A Comparison of Two Methods for Recording Entheseal Change on a Post‐Medieval Urban Skeletal Collection from Aalst (Belgium)

This research compares two current methods for recording bony changes at muscle attachment sites, called entheseal changes (EC); the Mariotti method and the Coimbra method, to evaluate the concordance and comparability of results in a post‐medieval skeletal collection from Aalst, Belgium (n = 116). For both methods, the EC scores produce broadly similar patterns, are symmetrical and differ between age groups. Statistical differences between the upper and lower limb and the lower limb of males and females only occur in the Mariotti method. With careful consideration of the influence of different EC score ranges, the results from the two methods can generally be compared.     

Entheseal Changes in Free‐Ranging Versus Zoo Reindeer—Observing Activity Status of Reindeer

We evaluate whether entheseal changes (EC) can be used to reconstruct activity among reindeer to study its domestication. Humans influence reindeer activity patterns through corralling, which renders them less mobile than free‐ranging reindeer and through use as draught animals, which can result in overuse of certain muscles. There is also variation within free‐ranging reindeer in baseline activity levels due to environmental factors. Other sources of variation at entheses include age, sex, pathology, size, and genetic disposition. Here we examine if there are differences in the entheses between zoo versus free‐ranging reindeer. We recorded the entheses of reindeer long bones using a modified EC scoring system. Weight effects were accounted for by performing analyses separately for specimens under and over 95 kg, but unfortunately age information was lacking. We found that several flexor muscle insertion sites in the ulna were more developed among free‐ranging reindeer. This difference is likely due to feeding behaviour. The free‐ranging animals were digging for lichen from under the snow whereas the zoo reindeer were fed by their keepers. This difference in entheses, however, was found to be significant only among the heavier reindeer. Among the zoo reindeer, the insertion for Subscapularis muscle was more developed, possibly due to increased time spent immobile with the shoulder‐bracing muscle apparatus activated. This difference was significant among the heavier deer and nearly statistically significant among the lighter weight reindeer. Domesticated Siberian draught reindeer fell between zoo and free‐ranging reindeer in several tibial entheses. According to these results, some forelimb entheses may be useful to study differences in mobility and feeding patterns between groups of reindeer. Copyright © 2014 John Wiley & Sons, Ltd.     

Compensatory bone remodelling in moose: a study of age,sex, and cross‐sectional cortical bone dimensions in moose at Isle Royale National Park

We studied interrelationships among age, sex, and cross‐sectional cortical bone dimensions using quantitative computed tomography (QCT) scans of metatarsal bones of 180 moose (Alces alces) that died in Isle Royale National Park, Michigan. As a large‐bodied quadruped with demanding ecological constraints on movement and behaviour, a moose experiences different weight‐bearing and mechanical stressors than humans, to whom most existing studies of mechanical adaptations of bone pertain. In moose, both sexes showed significant subperiosteal expansion and an increase in medullary area, with an overall increase in cortical bone area over time. Female moose did not exhibit cortical thinning or reduction in cross‐sectional area with age, rather they showed an increase in cortical bone area with periosteal apposition exceeding endosteal resorption, similar to the males. We also found that moose undergo changes in bone geometry through remodelling of bone similar to humans, suggesting a compensatory mechanism for increasing bone strength under conditions of decline in bone mineral density with age. Copyright © 2002 John Wiley & Sons, Ltd.     

Using Enthesis Robusticity to Infer Activity in the Past: A Review

In bioarchaeological studies, entheseal change is commonly used to infer patterns of activity in past populations. This category of skeletal modification includes both pathological changes (enthesopathies) and changes in size, shape and surface complexity (robusticity). Despite the recent impetus to reassess the use of enthesopathies as markers of activity, robusticity has received little critical attention. In this review, we reassess key assumptions that underpin the use of robusticity in activity studies, drawing on anatomical, physiological, biomechanical and sports medicine literature sources. We find that, whilst there is some evidence to support the assumption that variation in enthesis robusticity reflects different activity patterns, little is known about the process through which this variation is produced. Presently, the stimuli (dynamics of muscle use or muscle size) for entheseal adaptation are ill-defined, a situation that limits our interpretive abilities. Consideration of bone functional adaptation principles and of the relationship of entheses to soft tissue also highlights how other factors, such as age, sex and genetic background, may influence enthesis robusticity and obscure activity-related adaptation. Understanding how these factors influence enthesis robusticity helps define how robusticity studies should be controlled, but further research is required to clarify how these factors interact with activity in robusticity development and the precise relationship between activity and robusticity. Ultimately, this review emphasises the complexity of entheseal structures and their morphological development. Any interpretation of activity from enthesis robusticity should be approached with caution, but in some circumstances, the endeavour may be ill-advised.     

