The geographic origins of Nasca trophy heads using strontium,oxygen, and carbon isotope data

Scholars continue to debate the identity of individuals curated as trophy heads in the Nazca Drainage of southern Peru (c. 1–800 AD). What was the role of trophy heads in Nasca society? Were they victims of warfare or venerated ancestors? Strontium, oxygen, and carbon isotope data from archaeological human tooth enamel from Nasca trophy heads from Aja, Cahuachi, Cantayo, Majoro Chico and Paredones, and from individuals buried in Nasca cemeteries at Cahuachi, Cantayo, and Majoro Chico elucidate the geographic origins and paleodiet of trophy heads in the Nazca Drainage. The ⁸⁷Sr/⁸⁶Sr and δ¹⁸O_c(V-PDB) data from both the trophy heads and the Nazca Drainage burials are all quite variable, and do not support the hypothesis that the Nasca trophy heads were obtained from a geographically-distinct population. Similarly, the δ¹³C_c(V-PDB) data demonstrates that the individuals included in this study consumed similar diets. These data suggest that the Nasca trophy heads likely derive from the local Nasca population. Rather than obtain heads from enemy warriors through geographic expansion or warfare as seen in other parts of the world, this complex social practice existed within the Nasca polity throughout space and time.     

Dietary shifting in the Nasca Region as inferred from the carbon- and nitrogen-isotope compositions of archaeological hair and bone

The topography of the Andean Region results in short travelling distances among ecologically distinct production zones. As such, it is possible to infer relative changes in residence based on the spatial variability in the isotopic composition of food resources. Here, we determine long-term diet through carbon- and nitrogen-isotope analysis of bone collagen for twenty-nine individuals from Cahuachi, an important ceremonial centre in the Nasca Region. We also reconstruct the temporal sequence of change in dietary isotopic compositions along hair samples for seventeen individuals buried at Cahuachi and the neighbouring site of Huaca del Loro.     

Oxygen isotopic evidence of residence and migration in a Greek colonial population on the Black Sea

Oxygen isotope analysis was performed on the enamel of the first and third permanent molars of 60 individuals excavated from the Kalfata-Budjaka necropolis associated with the ancient Greek colony of Apollonia Pontica (5th–3rd centuries BC) on the Black Sea coast of Bulgaria. The objective of this study was to examine the geographic origins and residential histories of these individuals and to compare the isotopic data with archaeological evidence derived from the burials in an attempt to distinguish ‘locals’ from ‘non-locals’. The analysis revealed that 55 of the 60 individuals sampled were either locally born or came from areas outside of Apollonia with isotopically similar δ¹⁸O values. Five individuals were identified as ‘non-local’, and their isotope values suggest that they originated from areas with higher δ¹⁸O_w of precipitation, most likely the Aegean region. Unfortunately, the archaeological evidence associated with these five skeletons provides no clues as to their place of origin.     

The consequences of Wari contact in the Nasca region during the Middle Horizon: archaeological,skeletal, and isotopic evidence

During the Middle Horizon (AD 650–1000), the Wari polity expanded its sphere of control in various regions of the Andes, including Nasca on the south coast of Peru. The nature of the interaction between Wari and local communities varied. This study explores the consequences of Wari contact in the Nasca region at the site of La Tiza, which includes individuals dated to the Early Intermediate, Middle Horizon, and Late Intermediate periods. Using evidence for burial ritual along with demographic, paleopathological and isotopic data, population composition, mobility, diet, and health are investigated. δ¹³C data indicate a relatively stable diet through time at La Tiza with evidence for continued maize consumption, and paleopathological results indicate health problems associated with this type of diet. δ¹⁸O and ⁸⁷Sr/⁸⁶Sr data suggest that most of the individuals buried at La Tiza were local to the area. However, two female adults from the Middle Horizon burials are characterized as nonlocal based on δ¹⁸O and ⁸⁷Sr/⁸⁶Sr values and several individuals overlap with the ⁸⁷Sr/⁸⁶Sr local range for the Wari heartland. In conjunction with the use of new tomb types during this period these results highlight the ideological and populational changes occurring with Wari contact at La Tiza.     

