Integration of use-wear with protein residue analysis – a study of tool use and function in the south Scandinavian Early Neolithic

This study presents research on how an integration of use-wear analysis with protein residue analysis can produce new results on prehistoric tool use and function. Thirty flint artefacts from an Early Neolithic TRB site in south Sweden were analysed for both use-wear and protein residues. The results show a positive correlation between the two methods. The Early Neolithic tools analysed were primarily used to process fish.     

Starch grains analysis of stone knives from Changning site,Qinghai Province,Northwest China

Plant residues recovered from prehistoric stone artifacts can be used to help explain tool function and plant use. At the Changning site in Qinghai Province, Northwest China, dating from 4000 yr BP, we examined starch granules extracted from three slate stone knives. A total of 153 starch grains were retrieved from three stone knives, from which we identified starches from legumes, the Triticeae tribe, foxtail millet (Setaria italica), broomcorn millet (Panicum miliaceum), roots and tubers. These results indicate that the stone knives may have been used for a variety of activities that included reaping grasses and food processing. The species of starch grains retrieved from the study sample reveal that diverse crops were cultivated at the Changning site 4000 years ago.     

Starch residues on museum artefacts: implications for determining tool use

A sample of 11 museum artefacts was examined to assess the preservation of any adhering organic residues, particularly starch granules, and to assess the potential for the identification of cooked starchy foods. The primary aim was to examine starchy deposits, extract starch granules and examine their physical condition to increase our understanding of the taphonomy of starch granules and associated organic residues in archived material and archaeological deposits. The study shows that organic plant residues could be recovered from archived artefacts and makes a significant contribution to understanding the function of this material. Recovered starch granules showed an interesting pattern of varying organic preservation on the surface of stone and wooden artefacts. Some starch granules were found to be well preserved while others in the same sample appeared to have been affected by enzyme degrading micro-organisms. Recovered residues included fully gelatinised starch.     

Technical microwear and residues in identifying bipolar knapping on an anvil: experimental data

The functional study of the stone tool artefacts from the Middle Pleistocene site of Isernia la Pineta (Molise, central Italy) revealed microtraces that display certain features that did not fit in with what we know as use-wear traces. The suspicion that these microtraces may be technical traces derived from bipolar flaking, which is prevalent at this site, led us to initiate an experimental programme to check our hypothesis. The experiments conducted allowed us to identify residues associated with bipolar flaking on an anvil and to characterise the microscopic traces derived from this production technique. Our results proved very useful in identifying the artefacts produced by bipolar flaking, as well as in determining the basic lithological features of the anvil. Moreover, these experiments allowed us to assess the possible interferences that these kinds of technical traces can cause when performing the functional analyses of lithic assemblages produced by the bipolar technique.     

Evaluating the usefulness of phytoliths and starch grains found on survey artifacts

Archaeologists use survey artifacts to study any number of interesting topics. The focus of this study is to test the usefulness of starch grains and phytoliths found on artifacts recovered during archaeological survey. Phytolith and starch grain analysis was used to determine the level of environmental contamination on three types of medieval ceramics collected during survey work from plowed fields in the parish of Wicken, Northamptonshire, England. The plant residues found on these artifacts were compared with their surrounding soil and with contemporaneous excavated artifacts recovered from under the floorboards of a medieval house in nearby Wyton, Cambridgeshire, England. Through the use of the “piggyback” approach pioneered by Chandler-Ezell and Pearsall, phytoliths and starch grains were systematically removed from the artifacts surface. Residues associated with contamination were removed from the outermost layer of the artifact surface while potentially uncontaminated residues were removed from the innermost layer of the artifact surface. Matching phytoliths and starch grains were found on the outermost surface of the survey artifacts and in the surrounding soils. No phytoliths and starch grains were found on the innermost surface of the survey artifacts suggesting that residues from the surrounding soils did not penetrate into the pores and crevices of the artifacts. The overall results suggest that, although they are preliminary, there is potential for plant residue analysis of survey artifacts.     

Potential misidentification of in situ archaeological tool-residues: starch and conidia

Microscopic identification of organic residues in situ on the surface of archaeological artefacts is an established procedure. Where soil components morphologically similar to use-residue types exist within the soil, however, there remains the possibility that these components may be misidentified as authentic residues. The present study investigates common soil components known as conidia, fungal spores which may be mistaken for starch grains. Conidia may exhibit the rotating extinction cross under cross-polarised light commonly diagnostic of starch, and may be morphologically indistinguishable from small starch grains, particularly at the limits of microscope resolution. Conidia were observed on stone and ceramic archaeological artefacts from Honduras, Palau and New Caledonia, as well as experimental artefacts from Papua New Guinea. The findings act as a caution that in situ analysis of residues, and especially of those less than 5 μm in size, may be subject to misidentification.     

