Problems and pitfalls in the diagnosis of linear enamel hypoplasia in cheek teeth of cattle

Prevalence and intensity of enamel hypoplasia have been used as markers of generalized physiological stress during dental development in a wide range of mammalian taxa. We studied cattle (Bos taurus) cheek teeth exhibiting morphological characteristics that are of relevance to the diagnosis of enamel hypoplasia in this and other bovid species. These characteristics were multiple, more or less horizontally arranged (waveform) lines or grooves in the cementum of the tooth crown and the adjacent root area, leading to an imbricated appearance of the cementum. On macroscopic examination of tooth surfaces, these lines resembled linear enamel hypoplasia (LEH). Microscopic analysis of tooth sections, however, revealed that the lines occurred in the cementum only, and that the underlying enamel did not exhibit morphological irregularities. In cheek teeth of older cattle, a thick cementum layer is regularly found in the cervical crown portion and the adjacent root area. Apposition of this cementum is related to the uplifting of the teeth from their alveoli, a process that compensates for the shortening of the tooth crowns due to occlusal wear. In the studied specimens, a pronounced periodic nature of tooth uplifting and the related deposition of cementum is the likely cause for the observed imbricated appearance of the cementum. While this phenomenon may be misinterpreted as representing a case of LEH, presence of enamel hypoplasia in bovid teeth may be overlooked when the defects become filled with coronal cementum and are therefore not apparent on external inspection. This was the case in one of the cattle teeth analyzed by us, in which the hypoplastic enamel defects were, however, clearly discernible in ground sections. Microscopic analysis of tooth sections is recommended for recording of LEH in bovid teeth in cases where macroscopic examination of tooth surfaces alone does not produce unequivocal results.     

The Relationship Between Linear Enamel Hypoplasia and Social Status in 18th to 19th Century Edo,Japan

Linear enamel hypoplasia (LEH) has been utilised in human bioarchaeology as an indicator of growth disturbance during childhood. However, only a few studies have compared populations of different socio‐economic status in the same time period. This study examines the association between the LEH occurrence pattern and social status in the 18th to 19th century populations in Japan. Detailed archaeological studies on burials from the Edo period (1603–1867) excavated in Tokyo have facilitated estimating an individual's social status by its burial type. In this study, 112 individuals from the Sugenji‐Shokenji site were divided into three burial structure groups (ceramic jar coffins for middle‐class warriors, wooden square coffins for low‐class warriors and townspeople and wooden circular coffins for townspeople) and examined for the general prevalence of LEH, number of LEH and the chronological distribution of LEH. A high general prevalence of LEH was observed in every group, especially in lower canine (79.3–100.0%). However, individuals in the jar coffin group showed a lower prevalence and smaller number of LEH per tooth, suggesting that individuals of higher social status experienced better living environments in their childhood. There was no significant difference in the chronological distribution of LEH formation between coffin groups. Such data are essential for understanding the association between socio‐economic status and living conditions in specific societies in the past. Copyright © 2015 John Wiley & Sons, Ltd.     

The necropolis of Vallerano (Rome, 2nd–3rd century AD): an anthropological perspective on the ancient Romans in the Suburbium

The present study investigates the skeletal remains of individuals who were part of a Roman suburban community, in order to assess lifestyle and living conditions in the town's outskirts during the Roman Imperial age. The existence of the community was linked to the functioning of one of the many villas that surrounded the town of Rome at that time. In order to assess health, several indicators were explored, including mortality, oral pathologies and specific (cribra orbitalia) and aspecific (linear enamel hypoplasia) indicators of nutritional and physiological impairment. The sample, which probably represents the labour force of the villa, shows a high number of individuals dying in the early adult age and very few living beyond 50. Subadults were frequently affected by pathological conditions which may indicate anaemia and/or inflammations and infections, as witnessed by the frequency of cribra orbitalia. Growth was also impaired, as the individuals suffered from systemic disturbances during the early years of life that led to the formation of linear enamel hypoplasia (LEH) in their teeth. Frequency of LEH is very high, as well as its multiple occurrence through time (2.44 defects per individual) and its onset occurs from the earliest age classes. Diet, on the other hand, does not seem to have been particularly carbohydrate based. Oral pathologies are very low, which is consistent with meat consumption complementing a diet rich in low‐calorific products of agriculture and seemingly low in refined carbohydrates. Copyright © 2005 John Wiley & Sons, Ltd.     

