Identification of historical human skeletal remains: a case study using skeletal and dental age,history and DNA

Excavation of an early historical (circa 1900) church cemetery in Cochrane, Alberta, Canada, revealed the graves of six individuals. All but one had been previously excavated and the individuals were re‐interred elsewhere. The remaining grave contained a coffin burial of an infant including most of the skeleton, crowns of forming deciduous teeth, scalp and hair. The remains were excavated and historical research was carried out to determine possible families who had used the cemetery. Skeletal and dental age estimates pointed to a particular individual described in historical records. This identification was confirmed through more precise ageing by dental microstructure, sex determination using DNA, and finally, maternal relatedness by comparison of mtDNA with a living female relative. In addition to describing the analytical methods used, this paper demonstrates the importance of accurately identifying historical burials, particularly when living relatives remain in the community. Copyright © 2004 John Wiley & Sons, Ltd.     

Linear Enamel Hypoplasia and Age‐at‐Death at Medieval (11th–16th Centuries) St. Gregory's Priory and Cemetery,Canterbury, UK

Linear enamel hypoplasia (LEH) is a macroscopically detectable band‐like dental defect, which represents localized decrease in enamel thickness caused by some form of disruption to a child's health. Such dental deformations are utilized in osteoarchaeological research as permanent markers of childhood physiological stress and have been extensively studied in numerous ancient human populations. However, currently there is no such data for medieval populations from Canterbury, UK. Here, LEH is examined in the context of age‐at‐death in human burials from the medieval St. Gregory's Priory and adjacent cemetery (11^th–16^th centuries), Canterbury, UK. The cemetery and Priory burials represented lower (n = 30) and higher status (n = 19) social groups, respectively. Linear enamel hypoplastic defects were counted on mandibular and maxillary anterior permanent teeth (n = 374). The age and sex of each skeleton were estimated using standard methods. Differences in LEH counts, age‐at‐death, and LEH formation ages were sought between the two social groups. Results indicate significantly greater frequencies of LEH in the Cemetery (mean = 17.6) compared to the Priory (mean = 7.9; t = −3.03, df = 46, p = 0.002). Adult age‐at‐death was also significantly lower in the Cemetery (mean = 39.8 years) compared to the Priory burials (mean = 44.1 years; t = 2.275, df = 47, p = 0.013). Hypoplasia formation ages differed significantly between the Priory (mean = 2.49 years) and Cemetery (mean = 3.22 years; t = 2.076; df = 47; p = 0.034) individuals. Results indicate that childhood stress may reflect adult mortality in this sample, and that the wellbeing of individuals from diverse social backgrounds can be successfully assessed using LEH analyses. Results are discussed in terms of the multifactorial etiology of LEH, as well as weaning‐related LEH formation. Copyright © 2012 John Wiley & Sons, Ltd.     

Dental Health of Free Blacks in New York State during the Mid‐19th Century

The current research will examine the frequency of carious teeth, periapical abscesses and ante‐mortem tooth loss in the Newburgh Colored Burial Ground (1830–1870), a free black cemetery in Newburgh, New York. The Newburgh material is compared with skeletal samples from the New York African Burial Ground, New York State almshouses, a free black cemetery from Philadelphia and middle‐class/upper‐class European cemeteries. Although previous research suggests that dental health became worse throughout the 19th century, there is no consistent pattern between the 17th‐century and 18th‐century skeletal sample of enslaved blacks from New York City and 19th‐century free blacks from Newburgh and Philadelphia. The frequency of dental caries does increase through time but the other indicators change little (equal or fewer ante‐mortem tooth loss) or suggest an improvement in dental health (fewer periapical abscesses) through time. Relative to contemporaneous populations, the individuals from Newburgh appear much more similar in terms of dental health to upper‐class and middle‐class European groups than to the other marginalised groups considered in this research. The impact of the economic, political and social changes that accompanied the Industrial Revolution and their potential impact on dental health are considered. Copyright © 2011 John Wiley & Sons, Ltd.     

