Porotic hyperostosis in a Late Upper Palaeolithic skeleton (Villabruna 1, Italy)

Porotic hyperostosis (PH) is a well‐recognised skeletal indicator of physiological stress occurring during the early years of childhood growth. Although frequently found starting from the Neolithic, PH is poorly documented among earlier Palaeolithic hunter–gatherers. This study reports a case of PH in a Late Upper Palaeolithic skeleton (Villabruna 1) from northern Italy. Macroscopic and radiographic examinations of the skeleton show symmetric porotic lesions of the cranial vault, hair‐on‐end appearance, thinning of the cortical bone, diploic expansion and very slight cribra orbitalia (CO). All lesions are highly remodelled and suggest a condition suffered long before death. A differential diagnosis, carried out in order to discriminate between infectious and acquired conditions, points to anaemia as likely aetiology for the changes observed. Absence of postcranial involvement, lesion healing and survival to adulthood suggest a diagnosis of acquired anaemia. Among acquired anaemias, both dietary and infectious models are discussed in light of the individual's skeletal characteristics, as well as geographic location, paleoenvironmental data, subsistence modality and dietary information. The combined analysis of these data suggests that parasitic infestation resulted in megaloblastic anaemia in this individual. Copyright © 2009 John Wiley & Sons, Ltd.     

Thalassemia: macroscopic and radiological study of a case

Differentiation of the genetic and the acquired anaemias, particularly in areas of the world where they may co‐exist, has been a challenge for palaeopathologists for over 100 years. In this paper we present macroscopic and radiographic skeletal lesions that are associated with the thalassemias in a 14‐year‐old girl from a modern reference collection of the University of Athens. This individual is of known sex, age, cause of death, place and dates of birth and death. The case is examined in terms of epidemiology, growth, distribution and severity of lesions and differential diagnosis. The entire skeleton is affected by marrow hyperplasia: lesions of the axial skeleton are extreme, and the appendicular skeleton is severely affected as well. The odontofacial manifestations that are diagnostic of thalassemia and differentiate it from other anaemias are present and include: maxillary and mandibular hyperplasia, reduced sinuses, displacement of maxillary dental structures, overbite, and generalised osteopenia. The development of extreme bone lesions and the ‘advanced’ age‐at‐death of this individual is explained as either the result of thalassemia major under a low transfusion regimen that was the norm during her lifetime, or to a form of thalassemia intermedia that allows survival to later life at the expense of gross skeletal alterations. The present status of skeletal studies in Greece does not support the identification of a genetic anaemia in past populations. The potential contribution of the current analysis in differentiating the anaemias in antiquity is evaluated. Copyright © 2006 John Wiley & Sons, Ltd.     

Multicentric osteosarcoma associated with DISH,in a 19th century burial from England

Osteosarcoma is a rare type of malignant neoplasm that is most frequent in adolescents and young adults although it can develop at any age. It can metastasize from a primary site in bone to other bones and soft tissues. Usually the disorder causes a single bone‐forming lesion (unicentric) but some cases have multicentric, bone‐forming lesions. Some of these lesions develop at different sites at different times. In a second variant of multicentric osteosarcoma, synchronous bone‐forming lesions develop at multiple sites. Distinguishing between these two types of multicentric osteosarcoma is challenging in a clinical context and the criteria for doing so are unlikely to be met in an archaeological burial. Wolverhampton burial HB 39 was excavated from an early‐nineteenth century cemetery site in England. It consists of the incomplete skeleton of an adult male of at least 45 years of age with multicentric osteosarcoma. The individual represented by this burial also had diffuse idiopathic skeletal hyperostosis (DISH). Three of the bone‐forming lesions associated with osteosarcoma developed on the bony outgrowths related to DISH. Copyright © 2010 John Wiley & Sons, Ltd.     