Comparing the Chronological Distribution of Enamel Hypoplasia in Rogowo,Poland (2nd century ad) Using Two Methods of Defect Timing Estimation

The frequency and chronological distribution of enamel hypoplasias were assessed in a Roman Period population of the Wielbark culture from Rogowo, northern Poland, dated to the 2nd century ad . Hypoplasias were recorded on permanent incisors, canines, and premolars of 52 skeletons. The position of linear defects on the crown surface was measured and then converted to the age of occurrence using two methods: a conventional method that employs the chart of enamel development for the permanent teeth, constructed by Massler et al. (1941) in Swärdstedt's (1966) modification, and the method by Goodman & Song (1999), which corrects for hidden cuspal enamel. Hypoplastic defects were found in 48.1% of examined cases. Linear defects [linear enamel hypoplasias (LEH)] were observed in 38.5% of individuals and in 22% of investigated teeth. The chronological distribution of LEH according to the conventional method revealed two peaks of defects: one at 2.6–3.0 years of age and the other at 4.1–4.5 years of age. The method that accounts for hidden cuspal enamel also provided two peaks, but they occurred at later ages: 3.0–4.0 and 4.6–5.0 years of age. The prevalence of hypoplasia in the Rogowo population in comparison with other European populations of the Roman Period seems to be rather low for both individuals and teeth affected. This may indicate advantageous living conditions, which are supported by archaeological data that suggest general well‐being of the Wielbark people. Copyright © 2011 John Wiley & Sons, Ltd.     

Enamel hypoplasia in Danish and Lithuanian Late Medieval/Early Modern samples: a possible reflection of child morbidity and mortality patterns

Numerous studies and various interpretations of the prevalence of linear enamel hypoplasias (LEH) based on populations living in different cultural and economic conditions are causing some confusion and raising doubts about whether or not such LEH are reliable indicators of life conditions. An analysis of LEH prevalence patterns was performed on the adults of three populations: Tirup—a Danish Medieval rural population of the 12–14th centuries (131 individuals), Subačiaus str. in Vilnius—a Lithuanian Late Medieval urban population of the 16–17th centuries (88 individuals), and the aristocracy—a Lithuanian pooled sample population from several churches of the 15–18th centuries (66 individuals). Statistical testing of the influence of population affinity, sex and age at death was also performed. Since the same investigator scored all the samples, possible inter‐observer error bias was minimized. In all the populations, LEH formation ages were similar. Statistically significant sex differences were found only in the aristocratic sample (with higher male frequency). It was found that the town population was characterized by the highest frequency of affected individuals, with the highest severity and highest number of stress episodes per individual. A reliable relationship with age at death was found only in the town sample: individuals with more numerous and more marked LEH had shorter life expectancies in adulthood. Different “most vulnerable years” for subsequent life expectancies were noted for different populations. A tentative explanation of these differences could be the pooled effect of differential morbidity and mortality. It seems that the rural population experienced the highest morbidity and nutrition deficiency as well as the highest child mortality whereas, in the urban sample, high morbidity was accompanied by a lower child mortality. Both lower morbidity and mortality seem to be characteristic for the aristocracy. Copyright © 2002 John Wiley & Sons, Ltd.     

