Sagittal Clefting of the Fifth Lumbar Vertebra of a Young Adult Female from Apollonia Pontica,Bulgaria

A rare congenital anomaly in the form of sagittal clefting of the fifth lumbar vertebra was observed in the skeleton of a young adult female excavated from the Greek colonial site of Apollonia Pontica (5th to 3rd centuries BC) on the Bulgarian Black Sea coast. No compensatory changes had occurred to the adjacent vertebrae, and no other skeletal anomalies or pathological conditions were observed in the skeleton. This anomaly, the first of its kind documented in ancient Greek remains, was likely asymptomatic and of no clinical significance. Copyright © 2012 John Wiley & Sons, Ltd.     

Molecular history of tuberculosis from ancient mummies and skeletons

The origin and evolution of the infectious disease tuberculosis (TB) and its pathogens is still not fully understood. An important effort for a better understanding of the underlying mechanisms of TB evolution lies within the investigation of skeletal and mummified material dating back several thousand of years. In this work, molecular data from mummified and skeletal material from different time periods of the Old World are compared, and the current status of ancient mycobacterial DNA analysis in ancient human remains is discussed, with particular reference to the genetic evolution of human TB. The molecular analysis of material from southern Germany (1400–1800 AD), Hungary (600–1700 AD) and Egypt (3500–500 BC) revealed high frequencies of TB in all time periods. In several individuals from ancient Egypt the mycobacterial DNA could be further characterised by spoligotyping. Thereby, evidence for ancestral M. tuberculosis strains was found in the pre‐ to early dynastic material from Abydos (3500–2650 BC), while typical M. africanum signatures were detected in the Middle Kingdom tomb in Thebes‐West (2050–1650 BC). Samples from the New Kingdom to Late Period tombs (1500–500 BC) were characterised as modern M. tuberculosis strains. In concordance with other studies on ancient skeletal and mummified samples, no evidence for the presence of M. bovis was found. These results contradict the theory that M. tuberculosis evolved from M. bovis during domestication, but supports the new scenario that M. tuberculosis probably derived from an ancestral progenitor strain. Copyright © 2007 John Wiley & Sons, Ltd.     

Repeat silica extraction: a simple technique for the removal of PCR inhibitors from DNA extracts

Polymerase chain reaction (PCR) inhibitors are often co-extracted with ancient DNA (aDNA) and when present make the analysis of aDNA difficult, if not impossible. In this study we review previous research on PCR inhibitors and techniques that address their co-extraction with DNA from sub-optimal samples. Additionally, we introduce a simple extraction technique, “repeat silica extraction,” that effectively removed PCR inhibitors from extracts of 7000–8000-year-old human skeletal remains from the Windover archaeological site in Florida and 700–2000-year-old human coprolites excavated from Fish Slough Cave in southern California. A series of tests on these same samples demonstrates that N-phenacylthiazolium bromide is largely ineffective, despite previously reported success using this compound as part of the DNA extraction process. We also describe a method for demonstrating the presence as well as successful removal of PCR inhibitors by use of a “positive aDNA control,” a test necessary to conclude that negative PCR amplification is the result of the absence of preserved DNA.     

Non-invasive Archaeology of Skeletal Material by CT Scanning and Three-dimensional Reconstruction

The remains of an ancient Sumerian skeleton, approximately 7000 years old, were investigated using the techniques of stereolithography. The very fragile and delicate skeletal material was recovered in a block, thus retaining it in the soil matrix. The excavated block was CT-scanned and the skeletal material was rendered in three dimensions. This formed the basis for a stereolithographic model of the mandible, which was used for physical and dental anthropological studies. Skeletal remains may thus be made available for research without having to remove them from the local matrix, which may be an advantage in an archaeological or palaeoanthropological setting. © 1997 by John Wiley & Sons, Ltd.     

