Diet,social differentiation and cultural change in Roman Britain: new isotopic evidence from Gloucestershire

This study uses stable isotope analyses (δ¹³C and δ¹⁵N) of human bone collagen to reconstruct the diet of three Romano-British (first to early fifth century AD) populations from Gloucestershire in South West England. Gloucestershire was an important part of Roman Britain with two major administrative centres at Gloucester (Glevum) and Cirencester (Corinium) and numerous smaller settlements and farmsteads. To investigate potential dietary differences between the rural and urban populations of Roman Gloucestershire, we compared human bone collagen stable isotope values from 32 individuals from urban Gloucester with those of 46 individuals from two rural cemeteries at Horcott Quarry and Cotswold Community, respectively. Seven individuals from urban Gloucester were buried in a mass grave; all others were buried in single inhumations. Results show small but significant differences in stable isotope ratios between the urban and rural populations which indicate that the urban population might have consumed slightly more marine and/or freshwater resources than the people living in the rural communities. We interpret this difference as a direct reflection of Rome’s influence on Gloucester’s population and the town’s economic status. Subtle differences in stable isotope ratios were also observed at the site level, as burial practice does correlate with diet in some cases. Overall, the results from this study demonstrate that diet, as reconstructed through stable isotope analysis, is a very sensitive, if settlement-specific, indicator of social differentiation and culture change.     

The effect of parity status on δN: looking for the “pregnancy effect” in 18th and 19th century London

This study examined the effect of parity status on δ¹⁵N using the well-characterised 18th and 19th century skeletal collection from the crypt of Christ Church, Spitalfields, London. We tested whether the cumulative effect of multiple pregnancies and breastfeeding could significantly reduce female δ¹⁵N values compared to males. The results from stable isotope analysis of 92 adult ribs show that the population of Spitalfields had relatively little variation in diet, compared to contemporary urban populations, and had abundant animal and marine protein. We were able to rule out any effect attributable to socio-economic status, date or age at death on the stable isotope ratios. There were no significant differences in δ¹⁵N due to parity status, nor were there any differences between males and females. Models of collagen turnover rates in ribs suggest that the effect of δ¹⁵N depletion due to pregnancy would be undetectable except in ideal circumstances, where bone of the optimal turnover period was sampled, and in cases where multi-parous individuals died shortly after parturition.     

Carbon and nitrogen stable isotope analysis on the diet of Jomon populations from two coastal regions of Japan

We report on a stable isotope paleodietary reconstruction of Jomon populations in Japan during the Middle to Final Jomon period (ca. 5000–2300 years BP), focusing on dietary differences within and among populations and between regions. Carbon and nitrogen isotope analysis was performed on human and faunal bone collagen from six coastal sites along the Inland Sea in the Sanyo (Ota, Funamoto, and Tsukumo) region and along Mikawa Bay and the Pacific Ocean in the Tokai (Kawaji, Yoshigo, and Inariyama) region. We found that carbon and nitrogen isotope ratios were positively correlated, indicating that the Jomon people consumed a mixed diet of marine (shellfish and marine fish) and terrestrial (C₃ plants and terrestrial mammals) protein. In the Ota samples (n = 25, during the Middle Jomon period, 5000–4000 years BP), sex was one of the main reasons for the intra-population dietary variation. Ota males consumed greater amounts of marine food, while Ota females consumed greater amounts of terrestrial food; these dissimilar diets may have been related to the sexual division of labor. Significant inter-population dietary differences were found, which may have been related to differences in age or site location. Notably, the two coastal regions showed clear isotopic differences. Nitrogen isotope ratios of individuals from the Sanyo region were significantly higher than ratios of individuals from the Tokai region. The individuals in the Sanyo region might have consumed a diet high in aquatic foods, particularly high trophic level marine fish, whereas the individuals in the Tokai region might have consumed a lot of marine shellfish. Another possible reason for the regional isotopic difference might have been different baseline of nitrogen isotope ratios of the marine ecosystems.     