Diet and Oral Health of Populations that Inhabited Central Argentina (Córdoba Province) during Late Holocene

The aim of this work is to study, from a bioarchaeological perspective, the diet and oral health of the populations that inhabited Central Argentina in two periods which would reflect changes in subsistence strategies: earlier late Holocene (ca. 2500–1500 years BP) and later late Holocene (ca.1500–400 years BP). The sample is composed by 83 adult individuals from 47 archaeological sites. We considered five non‐specific indicators of stress, infectious and degenerative diseases: hypoplasia of dental enamel, dental caries, abscesses, antemortem teeth loss, and calculus. We also considered hypercementosis, dens in dens, and agenesis. We test intraobserver error by means of intraclass correlation coefficient and analysis of variance of repeated measures. We calculated prevalence by sex, age, geographic subregion, and chronological period. We applied Chi‐square (X²) to test statistical significance of observed differences. Considering the sample as a whole, low prevalence of dental caries (10.27%), abscesses (16.52%), and hypoplasia (10.84%) are coincident with values observed for populations with mixed or hunter‐gathering subsistence strategy. In later late Holocene, high incidence of caries should be considered as indicator of consumption of C4 vegetables or other carbohydrate‐rich vegetables, such as legumes of Prosopis sp. Also, moderate values of dental enamel hypoplasia should be related with metabolic‐systemic stress episodes. Summarizing, these results are coincident with isotopic, archaeological, and ethnohistorical evidences which suggest climatic, social, and demographic pressures that might have affected the lifestyle of these populations before the Spanish conquest. Copyright © 2012 John Wiley & Sons, Ltd.     

Reconstructing dietary and environmental history from enamel isotopic analysis: time resolution of intra‐tooth sequential sampling

The carbon and oxygen isotopic composition of the mineral phase of tooth enamel is linked to diet and environment. Enamel is not remodelled once formed. Several studies of intra‐tooth isotopic variability in hypsodont mammal teeth have involved sequential sampling of enamel in order to detect changes in diet and environment during tooth formation. The possibility of exploring individual history opens the door to many applications, particularly in palaeoenvironment and herd management reconstruction. However, previous histological investigations have shown that enamel mineralization is a progressive and discontinuous process. The goal of the present study is to determine if this pattern significantly influences the time resolution of an intra‐tooth sequential sampling. Isotopic analyses (δ¹³C) were performed on tooth enamel from steers (Bos taurus) that had changed from a C₃ plant‐based diet to a C₄ plant‐based diet with very different carbon isotopic compositions. The change of diet was reflected in the mineral phase of enamel. The pattern of intra‐tooth isotopic variation suggests that completion of enamel mineralization required six to seven months. Such a lag in enamel mineralization will decrease the time resolution of enamel sequential sampling. The effects of prolonged mineralization of enamel have to be considered when interpreting patterns of intra‐tooth isotopic variations. Copyright © 2002 John Wiley & Sons, Ltd.     

Linear Enamel Hypoplasia and Age‐at‐Death at Medieval (11th–16th Centuries) St. Gregory's Priory and Cemetery,Canterbury, UK

Linear enamel hypoplasia (LEH) is a macroscopically detectable band‐like dental defect, which represents localized decrease in enamel thickness caused by some form of disruption to a child's health. Such dental deformations are utilized in osteoarchaeological research as permanent markers of childhood physiological stress and have been extensively studied in numerous ancient human populations. However, currently there is no such data for medieval populations from Canterbury, UK. Here, LEH is examined in the context of age‐at‐death in human burials from the medieval St. Gregory's Priory and adjacent cemetery (11^th–16^th centuries), Canterbury, UK. The cemetery and Priory burials represented lower (n = 30) and higher status (n = 19) social groups, respectively. Linear enamel hypoplastic defects were counted on mandibular and maxillary anterior permanent teeth (n = 374). The age and sex of each skeleton were estimated using standard methods. Differences in LEH counts, age‐at‐death, and LEH formation ages were sought between the two social groups. Results indicate significantly greater frequencies of LEH in the Cemetery (mean = 17.6) compared to the Priory (mean = 7.9; t = −3.03, df = 46, p = 0.002). Adult age‐at‐death was also significantly lower in the Cemetery (mean = 39.8 years) compared to the Priory burials (mean = 44.1 years; t = 2.275, df = 47, p = 0.013). Hypoplasia formation ages differed significantly between the Priory (mean = 2.49 years) and Cemetery (mean = 3.22 years; t = 2.076; df = 47; p = 0.034) individuals. Results indicate that childhood stress may reflect adult mortality in this sample, and that the wellbeing of individuals from diverse social backgrounds can be successfully assessed using LEH analyses. Results are discussed in terms of the multifactorial etiology of LEH, as well as weaning‐related LEH formation. Copyright © 2012 John Wiley & Sons, Ltd.     