Infectious diseases and eating habits at Herculaneum (1st century AD,southern Italy)

This paper shows that many food remains excavated from Herculaneum were microbiologically contaminated, and that the Romans probably had continuous exposure to gastrointestinal diseases. However, palaeopathological analysis of skeletal remains from Herculaneum shows a low prevalence of non‐specific bone inflammation. Pomegranates and figs, consumed by the population, were mainly dried and invariably contaminated by Streptomyces, a bacterium that produces natural tetracycline, an antibiotic. Histological analysis of the human remains demonstrates fluorescence typical of this substance. The tetracycline‐labelled human remains show that the Roman inhabitants of Herculaneum ate food contaminated by Streptomyces, and this may explain the rarity of inflammatory bone diseases at the site due to non‐specific infection in the living population. This interpretation fits with therapeutic indications suggested by Roman physicians that preserved fruits were used to cure some inflammatory diseases. Copyright © 2007 John Wiley & Sons, Ltd.     

A further consideration of neolithic dogs with special reference to a skeleton from Grime's Graves (Norfolk), England

The complete skull and skeleton of a dog were recently excavated from the neolithic flint mines at Grime's Graves in Norfolk. As very few complete skeletons of dogs have been found from neolithic contexts in Britain a detailed metrical comparison was made between the Grime's Graves specimen and dog skeletons of comparable antiquity and completeness from Easton Down and Windmill Hill in Wiltshire.Implicit in the study of the dog from Grime's Graves was the need to date it precisely. As the skeleton could not be sacrificed for radiocarbon dating, closely associated antlers of Red deer (Cervus elaphus) were used for this purpose. The date of the Grime's Graves dog is compared with the dates for other dog remains from recently excavated neolithic sites in the British Isles.     

Weapon injuries in the 12th century Crusader garrison of Vadum Iacob Castle,Galilee

This project analyses the wounds sustained by those defending the Crusader castle of Vadum Iacob, which is to the north of the Sea of Galilee in Israel. To our knowledge this is the first Crusader castle garrison to be excavated and studied, and consequently gives unique information concerning medieval battle wounds. The Muslim forces of Saladin stormed the castle in August 1179. The skeletal remains of five of the garrison who were killed during the siege and the execution which followed have been studied, providing a vivid portrait of what it must have been like in the last hours as the castle surrendered. Multiple sword and arrow wounds were noted, and arrowheads were still in situ at the time of their deaths. All the soldiers appear to have been stripped of their armour and then dumped together with corpses of horses that died in the battle. We explore the nature and anatomical location of the wounds in the context of medieval Islamic weapons, battlefield tactics and the defensive armour of the Crusaders. Despite the limited number of soldiers discovered, the unique nature of this site makes the findings of great significance. Copyright © 2006 John Wiley & Sons, Ltd.     

Earliest evidence of spinal tuberculosis from the Aneolithic Yayoi period in Japan

Tuberculosis has existed from early prehistoric days to modern times. The main causative agents of tuberculosis worldwide are Mycobacterium tuberculosis (M. tuberculosis) and M. bovis, along with M. africanum, M. cenettii and M. microti; these species make up the ‘M. tuberculosis complex’. This worldwide infection has been of special interest to palaeopathologists due to its characteristic bone lesions as well as its great antiquity. Historically, tuberculosis has been recognised in Japan for more than a thousand years. However, the origin and early prevalence of tuberculosis remain unknown. In the present study, we present the earliest evidence of skeletal tuberculosis found in the Aneolithic Yayoi period in Japan (ca. 300 BC to AD 300). The skeletal remains showing typical pathological changes of spinal tuberculosis were dated to between 454 BC and AD 124 by dendrochronological methods using coburied arrow-shield board and house columns made of Japanese cedar. We discuss the early prevalence of this infectious disease and its influence on the population history of the Japanese from prehistoric to Aneolithic times. Copyright © 2006 John Wiley & Sons, Ltd.     

