Calcium isotopes in archaeological bones and their relationship to dairy consumption

The calcium isotope ratios (δ^44/42Ca) of bones from humans and fauna from three archaeological sites, Taforalt, Abu Hureyra, and Danebury, are evaluated in order to assess whether calcium isotope ratios of bones can be used to detect dairy consumption by adult humans. At each site the fauna δ^44/42Ca is the same regardless of species, while the humans have lower δ^44/42Ca than the local animals by 0.24–0.41‰ (site means). However we cannot ascribe this difference to dairy consumption, given this human–faunal difference also occurs in Epipalaeolithic and Mesolithic adult humans, where dairy consumption is unlikely. Rather, this difference appears to be a result of differences in metabolic processes or other aspects of diet between humans and fauna. Minimal isotopic change in sequential acid leaches of bone powders and consideration of the high calcium concentration in bone suggest that bone calcium isotope ratios are not substantially affected by diagenetic change.     

The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices

Recent archaeological studies of human diet have used stable nitrogen isotope ratios (δ¹⁵N) from human bone collagen to infer the relative importance of terrestrial plant and animal foods. This approach is based on widely observed enrichment of δ¹⁵N up the food chain, plants having distinctly lower values than the herbivores that consume them. Studies of early farming diets in Britain, Denmark and Germany have tended to detect relatively high δ¹⁵N values (e.g. c. +9‰), interpreted as evidence of a diet largely based on animal products, though archaeobotanical evidence for crop cultivation (e.g. carbonised cereal grain and chaff) is widespread. This paper investigates the impact of manuring on δ¹⁵N values in modern cereals, and of charring on these cereal values. The results from two long-term experiments demonstrate that manuring significantly raises δ¹⁵N in cereal grain and chaff. Depending on manuring levels and frequency, it appears that human diets with a major component of such grain would conventionally be interpreted as indicating a largely animal-based diet or a mixed plant/animal diet. Moreover, preliminary analyses of experimentally charred grain and chaff from manured and unmanured conditions are promising for the extraction of reliable ancient δ¹⁵N values from archaeobotanical cereal remains. The wider implications of these results, and the need for further work, are discussed.     

Stable isotope analysis of human and animal remains from the Late Upper Palaeolithic site of Balma Guilanyà, southeastern Pre-Pyrenees,Spain

Stable isotope analysis of carbon (¹³C/¹²C) and nitrogen (¹⁵N/¹⁴N) was performed on collagen extracted from three human and five herbivore bone and tooth samples from the Late Upper Palaeolithic site of Balma Guilanyà (Catalonian Pre-Pyrenees, Spain). Contextual and palaeoecological data as well as radiocarbon dates indicate that the studied occupation phase took placed during the Bolling/Allerod interstadial (GI-1a event). The human remains were co-mingled without any anatomical association, corresponding to a minimum number of three individuals, and it was not possible to determine if the three analyzed samples are from one or more individuals. The mean isotope values obtained from the human remains are δ¹³C = −19.8‰ and δ¹⁵N = 6.7‰, while those of the large herbivores (red deer and wild goat) were −19.8‰ and 1.7‰ for δ¹³C and δ¹⁵N respectively. This indicates that the main source of protein in the diet of the Balma Guilanyà human(s) came from terrestrial herbivores. There is no zooarchaeological or isotopic evidence for the consumption of freshwater or marine resources at the site, which lies 80 km from the present Mediterranean coast. The low δ¹⁵N values observed in both human and animal samples correspond to a trend reported by other researchers working in northwestern Europe: a significant δ¹⁵N reduction in collagen from bones datable within 20,000–10,000 BP, followed by a rise to present values in the Early Holocene. This phenomenon, generally attributed to climatic and/or pedological processes, had not been previously observed in the Mediterranean region and, until now, was thought to be restricted to northern Europe.     

