Adding Hydrogen to the Isotopic Inventory—Combining δ13C, δ15N and δ2H Stable Isotope Analysis for Palaeodietary Purposes on Archaeological Bone

Few papers using hydrogen stable isotope analysis for human palaeodietary reconstruction purposes have been published and the usefulness of this additional dietary indicator is highlighted here. The hydrogen stable isotope results provide evidence for the continued exploitation of aquatic resources throughout the prehistory of the Limfjord area in Denmark, which is supported by FRUITS estimates using three (CNH) isotopic proxies. While aquatic dietary input has been identified in Mesolithic and Viking Age individuals before, our results show that, in fact, this continued throughout the periods in between (Neolithic, Bronze and Iron Age), albeit on a small scale.     

Dietary changes and millet consumption in northern France at the end of Prehistory: Evidence from archaeobotanical and stable isotope data

Diachronic changes of dietary human habits between the Neolithic and the Bronze Age are mainly identified through archaeological artefacts and archaeozoological and archaeobotanical studies. This paper aims to demonstrate the importance of a multi-disciplinary approach for palaeodietary studies and to identify the food changes between Neolithic and Bronze Age human groups in northern France. These changes are probably linked to the introduction of new crops, such as millet, and the use of stable isotope analysis on bones and teeth proves to be an effective method for assessing the role of this specific cereal in the diet and the economy. Stable isotope analyses were performed on bone and tooth collagen and apatite from eight humans and five domestic animals from a Late Bronze Age site (LBA; Barbuise; 15th–13th c. BC; Aube). The studied corpus is compared with isotopic data from human and animal bones from a nearby Neolithic site (Gurgy; 5th mill. BC; Yonne) and regional Neolithic to Iron sites located in northern France. Moreover, Barbuise data are supplemented by information from an important archaeobotanical study carried out on 21 LBA and Early Iron Age sites in the region. Neolithic and LBA human collagen isotopic ratios (δ¹³C, δ¹⁵N) differ statistically, as do those of some animals. Carbon isotopic ratios of human apatite corroborate collagen results indicating the consumption of ¹³C enriched food by LBA humans and animals compared to Neolithic samples. The high number of occurrences of plant remains in the Bronze Age settlements near the site points to the consumption of C₄ plants, such as millet, and would account for these results.     

Diet Variability and Stable Isotope Analyses: Looking for Variables Within the Late Neolithic and Iron Age Human Groups from Gougenheim Site and Surrounding Areas (Alsace,France)

Several human groups (from the Neolithic to the Bronze Age) have been analysed in France during the past decade (mainly for C and N stable isotope) as part of research programmes focusing on prehistoric dietary variability. The environment, cultural/social choices or even biological characteristics are among the parameters influencing food acquisition and consumption. This short report presents the first diachronic isotopic results on the palaeodiet in northeastern France. Because of the exceptional archaeological characteristics (human deposits in various positions in pits) of the bone collection from the site of Gougenheim and the surrounding areas (Late Neolithic‐Iron Age, Alsace, France), this assemblage provides a new isotopic dataset to study diet and the potential relationship with social elements or other factors involved in food choices. In order to obtain individual palaeodietary information, carbon and nitrogen stable isotope analyses were performed on 23 adults and 20 immature human bone collagen samples as well as on 25 animal remains. Data were then combined with zooarchaeological and anthropological/archaeological results to reconstruct part of the dietary pattern (i.e. protein consumed) and to detect possible links between the deposit and individual or group social status, defined here by specific mortuary practices. For the Late Neolithic period, isotopic values show, among other things, a wide δ¹³C range within the female human group, which is statistically lower than the male one. Women probably consumed more diversified food sources, suggesting increased residential mobility. Although body deposits point to the presence of two distinct subgroups, no relationship with animal protein intake was identified. Moreover, the comparison with Iron Age individuals brought to light different dietary patterns between the two periods, indicating that stable isotope values were affected throughout time either by increased millet/legume consumption or environmental/anthropic changes. Copyright © 2014 John Wiley & Sons, Ltd.     