CARBON ISOTOPE RATIOS IN PALAEODIET: LACK OF AGE OR SEX EFFECT

Palaeodiet of humans can be determined using stable carbon isotope ratios of bone collagen. Differences in δ¹³C-values between individuals in a population may be due to real differences in diet, or alternatively, they could be due to age- or sex-dependent differences in the physiologically controlled fractionation of carbon isotopes between food and collagen. The dependence of this fractionation on age and sex was tested in a study of 50 individuals of differing ages from a prehistoric population of bison hunters. The total variation in δ¹³C for bone collagen was found to be ± 0.3‰, indicating that variations larger than this, observed in other populations, are due to real dietary differences between individuals.     

Immigration to Tikal,Guatemala: Evidence from stable strontium and oxygen isotopes

This paper presents strontium and oxygen isotopic measurements on archaeological human teeth from the ancient Maya city of Tikal, Guatemala, that illuminate the role that migration played in the history of the state. Stable strontium isotope ratios of human teeth parallel the bedrock geology of the location where foods were grown, while stable oxygen isotope ratios reflect the sources of water imbibed, and track geographic variation in the isotopic composition of rain water. Because tooth enamel forms during childhood and is not remodeled during life, we can identify foreign-born individuals at Tikal by their outlying strontium and oxygen isotope ratios. These data indicate that approximately 11–16% of the sampled Tikal skeletons spent their childhood at distant sites. Most of the migrant burials date from the Early Classic period and are high status contexts. Several royal burials demonstrate long distance movement of both males and females, and shed light on the identification of epigraphically-known individuals. Yet, both Early and Late Classic migrants are found in lower status domestic burials. Interaction with distant peers was important in the rise of the Tikal polity, however, immigration from all social tiers contributed to the city’s rapid population growth.     

Strontium and stable isotope evidence for diet and mobility in Roman Gloucester,UK

This paper presents the results of a multi-isotopic (oxygen, strontium, carbon and nitrogen isotopes) investigation of population and dietary diversity in Roman Gloucester, focusing on individuals found in a late 2nd century AD mass burial pit at London Road, and comparing them to those found in the nearby cemetery. There were no statistical differences in isotopic composition between mass grave and cemetery burials, suggesting, in agreement with the osteological evidence, that the mass burial was the result of a catastrophic event, probably an epidemic disease. Strontium and oxygen isotope analysis demonstrated considerable diversity in the origins of the Gloucester population, with evidence for both UK and non-UK individuals. Diet was predominately terrestrial and similar to that of other Romano-British populations. Elevated δ¹³C_dentine ratios in some individuals are correlated with raised δ¹⁸O_p values and are therefore probably due to childhoods spent in warmer climates, rather than dietary variation.     

Exploring subsistence and cultural complexes on the south coast of Papua New Guinea using palaeodietary analyses

We present the results of a palaeodietary study of a skeletal sample (～800–300 BP) from the south coast of Papua New Guinea (Nebira, site ACJ) using multiple stable isotope analysis of bone collagen. The carbon, nitrogen and sulphur stable isotope ratios of 28 individuals (n = 12 males, n = 13 females and n = 3 subadults) suggested the diet at Nebira was based on C₃ plants (likely starchy vegetable staples) and included protein resources from the surrounding forested areas and C₃/C₄ savannah grasslands such as wallaby and other wild animals. Domestic species (e.g. Sus scrofa, Canis familiaris and Gallus gallus) may also have been consumed but could not be differentiated from wild species by stable isotope analysis. There were no significant differences in stable isotope values between males and females, but the δ³⁴S values of the juveniles suggest they may have consumed varied protein resources. The sulphur stable isotope ratios indicate there was no discernable marine component in the diet of any of the individuals from Nebira. The stable isotope results are interpreted within a wider context of Papuan south coast trade and exchange systems in an attempt to understand local interaction in the region.     

