Early Processed Triticeae Food Remains in The Yanghai Tombs,Xinjiang, China

Visible food remains can provide evidence regarding ancient food processing, the spread of cereals and cultural communication. Some desiccated food remains were discovered in the Yanghai Tombs, Turpan district, in Xinjiang, China (2600–2900 bp ). These food remains were analysed by Fourier transform infrared (FT–IR) spectroscopy combined with plant microfossils, including starch grains and cross cells of pericarp from the cereal bran fragments. The results showed that these food remains were cooked dough food made from wheat (Triticum aestivum) and barley (Hordeum spp.). The cross‐sections of these remains look very dense, not porous under a microscope, which suggests that no fermentation had happened, so these foodstuffs may be some kind of flatbread. Although wheat and barley had been introduced into China by at least the third millennium bc , these remains are still the earliest known direct evidence that wheat and barley were ground into flour and then processed as foodstuffs in north‐western China.     

Evidence for early viticulture in China: proof of a grapevine (Vitis vinifera L., Vitaceae) in the Yanghai Tombs,Xinjiang

A stem was discovered in the Yanghai Tombs, Turpan District in Xinjiang, China. Anatomical features showed it to be of grape (Vitis vinifera L.). Radiocarbon dating indicates it to be nearly 2300 years old, which would suggest that there was grape cultivation at least from that time. To date, this is the earliest physical evidence of V. vinifera cultivation in China.     

Identification of ancient starch grains from the tribe Triticeae in the North China Plain

Both macrofossil and microfossil plant remains from the grass family (Poaceae) have been recovered from Neolithic and historic sites in China. Basing our work on the plant taxa that were previously recovered, we analyzed the economically significant genera for modern starch grain analysis with a focus on the important tribe Triticeae. Modern starch grains from the tribe Triticeae were compared with those from other grasses, and criteria for identification were determined. In total, 38 species within 28 genera, 13 tribes and 4 subfamilies were selected for analysis. Results demonstrate that starch grains from members of the tribe Triticeae are discernible from those of other tribes by their distinctive lenticular morphologies and surficial pressure craters. A dichotomous key covering 10 species within 7 genera of the Triticeae was created, thus allowing identification of members of the tribe to the level of genus. Application of the dichotomous key to the ancient starch assemblage recovered from lithic tools excavated from the early Neolithic site of Donghulin demonstrates that plants from the genera Hordeum and Agropyron were exploited alongside millets at this site.     

NEW EVIDENCE FOR EARLY SILK IN THE INDUS CIVILIZATION*

Silk is an important economic fibre, and is generally considered to have been the exclusive cultural heritage of China. Silk weaving is evident from the Shang period c. 1600–1045 bc , though the earliest evidence for silk textiles in ancient China may date to as much as a millennium earlier. Recent microscopic analysis of archaeological thread fragments found inside copper‐alloy ornaments from Harappa and steatite beads from Chanhu‐daro, two important Indus sites, have yielded silk fibres, dating to c. 2450–2000 bc . This study offers the earliest evidence in the world for any silk outside China, and is roughly contemporaneous with the earliest Chinese evidence for silk. This important new finding brings into question the traditional historical notion of sericulture as being an exclusively Chinese invention.     

Molecular history of tuberculosis from ancient mummies and skeletons

The origin and evolution of the infectious disease tuberculosis (TB) and its pathogens is still not fully understood. An important effort for a better understanding of the underlying mechanisms of TB evolution lies within the investigation of skeletal and mummified material dating back several thousand of years. In this work, molecular data from mummified and skeletal material from different time periods of the Old World are compared, and the current status of ancient mycobacterial DNA analysis in ancient human remains is discussed, with particular reference to the genetic evolution of human TB. The molecular analysis of material from southern Germany (1400–1800 AD), Hungary (600–1700 AD) and Egypt (3500–500 BC) revealed high frequencies of TB in all time periods. In several individuals from ancient Egypt the mycobacterial DNA could be further characterised by spoligotyping. Thereby, evidence for ancestral M. tuberculosis strains was found in the pre‐ to early dynastic material from Abydos (3500–2650 BC), while typical M. africanum signatures were detected in the Middle Kingdom tomb in Thebes‐West (2050–1650 BC). Samples from the New Kingdom to Late Period tombs (1500–500 BC) were characterised as modern M. tuberculosis strains. In concordance with other studies on ancient skeletal and mummified samples, no evidence for the presence of M. bovis was found. These results contradict the theory that M. tuberculosis evolved from M. bovis during domestication, but supports the new scenario that M. tuberculosis probably derived from an ancestral progenitor strain. Copyright © 2007 John Wiley & Sons, Ltd.     