Chemical analyses of organic residues in archaeological pottery from Arbon Bleiche 3, Switzerland – evidence for dairying in the late Neolithic

Fatty acids distribution and stable isotope ratios (bulk δ¹³C, δ¹⁵N and δ¹³C of individual fatty acids) of organic residues from 30 potsherds have been used to get further insights into the diet at the Late Neolithic (3384–3370 BC) site of Arbon Bleiche 3, Switzerland. The results are compared with modern equivalents of animal and vegetable fats, which may have been consumed in a mixed ecology community having agrarian, breeding, shepherd, gathering, hunting, and fishing activities. The used combined chemical and isotopic approach provides valuable information to complement archaeological indirect evidence about the dietary trends obtained from the analysis of faunal and plant remains. The small variations of the δ¹³C and δ¹⁵N values within the range expected for degraded animal and plant tissues, is consistent with the archaeological evidence of animals, whose subsistence was mainly based on C₃ plants. The overall fatty acid composition and the stable carbon isotopic compositions of palmitic, stearic and oleic acids of the organic residues indicate that the studied Arbon Bleiche 3 sherds contain fat residues of plant and animal origin, most likely ruminant (bovine and ovine). In several vessels the presence of milk residues provides direct evidence for dairying during the late Neolithic in central Europe.     

Stable isotopes and diet at Ancient Kerma,Upper Nubia (Sudan)

Carbon and nitrogen stable isotope values were measured from bone collagen extracted from archaeological Nubian human (n = 54) and faunal (n = 61) populations from the site of Kerma, Sudan. Collagen suitable for isotopic analysis was extracted from 22 faunal and 48 human samples from the Eastern cemetery site, dated to the Middle Kerma (c. 2050–1750 BC) and Classic Kerma (c. 1750–1500 BC) periods respectively. The isotopic data indicate that the human dietary regimen included a mix of C₃ and C₄ plant-derived components, with a larger C₄ component than previously reported in archaeological Egyptian Nile Valley populations. Elevated δ¹⁵N values are attributed to consumption of dietary resources from a ¹⁵N-enriched terrestrial ecosystem. The faunal isotope data also indicate the consumption of C₃ and C₄ plants. The large range of δ¹³C values measured in both the human and faunal samples supports previous work suggesting that a significant portion of the populations buried at Kerma may have originated elsewhere, further confirming the Nile Valley as a major corridor for population movement in the region.     

Microstructural,chemical and isotopic evidence for the origin of late neolithic leather recovered from an ice field in the Swiss Alps

Archaeological leather samples recovered from the ice field at the Schnidejoch Pass (altitude 2756 m amsl) in the western Swiss Alps were studied using optical, chemical molecular and isotopic (δ¹³C and δ¹⁵N of the bulk leather, and compound-specific δ¹³C analyses of the organic-solvent extracted fatty acids) methods to obtain insight into the origin of the leather and ancient tanning procedures. For comparison, leathers from modern native animals in alpine environment (red deer, goat, sheep, chamois, and calf/cow) were analyzed using the same approach. Optical and electron microscopically comparisons of Schnidejoch and modern leathers showed that the gross structure (pattern of collagen fibrils and intra-fibrils material) of archaeological leather had survived essentially intact for five millennia. The SEM studies of the hairs from the most important archaeological find, a Neolithic leather legging, show a wave structure of the hair cuticle, which is a diagnostic feature for goatskins. The variations of the bulk δ¹³C and δ¹⁵N values, and δ¹³C values of the main fatty acids are within the range expected for pre-industrial temperate C₃ environment. The archaeological leather samples contain a mixture of indigenous (from the animal) and exogenous plant/animal lipids. An important amount of waxy n-alkanes, n-alkan-1-ols and phytosterols (β-sitosterol, sitostanol) in all samples, and abundant biomarker of conifers (nonacosan-10-ol) in the legging leathers clearly indicate that the Neolithic people were active in a subalpine coniferous forest, and that they used an aqueous extract of diverse plant material for tanning leather.     