Wild or domesticated: DNA analysis of ancient water buffalo remains from north China

Recent zooarchaeological studies on water buffalo (Bubalus sp.) remains from China and south Asia question the traditional view that water buffalo were first domesticated in Neolithic China over 7000 years ago. The results from several recent population genetic studies of modern domesticated buffalo (Bubalus bubalis) are not consistent with each other, placing the original center of buffalo's domestication in south Asia, southeast Asia, or China. This paper reports a study using an ancient DNA approach to analyze water buffalo remains from Neolithic sites in north China to investigate their affinities with modern domesticated water buffalo, and to shed light on the origin of modern domesticated water buffalo in China.A 169 bp fragment of D-loop mitochondrial DNA was successfully amplified and verified for 13 of 24 bone samples obtained from seven archaeological sites along the Wei River valley in Shaanxi Province, China. The bone samples which yielded positive DNA can be dated to 8000–3600 cal. BP. The phylogenetic analysis of the obtained DNA sequences along with modern water buffalo sequences indicated that the ancient water buffalos were not the direct ancestor of modern domesticated water buffalo. However, the phylogenetic analysis, along with BLAST searches of these ancient DNA sequences, did demonstrate their relatedness to water buffalo more so than to any other bovid species, confirming the existence of indigenous wild (but now extinct) water buffalo species (B. mephistopheles) in ancient China.The DNA analysis of these ancient remains failed to establish direct links between modern domesticated water buffalo (B. bubalis) and indigenous water buffalo (B. mephistopheles) from ancient China. If further DNA studies of more ancient remains from other regions of China confirm the observation of solely indigenous water buffalo species in ancient China, it would suggest modern water buffalo might not have been first domesticated in China.     

Celtic Ritual Activity at Gordion,Turkey: Evidence from Mortuary Contexts and Skeletal Analysis

Primary support for a Celtic presence in Turkey during ancient times comes from textual sources. However, the analysis of human skeletal remains and mortuary practices at the site of Gordion, combined with archaeological findings, provide persuasive evidence of a Celtic settlement including ritual activity. Data are drawn from 47 individuals excavated from the Lower Town area of the site: 21 Later Hellenistic (late 3^rd to 2^nd centuries BCE) and 26 Roman (1^st to 2^nd centuries CE). The two sub‐samples have markedly different paleodemographic profiles. Composition of the Later Hellenistic group is unusual, with very few infants (5%) and primarily young or middle aged adults (52%), whereas the Roman sample has many infant burials (27%) and less than half young or middle aged adults (35%). Burial contexts for the two groups are also distinct, with only one formal interment associated with the Later Hellenistic, the remaining individuals being in mixed groupings of human and animal bones or disarticulated and commingled human skeletal deposits. By comparison, the Roman sample comprises exclusively primary burials, two cremations, and 24 inhumations. Evidence of inter‐personal violence, such as perimortem cranial trauma and decapitation, is totally absent in the Roman group but present in 25% of the Later Hellenistic specimens. The nature of the Later Hellenistic skeletal assemblages and the ritual space in which they were found show similarities to European Celtic remains identified as resulting from ritual sacrifice. The data presented here represent the first comprehensive bioarchaeological approach to these population groups from central Turkey. Copyright © 2012 John Wiley & Sons, Ltd.     

Weapon‐related Cranial Lesions from Medieval and Renaissance Turin,Italy

Archaeological excavations carried out in the square around the Cathedral of S. Giovanni in Turin brought to light burials referable to the medieval and Renaissance periods. The anthropological examination of the skeletal remains allowed to identify two skeletons from the medieval period (10th–11th centuries) and four skeletons from the Renaissance age (15th century) showing weapon‐related cranial injuries. These peri mortem lesions are indicators of interpersonal aggression and in particular of armed conflicts. The two individuals from the early medieval period presented three traumas consisting in sharp force lesions caused by bladed weapons. As regards the Renaissance sample, the majority of the nine peri mortem injuries were sharp force wounds, followed by a blunt force trauma. These distribution patterns might reflect different fighting techniques, whereas the side distribution and location of the skull trauma provide further indications on the fighting modalities. Identification of the weapons that caused these traumas is suggested. The lack of post‐cranial wounds at Piazza S. Giovanni might be explained by the greater attention paid to the head, which was the main target of attack, or by adequate protection of the body through medieval and Renaissance armours. Otherwise, the wounds in the body would have been found only in the soft tissues, with no involvement of the bones. Despite the presence of weapon injuries, the results obtained from the study of the Renaissance sample are different from the findings of other contemporary battlefields. It is highly likely that the individuals of the Renaissance age were not young soldiers employed in war episodes and brought back for burial in Turin after battles that had taken place elsewhere. Instead, they were probably individuals who had died in riots or in other violent city episodes, as the historical records for the Renaissance age seem to confirm. Copyright © 2013 John Wiley & Sons, Ltd.     

