Dendrochronological and radiocarbon dating of subfossil wood from the Morava River basin

The paper deals with dendrochronological and radiocarbon dating of subfossil trunks found in the basin of the Morava River. The research into subfossil trunks had been conducted in the Czech Republic in the past but the research stopped in 2001. 160 records of measurements of subfossil, predominantly oak trunk samples, are preserved from that time. Three years ago the research was reopened and again the most trunks were found in the basin of the Morava River. 92 samples of oak trunks and 7 samples of other tree species (poplar, elm, maple, beech) were taken from selected sites. The samples were processed in compliance with the standard dendrochronological methodology. To date the samples, Czech, Austrian, Polish and German standard chronologies were used. In cases when the dendrochronological dating was unsuccessful, the samples were sent for radiocarbon dating. In total, 35 samples were dated, mainly by the radiocarbon method. Subfossil trunks from gravel pit Tovačov were dated and classified into 4 different periods, the oldest being from 2780–2190 BC. In Osek nad Bečvou, the samples were taken from two sites. Whereas from the 4 trunks taken in the river channel each was from a different period, the trunks from the gravel pit come from 981–1015 AD. The trunks found in the Morava River basin near Strážnice were dated to the 10^th–15^th century. In the future, we expect to extend the amount of the acquired samples with the aim to create a link to the existing standard chronology or to create a long floating average treering curve.     

Stable carbon isotope analysis of human tooth enamel from the Bronze Age cemetery of Ya'amoun in Northern Jordan

Seventy-five teeth from the Bronze Age cemetery of Ya'amoun were sampled for carbon isotope analysis. The sampled teeth represented the transitional period of the Middle/Late Bronze Age. The results showed that the components of the diet that are reflected in the carbon isotope data remained the same during this period with an average δ¹³C of about −11.87‰. This value reflects more C₃ plant consumption.     

Charcoal from a prehistoric copper mine in the Austrian Alps: dendrochronological and dendrological data,demand for wood and forest utilisation

During prehistory fire-setting was the most appropriate technique for exploiting ore deposits. Charcoal fragments found in the course of archaeological excavations in a small mine called Mauk E in the area of Schwaz/Brixlegg (Tyrol, Austria) are argued to be evidence for the use of this technology. Dendrochronological analyses of the charcoal samples yielded calendar dates for the mining activities showing that the exploitation of the Mauk E mine lasted approximately one decade in the late 8th century BC. Dendrological studies show that the miners utilised stem wood of spruce and fir from forests with high stand density for fire-setting and that the exploitation of the Mauk E mine had only a limited impact on the local forests.     

Stable carbon and nitrogen isotope variations in tooth dentine serial sections from Wharram Percy

Here we report δ¹³C and δ¹⁵N measurements of serial sections of human deciduous and permanent tooth dentine from archaeological samples taken from the medieval village site of Wharram Percy, Yorkshire, UK. We found a pattern of enrichment, for both δ¹³C and δ¹⁵N, where the tooth crown was greater than the cervical part of the root, which in turn was greater than the apical portion of the root and the associated rib collagen values. This pattern reflects a decrease in the consumption of isotopically enriched breast milk and the introduction of less enriched weaning foods in the diet. The (mean±SD) difference between the deciduous second molar crowns and corresponding rib samples from the same individuals after 2 years of age was 1.2±0.4‰ for δ¹³C and 3.2±0.8‰ for δ¹⁵N. The δ¹⁵N values are as predicted, but as there were no C₄plants at Wharram Percy, this 1.2‰ enrichment in δ¹³C represents clear evidence of a carbon trophic level effect in collagen from breastfeeding infants. Carbon and nitrogen results also show that the infant diet among those who died in infancy did not differ from those who survived into childhood. This study demonstrates the promise of using dentine serial sections to study the temporal relationships of breastfeeding, weaning, and dietary patterns of single individuals.     

