individuals in the Sanyo region might have consumed a diet high in aquatic foods, particularly high trophic level marine fish, whereas the individuals in the Tokai region might have consumed a lot of marine shellfish. Another possible reason for the regional isotopic difference might have been different baseline of nitrogen isotope ratios of the marine ecosystems.     

Dietary change in Fijian prehistory: isotopic analyses of human and animal skeletal material

We examine evidence for prehistoric diet in the Fiji Islands through the analysis of stable isotope ratios (δ¹³C and δ¹⁵N) for 26 samples of human and animal bones from various archaeological sites, time periods, and local environments. The oldest individuals in this study, dating to about 2700 BP and living on the small island of Waya, consumed a predominantly marine-based diet. Subsequent populations on this island showed reduced consumption of marine resources, with greater reliance on terrestrial ones, throughout the cultural sequence. In contrast, populations of humans and pigs living inland on Viti Levu, the largest island, relied on terrestrial resources since at least 1500 BP. Thus, our results suggest that human and pig diets throughout Fijian prehistory relied variably on marine or terrestrial resources, and this distinction is largely a product of geography. This finding and our analyses provide a model for understanding ancient diets in Remote Oceania.     

THE AMBER OF EL DORADO: CLASS IB ARCHAEOLOGICAL AMBERS ASSOCIATED WITH LAGUNA GUATAVITA*

Laguna Guatavita, a crater‐like lake located in central Colombia, was used by pre‐Columbian Muisca people for a variety of socio‐religious ceremonies, many of which involved casting offerings into the lake. Of these, the installation of a new ‘king’ became the basis of the El Dorado myth that was carried back to Europe by 16th century conquistadores. In the present study, beads collected from Laguna Guatavita during a late 19th/early 20th century attempt to recover valuables from the lake bed, have been subjected to geochemical analysis. Results establish that these artefacts have been fabricated from Class Ib amber. Class Ib ambers have not previously been reported in either the geological or archaeological records of Central or South America, and their existence in this context implies that the Muisca people had access to a unique local or regional source of amber, knowledge of which has subsequently been lost.     

Strontium isotope evidence of migration and diet in relation to ritual tooth ablation: a case study from the Inariyama Jomon site,Japan

Ritual tooth ablation was extensively practiced among Jomon (Japanese Neolithic) societies in their final phase (ca. 3000-2300 BP). This tradition includes two different tooth ablation patterns, type 4I and type 2C, referring to extraction of the mandibular incisors and canines, respectively. However, the reason for this difference is unclear. Previous carbon and nitrogen stable isotope analysis of human remains from the Inariyama shell mound revealed that type 4I individuals were more dependent on terrestrial resources and type 2C individuals on marine resources. To test this hypothesis, we performed strontium (Sr) isotope (⁸⁷Sr/⁸⁶Sr) analyses on the same skeletal remains and on modern plants around the site. Because Sr isotope ratios of plants differ according to the local geology and seawater has a consistent Sr isotope ratio, the Sr isotope ratios of tooth enamel can reveal both migration and diet. Comparing Sr isotope ratios in plants and seawater with those of tooth enamel, we identified four possible immigrants. Type 4I locals had significantly higher Sr isotope ratios than type 2C locals. The ratios of the type 4I and type 2C locals were close to those of terrestrial plants and seawater, respectively, suggesting that type 4I locals had incorporated much Sr from terrestrial resources and type 2C locals from marine resources. These results support the hypothesis that ritual tooth ablation reflects dietary differences throughout an individual’s life, and they suggest possible occupational differentiation among the Jomon people.     

Infancy and childhood in the pre‐Columbian North American Southwest–first results of the palaeopathological investigation of the skeletons from the Grasshopper Pueblo,Arizona

Infant and child skeletons (n = 369) from the pre‐Columbian Grasshopper Pueblo site in east‐central Arizona (US) dating from the 14^th century AD were examined macroscopically and with low‐power microscopy. They were studied as a representative example of a typical Mogollon community with respect to frequencies of deficiency and inflammatory diseases. First results revealed very poor living conditions, which are characteristic for this time period in the North American Southwest because of lack of food due to climatic and political changes. Thus, non‐specific stress indicators were frequently observed. In the group of deficiency diseases, anaemia was found in more than 50% of individuals, and in the group of inflammatory diseases, meningeal irritations were diagnosed in more than 70%. Copyright © 2007 John Wiley & Sons, Ltd.     

Comments on economic change and the prehistoric fur trade in Northern Sweden

In this study, we used stable isotope analysis and radiocarbon dating to study diet, mobility and chronology in two late medieval/historical coastal populations in northern Norway. We have shown that the individuals buried at Kirkegårdsøya date between 1331 and 1953 cal AD and had a homogenous marine diet, whereas the individuals buried at Gullholmen had a more heterogeneous diet, consisting of both terrestrial and marine proteins and date between 1661 and 1953 cal AD. We have demonstrated that reindeer protein was not an important part of their diet, and also discussed the importance of correcting for the marine reservoir effect in populations with a coastal subsistence. Our interpretation is that individuals buried at Kirkegårdsøya primarily belonged to a Coastal Sámi community, although Norwegians with a similar diet (and likely comprising a minor population in the area) cannot be ruled out. The more varied diet and mobility at Gullholmen could, as predicted, indicate that these individuals may have had a more diverse cultural affinity.