Health Consequences of European Contact in the Great Plains: A Comparison of Systemic Stress Levels in Pre‐ and Post‐Contact Arikara Populations

The biocultural effects of European contact varied considerably throughout the Americas. Some populations were decimated by colonialism, while others benefited from trade relationships and access to new technologies. It has been suggested that initial contact with European fur traders and explorers was economically favourable for Plains village populations, thereby facilitating a period of cultural florescence. In this study, we evaluate the hypothesis that Plains groups were better off than their predecessors during the initial contact period by comparing frequencies of enamel hypoplasia, cribra orbitalia, and porotic hyperostosis in pre‐ and post‐contact Arikara skeletal assemblages. We included both juveniles and adults in our sample to capture variation in the stress experience of different segments of the population. Our analysis revealed similarly low frequencies of cribra orbitalia in the pre‐ and post‐contact groups and no observed cases of porotic hyperostosis. Enamel hypoplasia, however, was significantly more prevalent among post‐contact Arikara villagers when compared to their pre‐contact counterparts, which suggests that stress levels were higher after European contact. Additionally, enamel hypoplasia was most common among post‐contact juveniles. The elevated frequency of enamel hypoplasia in the post‐contact sample combined with the low frequencies of cribra orbitalia and porotic hyperostosis suggests that disease stress, rather than nutritional deficiencies, was likely responsible for the decline in overall health following European contact. These results also suggest that juveniles are sensitive indicators of systemic stress within a population and should be included in bioarchaeological analyses of prehistoric health when available. Copyright © 2015 John Wiley & Sons, Ltd.     

Using plant microfossils from dental calculus to recover human diet: a case study from Tell al-Raqā'i,Syria

Dietary reconstructions based on plant microfossils, such as starch grains and phytoliths, have been useful in increasing our understanding of past human populations. Microfossils have been recovered from sediments, stone tools, and, more recently, dental calculus. Methods for recovering microfossils from dental calculus have yet to be firmly established and there is some question about potential damage to the teeth. Using a sample of teeth from the middle Holocene site of Tell al-Raqā'i, Syria, we tested using a dental pick to sample the calculus. ESEM images taken before and after sampling show no damage to the enamel surface, and examination of the recovered microfossils show that this method provides ample material for study, even when not all of the calculus is removed from the tooth. Preliminary identification of the plant microfossils suggests that these individuals were consuming a variety of plant foods, but that domesticated cereals such as wheat and barley made up a surprisingly small portion of their diet.     

Pig domestication and husbandry practices in the middle Neolithic of the Wei River Valley,northwest China: evidence from linear enamel hypoplasia

Linear enamel hypoplasia is a physical deficiency occurring during the process of enamel formation and it is visible on the crowns of mammal teeth. It can be used to trace physical stresses during life. In this study, linear enamel hypoplasia in samples of pigs from three Neolithic sites in the Wei River valley (China) were recorded and analysed, to investigate the status of pigs at these sites, their living conditions, and furthermore to explore husbandry strategies for pigs during the Neolithic in the valley. The results suggest a domesticated status for pigs in the Neolithic and early Bronze Age. Also the frequency of LEH occurrence for the pigs corroborates a presumed intensification processes of pig husbandry, and the presence of environmental fluctuations during the period are considered. Some evidence also points to the fact that for pig, spring farrowing was the most common reproductive strategy in the Neolithic and early Bronze Age in this region.     