Sexual dimorphism in diaphyseal cross‐sectional shape in the medieval Muslim population of Écija,Spain, and Anglo‐Saxon Great Chesterford,UK

Differences in adult male and female activity patterns may influence levels of sexual dimorphism in physical dimensions, including the cross‐sectional shape of long bone diaphyses. Previous studies of archaeological populations have demonstrated significant differences in diaphyseal shape between males and females. In this study, dimorphism in external diaphyseal shape of upper and lower limb bones (reflected in indices of external diaphyseal diameters), and bilateral asymmetry in these indices, were examined in two medieval populations: Muslim Écija (Spain) and Anglo‐Saxon Great Chesterford (UK). Attempts were made to relate observed patterns to documentary and other osteological evidence for differences in male and female activity patterns. While few significant differences in upper limb bone cross‐sectional shape were observed in either population, significant differences in shape were found in the lower limb diaphyses at Écija at the femoral midshaft and tibial foramen and midshaft levels, and at the tibial midshaft for Great Chesterford. Comparison with published data suggests that these differences are marked for Écija, and perhaps fairly high for Great Chesterford compared with other populations with an agriculture‐based economy. This is consistent with documentary and osteological evidence suggesting marked gender differences in behaviour in medieval Muslim Spain. No significant differences in bilateral asymmetry were found, but the effects of small sample size cannot be ruled out. Copyright © 2008 John Wiley & Sons, Ltd.     

Analysis of sexual dimorphism of craniofacial traits using geometric morphometric techniques

This work deals with the assessment of cranial sexual dimorphism in human skeletal samples applying geometric morphometric techniques. The purpose of this research is to apply such techniques to quantitatively describe in craniofacial traits the degree and pattern of shape and size sexual dimorphism. Likewise, we evaluate the precision and accuracy of semilandmark‐based techniques for sex estimation. We employ a sample of 125 adult skulls of known sex from the Coimbra collection. A set of coordinate points was selected to describe glabella, mastoid, frontal and zygomatic processes. The results of intra‐class correlation coefficient (ICC) show excellent intra‐ and inter‐observer agreement (ICC > 0.96) in the location of the coordinates of points employed. The principal component analysis (PCA) performed on shape variables shows a large superposition of both sexes, suggesting a relatively low degree of dimorphism in shape. As a consequence, the average percentages of correct sex estimations based on these variables were of 60.12 and 68.90%, obtained by discriminant analysis with leave‐one‐out cross validation and k‐means clustering respectively. Conversely, when centroid size is included in PCA, females and males exhibit large separation along the first component. The highest values of correct assignment (77.86 and 72.15%) were found using shape–size variables with discriminant and k‐means clustering analysis, indicating that the traits analysed display marked sex differences related to the larger size and more robust features of males. Finally, the advantages of geometric morphometric techniques are discussed. Copyright © 2009 John Wiley & Sons, Ltd.     

Vertebral Compression Fractures: Towards a Standard Scoring Methodology in Paleopathology

Vertebral compression fractures are the most common osteoporotic fractures in postmenopausal women. Notwithstanding, its clinical diagnosis remains ambiguous. In paleopathological studies, vertebral fractures and/or deformations are frequently disregarded. When observed, vertebral compression fractures are usually recorded without the support of quantifiable and comparable protocols. As such, Genant's semi‐quantitative method for vertebral compression fracture assessment was applied to a large sample (N = 196) from the Coimbra Identified Skeletal Collection, Portugal, and the reliability of the method was tested. Vertebral fracture scoring agreement was evaluated with the kappa statistic and the per cent of agreement. Intra‐observer agreement was almost perfect, whereas inter‐observer agreement was substantial to almost perfect. The Genant's semi‐quantitative scoring methodology is easy to apply and highly reproducible; as such, it should be adopted as the standard method to score vertebral fractures in any paleopathological investigation. Copyright © 2014 John Wiley & Sons, Ltd.     