Nitrogen cycling in western India as revealed by nitrogen isotopes,and the historic production of saltpetre

Saltpetre (KNO₃) manufacture was a principal monopoly industry in early Modern India when the United Kingdom governed the social and economic systems. The process represented microbial transformation of excrementitious livestock and human wastes deposited around habitats, resulting in formation of salt efflorescence at an economic scale. Low caste people collected these wastes for extraction and production of rough saltpetre under the administrative control of British India. Stable isotope composition (δ¹⁵N and δ¹⁸O) was used to trace details of the microbial transformation processes, often involving the isotopic fractionation of nitrogen and oxygen. Marked elevation in δ¹⁵N and δ¹⁸O values for nitrates in soils and deposits, relative to those of primary organic nitrogen in diets and water sources, characterizes the unique processes of historic saltpetre manufacture in British India.     

A compositional perspective on the origins of the “Nasca cult” at Cahuachi

We provide a compositional perspective on the origins of the Cahuachi cult in Nasca, south coast of Peru. Instrumental Neutron Activation Analysis of ceramics from the Formative site of La Puntilla demonstrate that a small group of pottery known as Nasca 1 blackwares are compositionally consistent with Early Nasca polychromes originating at Cahuachi. These data suggest that the patterns of pilgrimage and group ceremonies exhibited at Cahuachi during Early Nasca began at least in the proto-Nasca phase of Nasca 1 during the Formative. We use these data to further document the Paracas to Nasca transition, a complex and dynamic period in south coastal prehistory.     

Geochemical fingerprinting of hydraulic fracturing fluids from Qusaiba Hot Shale and formation water from Paleozoic petroleum systems,Saudi Arabia

Provenance studies of produced water are essential to trace flow dynamics and reservoir compartmentalization in petroleum systems and to quantify fluid recovery rates from unconventional fracturing. Produced water from a hydraulically fractured well in the Qusaiba Hot Shale in the Northern Exploration Area, Saudi Arabia, was daily monitored and analyzed for water chemistry, and environmental (δ²H, δ¹³C, δ¹⁸O_H2O, δ¹⁸O_SO4, δ³⁴S_SO4, δ³⁷Cl, ⁸⁷Sr/⁸⁶Sr) and cosmogenic isotopes (³H, ¹⁴C, ³⁶Cl), to differentiate from reference fluids of supply water, fracturing fluids, and formation water from adjacent Paleozoic units. Initially, recovered water is composed of fracturing fluids and subsequently replaced by a homogeneous cut of pristine formation water. Formation water is composed of dominant meteoric water (approximately 84 vol%) and minor fossil evaporated seawater. The young ¹⁴C‐apparent age between 6000 and 6700 years BP and depleted δ¹⁸O/δ²H values for the meteoric component confirm the infiltration of surface water into the Qusaiba Hot Shale interval or adjacent units during the Early Holocene Pluvial Period under cooler and wetter climatic conditions than present, which suggest the presence of a very recent, dynamic hydraulic flow system. ³⁶Cl/Cl ratios between 102 × 10^?15 and 31 × 10^?15 are ambiguous and can be attributed to atmospheric recharge close to the coast, mixing of ³⁶Cl‐enriched Quaternary meteoric recharge with ³⁶Cl‐depleted fossil seawater, and/or hypogene production by U‐Th‐enriched host rock. Produced waters from Qusaiba Hot Shale are within the compositional range of Na‐Cl‐type formation water from Paleozoic reservoir units in northern Saudi Arabia with salinities from 30 000 to 130 000 mg l^?1. As a novel technological approach for exploration wells in Northern Saudi Arabia, multi‐isotopic methods were successfully implemented to quantify flowback volumes from hydraulic fracturing, and to fingerprint pristine formation water or pore water in Paleozoic systems on their provenance, residence time, migration pathways, and secondary alteration processes.     