Paleodietary implications from stable carbon isotope analysis of experimental cooking residues

The regional timing of maize introduction in eastern North America is a long-standing topic of archaeological interest. Most recently, Morton and Schwarcz [2004. Paleodietary implications from stable isotopic analysis of residues on prehistoric Ontario ceramics. Journal of Archaeological Science 31, 503–517] investigated the timing of maize introduction in Ontario through isotope analysis of charred cooking residues adhering to the interior of prehistoric ceramic containers. They interpret their results to suggest maize was incorporated into diets after A.D. 600. We assess their approach and conclusions with stable carbon isotope assays on three sets of experimental cooking residues, evaluating the variable combustion of carbon fractions, contributions of fats and carbohydrates, and the contribution of total carbon. We also undertake multiple resource modeling of two part food mixes with green maize and maize flour. Our results suggest that systematic under representation of maize can result depending on residue composition and that some prior knowledge of C3 plant and animal contents is necessary to interpret stable carbon isotope values on cooking residues. We question the independent use of stable carbon isotope analysis of charred cooking residues as a viable technique for extracting paleodietary information.     

Stable carbon and nitrogen isotope analysis on human remains from the Early Mesolithic site of La Vergne (Charente-Maritime,France)

We report here the results of stable carbon and nitrogen isotope analysis of human and faunal remains from La Vergne (Charente-Maritime, western France), a rare Early Mesolithic burial site (ca. 8500–8000 cal BC). The results for nine humans (average δ¹³C = −19.3‰; δ¹⁵N = 9.4‰) indicate a strongly terrestrial diet, dominated by animal protein, with the possibility of, at best, a slight contribution of marine-derived protein. Given lower sea-levels in the early Holocene, the site would have been some 60–80 km from the sea at the time of its use; nevertheless, contacts with the coast are shown by the presence of numerous marine shell beads in the graves. In the light of the stable isotope results, it is suggested here that such contacts most likely took the form of exchange with coastal communities whose remains now lie underwater.     

Stable isotope analysis of a medieval skeletal sample indicative of systemic disease from Sigtuna Sweden

In Sigtuna, Sweden, several medieval cemeteries have been excavated, from which approximately 800 skeletons have been excavated and analysed. Archaeological finds and anthropological analyses have exposed social differences between the cemeteries. Stable isotope analyses have shown that the inhabitants of the town consumed a mixed diet. Significant differences in dietary patterns between the cemeteries may be related to social stratification.In the outskirts of a churchyard excavated in 2006, bone changes showing systemic inflammatory disease indicative of leprosy were observed in six individuals. The burial location suggests that the affected belonged to a lower social stratum. Bone samples were taken from these six individuals, 19 other human skeletons and five animals from the same cemetery for analysis of the stable isotope composition of carbon (C), nitrogen (N) and sulphur (S).The results showed no significant differences in δ¹³C and δ¹⁵N values between the groups, i.e. the seemingly healthy humans and the humans affected by severe inflammatory disease appear to have had similar diets. Nor was a significant difference observed in δ³⁴S data between the six affected individuals and the rest of the sample, implying that no difference in origins could be observed between the two groups studied. However, a comparison between the present study and the previous analysis resulted in significant differences in carbon values.Based on the results obtained in this investigation it is suggested that if a dietary difference existed between people in the outskirts of a cemetery (for example those suffering from leprosy) and people buried in higher ranked regions, it was not a difference in food source but rather in other parameters. Instead dietary differences and possibly social variations are demonstrated between cemeteries. The results from the present study highlight the hierarchical arrangements of social classes in the early medieval society.     

Stable isotope analysis of the Inca mummy from Nevado de Chuscha (Salta,Argentina)*

This paper presents the carbon (¹³C/¹²C), nitrogen (¹⁵N/¹⁴N), oxygen (¹⁸O/¹⁶O), hydrogen (²H/¹H) and sulfur (³⁴S/³²S) stable isotope values measured in the hair of a female individual from north-western Argentina. The analysis of segments of this tissue allows for the recording of the diet and migratory changes with a short time resolution. The sample is from a mummified young female individual discovered in Chuscha mount, Salta province. It was found at more than 5000 masl, in a mountain sanctuary of the Inca expansion (capacochas). The paper discusses the paleodiet and mobility patterns of this individual in the period before her death, focusing on the isotopic variations in a limited time scale. The results indicate that the individual moved from a different region to the place where she was sacrificed. Furthermore, in the last year the individual was alive, a shift in the isotopic composition of the food consumed is detected: a variation in the importance of C₄ over C₃ resources is evident. The results are compared with the isotopic estimations for other children and young people recovered in archaeological contexts associated with capacochas to infer variability in the geographical trajectories covered during their last months of life.     