Element concentrations and magnetic susceptibility of anthrosols: Indicators of prehistoric human occupation in the inland Pilbara,Western Australia

The study of archaeological sediments is an important source of information on how humans lived at a site. Attributes of human site use such as frequency of visits and duration of stay can be explored by measuring changes in phosphorus (determined using X-ray fluorescence) and carbon concentrations in sediments and magnetic susceptibility of sediments in combination with analysis of other sediment attributes, stone artefacts and faunal remains. This study concludes that increases in phosphorus and carbon concentrations and the discard rate of stone artefacts at Marillana A rockshelter in the inland Pilbara, Western Australia, indicate an increase in the frequency of site use with no change in the function of the site. This is interpreted as representative of an increase in regional population density.     

The decomposition of starch grains in soils: implications for archaeological residue analyses

Recent research involving starch grains recovered from archaeological contexts has highlighted the need for a review of the mechanisms and consequences of starch degradation specifically relevant to archaeology. This paper presents a review of the plant physiological and soil biochemical literature pertinent to the archaeological investigation of starch grains found as residues on artefacts and in archaeological sediments. Preservative and destructive factors affecting starch survival, including enzymes, clays, metals and soil properties, as well as differential degradation of starches of varying sizes and amylose content, were considered. The synthesis and character of chloroplast-formed ‘transitory’ starch grains, and the differentiation of these from ‘storage’ starches formed in tubers and seeds were also addressed. Findings of the review include the higher susceptibility of small starch grains to biotic degradation, and that protective mechanisms are provided to starch by both soil aggregates and artefact surfaces. These findings suggest that current reasoning which equates higher numbers of starch grains on an artefact than in associated sediments with the use of the artefact for processing starchy plants needs to be reconsidered. It is argued that an increased understanding of starch decomposition processes is necessary to accurately reconstruct both archaeological activities involving starchy plants and environmental change investigated through starch analysis.     

Experiments with Lithic Tools: Understanding Starch Residues from Crop Harvesting

Stone knives were used widely in Neolithic East Asia, presumably in the harvesting of grain crops, but their function has not been clearly understood due to the lack of study of residues from these tools. To address this issue, starch grain analysis was employed to study the residues on the surface of ancient stone knives and large amounts of starches were recovered. The sources of these starches, however, were not well understood, because harvesting of crops involves the cutting of stems rather than direct contact with starchy seeds. To determine whether harvesting could deposit these types of residues, we designed a simulation experiment using stone flakes to harvest ears of wheat, rice and foxtail millet, then analysed the residues on both the flakes and in the plant tissues. A large number of starch grains were found in the stems, including both typical morphotypes from seeds and newly described types that occur only in stems, which can be used as indicators of harvesting. Our study demonstrates that starch grains from residues on the surfaces of archaeological stone knives can indicate not only that the tools were used to harvest ears, but also the type of crops harvested.     

CUT ABOVE THE REST: A MULTI-DISCIPLINARY STUDY OF TWO SLATE KNIVES FROM FORAGER CONTEXTS IN COASTAL NORWAY

Slate was a prominent tool material in the Scandinavian Stone Age. However, details of tool function have relied on morphology and have added little to our understanding of their role in hunting and processing. Here, we demonstrate that it is possible to identify both the use-wear traces and residues from slate knives from northern Norway. By applying a multi-disciplinary approach incorporating experimentation, use-wear and organic residue analyses, we identified residues, including seal hair, and use-traces which indicate the tools were used to process fresh marine mammals.     

Middle Stone Age pièces esquillées from Sibudu Cave,South Africa: an initial micro-residue study

In this paper I present the results of a micro-residue study conducted on ten pièces esquillées (scaled pieces) from Sibudu Cave, South Africa. These artefacts are associated with the Howiesons Poort Industry (∼61.7 and ∼64.7 ka years ago at Sibudu), representing part of the later phase of the Middle Stone Age. Until now, it was unclear on what these pieces were used, and whether they were functional. Previous experimental use-wear work tentatively pointed towards bone processing. However, replication work on stone tool production technology suggests that pièces esquillées are merely the by/end-product of bipolar knapping. I used residue analysis on the Sibudu artefacts because this alternative method has the potential to identify if they were used, and if so, illuminate the specific materials the pieces were used on. Although the sample is small, all the pièces esquillées reveal a clear animal processing signal. There are some bone deposits on the utilised edges that may substantiate bone processing, or perhaps a bone hammer was used with them, but additional study, including Later Stone Age artefacts, is needed to assess the feasibility of these observations. It remains possible that the artefacts are core reduced pieces that were subsequently used as tools or simply knapped with a bone hammer.     