Concurrent assessment of urban environment and cardiometabolic risk over 10 years in a middle‐aged population‐based cohort

Inference regarding the impact of urban areas on health is limited by cross‐sectional studies assessing few dimensions and ignoring area‐level socio‐economic status. This study simultaneously assessed several dimensions of the built environment against incident cardiometabolic risk (CMR) arising over 10 years. It tested the hypothesis that, accounting for local area relative wealth, features of the built environment would not predict incident CMR. Initially, disease‐free adults in a biomedical cohort in Adelaide, Australia, provided address and clinical data over three waves of follow‐up. CMR was defined as the count of five clinical CMR factors. Built environment measures were derived for urban form, and natural, and food environments. Local area wealth was expressed using the relative location factor index. Poisson growth models accounting for within‐suburb clustering, age, sex, and education were used to estimate associations between built environment measures and increasing CMR. Fitted linear trajectories had statistically significant mean values of intercepts and slopes. CMR trajectories were associated with age, male sex, and low education. In models including measures of the food, natural, and urban form environments, per standard deviation increase, only POS count predicted incident CMR, which was more strongly predicted by relative location factor. Not accounting for local area socio‐economic status may overestimate the strength of relationships between health and the built environment. Inequity in accessible POS is robustly related to incident CMR.     

New Method for Sex Prediction Using the Human Non‐Adult Auricular Surface of the Ilium in the Collection of Identified Skeletons of the University of Coimbra

Sex estimation in non‐adult skeletons is crucial in bioarchaeology and forensic anthropology. It was not extensively considered in the past, mainly because it was stated that the dimorphic osteological features were difficult to identify before adulthood. Over the past few years, this statement was disproved, and the study of numerous dimorphic non‐adult skeletal traits was approached. This paper presents a new methodology that evaluates the auricular surface of the non‐adult ilia. Several morphological and continuous variables were recorded for 34 individuals (21 females and 13 males) aged between 7 and 18 from the Coimbra Identified Skeletons Collection (University of Coimbra, Portugal). The results show low intra and inter‐observer errors for all the variables, which renders the methodology replicable. Two ratios related to the shape of the anterior area of the auricular surface offer the most dimorphic data (proportions of cases correctly assigned: 0.82 and 0.88; sexual allocation probabilities: 0.85 for both variables). A discriminant function and a logistic regression were developed, which correctly classified the 82.35 and the 88.23% of the individuals, respectively. Moreover, two qualitative variables, referred to as the overall morphology and the apex morphology , also show statistically significant differences between males and females (proportions of correct assignation: 0.82 and 0.76; sexual allocation probabilities: 0.79 and 0.76). These variables can be incorporated in a multifactorial approach together with other indicators already available in the specialised literature in order to help improve the accuracy of the results obtained. This methodological procedure has to be applied with other identified samples, including younger individuals, so as to test whether the trends presented in this context are maintained and are useful in populations from a different geographical provenience. Copyright © 2017 John Wiley & Sons, Ltd.     

Bone weight: new reference values based on a modern Portuguese identified skeletal collection

Skeletal weight and/or weight of the different bones of the human skeleton are currently used in a wide range of applications such as archaeological cremations and forensics. Still, few reference values are available that compare the mean weights for the different skeletal parts. In this paper we present new reference values for total skeletal weight, including the weight of the different skeletal bones based on a modern Portuguese Identified Skeletal Collection (CEI) curated in the Museum of Anthropology of the University of Coimbra (Portugal). The mean weight of the entire skeleton for the CEI pooled sample is 3323.8 ± 779.6 g. Sex and age differences were investigated. As expected, males display heavier bones, at a statistically significant level. The mean weight of the male skeletons is 3850 g, and 2797.6 g for the female sample. Age differences were found, especially for the female samples in the 29–39 versus 50–59 and/or >60 age groups, probably as a consequence of age‐related bone loss in post‐menopausal women. For the male sample, no clear age‐related trend was observed but for the unexpected result that the second highest bone weight recorded is in the oldest age group (>60 years). This could result from high mechanical loadings and thus greater bone robusticy and amount of cortical bone. The percentage of weight of the different parts of the skeleton was also calculated. These new values can be quite useful in the study of very fragmentary and/or commingled human remains, recovered from historic or forensic contexts, since the complete identification, by side, of the remains is not necessary. Copyright © 2008 John Wiley & Sons, Ltd.     