Diet,nutritional status and oral health in hunter‐gatherers from the central‐northern coast of Patagonia and the Chubut river lower valley,Argentina

Archaeological research in central‐northern Patagonia (Atlantic coast and lower the valley of Chubut river) showed that this area was used since at least the Middle Holocene. Stable isotope analyses (¹³C and ¹⁵N) of human bone samples indicate that hunter‐gatherers living in that area had a terrestrial‐marine diet including guanaco meat, land plants, mollusks and pinnipeds. Despite this general trend, intersite variability and changes through time were noted, especially after the late Holocene. These results have been reinforced by archaeofaunal, technological and bioarchaeological records. In this paper, three hypotheses are examined: (a) the diet of these populations was complete and rich enough to ensure good health status and avoid nutritional deficiencies; (b) carbohydrate consumption increased progressively after 1000 BP, when pottery technology was adopted and (c) this kind of mixed diet would have been qualitatively more nutritious than that of other populations of the region, which would have resulted in better nutritional and healthy conditions. These three hypotheses are compared with dental results obtained from 563 permanent teeth from 45 individuals (34 adults and 11 juveniles from both sexes), rescued from burial sites. Indicators of oral health were assessed through the observation of caries, abscesses, wear, pulpar cavity exposure and ante mortem loss. Features of nutritional status such as enamel hypoplasia, porotic hyperostosis and cribra orbitalia were also examined. Given the availability of direct radiocarbon dating for most of the sample, three temporal series were determined: ‘Before 1000 BP’, ‘1000–5000 BP’ and ‘Post‐contact’. No evidence of alimentary stress or iron deficiency was found in individuals from the three series, which accounts for healthy and good nutritional life conditions. After 1000 BP, the results show a progressive increase in the caries percentage and a decrease in abscesses, dental wear and ante mortem losses frequency. This is possibly related to more consumption of processed foods in the last 1000 years. These results were compared with similar studies based on samples from different environments and latitudes of Patagonia. Evidence suggests that mixed diets (marine‐terrestrial) would have been more appropriate and nutritionally complete than exclusively marine or terrestrial diets. Copyright © 2010 John Wiley & Sons, Ltd.     

Evaluating sources of variation in the identification of linear hypoplastic defects of enamel: a new quantified method

Assessment and interpretation of the presence of developmental defects of enamel formation that result from childhood experiences of growth disruption, enamel hypoplasia, is a standard part of almost any systematic bioarchaeological assessment. However, different identification methods lead to different results. This study presents a new method of defect identification that offers a quantitative criterion for establishing defect presence rather than relying solely on observer judgement. It then compares this with a standard field approach to assessing enamel hypoplasia and a non-quantified microscopic approach in order to determine (a) if traditional methods of identifying enamel hypoplasia under little or no magnification offer the same information about the experience of childhood health as methods that examine dental enamel growth microscopically, and (b) the effect of using a quantified criterion to identify enamel hypoplasia. A case study using simultaneously developing teeth in one individual is presented where observations of defect presence are made both macroscopically and microscopically and with and without this novel metric method. The results demonstrate that the chosen scale of observation is a critical variable in identifying linear hypoplastic defects of enamel. Defects that occur in the more cervical regions of a tooth are ‘missed’ by methods that rely on macroscopic observation or the unaided visual judgement of the observer. This finding has considerable implications for the interpretation of reported enamel hypoplasia prevalence.     

Reconstructing patterns of systemic stress in a Jomon period subadult using incremental microstructures of enamel

This study reconstructs systemic stress patterning using incremental microstructures of enamel in a subadult from the Yoshigo cemetery (3200 through 2800 BP), Aichi Prefecture, Japan, with the goal of providing an improved methodological and theoretical framework for interpreting systemic stress among Jomon people. High resolution impressions and replicas were collected for each anterior maxillary tooth. Each replica was studied under 50, 100, or 200× magnification on a measuring microscope. Perikymata spacing and enamel surface profiles were recorded using specialized software. Linear enamel hypoplasia (LEH) defects were identified as accentuated spacing in perikymata combined with a depression in the enamel surface. Each defect was placed within a chronological sequence based on decile location. Stress duration was estimated by counting the perikymata in the occlusal wall of each defect and multiplying that value by the modal periodicity for striae formation. Percentage of enamel growth disruption was estimated by dividing the number of perikymata within the occlusal wall of each matched defect by the total number of perikymata within the imbricational enamel. Eight LEH defects were chronologically matched. Chronology of LEH defects ranged between 1.2 through 3.5 years. Number of perikymata located within the occlusal wall of each defect ranged from one through 20. Estimated durations of stress ranged from 12 through 129.6 days. These durations overlapped with Neandertals, though Point Hope foragers had greater durations of stress with no overlap in interquartile ranges. Disrupted enamel growth ranged from 8.1% in the maxillary left canine to 50.7% in the maxillary right central incisor.     