Dental Modifications in a Skeletal Sample of Enslaved Africans Found at Lagos (Portugal)

An archaeological intervention in Valle da Gafaria (Lagos, Portugal) allowed the excavation of a deposit of waste dating from the 15^th to 17^th centuries. Among discarded objects, an important amount of human skeletal remains was exhumed (N = 158 individuals). The archaeological and historical context, as well as the morphometric analysis of the skulls, led us to attribute them an African origin. While historical sources document the trade of slaves by the Portuguese since the 15^th century, so far no slave cemetery was excavated in Portugal. The study of their lives and deaths has been accomplished by historical documents. Therefore, this sample provides a unique opportunity to learn more about captive individuals who were brought to Portugal in the modern period. The present work focuses in the intentional dental modifications presented by several of these individuals. A total of 113 subjects have teeth that can be evaluated for the presence of intentional modifications. Of these, 55.8% individuals present dental modifications on their anterior dentition, 42.9% exhibiting modifications on both upper and lower teeth. The incisors were the most frequently modified teeth, followed by the canines. Both men and women as adults and sub‐adults have dental intentional modifications. In most individuals, dental modifications involved the removal of the mesial and distal angles, which is comparable with sub‐Saharan African practices. However, we cannot infer a more specific origin for these slaves only based on dental modification's type and pattern because several ethnic groups modify teeth in the same way. Copyright © 2015 John Wiley & Sons, Ltd.     

Chronic Maxillary Sinusitis in Medieval Sigtuna,Sweden: A Study of Sinus Health and Effects on Bone Preservation

Frequencies of maxillary sinusitis in the population of the medieval town Sigtuna, Sweden, were examined. The first aim was to explore the occurrence of sinus conditions in this urban population, and the second was to investigate the effects of preservation in relation to these changes. The skeletal sample consisted of 274 adolescent and adult individuals buried ca 970–1530 AD. The maxillary sinuses were examined for different types of skeletal change related to sinusitis. The severity and location of these changes were recorded, together with the preservation of the sinus. When the diagnostic criteria for bone preservation from previous studies was used, i.e. the preservation of at least one complete antral floor, the results showed that 97.7% (n = 157) of the individuals were affected, with older individuals showing more severe bone changes. In the entire (i.e. more fragmented) sample, 94.5% (n = 259) showed signs of sinusitis. Again, older individuals showed more severe bone changes, and males were more frequently affected. Also, an increased frequency over time was found. The maxillae were subdivided into groups of bone preservation, and the results showed that there was a significant relation between preservation and the registered presence and severity of sinusitis. The results may be used as a cautionary note against investigating the occurrence of maxillary sinusitis without considering the skeletal preservation factor. There was no correlation between preservation and sex or age groups. This suggests that the differences between younger and older and male and female were not a result of preservation alone and could be considered as trustworthy. Copyright © 2011 John Wiley & Sons, Ltd.     

Biological Kinship,Postmarital Residence and the Emergence of Cemetery Formalisation at Prehistoric Marathon

This paper examines the role of kinship and postmarital residence in the emergence of organised cemeteries during the transition from the Final Neolithic to the Early Bronze Age at Marathon, Attica. Focusing on the cemetery of Tsepi, we performed intra‐cemetery biodistance analysis to test whether biological relatedness structured spatial organisation of tombs, and whether postmarital residence was matrilocal or patrilocal. Dental metric, dental morphological and cranial non‐metric data were collected from 293 individuals and subjected to multivariate analysis (principal component analysis, Euclidean distance ordination via multidimensional scaling), binomial probability calculations, Ripley's K analysis and determinant ratio analysis. Results indicated phenotypic similarity among some tomb co‐interments; however, outliers suggested that within‐tomb burial depended on a variety of factors that could include affinal, fictive or practical kinship. There was strong evidence for phenotypic patterning by tomb row, indicating that cemetery structure at Tsepi was organised according to biological lineages. This was especially evident for females. Male phenotypic variation was higher than that of females, though not significantly so. The results of intra‐cemetery biodistance analysis at Tsepi reveal a complex mortuary programme that emphasised biological kinship within an exogamous and likely matrilocal system of mate exchange. When considered in light of ethnographic evidence, the practice of male exogamy may correlate with the coastal location of the community and the maritime activities that structured the economy during the emergence of social complexity in the region. This paper illustrates the potential of biodistance analyses for elucidating aspects of social life in the Aegean. Copyright © 2017 John Wiley & Sons, Ltd.     