The stable isotopic composition of historical black powder applicable to a Japanese Tanegashima matchlock

The objective of the present study is to employ stable isotope ratio measurements (δ¹⁵N, δ³⁴S and δ¹³C) of the nitrogen, sulphur and carbon in black powder (a mixture of saltpetre, native sulphur and charcoal) as a means of source identification. A sum total of 20 samples of historical black powder applicable to matchlocks were collected from six locations throughout Japan. This classic type of hand gun prevailed up to the introduction of the modern rifles, with quicker loading and higher efficiency, in the mid‐to‐late 19th century. δ¹³C_{charcoal carbon} values showed the predominant use of C₃ plants as a source material. Except for a few unusual samples with a probable exotic origin, δ¹⁵N_{saltpetre nitrogen} values were largely consistent with those of domestic products using classical biotechnology. The isotopic analysis for native sulphur (δ³⁴S_{native sulphur}) indicates a complicated marketing route.     

Tracing the clay source of Nasca polychrome pottery: results from a preliminary raw material survey

This paper describes the results of the first season of a survey in which clays suitable for the production of Nasca polychrome pottery were collected in an effort to (1) determine the geological variability of clay sources in the Nasca region and (2) match clays compositionally to paste groups from previous provenance analyses. Clays were collected, processed, fired, and analyzed using neutron activation. The results demonstrate that clays in the region were compositionally heterogeneous, and that the clay source for the majority of Nasca polychrome pottery sampled thus far can be traced to lower valleys in the region near the ancient ceremonial center Cahuachi. The results have implications for understanding the nature of polychrome pottery production in the Nasca region, and suggest centralized production near the ceremonial center of the majority of painted ceramics consumed in the Southern Nasca Region.     

Refining oxygen isotope analysis in the Nasca region of Peru: An investigation of water sources and archaeological samples

The development of complex societies, irrigation agriculture and sociopolitical transitions are of interest to researchers working in the Nasca region on the south coast of Peru. Occupied for thousands of years, many questions regarding the circumstances of these changes in the area are being investigated. Oxygen isotope analysis provides a method for exploring residential mobility of past peoples during these transitions. This study presents new δ¹⁸O data from water sources that would have been used by the ancient inhabitants, providing important information regarding the oxygen isotope variability in the region and the necessary baseline data for migration studies in this region. Our results suggest that the isotopic composition of water sources in the Nasca region is not highly variable. In addition, archaeological human tooth enamel samples from the sites of La Tiza and Pajonal Alto are analysed. The δ¹⁸O_c results of the human enamel samples confirm the local nature of the burial population, as suggested by previous strontium isotope analysis (⁸⁷Sr/⁸⁶Sr). Copyright © 2010 John Wiley & Sons, Ltd. This article was published online on 13 January 2010. An error was subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected 15 January 2010.     

Diet and mobility in Middle Archaic Florida: stable isotopic and faunal evidence from the Harris Creek archaeological site (8Vo24), Tick Island

We explore diet and mobility in Middle Archaic Florida using human burials and faunal remains from the Harris Creek archaeological site (8Vo24) on Tick Island. We conducted stable carbon and oxygen isotopic (δ¹³C, δ¹⁸O) analyses of 50 human enamel samples and strontium isotopic (⁸⁷Sr/⁸⁶Sr) analysis of a subset of 10. Most individuals (46/50) subsisted on local freshwater and terrestrial resources, with relatively depleted δ¹³C values (C₃) and δ¹⁸O values matching those of central and north Florida water resources within the St. Johns River valley. Eight of 10 burials have local, within valley ⁸⁷Sr/⁸⁶Sr values. Two human burials yield marine δ¹³C and ⁸⁷Sr/⁸⁶Sr values, which we interpret as evidence for across valley movement from the coast. Another two individuals show depleted δ¹⁸O values, likely obtained from northern rivers. We also identified potential food resources and non-local foods from the Harris Creek faunal assemblage. We found six species with estuarine and marine habitat preferences; two of which, whelk and quahog, indicate that people of Harris Creek traveled to or traded with coastal areas.     