Trace element concentrations in teeth – a modern Idaho baseline with implications for archeometry,forensics, and palaeontology

Concentrations of 69 minor and trace elements were measured in situ using laser ablation inductively-coupled plasma mass spectrometry in multiple teeth of modern herbivores (Cervus elaphus, Odocoileus hemionus, Odocoileus virginianus; n = 52), omnivores (Ursus americanus; n = 56), and carnivores (Felis concolor; n = 33; Canis lupus; n = 4) from Idaho, USA. On each tooth, two to five analyses were collected on enamel, primary dentine, and secondary dentine with spot diameters of 30–100 μm. Continuous 30-μm-wide line traverses were collected across deer, elk, and wolf teeth. Results confirm trace element concentrations below 1 ppm for many elements, including Li, Be, transition metals (excepting Mn, Fe and Zn), heavy metals (excepting Hg and Pb), the rare earths, and actinides. Strontium and Ba generally biopurify with increasing trophic level, but Ba shows decreasing discrimination with tissue type in the order secondary dentine – primary dentine – enamel. Most elements show strong zoning within tissues and variable trophic level behavior, so concentrations in omnivores rarely fall between herbivores and carnivores. Trace element mass balance suggests that soil and dust, not food, commonly deliver the largest trace element load in terrestrial carnivores and omnivores, even for elements that biopurify, like Ba. Use of trace elements for inferring past diets are likely compromised by unknown rates of dust and soil ingestion rather than indicating the degree of herbivory and carnivory. The low concentrations of many elements provide multiple sensitive markers of post-burial overprinting that can be used to discriminate altered vs. original biogenic compositions. REE and actinide trends do not directly follow bedrock compositions.     

Introducing δSr analysis in archaeology: a demonstration of the utility of strontium isotope fractionation in paleodietary studies

Isotopic methods are widely used in archaeology to investigate paleodiet. Here, we present a new method to identify trophic level in archaeological human populations and to investigate paleodiet. We demonstrate that strontium isotope compositions (reported as δ^88/86Sr) vary in a mass-dependent manner with increasing trophic level and can elucidate paleodiet in archaeological human populations. We present new mass-dependent strontium isotope data from tooth enamel and bone from individuals buried during the Late Intermediate Period (c. AD 1000–1300) in the large cemeteries of Chiribaya Alta, Chiribaya Baja, San Gerónimo, and El Yaral in the Ilo and Moquegua Valleys of southern Peru. We compare these data to radiogenic strontium isotope data (⁸⁷Sr/⁸⁶Sr) and light stable isotope data (δ¹⁵N_col and δ¹³C_col) from the same individuals to investigate geologic variability in strontium sources as well as marine food consumption among the Chiribaya. Our results demonstrate the utility of measurements of strontium isotope fractionation as a new tool for archaeological investigation of paleodiet. Importantly, this new technique can be used to generate paleodietary (δ^88/86Sr) and paleomobility (⁸⁷Sr/⁸⁶Sr) data from the same specimen, minimizing destructive analyses of invaluable archaeological material, and provides a new way to examine paleodiet through hydroxyapatite, which is particularly important when collagen is poorly preserved.     

Dietary change in Fijian prehistory: isotopic analyses of human and animal skeletal material

We examine evidence for prehistoric diet in the Fiji Islands through the analysis of stable isotope ratios (δ¹³C and δ¹⁵N) for 26 samples of human and animal bones from various archaeological sites, time periods, and local environments. The oldest individuals in this study, dating to about 2700 BP and living on the small island of Waya, consumed a predominantly marine-based diet. Subsequent populations on this island showed reduced consumption of marine resources, with greater reliance on terrestrial ones, throughout the cultural sequence. In contrast, populations of humans and pigs living inland on Viti Levu, the largest island, relied on terrestrial resources since at least 1500 BP. Thus, our results suggest that human and pig diets throughout Fijian prehistory relied variably on marine or terrestrial resources, and this distinction is largely a product of geography. This finding and our analyses provide a model for understanding ancient diets in Remote Oceania.     