The Effect of Terrain on Entheseal Changes in the Lower Limbs

One of the main factors involved in entheseal changes (EC) aetiology may be related to the physiological limits of biomechanical loading fixed during bone development, such that higher load during childhood and the adolescent growth spurt leads to a lower frequency of EC during adulthood. In this sense, it is possible that ECs may be related to overloading beyond an individual's normal physiological limits as established during childhood and adolescence. This meta‐analysis tested this aetiological possibility by studying the influence of terrain on the entheses of the lower extremities. The hypothesis is that individuals who inhabited rugged terrain have lower EC than those living in flat terrain. This is because biomechanical loads associated with rugged terrain will lead to a higher normal capacity (defined during skeletal development) mitigating the probability of overloading compared to those living in flat terrain who will therefore have a higher frequency of ECs. To test this, papers reporting EC frequencies in the lower limbs were analysed alongside the local terrain. Terrain was defined into two categories: flat or rugged based on altimetry profile, i.e. the average elevation gains and losses along four specific paths (North‐South, East‐West, Northwest‐Southeast, Southwest‐Northeast). Odds ratios were calculated to compare rugged and flat terrain. The overall results are consistent with the hypothesis that overloading is a factor in EC aetiology. However, when the analysis is conducted by sex and side, this general trend does not always occur. Limitations such as the lack of standardized age ranges could be affecting the outcome, i.e. older individuals have a higher frequency of ECs. The findings of this analysis suggest that the theoretical assumptions associated with the cause of ECs require further testing and evaluation. Copyright © 2017 John Wiley & Sons, Ltd.     

Bioarchaeological Analysis of a Royal Burial from the Oldest Maya Tomb in Palenque,Mexico

In 1957, a new discovery inside Temple XVIII‐A was made in Palenque, the Mayan archaeological site in Chiapas Mexico, a royal tomb of an original design which proved to be the oldest at the site. Two skeletons were found, the principal (PAL‐44) was at the centre showing red pigmentation on the surface of some bones, personal adornments and offerings, which indicate that he was an early ruler of Palenque. The second (PAL‐45), deposited at the entrance, not in a special mortuary position, without offerings, suggests it was of a lower social status. This research presents a new bioarchaeological analysis with the following results: (i) The result of a direct radiocarbon dating of PAL‐44 by accelerator mass spectrometry is 250–420 cal AD (LTL12759A:1696 ± 30 BP). This means that the personage was buried long before the first designated ruler of Palenque, who, according to the epigraphic record was enthroned in ad 431. (ii) With X‐Ray Fluorescence, the red pigment was identified as cinnabar (HgS). (iii) PAL‐44 is a young adult male with a severe idiopathic scoliosis with a double curvature that affected the ribcage and lower limbs. Such a three‐dimensional deformity resulted in changes to his body mechanics and induced motion asymmetry. Consequently, his health and mobility must have been significantly affected by such a severe condition. (iv) PAL‐45 is an adult female with signs of nutritional problems in childhood and entheseal changes probably related to her activity. Three ante‐mortem traumas are registered: rib fracture, frontal depressed circular fracture and a nasal septal deviation. This traumatic event took place two weeks, approximately, prior to PAL‐45's death. Copyright © 2013 John Wiley & Sons, Ltd.     

Bone remodelling following a lower leg fracture in the 11,000‐year‐old hunter‐gatherer from Vado all' Arancio (Italy)

The Upper Palaeolithic skeleton from Vado all'Arancio (Italy), dated to about 11,330 BP, exhibits a severe ankle fracture healed with residual deformation. Following recovery, this young hunter‐gatherer continued to walk for an extended period of time, albeit in a mechanically altered manner. While right‐left differences in external lower limb bone measurements are relatively low, biomechanical analysis of femur and tibia indicates unusually pronounced asymmetry in all cross‐sectional measures of diaphyseal strength. Asymmetry results primarily from normal side endosteal hypertrophy, and not from hypotrophy of the injured limb, suggesting that this individual resumed active life following recovery. This pattern of asymmetry underscores the role of physical activity in maintaining bone mass. Copyright © 2002 John Wiley & Sons, Ltd.     