The children of Kaminaljuyu: Isotopic insight into diet and long distance interaction in Mesoamerica

Known for its spectacular tombs and adobe talud–tablero architecture, the highland Guatemalan city of Kaminaljuyu is key to models of long distance interaction in Mesoamerica. We use stable isotopic data from human bone, dentine and tooth enamel to reconstruct Kaminaljuyu’s dietary history. Stable carbon isotope ratios and alkaline earth ratios of enamel carbonate indicate a decline in maize consumption from Preclassic to Classic periods, perhaps due to the desiccation of Lake Miraflores, which was used to irrigate Late Preclassic fields. Stable oxygen and strontium isotope ratios in enamel shed light on the geographic origin of Early Classic skeletons, and show that the central skeletons in the tombs were local children. However, four decapitated skulls and two peripheral skeletons show enriched oxygen ratios, similar to Lowland Maya sites. Strontium isotope ratios indicate that most of these are from an area underlain by Cretaceous limestones; one is from a metamorphic region. Two individuals may have traveled to or from Central Mexico. The greater evidence for lowland individuals among the tomb skeletons implies that political connections with the Maya area were more significant to elites at Kaminaljuyu than was direct contact with Central Mexico.     

‘THERE'S NO PLACE LIKE HOME’—NO ISOTOPIC EVIDENCE FOR MOBILITY AT THE EARLY BRONZE AGE CEMETERY OF SINGEN,GERMANY

The Early Bronze Age necropolis of Singen (Hohentwiel), located near Lake Constance, represents a population from a period of technological transition in southwestern Germany. The site contains several graves with metal artefacts that originated in other parts of Central and Western Europe, and therefore these could be interpreted as being the graves of non‐local individuals. The purpose of this study was to investigate this possibility through the application of isotopic analysis. The ratios of strontium and oxygen isotopes in human enamel reflect the geological origin of food and drinking water consumed during enamel formation in early life stages. Additionally, the ratio of sulphur isotopes from bone collagen reflects the origin of foods consumed during the last 10–20 years of life of an adult individual. We used these three isotope systems to attempt to identify local and non‐local individuals at the site. We found that the isotope ratios of Sr, O and S of the humans were relatively homogeneous and generally correspond to the isotope signature of the local geology, climate and environment. We conclude that the sampled population is of local origin and does not show patterns of individual mobility, even though there is evidence for long‐distance trade and exchange of the metal artefacts at this site.     

Variation in strontium isotope ratios of archaeological fauna in the Midwestern United States: a preliminary study

Strontium isotope values (⁸⁷Sr/⁸⁶Sr) in bone and tooth enamel have been used increasingly to identify non-local individuals within prehistoric human populations worldwide. Archaeological research in the Midwestern United States has increasingly highlighted the role of population movement in affecting interregional cultural change. However, the comparatively low level of geologic variation in the Midwestern United States might suggest a corresponding low level of strontium variation, and calls into question the sensitivity of strontium isotopes to identify non-local individuals in this region. Using strontium isotopes of archaeological fauna, we explore the degree of variability in strontium ratios across this region. Our results demonstrate measurable variation in strontium ratios and indicate the potential of strontium analysis for addressing questions of origin and population movement in the Midwestern United States.     

Lead isotope ratios: Tracking the migration of European‐Americans to Grafton,Illinois in the 19th century

Lead isotope analysis was conducted using inductively coupled plasma mass spectrometer (ICP‐MS) instruments on local soil samples and human premolar tooth enamel from a 19th century population from Grafton, Illinois, USA. The goal of the study was to determine if lead isotope analysis could be used to infer place of birth and patterns of 19th century migration into the city of Grafton. Five soil core samples from a location near Grafton, Illinois, five grave soil samples from the city cemetery and the tooth enamel of 19 human premolars were analysed. The results of the soil core analysis indicated that the lead isotopic signature of Grafton differs significantly from isotope ratios of other geographic areas associated with recorded places of birth of 19th century Jersey County residents. Elemental and isotope analysis of the soil samples indicated that diagenesis was not a factor in the analysis of lead isotopic signatures of enamel. From the lead isotope analysis of human premolars, the geographic origin of 13 of the remaining 15 individuals could be inferred. The inferred geographic origin was supplemented by an analysis of 1860 mortality and census records and demonstrated the utility of using lead isotope analysis in bio‐archaeological investigations. Copyright © 2010 John Wiley & Sons, Ltd.     