An Analysis of the Gut Contents of Five Well-Preserved Human Bodies from Tarapacá, Northern Chile

The detailed microscopic analysis of the gut contents from five well-preserved desiccated human bodies, dated between 540 BC and AD 600, from the Tarapacá quebrada, Northern Chile, has been undertaken. This analysis indicated that local wild resources such as the seeds of Chenopodium sp., pods of Prosopis sp. and the underground organs of Schoenoplectus/Scirpus sp. made up a substantial part of the diet even after the presence of domesticated species can be demonstrated for the area. Evidence for the consumption of fish was also recovered but the identification of larger mammals such as the Andean camelids in the diet is only tentative. This stands in contrast to the data recovered from other classes of archaeological remains and highlights the need to correlate as many different classes of complementary data as possible if a more complete picture of ancient diet is to be forthcoming.     

Analysis of Plant Microfossils in Early European Latrine Fills from Russell,Northern New Zealand

Microfossil analysis of latrine fills from the early European (1830s) settlement of Russell, northern New Zealand, provides direct evidence for diet. Microfossils identified include pollen of maize (Zea mays), Brassicaceae (e.g., mustard, broccoli), Allium type (e.g., onion) and mint (Mentha), and starch grains of maize and potato (Solanum tuberosum). Wetland microfossils (pollen and algal spores) provide clues to source and quality of drinking water.     

Prehistoric wooden bows and arrows in the Turpan Basin,Northwest China: Wood selection and utilization in a mosaic landscape

In the present study, we analyzed seven wooden bows and 13 arrows excavated from the cemeteries of Subeixi Culture (13th century BCE–AD second century) in the Turpan Basin, northwest China. A total of six taxa, viz. Morus sp., Salix sp., Picea sp., Lonicera sp., Betula sp., and Tamarix sp. were identified. Some of these taxa have been generally accepted to be suitable for bow-and-arrow production. All these taxa were local in origin but distributed in different ecotonal zones, including oases, saline-alkali lands, and mountain areas. Previous archaeological studies have confirmed that the Subeixi populations lived an agro-pastoral life. By analyzing the possible provenance of these wood samples, it can be hypothesized that these ancient agro-pastoralists moved between the Turpan Basin and the Tianshan Mountains on a seasonal basis.     

新疆阿斯塔那唐墓出土彩塑的制作工艺和颜料分析

新疆阿斯塔那古墓群出土了大量的彩塑,为了解其制作工艺与彩绘颜料成分,采用显微激光拉曼光谱,结合剖面观察和能量色散X射线荧光光谱等科技手段,对出土于唐墓的6个样品进行了分析鉴定.结果表明,泥胎表面先用硬石膏做打底层,再施以彩绘,且彩绘颜料厚薄有异.所使用的红、黄、黑、白和绿色颜料有铅丹、密陀僧、朱砂和土红、雌黄、炭黑、硬石膏及氯铜矿等无机颜料,而粉色彩绘是由铅丹(或铅丹和密陀僧的混合物)与硬石膏调和而成,且密陀僧作为彩绘颜料在新疆是较早的发现.其最为重要的发现是蓝色彩绘为植物性染料——靛蓝.这一研究结果,为进一步的彩塑修复与保护方案的制订提供了有价值参考.     

The effect of charring and burial on the biochemical composition of cereal grains: investigating the integrity of archaeological plant material

Stable isotope analysis of charred archaeobotanical cereal grains has the potential to provide direct evidence of crop growing conditions in the past and to refine palaeodietary predictions. If isotope values of archaeobotanical material are to be considered robust, it is necessary to characterise the compositional changes associated with their charring and burial. This study used a suite of analytical techniques, including FT-IR and solid state ¹³C NMR, to characterise changes in the biochemical composition of modern einkorn grains with heating at 230 °C for 2 h, 4 h, 8 h and 24 h, encompassing conditions that replicate their undistorted ancient counterparts. The biochemical composition of archaeobotanical charred einkorn grains was also investigated by FT-IR and solid state ¹³C NMR in order to assess the changes in composition which occur during burial. Results of FT-IR and solid-state ¹³C NMR show that heating of modern einkorn grains resulted in Maillard reactions between cereal proteins and starch, forming high molecular weight melanoidins, which contain both alkyl and aromatic carbon. Loss of low molecular weight carbon and nitrogen-containing volatiles resulted in a slight but non-systematic increase in the δ¹³C values and a systematic increase of 0.8‰ in the δ¹⁵N values of the charred einkorn grains. Solid-state ¹³C NMR shows that the ancient charred einkorn grains consisted entirely of aromatic carbon and retained a similar proportion of nitrogen to their modern 24 h charred counterparts, despite a significantly lower concentration of amino acids. This indicates that the amino acid nitrogen in the ancient charred grains was retained in the stable melanoidins whose polymeric structure makes them resistant to subsequent degradation.     