Comparison of coordinate measurement precision of different landmark types on human crania using a 3D laser scanner and a 3D digitiser: Implications for applications of digital morphometrics

A total of 11 340 Cartesian coordinates of 42 homologous landmarks on five excavated human crania were recorded by three observers using a three‐dimensional (3D) digitiser and computer models created with a 3D laser scanner. The aim was to compare the errors of the coordinate data of landmarks of different types recorded with these two techniques. The results showed that digitiser‐based and 3D model‐based coordinate measurements had overall standard deviations of, respectively, ±0.79 and ±1.05 mm. However, the 3D digitiser yielded the most precise coordinate data for landmarks defined primarily by biological criteria (Type I landmarks), while the 3D laser scanner models yielded the most precise coordinate data for landmarks defined primarily by geometric criteria (Type III landmarks). These findings are likely to influence the research design of future craniometric studies, as they indicate that the suitability of certain landmark types as reference points for geometric operations, such as partial Procrustes analysis, depends on the method by which they are measured. This information is particularly important for retrospective research or for combined databases such as FORDISC or CRANID, which may integrate different types of landmarks recorded by different researchers and/or instruments. Crania displaying poor preservation and surface discoloration yielded larger measurement errors, especially for the 3D model measurements. This is not surprising given that landmarks on 3D models cannot be located using tactile means, but have to be located solely on a visual basis. Nonetheless, even though the digitiser measurements exhibit an overall precision slightly greater than the 3D model measurements, both techniques yield coordinate data with a precision sufficient for most craniometric research. Copyright © 2010 John Wiley & Sons, Ltd.     

Skeletal and Surgical Evidence for Acute Osteomyelitis in Non‐Adult Individuals

Osteomyelitis is a non‐specific infection of the bone and bone marrow. In the past acute osteomyelitis (AO) led to high mortality especially in non‐adults. Nevertheless, its diagnosis in archaeological populations is rare. Documented individuals with known cause of death offer a unique opportunity to study this condition. This article aims to describe the bone lesions in non‐adults diagnosed with AO at the Coimbra University Hospital (CUH) and now belonging to the Coimbra Identified Skeletal Collection (CISC). Moreover, mortality rates and demographic profiles for individuals aged ≤18 years old and diagnosed with AO in the CUH, between 1923 and 1929, were also determined. The 5 (1%) non‐adults in the CISC with AO listed as cause of death were selected for this study, and their bones were analysed macroscopically and radiologically. The skeletal remains of one individual revealed a small area of new bone formation in the shaft of the left femur. Radiography of this bone showed a radio‐opaque area in the diaphysis. The other four individuals show evidence of surgical treatment, responsible for many cases of disability in the past. In the 7‐year period under analysis, 122 juveniles were diagnosed with osteomyelitis and admitted for surgery at the CUH, 43 (35.2%) of which were diagnosed with AO. Sixty‐five per cent of the cases of AO occurred between the ages of 8 and 15 years, with boys twice as frequently affected as girls, and lower limb bones were involved in 91% of AO cases. AO was responsible for 76.9% of the deaths. This study of pre‐antibiotic non‐adults may help to recognize skeletal signs of AO and their surgical treatment in unidentified skeletal remains. Copyright © 2012 John Wiley & Sons, Ltd.     

Vascular Grooves on Human Femora and Tibiae as a Potential Activity‐related Trait

The presence of vascular grooves on the shafts of femora and tibiae was scored for a sample of human remains from a mass burial at Tell Majnuna, Syria, dated to the early fourth millennium bce . In the sample of 140 femora and 64 tibiae, many of which were damaged or fragmented, the grooves were most common on the femoral midshaft (31/108 = 28.7%) and on the proximal and middle shaft of the tibia (10/45 = 22.2 and 14/54 = 26.0%, respectively). For femoral midshafts, the difference in mean cross‐sectional size and shape indices between bones with and without vascular grooves was checked with multivariate analysis of variance for a sample of 51 bones. The presence of grooves appeared to correspond significantly with higher mean values of both indices. As the shape index is interpreted as an indicator of the level of terrestrial mobility and the size index may be used for sex assessment, it is possible that vascular grooves occur more frequently at the femoral midshafts of men and more active individuals, and thus they may be cautiously interpreted as another activity‐related trait. Copyright © 2012 John Wiley & Sons, Ltd.     