Stable carbon and oxygen isotopic compositions of wood ash: an experimental study with archaeological implications

Wood ash, composed mainly of the mineral calcite, is an important component in many archaeological sites. Identification of wood ash in the archaeological record is often difficult due to mixing of ash with other calcitic components of geogenic origin and/or due to diagenetic changes. A recent empirical study using the stable isotope compositions of carbon (δ¹³C) and oxygen (δ¹⁸O) in wood ash enabled the identification of mixtures of wood ash with geogenic calcite and to follow diagenetic changes due to partial dissolution and re-precipitation of ash in two prehistoric cave sites in Israel. Little however is known about the processes responsible for the isotopic compositions of wood ash in relation to formation at various temperatures and the influence on isotopic composition of ash from a variety of plant species. Here we present an experimental study of wood ash formed by burning three C3 tree species and one C4 desert bush at different temperatures. The results indicate that there are significant differences in the isotopic compositions of carbon and oxygen between wood ash that forms by combustion at a relatively low temperature (500 °C) and at a higher temperature (900 °C). In addition, we show that the isotopic composition of carbon and oxygen in high temperature wood ash approaches equilibrium over a period of several months and that the carbon isotopic composition of low temperature wood ash may reflect the photosynthetic pathway of the burnt woody species. Lastly, we show that the isotopic compositions obtained from wood ash prepared at different temperatures do not reflect a temperature dependent fractionation process, but a mixing line between calcite that formed by low temperature combustion and calcite formed by high temperature combustion which later underwent re-carbonation with atmospheric CO₂. In addition, we suggest that exchange processes may possibly occur during combustion between decomposing calcium-oxalate and atmospheric O₂, CO₂ and CO. The archaeological implications of this study are discussed in relation to identification of wood ash in the archaeological record, identification of fuel sources and burning temperatures, and diagenetic changes expected in karstic cave environments. The method presented here can be applied at any archaeological site.     

Stable isotope analysis of prehistoric populations from the cemeteries of the Middle and Lower Dnieper Basin,Ukraine

This paper presents the results of new stable isotopes (carbon and nitrogen) analysis of human, faunal and fish remains from thirteen cemeteries from the Middle and Lower Dnieper Basin, Ukraine. The results are integrated with earlier analyses, undertaken solely on human samples, in order to provide a comprehensive overview of subsistence across the Upper Palaeolithic through to Eneolithic periods in this region. This is the first time that a combined sample of human, faunal and fish remains has been studied in order to interpret subsistence strategies across these periods in Ukraine. The total dataset comprises 113 samples of human, faunal and fish remains, 59 of which include new analyses of faunal and fish remains that have not previously been made available for study (Table 1). The analysis of the faunal and fish remains allows for a consideration of trophic levels which indicates that the consumption of freshwater fish occurs from the Epipalaeolithic period onwards. Whilst the majority of the cemeteries and the individuals therein cluster in relatively tight groups, there is a significant offset between the human and faunal samples due to the consumption of freshwater resources. The fish samples analysed in the study appear to exhibit a relatively random distribution when compared to the other samples analysed. In general, freshwater resources remain significant through until the end of the Neolithic period and into the Eneolithic period at the sites investigated.     

Stable isotope analysis of 14 individuals from the Mesolithic cemetery of Vasilyevka II,Dnieper Rapids region,Ukraine

Bone collagen extracted from 14 humans from the Mesolithic cemetery of Vasilyevka II was analysed for their δ¹³C and δ¹⁵N ratios. This cemetery is one of only two later Mesolithic cemeteries from the Dnieper Rapids region, being dated to 7300–6220 cal BC on the basis of three radiocarbon determinations. This analysis provides insights into the nature of the diet of the Dnieper populations at the very end of the Mesolithic period, prior to the adoption of pottery in the region, and the assumed shift towards the exploitation of domesticates in the economies of the populations of the Dnieper region, and considers these in relation to broader temporal indicators of diet in the region.     