“The Roman Giant”: Overgrowth Syndrome in Skeletal Remains from the Imperial Age

A very tall skeleton was found during archaeological excavations in the territory of Fidenae, an administrative centre of the Roman territorial organization, situated along the Via Salaria about 7 km north of Rome (Italy). The individual was a young male, dated back to the Imperial Age (3^rd century AD), presenting a very tall but normally proportioned stature, estimated around 202 cm. The long bones showed incomplete epiphyseal union; therefore, the stature would probably have been taller, if he had lived longer. In this work, the metric data are compared with those from the Roman Imperial Age population, and differential diagnosis is discussed. The skeletal evidence is characteristic of a form of gigantism, a rare growth disease that may be linked to different syndromes. The most common etiology is associated with a dysfunction of the pituitary gland, which causes overproduction of the growth hormone (hGH) during childhood. This endocrine disorder stimulates cartilaginous activity at the growth plate, delaying epiphyseal fusion and resulting in increased bone length. Copyright © 2013 John Wiley & Sons, Ltd.     

Bone weight: new reference values based on a modern Portuguese identified skeletal collection

Skeletal weight and/or weight of the different bones of the human skeleton are currently used in a wide range of applications such as archaeological cremations and forensics. Still, few reference values are available that compare the mean weights for the different skeletal parts. In this paper we present new reference values for total skeletal weight, including the weight of the different skeletal bones based on a modern Portuguese Identified Skeletal Collection (CEI) curated in the Museum of Anthropology of the University of Coimbra (Portugal). The mean weight of the entire skeleton for the CEI pooled sample is 3323.8 ± 779.6 g. Sex and age differences were investigated. As expected, males display heavier bones, at a statistically significant level. The mean weight of the male skeletons is 3850 g, and 2797.6 g for the female sample. Age differences were found, especially for the female samples in the 29–39 versus 50–59 and/or >60 age groups, probably as a consequence of age‐related bone loss in post‐menopausal women. For the male sample, no clear age‐related trend was observed but for the unexpected result that the second highest bone weight recorded is in the oldest age group (>60 years). This could result from high mechanical loadings and thus greater bone robusticy and amount of cortical bone. The percentage of weight of the different parts of the skeleton was also calculated. These new values can be quite useful in the study of very fragmentary and/or commingled human remains, recovered from historic or forensic contexts, since the complete identification, by side, of the remains is not necessary. Copyright © 2008 John Wiley & Sons, Ltd.     

Humerus Varus in a Subadult Skeleton from the Medieval Graveyard of La Madeleine (Orléans,France)

The skeleton of a subadult individual from the medieval graveyard of La Madeleine (Orléans, France) exhibits varus deformity of its right humerus. The affected bone is shortened, the diaphysis is angulated and the head is grossly deformed. The skeleton is otherwise normal. Several diagnoses are debated, including mucopolysaccharidoses, thalassemia, infection and trauma, the latter being preferred. The severity of the bone changes suggests that the growth disturbance occurred early in the life of the individual. We therefore suppose that the causative trauma occurred at the time of birth or during the early postnatal period. Copyright © 2011 John Wiley & Sons, Ltd.     

A Case of Pituitary Gigantism and Acromegaly in Anatolia (Tasmasor,Erzurum, Turkey)

In 2003, a tall skeleton belonging to a young adult individual was recovered from a post‐medieval cemetery situated north‐east of the modern city of Erzurum, Turkey. The sex of the individual was not possible to determine. The predicted stature values range from 182 to 200 cm with the average 188.94 cm. The growth was proportional on almost all bones, except for the cranial and facial bones. The selected metric data were compared with the mean values of the male individuals from the same cemetery for differential diagnosis. The individual exhibits characteristics of both pituitary gigantism and acromegaly due to the tumour formation developed in the intrasellar area. Pathological features such as severe osteoarthritis, enthesis and new bone formation around and on the joint surface of the post‐cranial bones, kyphoscoliosis and ankylosis are in agreement with these diagnoses as well. Copyright © 2017 John Wiley & Sons, Ltd.     