Evidence for “Celtic migrations”? Strontium isotope analysis at the early La Tène (LT B) cemeteries of Nebringen (Germany) and Monte Bibele (Italy)

Strontium isotope analysis on human remains from the Iron Age (4th/3rd century BC) cemeteries of Nebringen, Germany and Monte Bibele, Italy were carried out to investigate the role of residential changes during the period of the historic “Celtic migrations”. From an archaeological perspective, the location of the cemeteries in the Celtic core (Nebringen) and expansion area (Monte Bibele), and the distinctive development of their material culture, suggest that the buried populations had differing mobility rates. On the contrary, the strontium results indicate that only few individuals were mobile or non-local. There is, however, a difference in variation of strontium isotope ratios between the two studied cemeteries, presumably caused by differing geological conditions. In Nebringen changing use of cultivated land in a geologically heterogeneous environment most likely caused varying strontium isotope ratios even within the same jaw, while individual mobility over longer distances can also not be excluded. In Monte Bibele the range of strontium isotope ratios is narrow, and this may be explained by a community living in the same village and using the same agricultural resources. For various prehistoric time periods it has been suggested that some population groups were more mobile than others, for example because they had special social roles. In the two cemeteries studied here, males have slightly more often a non-local birthplace or moved during childhood. In contrast, females had isotope signatures which are more consistent with the local geological environment. Male mobility is, however, not correlated with burial as a warrior, and patrilocal residential patterns were not observed among females. Foreign and local items are found with isotopically local and non-local individuals and objects per se do not indicate descent. The motives for residential changes were, therefore, varied and not constrained to a specific area or population group. The presented dataset provides details on the way of life and land-use in the Celtic communities, for which information is otherwise absent.     

Palaeobiology of the Medieval Population of Albano (Rome,Italy): A Combined Morphological and Biomolecular Approach

Recent years have seen increased interest in skeletal populations from the Imperial Roman Age in Italy, but much less is known about diet and standards of living in the subsequent medieval period. To fill this gap, we conducted a morphological analysis of human remains from Albano, an Italian town near Rome, as well as a stable isotope analysis of bone collagen to reconstruct diet. The sample was recovered from a Medieval cemetery (1040–1220 cal. yr. BP) located in the gardens of the historical Palazzo Doria Pamphili in Albano. A minimum number of 40 individuals (31 adults and 9 sub‐adults) were examined using standard methods. Though the general health status of the population was good, signs of cribra orbitalia and diffuse enthesopathies were noted during the morphological examination. Stable carbon and nitrogen isotope analyses of the bone collagen from 24 adult humans and three faunal bones indicate that the diet of the population may be described as predominantly terrestrial and C₃‐plant based although the data for some of the individuals suggest a modest consumption of C₄‐(millet) based or aquatic proteins. No evidence of significant dietary differences between the sexes was found. The comparison of the isotope data from Albano with those from populations recovered in the same region is consistent with a shift from a terrestrial, possibly plant foods‐dominated subsistence in the Early Middle Ages to a diet with a higher contribution from animal proteins, both terrestrial and aquatic, in the Later Middle Ages. Copyright © 2013 John Wiley & Sons, Ltd.     