The Non‐Adult Cohort from Le Morne Cemetery,Mauritius: A Snap Shot of Early Life and Death after Abolition

The cemetery of Le Morne in Mauritius dates from the 1830s and is thought to contain the remains of slaves, freed slaves or potentially free Madagascans, which in itself has economic and social implications and makes the cemetery all the more intriguing. During 2010, excavations recovered the remains of 11 individuals, of which six were children. Although a small sample, the burials of the non‐adults show several interesting features. Two neonates were buried contemporaneously and may have been twins, while a late term foetus in a grave with a young woman may represent an incident of coffin birth. This sample has the potential to cast light on the burial practices of the slave/ex‐slave community in Mauritius, about which little is currently known. Copyright © 2012 John Wiley & Sons, Ltd.     

A Case of Early Childhood Caries from Late Roman Ancaster,England

This paper presents a differential diagnosis of pathological changes observed in the deciduous dentition of a child aged 3–4 years, recovered from a Late Roman (c. ad 270–410) cemetery at Ancaster, England. The entire maxillary dentition exhibited areas of missing enamel and erosion of the underlying dentine, which was most pronounced at the lingual surfaces of the anterior teeth, and lingual and occlusal surfaces of the molars. Additionally, the mandibular second molars and mandibular right first molar displayed superficial enamel erosion of the buccal surfaces. Radiography indicated normal enamel density, and no further dental pathology was identified. Conditions considered in a differential diagnosis included amelogenesis imperfecta, fluorosis, perimolysis, xerostomia‐related caries and early childhood caries (ECC). The nature and distribution of lesions implicates ECC – also known as ‘nursing caries’ or ‘baby‐bottle caries’ – as the most probable diagnosis. The potential significance of the findings for studies of infant health and feeding practices in past populations is discussed. Copyright © 2015 John Wiley & Sons, Ltd.     

Reconstruction of Socioeconomic Status in the Medieval (14th–15th Century) Population of Grevenmacher (Luxembourg) Based on Growth,Development and Diet

Numerous historical sources describe many aspects of the estate‐based society in medieval times; the detection of socioeconomic status within populations through skeletal remains is a topic of growing interest in anthropological studies. In medieval times, it was common for members of high social rank to be buried within or next to a church. This was certainly the case in Grevenmacher (Luxembourg), where remains of a church building and an adjacent cemetery from the 13th until the beginning of the 15th century were subjected to archaeological and anthropological analysis. By integrating archaeological and anthropological elements, as well as stable isotope analysis, we documented osteological manifestations as indicators of diet differentials in two subsamples (first group consists out of 56 individuals, second one out of 184 individuals) from the medieval cemetery of Grevenmacher. We could distinguish two subsamples that we assumed as different in their socioeconomic status with regards to the burials' position in the cemetery and burial characteristics. Differences in osteological traits such as bone length, stature and body mass indicated differences in diet between sample groups. To substantiate these outcomes, stable isotopic analysis (δ¹³C, δ¹⁵N) were made; the results displayed a clear separation between the sample groups on the basis of their diet. Therefore, we were able to confirm certain individuals in the medieval population of Grevenmacher in relationship to their socioeconomic status. Copyright © 2017 John Wiley & Sons, Ltd.     