Isotopic investigation of pre-Hispanic macaw breeding in Northwest Mexico

The pre-Hispanic settlement of Paquimé (ca. 1200–1450 AD) lay outside of urban Mesoamerica in arid northern Chihuahua, Mexico, yet the excavated remains of 322 scarlet macaws, tropical parrots whose northernmost habitat is 500 km to the south, suggest that Paquimé had relationships with distant communities. Here, carbon and oxygen stable isotope analysis of 30 macaw bones determines whether the Paquimeños actively bred these parrots or traded with Mesoamerican groups to obtain the birds. The δ¹³C values indicate that the macaws ate a diet of C₄ plants, foods that were unavailable to wild macaws. In addition, δ¹⁸O data suggest that the majority of the macaws spent their entire lives at Paquimé. We conclude that the ancient Paquimeños were in fact breeding scarlet macaws, although one bone with δ¹⁸O values outside the local range suggests that the breeders also maintained exchange ties with the source areas of the birds. By confirming that Paquimeños implemented a system of local macaw reproduction outside the Mesoamerican region itself, this analysis provides new evidence about the economic and ideological interdependence of pre-Hispanic societies in the Americas. It is also suggestive of the pre-industrial capabilities of humans to extend the natural ranges of animal species.     

Stable carbon and nitrogen isotope analysis on human remains from the Early Mesolithic site of La Vergne (Charente-Maritime,France)

We report here the results of stable carbon and nitrogen isotope analysis of human and faunal remains from La Vergne (Charente-Maritime, western France), a rare Early Mesolithic burial site (ca. 8500–8000 cal BC). The results for nine humans (average δ¹³C = −19.3‰; δ¹⁵N = 9.4‰) indicate a strongly terrestrial diet, dominated by animal protein, with the possibility of, at best, a slight contribution of marine-derived protein. Given lower sea-levels in the early Holocene, the site would have been some 60–80 km from the sea at the time of its use; nevertheless, contacts with the coast are shown by the presence of numerous marine shell beads in the graves. In the light of the stable isotope results, it is suggested here that such contacts most likely took the form of exchange with coastal communities whose remains now lie underwater.     

Oxygen isotopic composition of limpet shells from the Beagle Channel: implications for seasonal studies in shell middens of Tierra del Fuego

Shell middens distributed along the coastal areas of the Beagle Channel (Tierra del Fuego) testify the primary role of marine resources in the successful social dynamic of hunter–gatherer–fisher societies (HGF) in extreme high latitude environmental conditions. Intertidal and subtidal limpet species are frequently identified taxa of these archaeological deposits. We investigated whether shell δ¹⁸O of the limpet species Nacella deaurata is a valuable record of local sea surface temperature (SST) and ambient water δ¹⁸O (δ¹⁸Ow) fluctuations, and in turn can be used to estimate the season of its exploitation by Holocene HGF. Sequential shell δ¹⁸O of modern species track seasonal changes of SST and δ¹⁸Ow. The comparison of shell δ¹⁸O profiles of N. deaurata and Nacella magellanica (a coexisting species) suggests the occurrence of distinct growth rate and physiological adaptations between species. Shell δ¹⁸O of zooarchaeological specimens of N. deaurata from Lanashuaia II suggests that animals were exploited predominantly in winter and that experienced similar environmental conditions of present day at ∼1320 years BP. Results also indicate that N. deaurata is a valuable candidate for studying past seasonal changes in SST and δ¹⁸Ow in this southernmost part of South America.     