Stable isotope analysis of humans from Xiaojingshan site: implications for understanding the origin of millet agriculture in China

Millet agriculture originated in Northern China in the early Neolithic period (ca. 8000 BP), however, the actual importance of millet in human diets is still not clear. To determine the relative contribution of millet in human diets in this period we undertook stable isotope analysis of humans from Xiaojingshan site and fauna from Yuezhuang site, both of which are attributed to the Houli Culture and date to about 8000 years ago. The carbon isotope values of human bone collagen showed that millet (as a C₄ plant) only contributed approximately 25% of dietary protein, with the rest from C₃ based plant and animal sources, if a simple mixing model is used. We did not observe any statistical dietary difference between males and females at the site, although it has been argued that the Houli Culture was a matriarchal society. Finally, we compared our data with other published isotopic data from the contemporary Jiahu site and Xinglongwa site and a number of sites from the subsequent Yangshao Culture and found that millet only became a significant source of dietary protein approximately 1000 years later, as human carbon isotope values from these later sites indicated that almost all of dietary protein came from C₄ (i.e. millet) sources.     

Determining Olmec maize use through bulk stable carbon isotope analysis

Bulk stable carbon isotope analysis on absorbed organic residues in ceramics can be an effective method for discerning patterns of maize use when the ceramics come from relatively uniform archaeological contexts. The bulk stable carbon isotope method is faster and less costly than the more commonly used compound-specific stable carbon isotope analysis. Moreover, the bulk stable carbon isotope method can determine the presence of C4 plant carbon in samples in which organic compounds have degraded. Bulk stable carbon isotope analysis was used to discern patterns of maize (Zea mays mays) use among a sample of 24 ceramic sherds from an Early Franco Period feasting deposit (ca. cal 650 B.C.) at the Olmec site of San Andrés, La Venta, Tabasco, Mexico. A comparison of the δ 13C results of different categories of ceramics showed that proportionally more maize was used in luxury beverage service wares than in utilitarian vessels, suggesting that maize-based beverages were prominent in this probable elite feasting episode.     

Stable isotope analysis of Late Upper Palaeolithic human and faunal remains from Grotta del Romito (Cosenza), Italy

Stable isotope analysis of carbon (δ¹³C), nitrogen (δ¹⁵N) and sulphur (δ³⁴S) were carried out on one of the largest assemblages of Late Upper Palaeolithic human remains in Southern Europe, at Grotta del Romito (Cosenza), Italy. The burials were stratigraphically dated from ca. 18,000 to 13,000 cal BP, which was confirmed by a series of new AMS dates made directly on the bone collagen. Dietary reconstruction from carbon and nitrogen stable isotopes revealed that eight of the nine individuals analysed, dating to the Final Epigravettian, had very consistent diets, rich in terrestrial animal protein, regardless of their age or sex. These included two individuals who were suffering from severe pathologies. A single individual, dating to the Evolved Epigravettian had a more variable diet, which was significantly enriched in protein from marine or freshwater fish compared to the later burials. Overall, the results are consistent with the very limited number of other studies which describe a change to more specialised and less variable subsistence strategies, in this case the hunting of large herbivores, towards the end of the Palaeolithic period. Sulphur isotope values of all of the nine burials and several faunal samples were notably consistent, showing no evidence of long-distance migration to the site from a different geological zone.     

Stable isotope analysis of human bone and ethnohistoric subsistence patterns in Tierra del Fuego

Ethnohistoric records from Tierra del Fuego suggest that precontact Fuegians could be subdivided into three major groups: the Yamana, maritime hunter-gatherers of the Beagle Channel and islands to the south; the Selk’nam, terrestrial hunter-gatherers of southernmost Patagonia; and the Haush, a little-known group that seems to have combined elements of both Yamana and Selk’nam lifeways. However, the observed ethnographic patterns reflect societies whose way of life was significantly altered by European contact, habitat alteration, and exploitation of some of the key resources upon which Fuegian peoples were historically dependent. To test the linkage between ethnohistorically recorded subsistence patterns and prehistoric lifeways in the region, stable carbon and nitrogen isotopes were assayed from human burials that date within the last 1500 years before European contact. Isotopic analyses substantially confirm the ethnohistorically documented patterns, but also reveal some anomalies, such as Yamana populations who may have been more dependent on terrestrial resources (i.e., guanaco). Data from the Haush region suggest primary dependence on marine resources, like the Yamana, while the Selk’nam demonstrate limited use of such resources. Stable isotopic analysis can thus be used to test hypotheses concerning the validity of archaeological and ethnohistoric data.     