Identification of the Archaeological ‘Invisible Elderly’: An Approach Illustrated with an Anglo‐Saxon Example

The aim of this paper is to present a method to facilitate age‐at‐death estimation of older individuals (generally those aged 50+ years) in a representative cemetery sample. The purpose of disaggregating catch‐all categories, such as 50+ years, is to enable the exploration of the elderly (those in their 50s, 60s, 70s or 80s) in the context of mortuary archaeology, bioarchaeology and/or palaeopathology. The methodological steps include the following: (1) assessment of occlusal tooth wear in an Anglo‐Saxon cemetery sample from Worthy Park, UK; (2) seriation of the sample, from youngest to oldest, based on the degree of tooth wear; (3) selection of an ethnographically derived model (known mortality profile) by which seriated individuals in the Worthy Park sample could be reallocated to more realistic or appropriate age classes; (4) reallocation of individuals in the seriated Worthy Park sample to the model age classes. A Hadza, Tanzania, hunter‐gatherer profile was chosen to model the Worthy Park sample, although others are available. By using this model, some 66% of the entire adult sample, originally allocated to the single final age category of 45+ years, was distributed across four new age categories from the mid‐40s to mid‐70s. Relatively straightforward, this approach provides a way to identify those individuals, 50+ years old, not normally sensitive to traditional age‐at‐death estimation methodologies currently available. Copyright © 2014 John Wiley & Sons, Ltd.     

Nutritional and disease stress of juveniles from the Dakhleh Oasis,Egypt

Bioarchaeologists use skeletal health indicators to measure how ancient populations adapted to their physical, cultural and biological environments. Skeletons of infants and children are rarely included in these kinds of analyses because of factors such as poor preservation, small sample size, incomplete recovery or research design. In this study, skeletal remains of juveniles aged from foetal to 15 years (N = 238) from Kellis 2, a Romano‐Byzantine cemetery in the Dakhleh Oasis, Egypt, are analysed to shed light on how infants and children were affected by Roman policies during the early years of the Christianisation of Egypt. Non‐specific indicators of physiological stress (cribra orbitalia, enamel hypoplasia and osteoperiostitis) are analysed for post‐natal individuals and interpreted in the context of the physical, cultural and biological landscapes. Results from these analyses suggest moderate levels of skeletal and dental stress with a marked improvement in general health from pre‐Roman times. This study contributes to a better understanding of juvenile paleoepidemiology and mortuary practices in Egypt during the Romano‐Byzantine period. Copyright © 2010 John Wiley & Sons, Ltd.     

A Note on the Demographic and Health Pattern of a Historical Sleeping Sickness Cemetery at Letsholathebe Memorial Hospital,Maun, Botswana

A part of a historical cemetery used to bury victims of sleeping sickness in Maun, Botswana, was recently uncovered by a water supply improvement construction project. The cemetery had become obliterated from the surface and from the memories of the people resulting in the use of the land for residential purposes. Twelve skeletons were excavated along the water supply trench and have since been buried at one of the new village cemeteries. There were eight males, two females and two individuals of unknown sex. They were young adults ranging in age from 18 to 35 years at the time of death. Dental modifications were used to estimate the cultural identities of two individuals. Some of the remains had been buried with body clothing such as belts and necklaces. Poor dental health was most probably associated with poor nutrition and poor oral hygiene among the population. Copyright © 2015 John Wiley & Sons, Ltd.     

Dental trauma and antemortem tooth loss in prehistoric Canary Islanders: prevalence and contributing factors