Aging mountain gazelle (Gazella gazella): refining methods of tooth eruption and wear and bone fusion

This paper refines current methods for aging mountain gazelle (Gazella gazella), one of the most common species hunted by prehistoric humans in the southern Levant. Most importantly, we present a new tooth wear scheme for aging the lower deciduous fourth premolar (dP₄), the lower permanent molars (M₁, M₂, M₃) and the lower fourth premolar (P₄) of the gazelle. Broader wear stages for complete mandibles and individual teeth and bone fusion stages are also provided. Finally, a comparison of the tooth wear and bone fusion data indicates that the two methods can be linked at the important transition when a gazelle reaches adulthood (ca. 18 months of age). The completion of the fusion of the gazelle skeleton corresponds directly to the replacement of the dP₄ by the P₄ and the beginning of wear of the M₃. The coincidence of these aging indicators allows for direct comparison of gazelle bone fusion and tooth wear data.     

Skeletal pathology of a high‐ranking official from Thrace (Turkey,last quarter of the 4th century BC)

A male human skeleton from Thrace dated at around 320 BC was found in a wealthy tomb 12 km from what is now Tekirdağ, Turkey. The occupant of the tomb died at the age of 40–45 years and has a pathologic left humerus caused most probably by traumatic injury. It is 9 cm shorter than the right humerus. A drainage fistula penetrating into the medullary cavity occurs at its proximal end. Due to severe arthritic destruction, the left humeral head has completely lost normal articulation, with the glenoid cavity of the left scapula displaying, in turn, severe erosive lesions and important reduction in its articular surface. A marked abscess is discernible bilaterally on the chondro‐costal sternal end. The individual also has a hip with a total sacroiliac joint fusion and shows slight or moderately developed exostoses on different parts of his skeleton. These joint destructions most likely indicate that he suffered from chronic osteomyelitis and septic arthritis. Copyright © 2005 John Wiley & Sons, Ltd.     

Use of Computed Tomography and Three‐Dimensional Virtual Reconstruction for the Examination of a 16th Century Mummified Dog from a North German Peat Bog

The corpse of a well‐preserved dog was recovered from a peat bog in the region of Burlage, Germany, in 1953. The dog, which dates to the 16th century, retained extensive soft tissue and fur on the postcranial body, although the head is skeletonised. Computed tomography was used to determine the extent of the preservation of the soft tissue, to determine sex and age and to attempt to identify trauma, pathology and potential cause of death. The analysis of the CT data indicated that the dog was an immature or adolescent male. Substantial soft tissue was preserved, including some internal organs. The entire skeleton was present, with the exception of the mandible and parts of the paws; all of the bones were flattened and some were distorted because of demineralisation in the peat. Partial disarticulation of the vertebral column was postmortem, although there was no further evidence of trauma or pathology on the skeleton or soft tissue. The cause of death could not be conclusively determined. To the knowledge of the authors, this dog represents the only known example of a complete historical nonhuman bog body with preserved soft tissue. Copyright © 2011 John Wiley & Sons, Ltd.     

Age Estimation by Tympanic Bone Development in Foetal and Infant Skeletons

This article analyses age‐related changes in the development of the temporal bone in a sample of foetal and infant skeletons to contribute to the estimation of age by means of this skeletal element in bioarchaeological and forensic contexts. The sample was selected from a contemporary documented skeletal collection and is constituted by the temporal bones of individuals of both sexes (n = 37) with ages from 23 weeks of gestation up to 11 postnatal months. Different states of fusion of the tympanic ring and development of the tympanic plate were scored separately, and intra‐observer consistency was evaluated. Both documented chronological age and skeletal estimated age were examined in relation to successive stages of fusion and development in the pre‐ and postnatal periods. It was found that the state of fusion of the tympanic ring is especially useful in the discrimination of foetal and postnatal individuals, whereas the development of the tympanic plate is helpful in the differentiation of individuals from and below 3 postnatal months. Even though a certain degree of overlap between successive stages was observed, the fusion of the tympanic ring and the development of the tympanic plate constitute good indicators of age in foetal and infant skeletons. Copyright © 2015 John Wiley & Sons, Ltd.     