Human–environment interactions in medieval Poland: a perspective from the analysis of faunal stable isotope ratios

Stable isotope analyses of faunal remains provide valuable information about human–environment interactions in the past, including insights into past animal husbandry and land management strategies. Here, we report stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope values of collagen and carbonate from archaeological fauna from Ka?dus, a medieval settlement in North-Central Poland, to better understand human–environment interactions during a period of increasing urbanism and marketization. Wild and domestic animals can be separated on the basis of their isotopic values. The mean δ¹⁵N value for 12 domesticated animals is 7.6 ± 1.2‰ and for 5 wild animals is 4.3 ± 0.5‰ (p = 0.002). The mean collagen δ¹³C value for domesticated animals is −20.6 ± 1.1‰ and for wild animals is −22.0 ± 0.5‰ (p = 0.004). The mean carbonate δ¹³C value for domesticated animals is −13.14 ± 1.3‰ and for wild animals is −14.14 ± 0.9‰ (p = 0.034). The “canopy effect” and anthropogenic effects that alter stable isotope ratios of plants (manuring, swidden agriculture and ploughing) are discussed in relation to these differences. Fish are isotopically variable, which suggests broad-spectrum fishing strategies and/or trade, and increases our awareness of the difficulties in interpreting human paleodiet when freshwater fish were on the menu.     

Reassessing the northern limit of maize consumption in North America: stable isotope,plant microfossil,and trace element content of carbonized food residue

In combination, the analysis of carbonized food residue for stable C and N isotopes, elemental composition, and plant microfossils (phytoliths and starch granules) offers a powerful tool for understanding patterns of prehistoric maize (Zea mays) consumption in small-scale societies. Using this approach, we conclude that maize was more widely consumed in North America than previously suspected. Between ∼AD 700 and 1600, despite little or no archaeological evidence of gardening, corn was a widespread component of diet on the eastern Canadian prairies. This pattern, furthermore, extended into the adjacent boreal forest at about the same time. However, carbon (δ¹³C) and nitrogen (δ¹⁵N) values on food residue samples vary widely, suggesting significant overall dietary differences from one region to the next. Analysis of a subset of residue samples for elemental composition (using ICP-OES [inductively coupled plasma–optical emission spectrometry]) may help identify broad trends in the provenience of foods consumed at these sites.     

Variation in collagen stable nitrogen values in black-tailed jackrabbits (Lepus californicus) in relation to small-scale differences in climate,soil, and topography

Longstanding observations about the relationship between increasing aridity and ¹⁵N enrichment in mammalian collagen values have led to an interest in their use as a paleoclimatic marker. Here we report on variability in collagen nitrogen values from five modern and two archaeological samples of black-tailed jackrabbits (Lepus californicus) from the eastern Great Basin of the United States (N = 178 individuals). Nitrogen δ¹⁵N values were highly variable within all samples. Modern samples showed significant differences despite similarities in average annual precipitation and temperature. Archaeological samples were indistinguishable from each other or from modern samples taken from the same area despite independent evidence for differences in precipitation for the two prehistoric periods considered. Differences between modern samples were most strongly associated with soil characteristics. We discuss these results in light of their relationship with topography and vegetation and highlight their implications for archaeological applications of stable nitrogen analyses in several contexts.     

Subsistence and the isotopic signature of herding in the Bronze Age Hexi Corridor,NW Gansu,China

Dietary patterns at two Bronze Age sites in the Hexi Corridor are investigated by the analysis of stable carbon and nitrogen isotopes in faunal bone collagen. The findings are compared with archaeobotanical remains from one of the sites which include high proportions of millet (Panicum miliaceum and Setaria italica) as well as the western derived cereals wheat (Triticum aestivum), barley (Hordeum vulgare) and oat (Avena sativa). The isotopic data indicate domestic omnivores (Canis and Sus) had diets dominated by millet. Minimally offset δ¹⁵N values between herbivore and omnivore fauna suggest low consumption of animal protein by omnivores. Diets of herded animal (Bos and Caprinae) included only low proportions of C4 foods, suggesting that these animals were not regularly foddered with millet plants, and that their grazing areas were mostly beyond the agricultural zone. The wide range in δ¹⁵N values amongst herbivore fauna (4.1‰–11.8‰) suggests grazing occurred in a variety of ecological zones, and this would be consistent with the occurrence of long-distance transport of livestock in the region.     