Influence of grinding on the preservation of starch grains from rice

China is a major centre for rice domestication, where starch grain analysis has been widely applied to archaeological grinding tools to gain information about plant use by ancient Chinese societies. However, few rice starch grains have been identified to date. To understand this apparent scarcity of starch grains from rice, dry- and wet-grinding experiments with stone tools were carried out on four types of cereals: rice (Oryza sativa L.), foxtail millet (Setaria italica), Job's tears (Coix lacryma-jobi L.) and barley (Hordeum vulgare L.). The results reveal that dry-grinding produces significant damage to starches to the point where they may be undetected in archaeological samples, while wet-grinding causes only slight morphological changes to the starch grains. Moreover, rice starch grains have the most substantial alterations from dry-grinding, possibly impeding their identification. These findings provide a possible means to explain the relative scarcity of rice starch grains recovered from archaeological grinding tools, which it is suggested was caused by the use of the dry-grinding technique. Therefore, it is suggested that rice starch grains have been likely underrepresented in the archaeological record, and previous interpretations of starch analyses need to be reconsidered.     

Investigation of ancient noodles,cakes, and millet at the Subeixi Site,Xinjiang, China

Ancient foodstuffs, including noodles, cakes, and common millet, were excavated from the Subeixi Cemeteries (cal. 500–300 years BC), Turpan District in Xinjiang, China. Starch grain and phytolith analyses were undertaken to identify the plant species involved. These indicate that the noodles and cakes were made from Panicum miliaceum. Ancient food preparation technologies were also investigated by cooking experiments.     

The earliest evidence of domesticated wheat in the Crimea at Chalcolithic Ardych-Burun

Abstract

This paper reports the discovery of the earliest evidence of domesticated wheat in the Crimean peninsula from the Ardych-Burun shell midden site, Ukraine. The Ardych-Burun site dates to middle of the 4th millennium cal b.c. For the first time, the chronology of a Ukrainian Chalcolithic period site has been established through direct radiocarbon dating of cereal grains retrieved from it. This discovery allows for a wider discussion of the chronology and geographical origins of domesticated plant species in Ukraine and the role the Caucasian corridor may have played in the spread of agriculture into eastern Europe. The presence of cereal crops in the southern Crimea enriches our understanding of the subsistence strategies of the coastal population, which was previously linked only with pastoralism, hunting, and the exploitation of marine resources.     

Silicified wood of Early Miocene Nothofagus,Acacia and Myrtaceae (aff. Eucalyptus B) from the upper Lachlan valley,New South Wales

Three specimens of silicified wood, two transported and one probably in situ, have been found in association with Early Miocene basaltic lavas in the upper Lachlan valley, N.S.W. On the basis of their preserved structure, the three specimens have been identified as belonging to Nothofagus, Acacia, and the family Myrtaceae, respectively. The specimen of Nothofagus (one of the transported specimens) constitutes the first identification of a macrofossil of this genus from this interval in southeastern mainland Australia, while its conjunction with the myrtaceous specimens (one probably in situ) may indicate vegetation zonation according to elevation, as proposed on pollen evidence from Kiandra. Such zonation is not demanded by the upper Lachlan evidence, however, but if it is favoured, reconstruction of the Early Miocene geography of the upper Lachlan constrains the maximum elevation above the locality of the myrtaceous fossils to be 350 m.     

Lichenometric dating and the nature of the excavation of the Huashan Grottoes,East China

The present study aims to identify the timing of the Huashan Grottoes' excavations using the lichenometric dating method. A total of 96 maximum inscribed circle diameters of Rhizocarpon geographicum growing on the ancient architectures and the walls of entrances are measured in the Huashan Grottoes area of southern Anhui, East China. The results show that the period of the grottoes' excavation is estimated to be 450–335 yr BP, or the middle and late Ming Dynasty (1552–1667 AD). The lichenometric dating results are also validated by a lithologic comparison of the grottoes and the surrounding ancient architectures, as well as by the studies on the historical documents and the fragments of ancient porcelain in the grottoes. Furthermore, we infer that the excavation of the inner grottoes might have lasted till the Qing Dynasty (1636–1912 AD), because the lichens chosen for dating are mainly at the grottoes' entrances. The wider significance of this work is that it demonstrates, for the first time, lichenometric dating can be applied in the historical studies on the ancient architectures in East China with a relatively warm and humid climate.     