Stable carbon isotope ratios and their relevance to the determination of accurate radiocarbon dates for lime mortars

Attempts have been made to date mortar by radiocarbon analysis. Potential problems are discussed, and the possible use of stable carbon isotopes to detect contamination by geological carbonates is investigated.     

Stories behind the fortress: Stable isotope analysis and 14C dating of soldiers' remains from the Bayanbulag site,Mongolia

Stable carbon and nitrogen isotope analysis of 15 individuals buried closed to Bayanbulag site or the Shouxiangcheng fortress in southern Mongolia revealed complex dietary features. Only one individual had a diet similar to that of the pastoral population in northern China, while the others, who had varying carbon isotope values and low nitrogen values, might have been from different agricultural areas. ¹⁴C dating results suggest that the earliest interment could be much earlier than the building of Shouxiangcheng fortress recorded in Han texts. Analysis of the burial arrangement further confirmed that these deceased were not buried at the same time.     

Stable isotope analysis of human and animal remains from the Late Upper Palaeolithic site of Balma Guilanyà, southeastern Pre-Pyrenees,Spain

Stable isotope analysis of carbon (¹³C/¹²C) and nitrogen (¹⁵N/¹⁴N) was performed on collagen extracted from three human and five herbivore bone and tooth samples from the Late Upper Palaeolithic site of Balma Guilanyà (Catalonian Pre-Pyrenees, Spain). Contextual and palaeoecological data as well as radiocarbon dates indicate that the studied occupation phase took placed during the Bolling/Allerod interstadial (GI-1a event). The human remains were co-mingled without any anatomical association, corresponding to a minimum number of three individuals, and it was not possible to determine if the three analyzed samples are from one or more individuals. The mean isotope values obtained from the human remains are δ¹³C = −19.8‰ and δ¹⁵N = 6.7‰, while those of the large herbivores (red deer and wild goat) were −19.8‰ and 1.7‰ for δ¹³C and δ¹⁵N respectively. This indicates that the main source of protein in the diet of the Balma Guilanyà human(s) came from terrestrial herbivores. There is no zooarchaeological or isotopic evidence for the consumption of freshwater or marine resources at the site, which lies 80 km from the present Mediterranean coast. The low δ¹⁵N values observed in both human and animal samples correspond to a trend reported by other researchers working in northwestern Europe: a significant δ¹⁵N reduction in collagen from bones datable within 20,000–10,000 BP, followed by a rise to present values in the Early Holocene. This phenomenon, generally attributed to climatic and/or pedological processes, had not been previously observed in the Mediterranean region and, until now, was thought to be restricted to northern Europe.     

Late Holocene Neotropical agricultural landscapes: phytolith and stable carbon isotope analysis of raised fields from French Guianan coastal savannahs

This paper summarizes phytolith analyses from four pre-Columbian agricultural raised-field sites of the coastal savannahs of French Guiana—Savane Grand Macoua, Piliwa, Bois Diable and K-VIII—and carbon isotope analyses from the first-named site. The combined phytolith and ¹³C isotope analyses evidence the transformation of the landscape from a relatively homogeneous wetland vegetation comprised of a mixture of C4 and C3 plants (the latter including Cyperaceae and other herbaceous monocots such as Marantaceae and Heliconia, Oryzoideae grasses, and other plants typical of frequently flooded areas) to the construction of raised fields that were dominated by C4 plants (maize and other Panicoideae grasses). Our analysis proves the utility of phytoliths for tracing the agricultural history of landscapes, showing that, as in other parts of the Central and South American lowlands, maize (Zea mays) was one important crop cultivated in raised fields. We also estimated the productivity of raised-field agriculture, showing that in combination with other subsistence activities, it certainly had the capacity to sustain sizeable populations.