Investigation of palaeodiet in the North Caucasus (South Russia) Bronze Age using stable isotope analysis and AMS dating of human and animal bones

This paper addresses the question of human palaeodietary adaptation in the Eneolithic and Early Bronze Age periods of the North Caucasus (South Russia) using stable isotope analysis. One of the key questions is the presence of fish in the diet. AMS radiocarbon dating of archaeological bone collagen has also been carried out to investigate potential radiocarbon reservoir effects in human skeletal material as a result of an aquatic diet. A fish component has previously been suggested in the diet of Iron Age and Bronze Age humans across Eurasia by isotopic research and radiocarbon dating of contemporaneous human and animal bones. In the North Caucasus however, isotopic data is scant. This study presents a new set of stable isotopic data from several Early Bronze Age sites, mainly belonging to the Maikop culture of the North Caucasus. The results show that the diversity in climate and environment across the northern Caucasus may be a causal factor for the patterns observed in the stable isotope values of terrestrial herbivores. This affects the isotopic values of the humans consuming them. The differences in δ¹⁵N and δ¹³C ranges of both humans and fauna were found to correlate strongly with geography and climate; the most enriched isotopic values are found in the dry steppe areas to the north. Overall, a relatively high enrichment in δ¹⁵N values of humans compared to local terrestrial herbivores and carnivores was observed. This indicates that aquatic resources were probably part of the Bronze Age diet in the region although the extent of this needs further investigation. The dramatic effect of environmental factors on isotopic values in the Early Bronze Age of the North Caucasus illustrates how confident conclusions cannot be drawn on the basis of a small number of samples from widely differing regions and time periods. Radiocarbon dating can provide a useful tool for identifying dietary derived reservoir ages in humans, potentially caused by a fish diet. With two possible exceptions, the nine human–animal bone pair dated as part of this study showed no consistent indication for a consistent reservoir effect.     

Spatial variation of biologically available strontium isotopes (Sr/Sr) in an archipelagic setting: a case study from the Caribbean

This paper presents the results of strontium (Sr) isotope analysis of modern and archaeological animal remains and modern plant samples from the Caribbean region. The goal is to assess if patterns of human mobility can be determined from the archaeological record in an archipelagic setting. The range of variability and spatial distribution of biologically available strontium isotope signatures is reported and data evaluated to determine if sufficient heterogeneity exists to permit the identification of mobility despite the presence of potentially large contributions of marine strontium in island and coastal ecosystems. The (is)landscape is divided into several sub-regions based on the age and lithology of underlying geology and the variability of ⁸⁷Sr/⁸⁶Sr ratios is reported as; mean ⁸⁷Sr/⁸⁶Sr ± 2 standard deviations, number of samples: 1) Volcanic and Intrusive rocks (0.7077 ± 0.0019; n = 162); 2) Cretaceous–Miocene Limestone (0.7085 ± 0.0009; n = 50); 3) Pliocene–Quaternary Limestone (0.7091 ± 0.0004; n = 54); 4) Sedimentary Deposits (0.7094 ± 0.0015; n = 16); 5) Metamorphic Deposits (0.7104 ± 0.0014; n = 6). There are substantial differences between expected ⁸⁷Sr/⁸⁶Sr values based on associated geology and measured ⁸⁷Sr/⁸⁶Sr in flora and fauna samples. These differences emphasize the importance of focusing on bioavailable strontium, as opposed to geological proxies, for estimations of Sr isotope ranges, especially in archipelagic environments. While some overlap exists between the ⁸⁷Sr/⁸⁶Sr ranges of certain sub-regions, other sub-regions possessed limited variation and could be distinguished based on strontium isotope data. This spatial patterning of biologically available strontium indicates that strontium isotope analyses can be successfully applied to the archaeological study of human mobility within the Caribbean region.     