Periostitis and osteolysis in a medieval skeleton from South‐West Hungary: (Leprosy,treponematosis, tuberculosis or hypertrophic osteoarthropathy) A diagnostic challenge!

This paper uses macroscopic and radiological examinations to provide differential diagnoses of pathological lesions in the well‐preserved skeleton of a young male from the medieval site of Zalavár in South‐West Hungary. Macroscopic inspection of the skeleton revealed conspicuous thickening of the tibiae and fibulae with ‘tree bark’ appearance of the cortex. Periosteal proliferations are also found on the calcanea and on the posterior part of the femora. The metatarsals showed bony proliferation and bone dissolution. No alteration of the axial skeleton or the skull was noted. Radiographs showed thickening of the cortex of tubular bones due to a multilayered type of periosteal apposition. The likelihood of these symptoms being the result of melorheostosis, hypervitaminosis A, fluorosis, thyroid acropachy, endosteal hyperostosis, tuberculosis (TB), hypertrophic osteoarthropathy (HOA), treponematosis and leprosy is reviewed. None completely explains the entire spectrum of pathological lesions in the current individual, but the individual may have suffered from two co‐existent diseases. As a clinical entity, the changes in the metatarsals are compatible with leprosy, whereas the periosteal proliferations of the lower limbs point to a diagnosis of HOA as a secondary syndrome. Thus, a combination of leprosy and TB is suggested as a potential diagnosis. Although not definitive, our differential diagnosis was able to exclude a number of conditions producing periosteal apposition. Copyright © 2011 John Wiley & Sons, Ltd.     

Terminal Pleistocene Later Stone Age Human Remains from the Mlambalasi Rock Shelter,Iringa Region,Southern Tanzania

This paper introduces research at the Mlambalasi rock shelter in the Iringa Region of southern Tanzania. The deposits are composed of a historic and Iron Age occupation, a microlithic Holocene Later Stone Age (LSA), and then a macrolithic Late Pleistocene LSA. Middle Stone Age deposits are also present on the slope in front of the rock shelter. Excavations in A.D. 2002, 2006, and 2010 yielded fragmentary human remains as well as pottery, iron, stone tools, faunal bone, and glass and ostrich eggshell beads. Among the human remains, four individuals are present: two adults and a juvenile were found in the same LSA context, and another adult associated with the Iron Age/historic period. The most complete skeleton is an adult of indeterminate sex that was found in situ in an LSA deposit. Charcoal in proximity to the bone was AMS radiocarbon dated to 12,925 cal BC (OxA‐24620), which is consistent with radiocarbon dates on giant land snail shells from above and below the remains. The skeleton exhibits a series of pathological changes such as extensive dental wear and carious lesions, as well as damage most likely caused by termites, post‐mortem. The most striking aspect of this individual is its small size; stature and body mass estimations place it in the range of historic Khoesan from southern Africa. Consequently, this research adds to the discourse regarding the existence of small‐bodied people in the East African LSA. Findings from this new skeletal sample will contribute to studies of human biology and variation in Africa during the terminal Pleistocene and Holocene. Copyright © 2013 John Wiley & Sons, Ltd.     

Estimation of adult skeletal age‐at‐death: statistical assumptions and applications

We examine the statistical assumptions underlying different techniques of estimating the age‐at‐death of a skeleton from one or more age indicators. The preferred method depends on which property of the distribution of the data in the reference sample is preserved in the skeleton to be aged. In cases where the conditional distribution of age given indicator is preserved, we provide ‘look‐up’ tables giving essentially unbiased age estimates and prediction intervals, using a large reference sample and the auricular surface and pubic symphysis age indicators. Where this assumption is violated, but the conditional distribution of indicator given age is preserved, we find that an alternative model which attempts to capture the biological process of development of an individual has some attractive features, which may make it suitable for further study. Copyright © 2006 John Wiley & Sons, Ltd.     