Enthesopathies and activity patterns in the Early Medieval Great Moravian population: Evidence of division of labour

Bone remodelling in the areas of entheses is frequently supposed to be associated with physical stress and the activity patterns of ancient populations. The main aim of this study was to analyse the prevalence of enthesopathies among individuals living in different life conditions. A total of 197 individuals from the Early Medieval (9th century) Mikulčice site were analysed for enthesopathies ‐ 117 adult individuals from Mikulčice castle and 80 individuals from its hinterland. While the inhabitants of the castle were probably ranked among the higher echelons of society, the hinterland was predominantly inhabited by a farming population. It is hypothesized that individuals from the castle were exposed to lower physical stress than individuals from the hinterland. 9 fibrocartilaginous entheses of the upper and lower limbs were evaluated. Enthesopathies occurred in at least one enthesis in 76% of individuals younger than 50 years of age. Significant differences were observed in the occurrence of enthesopathies between castle and hinterland. Males from the agricultural hinterland show the highest prevalence of enthesopathies of all the population groups, while the lowest prevalence of the marker was recorded in males from the castle. Females in the castle were more affected by entheseal changes than females from the hinterland. Sexual dimorphism was more evident in the hinterland. The present study confirmed presumptions based on the archaeological finds, especially for males. This hypothesis cannot be accepted for females. We may suppose that in the 9th century, women living in Mikulčice castle did not represent a privileged social class. Copyright © 2010 John Wiley & Sons, Ltd.     

Age Changes in the Clavicle: from the Early Neonatal Period to Skeletal Maturity

Of all the long bones in the human skeleton, it is the clavicle which displays the longest period of growth-related activity, rendering it particularly useful for the estimation of age at death in the earlier years. However, because of the universal paucity of documented juvenile remains, most previous studies have, by necessity, not only been based on material of estimated age at death but also restricted to a narrow age range. The aim of the present study was to chart developmental age-related change in the clavicle across its full growth range using juvenile and young adult material of documented age at death. Maximum diaphysial length was recorded for prepubertal individuals and a grading of the progressive alterations in medial epiphysial morphology was recorded for post-pubertal specimens. In this way, age changes in the clavicle were examined across its developmental maturity continuum.     

Changes in the Abundance and Diversity of Proteaceae in South‐western Australia: a Review of an Integrated Palaeoenvironmental Study

South‐western Australia is a globally significant hotspot of Proteaceae diversity. This review reports on changes in the abundance and diversity of Proteaceae in south‐western Australia. Using palynology, the data were obtained from three sediment sequences from the Eocene, Pliocene and Holocene, as well as a modern pollen rain study, in the context of a vegetation history framework. The total percentages of Proteaceae pollen in pollen counts indicate that the number of Proteaceae in the vegetation are highest at present, slightly lower, yet still high in the Eocene, reduced by the Pliocene and lowest in the Holocene. It was found that Proteaceous genera can contribute up to 50% of the total modern pollen rain. Sediment from Lake Lefroy showed Nothofagus‐dominated rainforest occurred in the Middle to Late Eocene. Proteaceae species were at least as diverse as today, contributing up to a maximum of 42% of the total pollen rain, and varying across small lateral distances. A laminated Pliocene age sequence from Yallalie confirmed other studies that south‐western Australia was covered by a rich vegetation mosaic consisting of heath and wet rainforest elements. Proteaceae species were a consistent component of the counts, although diversity and abundance (maximum 5%) were low. A Holocene record from Two Mile Lake, near the Stirling Range, recorded little environmental change. Proteaceae species were noted in low abundance, at a maximum of 3.5% of the total pollen count. It is likely that both changing pollination mechanisms and changes in associated vegetation are important in determining the dispersal of Proteaceous pollen.     

A comparative experiment in the consolidation of cremated bone

This experiment assessed whether consolidation of cremated remains facilitated cross‐sectioning and grinding, which often are required for microstructural analyses such as the histomorphometric estimation of age and the examination of pathological lesions. Green, bovine femoral diaphyses were cremated at three temperature ranges (346–357°C; 610–755°C; 684–838°C) in an electric furnace and then consolidated with Acryloid B‐72 or Butvar B‐98 using simple brushing and immersion techniques. After consolidation was complete, bone pieces were cross‐sectioned and ground to a thickness of 100–120 μm. Results indicate that specimens treated with Acryloid B‐72 are harder and stronger than Butvar treated and untreated control specimens. Acryloid B‐72 is, therefore, recommended for the consolidation of cremated remains in preparation for microstructural analysis. Copyright © 2004 John Wiley & Sons, Ltd.