Microwear study of quartzite artefacts: preliminary results from the Middle Pleistocene site of Payre (South-eastern France)

Preliminary functional results obtained from the quartzite assemblage of the Early Middle Palaeolithic site of Payre (South-eastern France) are presented. In an area rich in flint, hominins at Payre also collected quartzite in their local environment, specifically along the Rhône River banks. Although the Payre lithic assemblage is largely composed of flint, quartzite was introduced in the site mainly as large cutting tools knapped outside. This fact pointed out an apparently highly differential treatment of the raw material types available in the region. A major concern is to understand the reason why. Is there any functional reason for the introduction of those artefacts, perhaps to perform specific activities related to the toughness of quartzite? Or is there any functional differentiation among the various raw materials? Use-wear analysis is a useful tool for better understanding human technological choices and strategies of lithic raw material management. Before attempting to extensively apply use-wear analysis on the quartzite assemblage, we analysed a limited sample to evaluate the general surface preservation. A specific experimental programme with the same local quartzite was carried out in order to provide a reliable comparative reference for interpreting use-wear evidence on archaeological implements. Methodological difficulties related to use-wear analysis applied to quartzite artefacts are also discussed. Both Optical light microscopy (OLM) and Scanning Electron Microscopy (SEM) were employed in this study; however, interpretations were elaborated considering principally SEM micro-graphs.The analysis of the archaeological material showed a good state of preservation of the surfaces with a low incidence of post-depositional alterations. The documented use-wear allowed us to identify the active edges, the kinematics and, more rarely, the worked material. Chopping activities were documented on two large artefacts suggesting a specific utility of those tools.     

Necessary for Life: Studies of Ancient and Modern Grinding Stones in Highland Ethiopia

Ethnoarchaeological research combined with morphological analysis of modern and ancient grinding stones was completed in 2013 and 2014 as part of the Eastern Tigrai Archaeological Project (ETAP), based in the Gulo Makeda region of northern Ethiopia. Research focused on investigating the cultural context of grinding, grinding stone morphology, and use-surface area employing low power use-wear analysis of grinding surfaces. In the Gulo Makeda region today, men manufacture grinding stones and women use them in food processing for many hours per day. While grinding, relationships are formed and maintained with other women, and archaeological evidence suggests that women were grinding together in the past. It was discovered through interviews that distinctive grinding surfaces are preferred for processing different cereals based on grain size. Rough surfaces are used to process larger grains such as imported Near Eastern barley (Hordeum vulgare), wheat (Triticum spp.), maize (Zea mays), and the African domesticate sorghum (Sorghum bicolor), while fine/smooth surfaces are used for smaller, locally domesticated African grains including finger millet (Eleusine coracana) and t’ef (Eragrostis tef). Comparing ethnoarchaeological data with evidence of different use-surfaces on ancient Mezber grinding stones suggests that both locally domesticated and imported grains have been processed in the region for thousands of years. Interpretations are made about the use of grinding stones in the past through analogy, supported by evidence of ancient stone tool morphology and surface wear. The archaeological record at the pre-Aksumite site of Mezber indicates that grinding stone sizes have changed through time, with larger grinding surface areas likely reflecting the need for the production of greater amounts of flour.     

Seeing red: the use of Congo Red dye to identify cooked and damaged starch grains in archaeological residues

Starchy plant foods are significant in the diet of almost all peoples. Archaeologically, however, preservation of such plants is limited, and direct evidence of plant use by past people is also rare. Although starch grains can be preserved on artefacts used to process starchy plants, it is very difficult to identify grains damaged by processing methods such as milling or cooking. We present a method for identifying such damaged starch grains using Congo Red staining to identify cooking or milling activities in the past subsistence behaviour of Aboriginal people of southeast Queensland, Australia.     

The changing abundance of backed artefacts in south-eastern Australia: a response to Holocene climate change?

The timing of the introduction, proliferation and decline of backed artefacts in Australia has been much debated. In south-eastern Australia, after initially appearing at least 8500 years ago, backed artefacts greatly increased in number between 4000 and 3500 years ago to the extent that they are found in numerous sites and are recorded in large numbers in individual sites from that time. From about 1500 years ago they declined markedly in number and had seemingly disappeared by the time of British colonization. Models explaining their proliferation advance the proposition that increased backed artefact production was triggered by heightened foraging risk and/or social re-organisation brought about by a change in climate to a regime which was cooler and drier than any other time during the Holocene combined with intensified ENSO climatic conditions. Our study develops this hypothesis by inferring the use of backed artefacts at Mussel Shelter in the Sydney Basin through an integrated use-wear and residue analysis. These inferences provide new insights into the nature of the evolutionary changes in tool production and use in response to the period of altered climatic conditions.     