Strontium isotope evidence of migration and diet in relation to ritual tooth ablation: a case study from the Inariyama Jomon site,Japan

Ritual tooth ablation was extensively practiced among Jomon (Japanese Neolithic) societies in their final phase (ca. 3000-2300 BP). This tradition includes two different tooth ablation patterns, type 4I and type 2C, referring to extraction of the mandibular incisors and canines, respectively. However, the reason for this difference is unclear. Previous carbon and nitrogen stable isotope analysis of human remains from the Inariyama shell mound revealed that type 4I individuals were more dependent on terrestrial resources and type 2C individuals on marine resources. To test this hypothesis, we performed strontium (Sr) isotope (⁸⁷Sr/⁸⁶Sr) analyses on the same skeletal remains and on modern plants around the site. Because Sr isotope ratios of plants differ according to the local geology and seawater has a consistent Sr isotope ratio, the Sr isotope ratios of tooth enamel can reveal both migration and diet. Comparing Sr isotope ratios in plants and seawater with those of tooth enamel, we identified four possible immigrants. Type 4I locals had significantly higher Sr isotope ratios than type 2C locals. The ratios of the type 4I and type 2C locals were close to those of terrestrial plants and seawater, respectively, suggesting that type 4I locals had incorporated much Sr from terrestrial resources and type 2C locals from marine resources. These results support the hypothesis that ritual tooth ablation reflects dietary differences throughout an individual’s life, and they suggest possible occupational differentiation among the Jomon people.     

IDENTIFICATION OF LEAD SOURCES BY STABLE ISOTOPE RATIOS IN BONES AND LEAD FROM POUNDBURY CAMP, DORSET

Summary. Skeletal material from the Romano-British cemetery at Poundbury Camp on the outskirts of Dorchester is remarkable for the high concentration of lead detected in the bones. In an attempt to discover whether this lead was local in origin or had been introduced in imported commodities lead isotope ratios were determined for a selection of bones. The isotope ratios indicated at least two sources for the lead. Three of the individuals tested appear to have acquired lead derived from local, British, ores while the bone from the fourth individual, a child, had a lead isotope ratio identical to ore from Laurion in Greece. The isotope ratios are so close as to indicate immigration of the child rather than importation of lead in food or wine.     

Reconstruction of Socioeconomic Status in the Medieval (14th–15th Century) Population of Grevenmacher (Luxembourg) Based on Growth,Development and Diet

Numerous historical sources describe many aspects of the estate‐based society in medieval times; the detection of socioeconomic status within populations through skeletal remains is a topic of growing interest in anthropological studies. In medieval times, it was common for members of high social rank to be buried within or next to a church. This was certainly the case in Grevenmacher (Luxembourg), where remains of a church building and an adjacent cemetery from the 13th until the beginning of the 15th century were subjected to archaeological and anthropological analysis. By integrating archaeological and anthropological elements, as well as stable isotope analysis, we documented osteological manifestations as indicators of diet differentials in two subsamples (first group consists out of 56 individuals, second one out of 184 individuals) from the medieval cemetery of Grevenmacher. We could distinguish two subsamples that we assumed as different in their socioeconomic status with regards to the burials' position in the cemetery and burial characteristics. Differences in osteological traits such as bone length, stature and body mass indicated differences in diet between sample groups. To substantiate these outcomes, stable isotopic analysis (δ¹³C, δ¹⁵N) were made; the results displayed a clear separation between the sample groups on the basis of their diet. Therefore, we were able to confirm certain individuals in the medieval population of Grevenmacher in relationship to their socioeconomic status. Copyright © 2017 John Wiley & Sons, Ltd.     