Macro- and micro-archaeobotanical study of a vessel content from a Late Neolithic structured deposition from southeastern Hungary

A well-preserved assemblage of pottery and a broken stone artefact were excavated within a long house in the southern part of Tiszasziget, near the town of Szeged, Hungary. The settlement had been inhabited by the Late Neolithic Tisza culture around 5000–4500 BC. Based on the position of the finds it is suggested that the objects represent an ideologically-charged structured deposition. A piece of organic residue found in a mug (No. 18) with three-fold articulation has been subjected to macro- and microfloral analysis. After precise sampling of the residue, the standard methods to recover organic and inorganic plant remains were utilised. Based on the macrofloral and amino acid content analysis, it is suggested that the organic remains were pieces of fermented pastry made of cereal flour. The recovered silicified tissues and articulated phytoliths were subjected to morphometric measurements, which revealed that the food remain placed in the structured deposition was prepared of Triticum and – probably wild – Avena species. Starch granules of cereals were also detected, whilst the palynological evidence supports morphologies most likely to be related to the accompanying weed flora. The fortunate survival of the organic matter and the complex macro- and micro-archaeobotanical approach provided an unique opportunity to gain a better insight to the food preparation of Late Neolithic communities of the Carpathian Basin.     

Radiocarbon Marine Reservoir Effect in Human Remains from the Kitakogane Site,Hokkaido, Japan

Accelerator mass spectrometry (AMS) was used to measure radiocarbon (¹⁴C) in bones excavated from the Late Jomon shell midden at the Kitakogane site, Hokkaido, Japan. Comparison between ¹⁴C ages of terrestrial and marine mammals from the same site showed systematic age differences which could be attributed to the ¹⁴C marine reservoir effect in the western North Pacific Ocean. Furthermore, this effect was clearly observed in human remains from this site, indicating a heavy dependence on seafood. Dietary habit was estimated from δ¹³C and δ¹⁵N measured in the same human remains. Apparent ¹⁴C ages indicated 79% of protein originated from marine sources.     

Metastatic carcinoma in a 14th–19th century skeleton from Constância (Portugal)

During 2002, the extensive archaeological excavations of the ancient Constância necropolis (Centre of Portugal), dated from 14th–19th centuries, resulted in the exhumation of 151 individuals. Among the several paleopathological cases, a middle‐aged female skeleton with osteolytic lesions in her skull, axial skeleton, upper limbs and femurs was observed. These pathological findings are characterised by an asymmetric pattern with osteolytic focus of distinct size and irregular shape. Some skeletal elements display both osteolytic and osteoblastic lesions. The latter exhibit deposition of fine layers of woven bone. Lesions were observed macroscopically and radiology was used as a complementary method of scrutiny, especially in cases of unclear observation. The case was diagnosed as that of a probable metastatic carcinoma due to the multifocal distribution of the lesions in areas of intense haematopoietic activity, their morphology and some osteoblastic responses, as well as the presence of pathological fractures in the ribs. The skeleton's sex and age at death are in agreement with the proposed diagnostic, constituting the first case of malignant carcinoma detected in non‐identified Portuguese human skeletal remains. Copyright © 2009 John Wiley & Sons, Ltd.     

Ancient DNA analysis of desiccated wheat grains excavated from a Bronze Age cemetery in Xinjiang