Differential diagnosis of the aetiology of antemortem tooth loss (AMTL) may yield important insights regarding patterns of behaviour in prehistoric peoples. Variation in the consistency of food due to its toughness and to food preparation methods is a primary factor in AMTL, with dental wear or caries a significant precipitating factor. Nutritional deficiency diseases, dental ablation for aesthetic or ritual reasons, and traumatic injury may also contribute to the frequency of AMTL. Systematic observations of dental pathology were conducted on crania and mandibles at the Museo Arqueologico de Tenerife. Observations of AMTL revealed elevated frequencies and remarkable aspects of tooth crown evulsion. This report documents a 9.0% overall rate of AMTL among the ancient inhabitants of the island of Tenerife in the Canary Archipelago. Sex‐specific tooth count rates of AMTL are 9.8% for males and 8.1% for females, and maxillary AMTL rates (10.2%) are higher than mandibular tooth loss rates (7.8%) Dental trauma makes a small but noticeable contribution to tooth loss among the Guanches, especially among males. In several cases of tooth crown evulsion, the dental root was retained in the alveolus, without periapical infection, and alveolar bone was in the initial stages of sequestering the dental root. In Tenerife, antemortem loss of maxillary anterior teeth is consistent with two potential causal factors: (a) accidental falls while traversing volcanic terrain; and (b) interpersonal combat, including traditional wrestling, stick‐fighting and ritual combat. Steep‐walled valleys (barrancos) and lava fields (malpaís) required agile locomotion and occasional vaulting with the aid of a wooden staff. Accidental falls involving facial injury may have contributed to AMTL. Traditional conflict resolution involved competitive wrestling (lucha canaria), stick‐fighting (juego del palo), and ritualised contests involving manual combat. These activities made a small but recognisable impact on anterior dental trauma and tooth loss. Inter‐personal behaviours of such intensity leave their mark on skeletal and dental remains, thereby providing insight into the lives and cultural traditions of the ancient Guanches. Copyright © 2006 John Wiley & Sons, Ltd.     

Monks on the move? An assessment of mobility at the medieval Nubian monastery of Ghazali,Sudan (ca. 680–1,275 CE)

The location of Ghazali monastery away from the Nile valley within the relatively isolated environs of the Bayuda desert presents a landscape suggestive of mobility toward the monastery by those who chose to reside there as monks. To assess this potentiality, a sample of 37 individuals from the monastic cemetery (Cemetery 2) were analysed for ⁸⁷Sr/⁸⁶Sr and δ¹⁸O to assess residency during dental enamel formation. The data generated bring into question the nature of mobility to Ghazali monastery, particularly in regard to the potential movement of people from the Nile valley, adjacent desertic landscapes, and further afield.     

The History and Composition of the Identified Human Skeletal Collection of the Certosa Cemetery (Bologna,Italy, 19th–20th Century)

The purpose of this paper is to present the identified skeletal collection (by age, sex, cause of death, occupation and preservation state) from the Certosa Cemetery of Bologna (Italy), which was reviewed and checked through a consultation of the cemetery archives. The collection consists of 425 skeletons of individuals (from newborn to 91 years old) who died in Bologna between 1898 and 1944. The personal details associated with the skeletons were cross‐checked with the data contained in the cemetery and municipal archives. For each skeleton, the biological profile was assessed using current anthropological methods in order to confirm its correct identification. Four hundred and eighteen skeletons (98.4%), mostly complete and well preserved, are identified at least for sex, and for 95% of these, the age is known. The distribution of sexes in the various age groups is fairly well balanced. The cause of death is known in 93% of the individuals. Approximately 30% of the individuals died from infectious diseases. The occupation is known for more than 92% of the individuals. Most of the women were housewives, while the men were employed in various jobs. The cross‐check between archival data with the anthropological analysis of the remains enabled a reliable identification of the skeletons. The sexes and various age groups are well represented, and the sample is substantially uniform as far as geographical origin and socio‐economic conditions (lower social classes) are concerned. Thanks to the reliability of the information collected, the Certosa collection is an excellent anthropological tool for the development and validation of osteobiographic methods. Copyright © 2017 John Wiley & Sons, Ltd.     