Stable isotope analysis of humans from Xiaojingshan site: implications for understanding the origin of millet agriculture in China

Millet agriculture originated in Northern China in the early Neolithic period (ca. 8000 BP), however, the actual importance of millet in human diets is still not clear. To determine the relative contribution of millet in human diets in this period we undertook stable isotope analysis of humans from Xiaojingshan site and fauna from Yuezhuang site, both of which are attributed to the Houli Culture and date to about 8000 years ago. The carbon isotope values of human bone collagen showed that millet (as a C₄ plant) only contributed approximately 25% of dietary protein, with the rest from C₃ based plant and animal sources, if a simple mixing model is used. We did not observe any statistical dietary difference between males and females at the site, although it has been argued that the Houli Culture was a matriarchal society. Finally, we compared our data with other published isotopic data from the contemporary Jiahu site and Xinglongwa site and a number of sites from the subsequent Yangshao Culture and found that millet only became a significant source of dietary protein approximately 1000 years later, as human carbon isotope values from these later sites indicated that almost all of dietary protein came from C₄ (i.e. millet) sources.     

Coast–inland mobility and diet in the Danish Mesolithic and Neolithic: evidence from stable isotope values of humans and dogs

Here we present the stable isotope ratios of carbon and nitrogen measured in bone collagen extracted from humans, dogs, herbivores and fish from Mesolithic and Neolithic coastal and inland sites in Denmark. Bones of freshwater fish from several Early Mesolithic lake-side sites have δ¹³C values surprisingly similar to those seen in marine fish. We propose a model, based on δ¹³C and δ¹⁵N, for the correction for both marine and hard water reservoir effect in radiocarbon dates. A strong reliance on aquatic protein is demonstrated for the Mesolithic inhabitants of the region from the middle of the Early Mesolithic onwards. A significant part of the protein in the diets of the dogs and humans from the Middle and Late Mesolithic was of marine origin, even at inland sites. This observation points to a high degree of (seasonal) coast-inland mobility. The isotopic evidence indicates that during the Neolithic small quantities of aquatic foods were still common sources of dietary protein.     

Palaeodiet of Mesolithic and Neolithic populations of Meuse Basin (Belgium): evidence from stable isotopes

The human diet was investigated using the carbon and nitrogen isotopic signatures of 93 Mesolithic and Neolithic specimens (∼10,000–2000 BC) from the Meuse Basin (Belgium). During the Ancient Mesolithic period (∼9300–8000 BC), the environment was generally open and the main dietary protein was provided by hunted terrestrial mammals, with the possible addition of freshwater resources. Human remains are not available in the Meuse Basin from around 8000 BC to 4300 BC, thus preventing the study of the Mesolithic-Neolithic dietary transition in this region. Throughout the Middle Neolithic (∼4300–3000 BC), hunting was more difficult and less productive due to a densely forested environment. The contribution of freshwater resources to the diet increased, with the remaining proteins provided by terrestrial wild and/or domestic mammals, indicating that non-agricultural resources were not eliminated in this region during the Middle Neolithic period. The contribution of freshwater resources seems negligible in the Middle/Late and Late Neolithic periods (∼3300–1700 BC), with isotopic results revealing a diet composed of agricultural products. The δ¹⁵N values of infants are compatible with a weaning age at around 2 years. This study documents the dietary changes that occurred in Belgium during the first part of the Holocene, and reveals the subtle dietary distinction between wild and domestic terrestrial resources and freshwater resources.     

Stable isotope evidence for palaeodiets in southern Turkmenistan during Historical period and Iron Age

The subsistence patterns of Iron Age and Historical period humans from south-western Turkmenistan have been reconstructed using the carbon and nitrogen isotopic compositions of archaeological faunal and human bones. A qualitative comparison of the isotopic signatures points to a small proportion of ruminant meat and dairy in human diet for both periods. The ranges of proportions of dietary items yielded by a quantitative approach based on concentration dependent mixing models confirm the high proportions of plant food in the average diet, and show little change in the reconstructed diet for both periods. A comparison of results from zooarchaeological and isotopic approaches illustrates their complementarity in subsistence patterns reconstruction.     