A protocol for recording linear enamel hypoplasia on archaeological pig teeth

A method is proposed for recording linear enamel hypoplasia (LEH) present on archaeological pig teeth. The methods described have been developed and tested on material from five archaeological sites of a wide range of periods; Durrington Walls (UK), Wellin, Ename, Sugny and Londerzeel (Belgium). Recommendations are made on what teeth, surface, cusp, and side to record, as well as on details of the visibility, the identification and the measurement of LEH lines. Problems encountered with the application of the recording protocol to the five case studies are detailed, and possible interpretative drawbacks are discussed. © 1998 John Wiley & Sons, Ltd.     

Child Health in Five Early Medieval Irish Sites: A Multidisciplinary Approach

With the aim to reconstruct child health in five early medieval (5th–12th c. CE) Irish sites, an osteoarchaeological study of three biological stress indicators – cribra orbitalia, linear enamel hypoplasia (LEH) and periostitis – has been conducted on 229 skeletons. In addition, Irish early medieval written sources testifying on child health during this period were consulted. These data were furthermore combined with the results of stable isotope analyses (nitrogen and carbon) conducted on subadult bone specimens. Cribra orbitalia was found in 27.5% of the studied individuals (48.6% of subadults were affected), with only two cases active at the time of death. The prevalence of LEH per individual is 63.5% (78.9% in subadults and 59.7% in adults). The age ranges of LEH formation vary between 1.9 and 4.8 years for the maxillary teeth and between 1.8 and 6.2 years for the mandibular teeth. Periosteal inflammations were recorded in over one third of the studied subadults (36.2%) with six cases active at the time of death. The stable isotope results suggest a diet based on terrestrial food sources, with little or no marine input. The presented data strongly suggest that most of the individuals were exposed to a high level of physiological stress during their childhood, and as such does not support the so called ‘osteological paradox’ hypothesis. The observed disturbances were probably caused by a synergistic effect of various biological and socio‐cultural factors. Although the historical records indicate certain differences in diet and lifestyle between social classes and the sexes, this study showed that the children of all ages had poor health in all social classes across a wide geographical location for the full time period of the early medieval. Copyright © 2016 John Wiley & Sons, Ltd.     

Stress near the start of life? Localised enamel hypoplasia of the primary canine in late prehistoric mainland Southeast Asia

Localised hypoplasia of the primary canine (LHPC) is characterised by roughly circular defective areas of thinned or missing enamel on the labial surface. This defect is rarely reported in bioarchaeological research. Using samples from late prehistoric mainland Southeast Asia, this paper documents the prevalence of LHPC to produce baseline data for this defect. The samples are from seven archaeological sites in Thailand and collectively span from ca. 4000 to 1500 BP. In the combined samples, 32/79 (40.5%) of individuals and 47/199 (23.6%) of teeth had LHPC. The high occurrence of LHPC may suggest there was poor maternal and infant health. There is also a high occurrence of caries associated with LHPC, which has implications for the assessment of deciduous dental health. This paper stresses the importance of the collection of dental enamel defect data from deciduous teeth including LHPC in bioarchaeological research.     