Funeral practices and foodstuff behaviour: What does eat meat mean? Stable isotope analysis of Middle Neolithic populations in the Languedoc region (France)

The aim of this study is to reconstruct the dietary patterns and economic behaviours of Neolithic populations in the Northwestern Mediterranean using isotopic and archaeological data. Burials come from four sites located in Languedoc–Roussillon region in French Mediterranean area. These sites are dated from the Middle Neolithic period (ca. 4500–3500 BC). They represent the Chasséen culture, characterized by regional features, such as economy management, resulting from territorial control. For this investigation, a stable isotopes (δ¹³C and δ¹⁵N) method has been used on 50 human bone collagens and 28 associated animal bones. This method provides direct dietary information on the protein consumed including the relative amounts of marine vs. terrestrial and animal vs. plant proteins in diets. Isotopic results are mainly compared to archaeological data to understand economic distinctions and potential social status variations between different groups using specific funeral practices, i.e. lithic chamber graves vs. domestic/funeral pits. Results show that individuals buried in lithic chamber graves and those buried in pits did not have the same dietary pattern. This result suggests a possible differentiation between two socio-economic groups, i.e. consumers of resources from herding and from farming. No aquatic food appeared to be routinely consumed by these individuals despite a relative close proximity to sea and freshwater sources. Moreover, these outcomes lead us to hypothesize that: (1) funeral practices could be linked to specific economies and/or (2) to different social status and that (3) burial type and foodstuff could be an expression of religious worship. Further research could include data from other areas, such as Spanish Catalonia where there are funeral structures similar to Languedoc lithic chamber graves.     

Palaeolimnological impacts of early prehistoric farming at Lough Dargan,County Sligo,Ireland

This study provides a unique method of inquiry for archaeological investigation with an aim to assess the intensity and effects of Neolithic and Bronze Age farming practices at Lough Dargan, northwest Ireland, through a multi-proxy analysis of a lake sediment core. Chironomid (non-biting midge fly) subfossils and lake sediment geochemistry (δ¹³C, δ¹⁵N and C:N ratios) were used to assess changes in limnological conditions through time. The limnological data were compared with macroscopic charcoal concentration and pollen data to examine the potential influence that early farmers had on a freshwater lake system within a prehistorically active catchment. Results from the chironomid analysis show that the first substantial period of agricultural activity in the early Neolithic (c. 3730–3190 BC) resulted in a temporary shift to more eutrophic lake conditions. There is evidence of animal husbandry with substantial levels of animal waste reaching the lake, leaving an imprint in the geochemical record of increased δ¹⁵N values and decreased δ¹³C values and C:N ratios during this time. The chironomid community reverted back to its pre-impacted state c. 3190 BC in response to a period of reduced farming (c. 3390–3000 BC) which eventually led to a distinct lull in activity, with possible cessation of farming from 3000 to 2700 BC. A return to eutrophic conditions coincided with the gradual return of agriculture, with more permanently altered lake conditions dominating from 2400 BC, even during a 250-year period of reduced human activity commencing at c. 1440 BC. Increased sedimentation rate, along with increases in δ¹³C, δ¹⁵N and C:N, the presence of chironomid taxa indicative of erosion, more eutrophic lake conditions and high concentrations of macroscopic charcoal all point to more intensive land use practices during the Bronze Age. Palaeolimnological data exhibited an immediate response to intensified farming during this time, and were especially responsive to pastoral farming due either to scale of activity or proximity to the lakeside. The success of this study demonstrates the effectiveness of palaeolimnological analysis in the investigation of prehistoric farming. This approach will help inform Neolithic and Bronze Age land-use practice and human–environment relations in the region, and highlights the potential for chironomid-based archaeological research.