Investigation of palaeodiet in the North Caucasus (South Russia) Bronze Age using stable isotope analysis and AMS dating of human and animal bones

This paper addresses the question of human palaeodietary adaptation in the Eneolithic and Early Bronze Age periods of the North Caucasus (South Russia) using stable isotope analysis. One of the key questions is the presence of fish in the diet. AMS radiocarbon dating of archaeological bone collagen has also been carried out to investigate potential radiocarbon reservoir effects in human skeletal material as a result of an aquatic diet. A fish component has previously been suggested in the diet of Iron Age and Bronze Age humans across Eurasia by isotopic research and radiocarbon dating of contemporaneous human and animal bones. In the North Caucasus however, isotopic data is scant. This study presents a new set of stable isotopic data from several Early Bronze Age sites, mainly belonging to the Maikop culture of the North Caucasus. The results show that the diversity in climate and environment across the northern Caucasus may be a causal factor for the patterns observed in the stable isotope values of terrestrial herbivores. This affects the isotopic values of the humans consuming them. The differences in δ¹⁵N and δ¹³C ranges of both humans and fauna were found to correlate strongly with geography and climate; the most enriched isotopic values are found in the dry steppe areas to the north. Overall, a relatively high enrichment in δ¹⁵N values of humans compared to local terrestrial herbivores and carnivores was observed. This indicates that aquatic resources were probably part of the Bronze Age diet in the region although the extent of this needs further investigation. The dramatic effect of environmental factors on isotopic values in the Early Bronze Age of the North Caucasus illustrates how confident conclusions cannot be drawn on the basis of a small number of samples from widely differing regions and time periods. Radiocarbon dating can provide a useful tool for identifying dietary derived reservoir ages in humans, potentially caused by a fish diet. With two possible exceptions, the nine human–animal bone pair dated as part of this study showed no consistent indication for a consistent reservoir effect.     

Diet uniformity at an early farming community in northwest Anatolia (Turkey): carbon and nitrogen isotope studies of bone collagen at Aktopraklık

Aktoprakl?k is a settlement site composed of three areas (A–C) in the Marmara region of northwest Anatolia, with phases of occupation that date to the Late Neolithic and Early Chalcolithic periods, mid-seventh to mid-sixth millennium bc (ca. 6400–5600 cal. bc). Here, we present 54 human and fauna bone collagen stable isotope results from the site, alongside five modern fish bone collagen isotope results, to examine the nature of human diet. The stable isotope analysis shows that human diet comprised the consumption of select C₃ terrestrial resources, with a preference for domestic animal proteins over plant proteins. The evidence to date suggests that animal husbandry was at the forefront of Late Neolithic and Early Chalcolithic subsistence practices. No isotopic difference in humans is observed between biological sex or between areas B and C at the settlement.     

A palaeodietary investigation of carbon (C/C) and nitrogen (N/N) in human and faunal bones from the Copper Age cemeteries of Varna I and Durankulak,Bulgaria

Stable isotope analyses have been applied to human and faunal bone collagen from the Varna I and Durankulak cemeteries to explore palaeodietary adaptations in the Neolithic and Eneolithic (Copper Age). The results suggest both populations primarily utilised terrestrial, C₃-based diets, despite their proximity to the Black Sea. The wider δ¹⁵N range of the Durankulak humans likely indicates the differential utilisation of terrestrial meat sources, which is probably related to the degree to which primary and/or secondary ovicaprid products were consumed, particularly since ovicaprid δ¹⁵N values differ from other herbivores. The isotopic distribution of Varna I reflects a linear relationship between δ¹⁵N and δ¹³C, suggesting that a minority of individuals enriched in both isotopic parameters supplemented their diets with marine resources. These burials include the well known ‘chieftain’ (burial 43) and show notable material wealth by way of grave goods. At the population level, however, there is no significant correlation between stable isotope values and material wealth at Varna I, a fact with implications for theories regarding emergent social/economic hierarchies in Balkan prehistory. Five burials at Durankulak were found to have relatively enriched δ¹³C and δ¹⁵N values with respect to the rest of the population. These burials reflect a prominently marine-based or mixed terrestrial C₃-based diet that included C₄ inputs, possibly from millet, for which the limitations of stable isotope analysis on bulk collagen are not able to differentiate. AMS dating has shown that these burials belong to a much later period.     