New density data for unfused and fused sheep bones,and a preliminary discussion on the modelling of taphonomic bias in archaeofaunal age profiles

The analysis of mortality profiles forms an important part of zooarchaeological analysis. It has long been assumed that the bones of immature animals are more prone to taphonomic destruction than those of mature animals, potentially distorting mortality profiles. This study measures the DPD (a proxy for taphonomic strength) of the postcranial skeletons of 91 modern sheep of known age. The data produced are used to demonstrate that the DPD of unfused bones is indeed generally lower than that of their fused equivalents, although considerable inter-individual variation exists. It is further suggested that bone density increases throughout the life of an animal and occurs independently of the fusion of its bones. Consequently, taphonomic bias is partly dependant on the age structure and not the fusion status of the deposited assemblage. Relative levels of bias can only be assessed if this age structure is constant for all elements being considered (e.g. if the material were deposited as entire carcasses). Finally, because potential age-related bias is not equal for all elements, the degree of bias associated with a particular fusion group may partly depend on the numbers of certain elements within that group.     

Leprosy in a skeleton from the 7th century necropolis of Vicenne‐Campochiaro (Molise,Italy)

Analysis of the skeleton from tomb 144 of the early medieval necropolis of Vicenne‐Campochiaro in Central Italy revealed several features indicative of leprosy. The skeleton belongs to a male estimated to be between 20 and 25 years of age at death. The distal halves of the 1st and 2nd left metatarsals present acro‐osteolysis and both legs show severe subperiosteal bone reaction. The facial skeleton shows changes compatible with a chronic inflammatory process, possibly due to an infectious disease. The anatomical distribution of the lesions and their association with other skeletal lesions seems to be compatible with a near‐lepromatous form of leprosy. A differential diagnosis is made, and the skeletal traits pathognomonic of leprosy are discussed. Copyright © 2005 John Wiley & Sons, Ltd.     

Osteometric Analysis of South American Sea Lions (Otaria flavescens) Pups From Patagonia. An Assessment of Their Use as Indicators for Seasonality in Archaeological Sites

Zooarchaeological analyses of pinniped remains have been scarce in South America because of lack of specific studies of species determination and estimation of age and sex. Nonetheless, the presence of small and unfused bones (assigned as pup remains) of Otaria flavescens (O. flavescens) in the Patagonian archaeological record has been frequently used as an indicator of summer occupation accomplished by ecological analogues. For this reason, we carried out a postcranial skeletons osteometric study of modern pups of South American sea lions (O. flavescens) with known sex and aged 0–12 months, allowing a more precise age estimation of archaeological samples. Then, we revisited the evidence for seasonality in the Cabo Virgenes 6 site at Cabo Virgenes locality, one of the archaeological localities in Patagonia where the seasonality has been determined only on the basis of the presence of unfused pup bones. Our metric data study on a modern sample shows that the regression analyses generated a complementary and dependable age estimation model for unfused appendicular bones of O. flavescens between 0 and 12 months old that can be applied to samples recovered from archaeological sites. These osteometric studies of postcranial elements allow us to adjust the age estimation of the animal's death and re‐examine seasonality of the Cabo Virgenes 6 site (Santa Cruz, Argentina). Copyright © 2014 John Wiley & Sons, Ltd.     