孙家南头墓群周墓地分析

通过对墓地分区和墓葬特征的分析可知,凤翔孙家南头墓群周墓地的墓主至少应由周系族群和广义的殷遗民组成,其中以后者居多。墓地结构反映出这一地区社会亲属关系结构至少分为三个层级。不同亲属集团的墓葬群同处一地,两大族群的文化相互影响。二者人群主体的等级普遍较低,但殷遗民中不乏地位相对稍高者。此外,西周晚期该墓地规模的缩小,很可能与周人对关中西部地区控制力减弱有关。     

Pre‐Columbian Tuberculosis in Northwest Argentina: Skeletal Evidence from Rincón Chico 21 Cemetery

Systematic excavation of collective burial sites makes possible the recovery of skeletal series which may show bony evidence of infectious pathological conditions. This paper presents the first evidence of the existence of tuberculosis in prehistoric populations of NW Argentina with a subsistence economy based on agriculture and pastoralism. The study was carried out on individuals from Rincón Chico 21 cemetery, a burial site located in the Santa María Valley, Catamarca, used between the Late Ceramic Period and the onset of the Inca empire expansion (AD 1000–1400). Six individuals out of the 70 so far excavated showed destructive lesions in the vertebral bodies and periosteal reactions in other bones. The morphology and distribution of bone lesions led us to rule out several diseases from a broad spectrum of possible diseases that could have affected the skeletal system. Thus, the lesions were interpreted as caused by mycobacterial infections (Mycobacterium tuberculosis Complex). Considering previous studies on the dynamics of biocultural interactions which take into account information related from contextual associations and chronology, we can conclude that a tuberculosis‐like disease was present in prehistoric populations from NW Argentina. Copyright © 2011 John Wiley & Sons, Ltd.     

Biomass Spectrometry Identification of the Fibre Material in the Pall Imprint Excavated from Grave M1, Peng‐state Cemetery,Shanxi, China

In order to identify the fibre material of the pall imprint excavated from the Peng‐state cemetery in Shanxi, biomass spectrometry was applied to determine the amino acid sequences of the residual protein extracted from the soil underneath the imprint. The sequences were searched against a standard protein sequence database. A well‐preserved silk pall sample from the Warring States Period was used as a comparative template. The protein extracted is identified as silk fibroin (Bombyx mori). This finding indicates that the extremely degraded pall was made of silk and that the deceased in the Peng‐state cemetery enjoyed high social status. In this way, a novel methodology, which is very promising in uncovering the origin of silk, could be initiated.     

Molar crown height: not always a reliable method for the evaluation of age-at-death

In this study, we tested the validity of molar crown height, which changes according to the degree of tooth wear, for the evaluation of age-at-death. The sample consisted of 372 first and second molars (lower and upper) from 157 individuals of known sex and age-at-death. For each molar, we measured the height of the two cusps most subjected to wear (protocone and hypocone for the upper M1 and M2; protoconid and hypoconid for the lower M1 and M2). The correlation between crown height and age-at-death was assessed by linear regression analysis. The resulting models were not very robust since a significant correlation was only found for a small part of the sample, at best (maxillary M1) around 35%. The result slightly improved when bucco-lingual diameter (BL diameter), ante-mortem tooth loss and dental caries were considered, mainly for the maxillary M2 for which the model using age-at-death and BL diameter as independent variables explained 47% of the sample (p < 0.001).     

Prehistoric dental modification in West Africa – early evidence from Karkarichinkat Nord,Mali

This paper reports the earliest securely dated evidence for intentional dental modification in West Africa. Human remains representing 11 individuals were recovered from the sites of Karkarichikat Nord (KN05) and Karkarichinkat Sud (KS05) in the lower Tilemsi Valley of eastern Mali. The modified anterior maxillary dentitions of four individuals were recovered from KN05. The dental modification involved the removal of the mesial and distal angles of the incisor, as well as the mesial angles of the canines. The modifications did not result from task‐specific wear or trauma, but appear instead to have been produced for aesthetic purposes. All of the filed teeth belonged to probable females, suggesting the possibility of sex‐specific cultural modification. Radiocarbon dates from the site indicate that the remains pertain to the Late Stone Age (ca. 4500–4200 BP). Dental modification has not previously been reported from this region of West Africa and our findings indicate that the practice was more widespread during prehistory. Copyright © 2008 John Wiley & Sons, Ltd.