Age‐related variation in isotopic indicators of diet at medieval Kulubnarti,Sudanese Nubia

This study compares trends in dietary composition in two large cemetery populations from the site of Kulubnarti (AD 550–800) in Sudanese Nubia. Bone collagen and bone apatite carbonate were analysed to characterise stable carbon, nitrogen and oxygen isotopes. Previous research on these cemeteries has suggested marked differences in nutritional status and health between the populations. Contrary to expectations, there were no significant relationships between any isotopic indicators related to sex or cemetery of burial, suggesting no isotopically‐measurable differences in diet. However, collagen δ¹³C and δ¹⁵N were significantly related to age, suggesting age‐related differences in protein intake or other factors. Weaning trends are gradual and variable, with the range in δ¹⁵N values exceeding 4‰ among infants/young children (0–3 yrs) and standard deviations exceeding 1‰ in collagen δ¹³C and δ¹⁵N for both infants/young children and subadults (4–17 yrs). This suggests varied weaning strategies among both populations and variable diets prior to adulthood. Also observed was a distinct range of isotopic carbon and nitrogen values among individuals classified as subadults (4–17 yrs), who are depleted in collagen δ¹³C and δ¹⁵N relative to adults. However, both infants/young children and subadults are slightly enriched in δ¹⁸O relative to adults, which suggests the presence of non‐local individuals or age‐related variation in water sources. While most isotopic studies of age‐related dietary trends have focused on reconstructing the weaning process, this study presents findings that indicate tripartite isotopic trends distinguishing infancy, subadulthood and adulthood as separate dietary categories. Broad similarities are evident between the results presented here and those from several earlier studies of smaller populations and to nutritional studies of modern communities. These findings suggest that further research into health disparities at Kulubnarti should focus on non‐dietary causal factors, and more generally, that greater attention should be paid to subadulthood in palaeodiet studies. Copyright © 2006 John Wiley & Sons, Ltd.     

Method-dependent variations in stable isotope results for structural carbonate in bone bioapatite

Stable carbon and oxygen isotope values (δ¹³C, δ¹⁸O) were obtained for structural carbonate in the bioapatite of archaeological bones from Guatemala and Sudan using several common analytical methods. For the Sudan samples, the different methods produced δ¹³C values within ±0.1‰ and δ¹⁸O values within ±0.7‰, on average. The isotopic results for the Guatemala samples were similar in reproducibility to the Sudan samples when obtained using methods that employed lower reaction temperatures and reactions in sealed vessels. However, many Guatemala samples had highly variable and extremely low δ¹⁸O values when reacted at higher temperatures in vessels that remained open to cryogenic traps. The latter arrangement caused reaction products to be removed immediately upon their production. The anomalously low δ¹⁸O values are related to the production of a contaminant gas that causes the m/z 46/44 ratio to be lowered, either by adding to the m/z 44 peak or subtracting from the m/z 46 peak. That said, potential contaminant materials were not detectable in “anomalous” bones using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction, or inductively-coupled plasma atomic emission spectroscopy. However, subtle structural and chemical differences between “normal” and “anomalous” samples were observed, most notably in the FTIR ν₂ CO₃ domain. We suggest that these changes promote volatilization of an oxyphosphorus compound and oxygen isotope fractionation between PO⁻ derived from this compound and CO₂ derived from bone carbonate. Production of the contaminant gas and the related “anomalous” δ¹⁸O values is reversible if the reaction occurs within a sealed vessel for a sufficient period of time, which allows a “back-reaction” to occur.     

Horticulturists and oxygen ecozones in the tropical and subtropical forests of Southeast South America