Brackish water foraging: isotopic landscapes and dietary reconstruction in Suisun Marsh,Central California

While bone stable isotope signatures of humans foraging in open coastal and fully terrestrial environments have been relatively well documented, the signature of foraging near brackish-water estuaries is less well known. This study examines the Central California isotopic landscape, or isoscape, showing how foods in different ecosystems have distinctive isotopic compositions. We present new bone collagen and apatite stable isotope data from two Late Holocene sites (CA-SOL-11 and CA-SOL-69) near Suisun Marsh, a low salinity brackish water estuary upstream from San Francisco Bay. Faunal remains at the sites include significant numbers of aquatic resources including fish and shellfish, in addition to terrestrial mammals, while paleobotanical remains suggest mainly terrestrial nuts and seeds. Stable isotope data at the population level corroborate this finding and show consumption of significant levels of brackish water nutrients, including both dietary protein and dietary carbohydrates and fats, paired with terrestrial foods. This dietary focus generates a unique brackish-water isotopic signature that is distinct from others in Central California. The documentation of an isoscape with strong regional gradients can be of tremendous help in reconstructing ancient patterns in subsistence, migration, and trade.     

Ancient DNA insights from the Middle Neolithic in Germany

Genetic studies of Neolithic groups in central Europe have provided insights into the demographic processes that have occurred during the initial transition to agriculture as well as in later Neolithic contexts. While distinct genetic patterns between indigenous hunter-gatherers and Neolithic farmers in Europe have been observed, it is still under discussion how the genetic diversity changed during the 5,000-year span of the Neolithic period. In order to investigate genetic patterns after the earliest farming communities, we carried out an ancient mitochondrial DNA (mtDNA) analysis of 34 individuals from Wittmar, Germany representing three different Neolithic farming groups (ca. 5,200–4,300 cal bc) including Rössen societies. Ancient DNA analysis was successful for six individuals associated with the Middle Neolithic Rössen and observed haplotypes were assigned to mtDNA haplogroups H5, HV0, U5, and K. Our results offer perspectives on the genetic composition of individuals associated with the Rössen culture at Wittmar and permit insights into genetic landscapes in central Europe at a time when regional groups first emerged during the Middle Neolithic.     

AN INVESTIGATION INTO DIET AT THE SITE OF YARNTON, OXFORDSHIRE, USING STABLE CARBON AND NITROGEN ISOTOPES

This paper reports the results of stable isotopic analyses conducted upon animal and human bones recovered from Yarnton, Oxfordshire. Spanning the Neolithic to Saxon periods, it is in many ways a typical site, but is unusual in that a small Middle Iron Age cemetery was discovered.
All of the data presented here lie within the expected isotopic ranges for the European Holocene, although both faunal and animal δ¹⁵N values are higher than is commonly found. The faunal data show the expected pattern for the animals studied (horse, cattle, sheep/goat, pig and dog), with pigs being more omnivorous than ruminants, and dogs eating an isotopically similar diet to humans. The animals' diets had higher δ¹⁵N values during the Roman period as compared to the Iron Age, although it is unclear if this reflects an environmental change or alteration in animal management practices. Despite the site's riverine location, there is no isotopic evidence for fish consumption. No distinctions were found based on gender or burial position within the Iron Age cemetery. Age-based distinctions were found in δ¹³C values, although these cannot be fully explained. As expected, infants have a higher nitrogen isotopic value than other individuals, reflecting the consumption of their mother's milk.     

Calcium isotopes in archaeological bones and their relationship to dairy consumption

The calcium isotope ratios (δ^44/42Ca) of bones from humans and fauna from three archaeological sites, Taforalt, Abu Hureyra, and Danebury, are evaluated in order to assess whether calcium isotope ratios of bones can be used to detect dairy consumption by adult humans. At each site the fauna δ^44/42Ca is the same regardless of species, while the humans have lower δ^44/42Ca than the local animals by 0.24–0.41‰ (site means). However we cannot ascribe this difference to dairy consumption, given this human–faunal difference also occurs in Epipalaeolithic and Mesolithic adult humans, where dairy consumption is unlikely. Rather, this difference appears to be a result of differences in metabolic processes or other aspects of diet between humans and fauna. Minimal isotopic change in sequential acid leaches of bone powders and consideration of the high calcium concentration in bone suggest that bone calcium isotope ratios are not substantially affected by diagenetic change.     