Distribution of dental caries in an early 17th century swedish population with special reference to diet

The distribution of dental caries was determined in complete and partial human dentitions from a 17th century (1621–1640) city graveyard excavated in Gothenburg, Sweden. Sixty‐three adults and two children, divided into five different age groups, were studied. Altogether 949 teeth (943 permanent and six deciduous) were examined macroscopically using a dental probe and X‐rays. A high number of teeth had been lost post‐mortem. An increase in ante‐mortem tooth loss was found with increasing age. Sixty per cent of all individuals and 12% of all teeth showed signs of caries. The number of carious teeth per subject increased with increasing age. The highest prevalence of individuals with caries was found for the age group 26–35 (69%). Caries were most prevalent in the first, second and third lower molars (60%), while the incisors and canines in the upper and lower jaws were the least affected teeth (1%). The occlusal surface was the area most susceptible to caries (45%), followed by the buccal cemento–enamel junction (16%) and the approximal contact point (11%). This study shows that, although consuming a diet believed to have been lower in sugar content compared to modern populations, caries did affect a rather high number of individuals living in Sweden during the early 17th century. Copyright © 1999 John Wiley & Sons, Ltd.     

Tooth wear in two ancient populations of the Khazar Kaganat region in the Ukraine

Tooth wear is a common phenomenon in archaeological material. It has been related to the abrasiveness of diet and to the tribological attrition of teeth of individuals. Numerous investigations have been carried out in north and middle American samples as well as African anthropological material. Not much is known about tooth wear in European cultures. Eleven skulls from Chervona Gusarovka, and 14 skulls from the Upper Saltov sites of the Khazar Kaganat region (8th–10th centuries AD) in eastern Ukraine, with different diets were examined. A total of 208 teeth were studied for tooth wear, caries prevalence and periodontal status. Abrasion grades were determined according to a standardised classification and statistically evaluated. Periodontal status was measured using the distance between the enamel‐cementum junction and alveolar crest and the gingival attachment level respectively. Tooth wear was significantly different (P < 0.01) between the two populations. A low caries prevalence of 4.2% in the Chervona Gusarovka population and 1.7% in the Upper Saltov population was found. Significantly more alveolar crest bone resorption on the lingual side was found in the premolars and anterior teeth of the Chervona Gusarovka population. No significant differences were found regarding gingival attachment levels and gingival recession. It is concluded that the content and mode of food preparation influenced tooth wear, as reflected by the prevalence of dental caries and periodontal diseases in these ancient populations. Copyright © 2006 John Wiley & Sons, Ltd.     

Recording dental caries in archaeological human remains

Dental caries is an important condition to record in archaeological collections, but the way in which recording is carried out has a large effect on the way in which the results can be interpreted. In living populations, dental caries is a disease that shows a strong relationship with age. Both the nature of carious lesions and their frequency change with successive age groups from childhood to elderly adulthood. There is also a progression in the particular teeth in the dentition which are most commonly affected and, in general, the molars and premolars are involved much more frequently than the canines and incisors. Lower teeth are usually affected more than upper, although the condition usually involves the right and left sides fairly equally. In the high tooth wear rate populations represented by many archaeological and museum collections, there is a complex relationship between the form of lesions and the state of wear, which adds yet another range of factors to the changing pattern of caries with increasing age. In the same populations, chipping, fracture and anomalous abrasion of teeth are also common, and these contribute similarly to the distribution and forms of carious lesion observed. Amongst the living, the pattern of ante‐mortem tooth loss is important in understanding caries and, in archaeological material, there is also the complicating factor of post‐mortem tooth loss. Finally, there is the question of diagnosis. There are diagnostic problems even in epidemiological studies of living patients and, for archaeological specimens, diagenetic change and the variable preservation of different parts of the dentition add further complications. For all these reasons, it is difficult to define any one general index of dental caries to represent the complete dentition of each individual, which would be universally suitable for studying a full range of collections from archaeological sites or museums. Variation in the nature of collections, their preservation, tooth wear, and ante‐mortem and post‐mortem tooth loss mean that when such a general index appears to differ between sites, there could be many other reasons for this, in addition to any genuine differences in caries incidence and pattern that might have been present. It is suggested here that the best approach is instead to make comparisons separately for each tooth type, age group, sex, lesion type and potential lesion site on the tooth. Copyright © 2001 John Wiley & Sons, Ltd.