We analyse the isotopic values (δ¹³C, δ¹⁵N) of the diet of pre-Columbian horticulturalist populations from tropical and subtropical areas of southeastern South America, belonging to the Guarani and Taquara archaeological units. The data indicate different trends in each one (T?=?4.21; P?=?0.0004), showing a mixed diet with maize consumption in the Guarani samples (δ¹³C_co?=??15.5?±?1.8‰; δ¹³C_ap ?10.4?±?0.8‰) and a depleted one in the Taquara ones (δ¹³C_co ?18.2?±?1.7‰; δ¹³C_ap ?11.9?±?0.9‰), with a significant internal dispersion in both populations. The first population has higher nitrogen values (δ¹⁵N 11.1?±?0.6‰) compared to the Taquara samples (δ¹⁵N 9.3?±?1‰), suggesting a more carnivorous diet. The recognition of these pre-Columbian mixed diets involves the identification of maize cultivation on the Atlantic side of the southernmost area of South America (Parana Delta, 34° SL). Through the analysis of δ¹⁸O we have identified two isotopic ecozones, the first along the Paraná River Valley, with an average value of δ¹⁸O ?3.7?±?0.5‰ (CV?=?13.5%; CI?=??3.83 / ?3.16), and the second one, located in the Planalto of southern Brazil (Araucaria Forest), with a mean value of δ¹⁸O ?1.5?±?0.3‰ (CV?=?16.5%; CI?=??1.69 / ?1.29). The isotopic data (δ¹³C, δ¹⁵N and δ¹⁸O) suggest human movements between these two ecozones.     

‘SPROUTING LIKE COCKLE AMONGST THE WHEAT’: THE ST BRICE'S DAY MASSACRE AND THE ISOTOPIC ANALYSIS OF HUMAN BONES FROM ST JOHN'S COLLEGE,OXFORD

The recent discovery in St John's College of a mass burial of mostly young adult males with severe perimortem blade trauma has prompted the suggestion that these may be related to the St Brice's Day Massacre in Oxford on 13th November AD 1002. Three radiocarbon determinations suggest that a date in the tenth century is more likely. We have nevertheless undertaken an isotopic study of the bone collagen (δ¹³C and δ¹⁵N) and dental enamel (δ¹³C, δ¹⁸O and ⁸⁷Sr/⁸⁶Sr) in an attempt to answer the question ‘were these individuals of Danish descent?’ Our conclusion is somewhat ambiguous, but the bone collagen suggests a diet more like other Scandinavian populations than that of local groups, and the enamel isotopes point towards a Scandinavian rather than a lowland English origin. Comparison with Oxford Archaeology's recently excavated Weymouth Ridgeway mass burial suggests, however, that the execution of a captured raiding party is more likely than the slaughter of Oxford inhabitants of Danish descent.     

Anthropogenic saltpetre: dual (oxygen and nitrogen) isotopic constraints to the biogeochemical processes

Saltpetre constitutes one of the major ingredients of gunpowder, which was a driving force during the political changes in late medieval to early modern times in Japan. Two types of model saltpetre mineralization are studied. One represents efflorescent saltpetre formed as the byproduct of indigo dye manufacture, whereas the second is anthropogenic, derived from relict nitre‐beds. Here, oxygen and nitrogen isotopic analysis, as expressed by δ¹⁸O and δ¹⁵N notations, is applied. The calculated δ¹⁸O values for ambient water, responsible for the microbial‐mediated oxidation of ammonium into oxygen in nitrates, tended to have higher ranges than average local meteoric waters of the relevant regions. An overall trend could be seen, showing the apparent positive nitrogen isotopic fractionation during the microbial transformation from initial organic nitrogen into nitrate product. Dual isotopic analysis for industrial nitrates provides a useful tool for investigation of the provenance of historic gunpowder. Present anthropogenic saltpetre involves positive control over temperature–moisture regimes and oxygen fugacity during formation, the process being distinctive from those prevailed in British India and other European countries.     

Oxygen isotope seasonality in a temperate estuarine shell midden: a case study from CA-ALA-17 on the San Francisco Bay,California