Wheat has been one of the most important crop in Eurasia since the Neolithic period. Understanding the spread of wheat cultivation is crucial to understanding the spread of agriculture as a whole and the interactions between prehistoric populations across the Eurasian continent. However, the routes by which wheat cultivation spread eastwards have been poorly understood to date, due to the scarcity of plant remains recovered from archaeological sites. Desiccated wheat grains excavated from the Xiaohe cemetery in Xinjiang, and dated to the early Bronze Age, show excellent DNA preservation. Here we present an ancient DNA (aDNA) analysis of wheat (Triticum sp.) grains excavated from Xiaohe and provide the first definitive evidence for bread wheat in China during the Bronze Age. The nuclear ribosomal DNA internal transcribed spacer regions (ITS1 and ITS2) and the intergenic spacer region (IGS) were amplified. The IGS region within the D genome of wheat has a 71 bp insertion that is absent from corresponding regions in the A and B genomes. The results showed that the Xiaohe wheat showed most sequence similarity to hexaploid bread wheat (Triticum aestivum), including the characteristic insertion into the D genome. The presence of bread wheat at the Xiaohe cemetery is discussed in relation to it having spread into Xinjiang by the Bronze Age, providing new insight into the origins of bread wheat in East Asia.     

Dental root dentine translucency used in age determination of medieval Norwegians from Trondheim

A pilot study on eight skulls from the churchyard of St Olav's church in Trondheim, excavated in 1988–1989, was carried out to compare age determined by root dentine translucency with the age assessed by a physical anthropologist. The difference in mean age between the two methods was not significant (p = 0.06). Age determinations based on root dentine translucency were then performed on removable teeth from the adults among the 248 skeletons found in the same churchyard, excavated in 1984–1985. A comparison between the ages determined using root dentine translucency and the ages previously determined by the direct inspection method was performed. A significant systematic difference was seen between the two methods (p = 0.000). The root dentine translucency method gave an age range for the cohort excavated in 1984–1985 between 30 and 72 years, whereas the direct inspection method gave a range between 15 and 63 years. Because evaluation of many criteria are required to achieve reasonably accurate estimates of age in connection with the direct inspection method, expertise in skeletal biology is necessary. The method of measuring root dentine translucency as a tool in archaeological and anthropological work and fieldwork and in forensic science seems to have advantages because the method can be used without previous extensive training.     

Prehistoric wooden bows and arrows in the Turpan Basin,Northwest China: Wood selection and utilization in a mosaic landscape

In the present study, we analyzed seven wooden bows and 13 arrows excavated from the cemeteries of Subeixi Culture (13th century BCE–AD second century) in the Turpan Basin, northwest China. A total of six taxa, viz. Morus sp., Salix sp., Picea sp., Lonicera sp., Betula sp., and Tamarix sp. were identified. Some of these taxa have been generally accepted to be suitable for bow-and-arrow production. All these taxa were local in origin but distributed in different ecotonal zones, including oases, saline-alkali lands, and mountain areas. Previous archaeological studies have confirmed that the Subeixi populations lived an agro-pastoral life. By analyzing the possible provenance of these wood samples, it can be hypothesized that these ancient agro-pastoralists moved between the Turpan Basin and the Tianshan Mountains on a seasonal basis.     

Childhood Cranial Trauma from a Late Roman and Merovingian Context from Michelet,Lisieux, France

The Michelet necropolis in Lisieux, France, dating to the late Roman and Merovingian period, comprises of a large number of well‐preserved subadult remains offering a unique opportunity to better understand childhood trauma in the past. The focus of this study was to determine the amount, type and mechanisms of trauma evidenced in subadults from the 4th–8th century AD, and explore potential circumstances surrounding the trauma. The remains of 109 subadults from the Michelet necropolis were examined for the presence of cranial and post‐cranial trauma. Three individuals exhibited perimortem trauma, one individual had an antemortem cranial injury, and no cases of post‐cranial trauma were identified. Cranial trauma affected 4.1% of children with observable cranial remains (N  = 4/97). The children affected were young (2–7 years old), making it unlikely that they would have participated in militaristic activities. Based on the location, morphology and mechanism of injury identified, it is likely that the perimortem injuries sustained by three children were not accidental. The presence of a number of cranial injuries from this site may be related to increased stress in the community related to the decline of the Roman Empire in Gaul, possible raiding barbarian groups during the 4th–5th centuries, or stresses related to the Gallic aristocracy solidifying political powers in northern Gaul during the 5th–7th centuries AD. The consideration and inclusion of childhood trauma in bioarchaeological analyses allows for a more detailed and in‐depth understanding of violence and childhood in the past. Copyright © 2017 John Wiley & Sons, Ltd.