Sulphur isotope evidence for freshwater fish consumption: a case study from the Danube Gorges,SE Europe

To explore the use of sulphur isotopes as an indicator of the consumption of freshwater fish, we undertook sulphur isotope analysis on bone collagen extracted from humans and animals from five archaeological sites from the Danube Gorges region dating from the Mesolithic to the middle Neolithic periods. The results show a difference in the sulphur isotope values between freshwater and terrestrial ecosystems of 8.7‰. To reconstruct human diets, bone collagen from 24 individuals was analysed for carbon, nitrogen and sulphur isotopic values. The nitrogen isotope ratios ranged from 10.3 to 16.5‰ and the carbon isotope ratios ranged from −20.8 to −18.3‰. Low nitrogen isotope values were found for individuals with low sulphur isotope ratios reflecting the low sulphur isotopic values of the terrestrial animals. The highest human nitrogen isotope values coincided with higher sulphur isotope ratios, which are related to the higher sulphur isotope values of the freshwater fish. Intermediate human sulphur isotopic ratios between these two extremes showed mixed diets of both terrestrial and freshwater resources.     

Status and gender differences in diet at Mound 72, Cahokia,revealed by isotopic analysis of bone

Cahokia Mound 72 contains 272 human burials dating to the Lohmann and early Stirling phases (ca. 1050–1150 AD) of the Mississippian period. Substantial status- and gender-related differences in burial style are apparent. Some burials are associated with large quantities of prestigious grave goods, suggesting high status. Mass graves of young adult females with skeletal indicators of poor health suggest low status and nutritional stress. Nitrogen isotope ratios of bone collagen show that high status individuals ate much more animal protein, but carbon isotope ratios of collagen suggest these individuals ate only ca. 10% less maize than lower status individuals. Apatite carbon isotopes show low status females ate ca. 60% more maize than high status individuals, which confirms the large nitrogen isotope difference of females in mass graves. These results indicate high and low status individuals had significantly different diet compositions and nutritional qualities. The stable isotope evidence supports paleopathological data for status-related differences in health, and dental morphological data for presumed genetic differences in origin. These data also provide insights into the nutrition- and health-related dimension of regional hierarchical organization of settlements and social inequality of this complex chiefdom in the greater Cahokia region.     

Stable isotopic evidence for diet in a Roman and Late Roman population from Leptiminus,Tunisia

This study investigates the diet of the Roman and Late Roman population of Leptiminus on the Mediterranean coast of Tunisia. Stable carbon and nitrogen isotope analysis of human bone collagen and carbonate samples obtained from individuals buried in four cemeteries at Leptiminus was conducted in order to reconstruct the diet of the population, investigate the relative importance of marine vs. terrestrial resources, explore sex-, age-, and status-based variations in diet, examine temporal changes in the types of foods consumed, and compare the diet at Leptiminus with that of other Roman populations. The results of this study indicate that the residents of Leptiminus consumed a diet that was heavily reliant on terrestrial plant resources with the addition of a significant amount of marine resources. There were no significant sex differences in isotope values. In contrast, distinct dietary differences were seen between the adults and children. Nitrogen isotope values suggest that weaning began before the age of two and was completed by about 3 years of age, a finding consistent with previous isotopic studies of Roman samples. A temporal shift in diet is suggested by the nitrogen isotope values measured in samples from the most recent cemetery. A comparison of the data from Leptiminus with that derived from other Roman sites indicates that regional variability in diet existed within the Empire.     

Mobility or migration: a case study from the Neolithic settlement of Nieder-Mörlen (Hessen,Germany)

A combination of stable carbon and nitrogen isotope analysis of collagen and radiogenic strontium isotope analysis of tooth enamel was used to investigate diet and mobility at the early Neolithic settlement of Nieder-Mörlen in Germany. The carbon and nitrogen ratios suggest a mixed terrestrial based diet that is consistent with data previously published for early Neolithic sites in Europe. The strontium isotope data indicate a high degree of human mobility with only one individual having an isotope ratio consistent with locally derived strontium. Unusually, a group of non-local juveniles with isotope ratios typical of upland regions is also present at the settlement but there are no adult burials with such values. Whilst transhumance is considered as an explanation, it would not explain why these non-local juveniles lived foreshortened lives and other possible mechanisms are therefore discussed.