Kana Tamata or Feasts of Men: An Interdisciplinary Approach for Identifying Cannibalism in Prehistoric Fiji

By integrating osteological, taphonomic, archaeological and stable isotopic data, we test for cannibalism in the Lau Group, Fiji and discuss the potential underlying cause(s) and context(s) of this behaviour. First, we compare taphonomic and element representations of human skeletal material from two contexts in Fiji, examining human bone fragments from archaeological sites, including middens and burials in the Lau Island Group. Fourteen sites produced human remains. Only two of those sites included distinct human burial contexts, but in the remaining 12 sites, the human bone was recovered from middens or contexts where midden was mixed with possible secondary burials. A total of 262 number of identified specimens per species, representing an estimated 15 minimum number of individuals make up the Lau human assemblage. Second, we analysed bones contained in 20 individual human burials from four different sites that are housed at the Fiji Museum for comparative purposes. Third, we examine previously published stable isotopic (δ¹³C, δ¹⁵N) analysis of bone collagen to gauge protein consumption of likely cannibalised humans in midden contexts and potential cannibals from primary burials. We model a cannibalistic diet category within the context of isotopically measured Pacific Islands food groups and apply an isotopic mixing model to gauge plausible dietary contributions from six sources including human flesh. Isotopic mixing models of the Lauan samples illustrate a high diversity in reconstructed diets. The percent contribution of human flesh is low for all individual Lauans. We conclude that mortuary rituals evidenced by sharp‐force trauma may suggest non‐nutritive and non‐violent practices that may have included the consumption of small amounts of human flesh. Copyright © 2012 John Wiley & Sons, Ltd.     

Identification of pastoral sites using stable nitrogen and carbon isotopes from bulk sediment samples: a case study in modern and archaeological pastoral settlements in Kenya

The identification of pastoral sites in the East African archaeological record is problematic. Recently, a method for the identification of degraded livestock enclosure sediments had been developed that takes into account the geoarchaeological indicators of micromorphology, phytolith concentrations and the mineral assemblages. This suite of indicators may not always be present in degraded livestock enclosure sediments. This study presents an additional indicator by which degraded livestock enclosure sediments may be identified, namely the isotopic composition of organic nitrogen measured on bulk sediment samples. We studied a highly controlled ethnoarchaeological sequence of abandoned Maasai livestock enclosure sediments sampled in Rombo area, southern Kenya. The results were compared to archaeological sediments from the Elmenteitan Pastoral Neolithic site of Sugenya, southwestern Kenya, radiocarbon dated to ca. 2000 BP (uncalibrated). The sediments from both sites were studied using all four types of analyses, i.e., micromorphology, mineralogy, phytolith concentrations, and stable carbon and nitrogen isotopic compositions on bulk sediment samples. The results show that in abandoned livestock enclosure sediments of known ages a significant enrichment in the heavy nitrogen isotope (¹⁵N) occurs, and that carbon isotopic compositions may be useful for differentiating cattle from caprine enclosures following their dietary preferences (i.e., grazers vs. browsers). A similar pattern of ¹⁵N enrichment is observed in sediments sampled within the site of Sugenya while sediments sampled outside the site's perimeter are generally depleted in ¹⁵N. The micromorphological, mineralogical and phytolith analyses support the conclusion that the sediments from within the site of Sugenya represent degraded livestock enclosure sediments. The carbon isotopic composition from the degraded dung deposits strongly suggests that livestock kept at Sugenya were cattle. Overall, this study presents new empirical data that can be used for the identification of livestock enclosures, and shows that the isotopic signatures and geoarchaeological indicators can preserve for at least two millennia.     

Chemical analyses of organic residues in archaeological pottery from Arbon Bleiche 3, Switzerland – evidence for dairying in the late Neolithic

Fatty acids distribution and stable isotope ratios (bulk δ¹³C, δ¹⁵N and δ¹³C of individual fatty acids) of organic residues from 30 potsherds have been used to get further insights into the diet at the Late Neolithic (3384–3370 BC) site of Arbon Bleiche 3, Switzerland. The results are compared with modern equivalents of animal and vegetable fats, which may have been consumed in a mixed ecology community having agrarian, breeding, shepherd, gathering, hunting, and fishing activities. The used combined chemical and isotopic approach provides valuable information to complement archaeological indirect evidence about the dietary trends obtained from the analysis of faunal and plant remains. The small variations of the δ¹³C and δ¹⁵N values within the range expected for degraded animal and plant tissues, is consistent with the archaeological evidence of animals, whose subsistence was mainly based on C₃ plants. The overall fatty acid composition and the stable carbon isotopic compositions of palmitic, stearic and oleic acids of the organic residues indicate that the studied Arbon Bleiche 3 sherds contain fat residues of plant and animal origin, most likely ruminant (bovine and ovine). In several vessels the presence of milk residues provides direct evidence for dairying during the late Neolithic in central Europe.     