Postcranial anatomy of the early notoungulate Allalmeia atalaensis from the Eocene of Argentina

We redescribe and interpret the postcranial elements of Allalmeia atalaensis Rusconi, found in the Divisadero Largo Formation, Mendoza, Argentina. Allalmeia is one of the few basal notoungulates with preserved postcranial remains. Rusconi initially documented a skull, several vertebrae and part of the appendicular skeleton but these specimens were subsequently lost. Recently though, distal components of the holotype humeri, the proximal portion of the right femur, several broken diaphyses and most of the left and right pes (MCNAM-PV 507), were relocated. Preparation of this material has revealed new details, especially from the plantar side of the feet. The calcaneum has a distal peroneal process with marked rugosity in the lateral end, a well-developed calcaneal plantar tubercle and a large sustentaculum tali. The naviculars exhibit a well-developed medial tuberosity and slightly contact the calcaneum on the dorsal side, a condition called a ‘reverse alternating tarsus’. The disposition and morphology of the cuneiforms are similar to those of more recent typotheres and the archaic ungulate Tetraclaenodon puercensis. The phalangeal rows are oblique to each other, as in some extant digitigrade mammals. The ungual phalanges are claw-like suggesting that they might have borne claws. Long bones are gracile in comparison with other notoungulates, but with well-developed muscle origins and insertions. The use of body-mass allometric equations and a comparison with extant analogues suggests that Allalmeia was a small, generalized, digitigrade animal. An estimated body mass of approximately 3 kg is consistent with oldfieldthomasiids and archaic ungulates.     

The effect of growth on stable nitrogen isotope ratios in subadult bone collagen

Stable nitrogen isotopes have been used to reconstruct infant feeding practices as nursing infants have elevated δ¹⁵N ratios compared with their mothers. However, infancy is also a time of rapid growth, which may alter nitrogen isotope diet‐to‐tissue spacing. Several studies have documented a decrease in δ¹⁵N during growth in tissues with relatively fast accretion rates. This study investigates the effect that the growth of long bones, via collagen accretion, has on δ¹⁵N ratios. Long bones from individuals aged seven to nineteen years were obtained from a protohistoric ossuary in Ontario, Canada. Analysis of juveniles and adolescents permitted the examination of growth in a group who were not also nursing. It is concluded that a nitrogen isotope growth effect is not detectable in bone collagen from juveniles and adolescents, because: (1) δ¹⁵N ratios are not significantly different among the epiphyses, metaphyses and diaphysis of a growing long bone; (2) δ¹⁵N ratios are not significantly different between faster‐growing versus slower‐growing metaphyses; and (3) δ¹⁵N ratios are not significantly different between bones (or areas of a bone) that are still undergoing growth, versus bones that have ceased growing. The relatively slow speed of collagen accretion may explain why a growth effect is not manifested. Ultimately this research lends support to the use of nitrogen isotopes from bone collagen for infant feeding reconstructions. Copyright © 2009 John Wiley & Sons, Ltd.     

Metastatic carcinoma in a 14th–19th century skeleton from Constância (Portugal)

During 2002, the extensive archaeological excavations of the ancient Constância necropolis (Centre of Portugal), dated from 14th–19th centuries, resulted in the exhumation of 151 individuals. Among the several paleopathological cases, a middle‐aged female skeleton with osteolytic lesions in her skull, axial skeleton, upper limbs and femurs was observed. These pathological findings are characterised by an asymmetric pattern with osteolytic focus of distinct size and irregular shape. Some skeletal elements display both osteolytic and osteoblastic lesions. The latter exhibit deposition of fine layers of woven bone. Lesions were observed macroscopically and radiology was used as a complementary method of scrutiny, especially in cases of unclear observation. The case was diagnosed as that of a probable metastatic carcinoma due to the multifocal distribution of the lesions in areas of intense haematopoietic activity, their morphology and some osteoblastic responses, as well as the presence of pathological fractures in the ribs. The skeleton's sex and age at death are in agreement with the proposed diagnostic, constituting the first case of malignant carcinoma detected in non‐identified Portuguese human skeletal remains. Copyright © 2009 John Wiley & Sons, Ltd.