Seasonality determination using stable oxygen isotope (δ¹⁸O) analyses in archaeological mollusk shell has been largely limited to aquatic settings where one of the two factors that control shell δ¹⁸O – water δ¹⁸O (or salinity) and temperature – is assumed to be constant. Open coastal marine environments reflect the former situation, and tropical estuaries constitute the latter. In an effort to expand stable isotope seasonality to an ecological setting where neither variable remains constant, we present a model of annual shell δ¹⁸O cycle of aragonite deposition derived from instrumental data on salinity and temperature from San Francisco Bay, California. The predicted range of modeled shell δ¹⁸O is consistent with observed δ¹⁸O values in prehistoric and modern shells when local conditions are considered. Measurements of δ¹⁸O taken at 0 mm and 2 mm from the terminal growth margin were made on 36 archaeological specimens of Macoma nasuta from a late Holocene hunter-gatherer site CA-ALA-17, and season of collection was inferred using the shell δ¹⁸O model. We conclude that shellfish exploitation occurred through the year with the exception of fall, which may indicate scheduling conflicts with acorn harvesting or other seasonally abundant resources elsewhere. The model supports the feasibility of stable isotope seasonality studies in temperate estuaries, provided that instrumental records are available to quantify the relevant water conditions at appropriate spatial and temporal scales.     

Isotopically distinct modern carbonates in abandoned livestock corrals in northern Kenya

We report δ¹⁸O and δ¹³C data from modern carbonate in soils and dung samples from 3 recently abandoned livestock corrals in northern Kenya. Calcium carbonate content is higher within ∼5 cm depth that contains a mixture of dung and surface soils of corrals than in soils below 5 cm depth. We radiocarbon dated carbonates from 0.5 to 40 cm depths in two corrals and one control site. Surface carbonates (0.5 cm) from the two corrals were formed from modern carbon (>1955) when the corrals were active, while all other carbon is >16,000 years (BP) old. Shallow carbonate is also enriched in ¹⁸O (δ¹⁸O up to 3.0‰) and depleted in ¹³C (δ¹³C up to −12.0‰) with respect to carbonate at deeper levels and at two control sites. The δ¹⁸O and δ¹³C of soil carbonates (δ¹⁸O_SC and δ¹³C_SC respectively) in corrals are inversely correlated for depths up to about 15 cm where organic carbon is greater than 0.5%. Below that depth, there is a positive correlation between δ¹⁸O_SC and δ¹³C_SC values, similar to that observed in a control site.     

Iron mineralization and dolomitization in the Paran Fault zone,Israel: implications for low‐temperature basinal fluid processes near the Dead Sea Transform

Petrography, Eh‐pH calculations and the stable isotope composition of oxygen are used to interpret geochemical processes that occurred during iron oxide mineralization and dolomitization along the Menuha Ridge segment of the Paran Fault, southern Israel, adjacent to the Dead Sea Transform (DST). Iron mineralization is strongly localized in the fault zone as ferruginous lenses, whereas Fe dolomitization spreads laterally into the Cenomanian‐Turonian carbonate host rock as stratabound beds. The average oxygen isotope fractionation between syngenetic quartz and iron oxides in the ferruginous lenses gives a temperature of 50 ± 10°C and δ¹⁸O SMOW water = ?3.5‰; consistent with an origin from metalliferous groundwater flow in the sedimentary basin. Ferroan dolomite initially formed under strongly reducing conditions, but this was followed by oxidation and pseudomorphic replacement of the dolomite by a mesh of fine‐grained iron oxides (simple zoned dolomites). This cycle of ferroan dolomite formation and replacement by iron oxides was repeated in complex zoned dolomites. Dolomite oxygen isotope compositions fall into two groups: a high δ¹⁸O group corresponding to the simple zoned dolomites and non‐ferroan dolomites and a low δ¹⁸O group corresponding to the complex zoned dolomites. Water‐rock calculations suggest that the epignetic dolomites formed under fluid‐buffered conditions: the high δ¹⁸O group are indicated to have formed at temperatures of ca. 25°C for waters with δ¹⁸O = ?4 to 0‰; the low δ¹⁸O complex zoned dolomites at 50–75°C for waters with the same isotopic composition. A kinetic calculation for a complex zoned dolomite‐bearing bed indicates that dolomitization must have occurred at high values of the dolomite saturation index. This requirement for high Mg supersaturation and the indication that epigenetic dolomitization is more protracted in stratigraphically deeper formations located closer to the DST is consistent with models proposing that Mg‐rich solutions originated in the Dead Sea Rift.