Late Pleistocene to Early Holocene Plant Movements in Southern Kyushu, Japan

From the late Pleistocene to early Holocene in Japan, subtropical and temperate forest elements moved northwards. This affected human choices and access to food sources. More settled patterns of living spread northwards gradually, and northern hunting-gathering-fishing people began cultivating vegetables and cereal crops. This poster reports the presence of ancient starch residues on stone artefacts in Kagoshima Prefecture, southern Kyushu. The oldest residues recovered are dated by context to about 30,000 ¹⁴C yr BP. If such residues can be identified, it may be possible to detect a hypothesized early phase of tropical plant movement northwards during warmer climate peaks in the late terminal Pleistocene, as well as during the long period of Holocene warming that followed. As an initial step towards identification, the morphological characteristics and condition of the starch granules are described and compared to those of other sites in early Japan.     

Using plant microfossils from dental calculus to recover human diet: a case study from Tell al-Raqā'i,Syria

Dietary reconstructions based on plant microfossils, such as starch grains and phytoliths, have been useful in increasing our understanding of past human populations. Microfossils have been recovered from sediments, stone tools, and, more recently, dental calculus. Methods for recovering microfossils from dental calculus have yet to be firmly established and there is some question about potential damage to the teeth. Using a sample of teeth from the middle Holocene site of Tell al-Raqā'i, Syria, we tested using a dental pick to sample the calculus. ESEM images taken before and after sampling show no damage to the enamel surface, and examination of the recovered microfossils show that this method provides ample material for study, even when not all of the calculus is removed from the tooth. Preliminary identification of the plant microfossils suggests that these individuals were consuming a variety of plant foods, but that domesticated cereals such as wheat and barley made up a surprisingly small portion of their diet.     

Changes in starch grain morphologies from cooking

One of the three main plant microfossils, starch grains are increasingly used as markers of diet, plant domestication, tool use and site organization, because their morphology and features provide a means to identify the plant that produced them. However, starch grains are susceptible to damage when they are exposed to heat in the presence of water, as in cooking. We documented the changes that occur in the starch grains of 10 domesticated plant species due to exposure to different cooking methods, in order to better understand how cooking alters the appearance of the grains, and if these cooking methods might be identifiable in the archaeological record. Our results show that some cooking methods produce unique, identifiable damage on some types of plant starches, but generally each plant species reacts uniquely to cooking. In order to record the changes for each plant species, we have created a database, available at (http://www.osresearch.net/∼hollyf/starchdb/index.cgi), to which registered users can add their own images of cooked starch grains.     

A Multi-Method Approach to Inferring Early Agriculturalists’ Stone Tool Use at the Crescent Bay Hunt Club Site

While there has been much research on the function of stone tools via use-wear analysis, it is clear that a multipronged approach, including an evaluation of material acquisition, production, and tool use, is necessary if tool function is going to prove truly useful for understanding past cultures. Moreover, the role of chipped lithic tools in the economies of agriculture-based populations has seen little research compared to preagricultural systems. A sample of lithic artifacts from the Crescent Bay Hunt Club site, a twelfth- to fourteenth-century Oneota village at Lake Koshkonong in southeastern Wisconsin, are subjected to a multiple-method analysis to determine individual tool use. An assemblage-based analysis provides an overall understanding of the lithic economy. A combination of microscopic identification of edge damage and surface polishes and an analysis of protein residue provides independent lines of evidence that yield strong inferences about tool use in the lithic economies of sedentary agricultural groups in the midcontinent.     

Sickle blade life-history and the transition to agriculture: an early Neolithic case study from Southwest Asia

In Southwest Asia, sickle blades first appear early in the sequence of the transition to agriculture. In the past, detailed qualitative research on silica bearing blade stone tools focus on the characterization of use-wear traces such as polish types and accrual rates. In this paper we approach the study of sickle blades slightly different, choosing to examine tool life-history by developing a method to quantitatively estimate harvesting intensity. The method centers on an experiment of cutting cereal stalks and measuring stone blade edge thickness under a scanning electron microscope as a proxy for cutting time. We end with regressing the experimental results to provide an estimation of how intensively archaeological sickle blades recovered from the site of Dhra’, Jordan were used for harvesting. The results, while preliminary, enable an initial interpretation of sickle blades as important tools with long use-life histories during the early Neolithic in the Southern Levant.