Neolithic Hedgehogs (Erinaceus europaeus) from the Island of Gotland show early contacts with the Swedish mainland

Previous research probing early migrations and contacts in the Baltic Sea area is characterized by the analysis of different chronologies and subsistent strategies on all sides of the Sea. Several studies performed on artifact typology, ceramics, grave rituals and physical anthropology ended with varying results. Although the question of human origins remains inconclusive, in this study, we rely on the phylogeography of an animal associated with humans to elucidate findings regarding prehistoric human migration and contacts.Hedgehogs, along with other fauna on Gotland, were brought over to the island by humans. We examined hedgehog mitochondrial DNA from the Pitted Ware Culture (Middle Neolithic). The genetic signatures of the animals on the island were investigated to determine the animal’s origin.From the 23 bones originally examined, twelve bones from all five locations studied yielded reliable results and resembled published extant Erinaceus europaeus sequences from Sweden, Norway and Denmark. We postulate that a western heritage for the Neolithic hedgehogs on Gotland indicates early human contact with the Swedish mainland.     

Early Copper Age Settlements in the Körös Region of the Great Hungarian Plain

Abstract

This article discusses research carried out by the Körös Regional Archaeological Project from 2000 to 2006 at Early Copper Age Tiszapolgár Culture sites on the Great Hungarian Plain. To build a model of social organization for the period, we incorporated information from regional geomorphological studies, soil chemistry analysis, archaeological surface surveys, remote sensing, and systematic excavations at Early Copper Age sites in the Körös Valley of southeastern Hungary. Previous models characterized the transition from the Neolithic period to the Copper Age as an abrupt shift from a tell-based, sedentary, agricultural lifeway to one based on mobile cattle herding. By studying the transition between these periods on multiple geographic and temporal scales, we have identified a more gradual process with widespread regional variation in cultural patterns. Similar social processes characterize the transition between chronological periods and cultural phases in other parts of the world, and we suggest that a multiscalar approach is effective for building comparative archaeological models of long-term social change.     

Palaeodiet at Eton College Rowing Course, Buckinghamshire: isotopic changes in human diet in the Neolithic, Bronze Age, Iron Age and Roman periods throughout the British Isles

Through isotopic investigations of directly dated human remains recovered from the Eton College Rowing Course, we examine changes in diet from the Neolithic to the Roman period. The human isotope signatures point to a diet based on C₃ terrestrial resources. A significant correlation is visible between human ??¹³C values and time, but no such trend is observed in ??¹⁵N. The animal isotope data from Eton are unevenly distributed, making it difficult to determine if the human values mirror the animal values. To assess whether the results from Eton are typical, we compare our results to isotope data from other British sites dating from the Neolithic to the Roman period. Across this time period, we see a strong correlation between the mean ??¹⁵N of the humans and that of the main domesticated herbivores, with an offset of ??4.5?? between the two. Thus, the changes in the human isotope values are likely linked to changes in the isotopic signatures of the herbivores rather than changes in the protein composition of human diets. By contrast, no clear temporal relationship is observed between the mean ??¹³C of the humans and that of the main domesticated herbivores, with an offset of ??1.4?? between the two. There is, however, a weak correlation observed between the mean ??¹³C of the humans and that of the cattle, which may account for some of the variation in human ??¹³C values between sites. The absence of a strong correlation between mean human and animal ??¹³C suggests that the primary factor influencing human ??¹³C values between sites is dietary composition. The lack of co-variation between ??¹³C and ??¹⁵N is likely to reflect the different representation of dietary macronutrients. Given that the nitrogen results suggest that the animal protein consumption patterns are similar across sites, the human ??¹³C variation between sites is likely to reflect the plant portion of the diet.