Vegetation,land, and wood use at the sites of Bat and Al‐Khashbah in Oman (fourth–third millennium BC)

About 4200 charcoal fragments have been identified from the fourth‐ to third‐millennium BC archaeological sites of Bat and Al‐Khashbah in order to gain an understanding of plant resources available at the sites. Acacia sp., Ziziphus sp., and Tamarix sp. were the main taxa identified at both sites and indicate a similar vegetation composition as today. Phoenix sp. (date palm) charcoal also has been found at both sites. Whereas the cultivation of date palm for the 2700–2300 BC layers from Bat was likely, given other circumstantial evidence (i.e. local cereal cultivation and floodwater irrigation), it is unclear whether date palm was cultivated at Al‐Khashbah. Especially for the older periods (3300–2700 BC) it is possible that nomadic pastoralists were exploiting and/or managing wild date palms. The find of Avicennia marina at Al‐Khashbah indicates long‐distance contacts with the coast.     

Wild or domesticated: DNA analysis of ancient water buffalo remains from north China

Recent zooarchaeological studies on water buffalo (Bubalus sp.) remains from China and south Asia question the traditional view that water buffalo were first domesticated in Neolithic China over 7000 years ago. The results from several recent population genetic studies of modern domesticated buffalo (Bubalus bubalis) are not consistent with each other, placing the original center of buffalo's domestication in south Asia, southeast Asia, or China. This paper reports a study using an ancient DNA approach to analyze water buffalo remains from Neolithic sites in north China to investigate their affinities with modern domesticated water buffalo, and to shed light on the origin of modern domesticated water buffalo in China.A 169 bp fragment of D-loop mitochondrial DNA was successfully amplified and verified for 13 of 24 bone samples obtained from seven archaeological sites along the Wei River valley in Shaanxi Province, China. The bone samples which yielded positive DNA can be dated to 8000–3600 cal. BP. The phylogenetic analysis of the obtained DNA sequences along with modern water buffalo sequences indicated that the ancient water buffalos were not the direct ancestor of modern domesticated water buffalo. However, the phylogenetic analysis, along with BLAST searches of these ancient DNA sequences, did demonstrate their relatedness to water buffalo more so than to any other bovid species, confirming the existence of indigenous wild (but now extinct) water buffalo species (B. mephistopheles) in ancient China.The DNA analysis of these ancient remains failed to establish direct links between modern domesticated water buffalo (B. bubalis) and indigenous water buffalo (B. mephistopheles) from ancient China. If further DNA studies of more ancient remains from other regions of China confirm the observation of solely indigenous water buffalo species in ancient China, it would suggest modern water buffalo might not have been first domesticated in China.     

Identification of PbO (BaO) faience from an early and middle Warring States period cemetery at Zhaitouhe,northern Shaanxi,China

Low‐temperature vitreous materials fluxed with lead (and barium) appeared in the early Warring States period (c.fifth century bce ) in ancient China. A range of lead vitreous products fluxed with the same agent, including faience/glass beads, glazed pottery and Chinese blue (purple) pigment, were developed subsequently. This study carries out scientific analysis of six vitreous beads unearthed from the Zhaitouhe cemetery site in northern Shaanxi dating to the early and middle Warring States period to investigate their chemical composition, microstructure and coloration. The lead (and barium) vitreous beads identified here are some of the earliest lead vitreous materials discovered in China so far, and therefore important for the study of the development of lead vitreous technologies in ancient China. This paper also points out that it is meaningful to evaluate the lead glass, glazed pottery and barium copper silicate pigments as a whole technological assemblage given their close relationships.     

Late Pleistocene to Early Holocene Plant Movements in Southern Kyushu, Japan

From the late Pleistocene to early Holocene in Japan, subtropical and temperate forest elements moved northwards. This affected human choices and access to food sources. More settled patterns of living spread northwards gradually, and northern hunting-gathering-fishing people began cultivating vegetables and cereal crops. This poster reports the presence of ancient starch residues on stone artefacts in Kagoshima Prefecture, southern Kyushu. The oldest residues recovered are dated by context to about 30,000 ¹⁴C yr BP. If such residues can be identified, it may be possible to detect a hypothesized early phase of tropical plant movement northwards during warmer climate peaks in the late terminal Pleistocene, as well as during the long period of Holocene warming that followed. As an initial step towards identification, the morphological characteristics and condition of the starch granules are described and compared to those of other sites in early Japan.     

Late Stone Age subsistence in the Tilemsi Valley, Mali: Stable isotope analysis of human and animal remains from the site of Karkarichinkat Nord (KN05) and Karkarichinkat Sud (KS05)

The pathways leading to the adoption of cereal cultivation and pastoralism in West Africa are poorly understood. In order to elucidate the transition to food production during the Late Stone Age in Mali’s Tilemsi Valley samples of ancient and modern human and animal remains were selected for carbon and oxygen isotope analysis. Our results indicate the inhabitants of Karkarichinkat Nord (KN05) consumed considerable quantities (85%) of carbon derived from C₄ plants, either directly in the form of C₄ grasses such as wild Panicum sp. and possibly domestic Pennisetum sp. or indirectly through the consumption of C₄ grazers such as Bos sp. and Ovis sp.