DNA analysis of archaeological sheep remains from China

Recent research has thrown considerable light on the history of the domestic sheep, but has not extended to ancient sheep specimens. In the present study, ancient DNA analysis was carried out on eight archaeological sheep remains recovered from Erlitou archaeological site in Henan Province (ca. 2100–1800 B.C.) to explore the genetic structure of ancient sheep and the phylogenetic relationship between ancient and modern sheep. We analyzed the control region sequences and coding regions of mitochondrial DNA from the remains by direct sequencing and restriction fragment length polymorphism analysis, respectively. Our results reveal that all ancient sheep belong to lineage A defined by modern sheep sequences. Phylogenetic analysis shows that neither argali (Ovis ammon) nor urial (Ovis vignei) mtDNA is closely related to Erlitou ancient sheep. In addition, our results suggest that ancient DNA analysis can serve as a powerful tool in tracing prehistoric population movement.     

Modeling wood acquisition strategies from archaeological charcoal remains

Archaeological charcoal remains are often used to reconstruct local woodland composition in the past, but rarely address how and why people may have selected specific woody taxa for particular purposes. Models from the field of human behavioral ecology predict that people forage for wood resources by taking into account the relative usefulness, abundance, and handling time related to procuring different wood types. Archaeological and ecological data from the site of Gordion, in Central Anatolia (modern Turkey), were used to test expectations associated with such models. Results suggest that inhabitants of Gordion used wood types for fuel in proportion to their local availability, but that they selected specific, more distant woods for construction. In most occupation periods pine was preferred for construction, perhaps because it produces long, straight timbers for roofing, despite the distance at which it grows from the site. This case study demonstrates that behavioral ecology modeling can help to distinguish between multiple wood acquisition strategies potentially used in the past and improve our understanding of wood use from archaeological charcoal remains.     

Detection of exposed and subsurface archaeological remains using multi-sensor remote sensing

Multi-sensor airborne remote sensing has been applied to the Itanos area of eastern Crete to assess its potential for locating exposed and known buried archaeological remains, and to delineate subsurface remains beyond the current limits of ground geophysical data in order to permit future targeted geophysical surveys and archaeological excavations. A range of processing techniques (e.g., Reed–Xiaoli anomaly detection) have been applied to the CASI, ATM and lidar data in order to detect anomalies based on the premise that buried remains are likely to alter the physical and chemical characteristics of the soil compared with those of the surroundings due to variations in soil depth and drainage. Through a combination of CASI, ATM and lidar data, surface remains have been classified and mapped effectively using an object-oriented approach. The detection of subsurface remains is more problematic; however, the thermal data is most promising in this respect. The value of capturing multi- or hyperspectral data at a high spatial resolution has been demonstrated as well as the additional benefits of combining these with airborne lidar.     

Genetic analysis of archaeological wood remains: first results and prospects

A total of 51 ancient oak wood samples originating from various European archaeological sites, dating from the Neolithic period to the 18th century, were assayed for the presence of reproducible chloroplast (cp) DNA sequences. Five polymorphic chloroplast fragments were targeted. Only five of the samples could be fully genetically characterised, revealing four different oak cpDNA haplotypes. In all cases, the haplotypes detected on ancient woods and the haplotypes characterised from fresh samples from the same localities matched. Overall, this congruence is consistent with a genetic continuity between ancient and modern European oaks, confirming the hypothesis that the mapped genetic patterns largely reflect the original structure that established during the post-glacial. This stability of the genetic structure implies that, in the future, the technique could be used to infer or confirm the transport of wood by man, providing interesting perspectives for the genetic analysis of ancient woods.     

The identification of charred archaeological remains of vegetative parenchymous tissue

The biased way in which some inferences may be drawn from archaeobotanical assemblages, due to problems in identification, is briefly discussed. A potentially important class of plant remains, vegetative parenchymous organs, commonly sources of food, are indicated as being such a class rarely identified from archaeological contexts. Their identification based on characters of the plants' anatomy and morphology together with an understanding of the way in which tissues degrade on preservation by charring is suggested. Characters of the charred tissues that make up vegetative parenchymous organs are described in detail. The processes of sorting and identification are then briefly discussed.     

Ancient DNA typing of archaeological pig remains corroborates historical records

The recent increase in both the abundance and taxonomic range of DNA sequence data in public repositories makes it possible to determine the maternal origin of lineages of faunal archaeological material by characterizing its mitochondrial DNA. Among the most commonly represented taxa are domesticated animals, for which extensive genetic characterization has revealed high levels of genetic diversity and (in at least some cases) strong phylogeographic clustering. Such information has significant implications not only for characterizing important aspects of the occupation history of archaeological sites, but also in providing novel insights into colonisation history and the scale and scope of trade and exchange networks. This can be done through studying the origins and dispersal of proxy organisms such as commensal and domesticated animals, as well as economically important wild fauna. To illustrate this approach, we compare historical records of maritime movement of people and pigs from two sites on Lord Howe Island, Australia, to phylogeographic results of DNA extracted from pig bones.     

The recovery and interpretation of microvertebrate bone assemblages from archaeological contexts

The analysis of microvertebrate remains recovered from buried contexts is frequently problematic for archaeologists. Diminutive size is a major obstacle to recovering, identifying, and interpreting microfaunal bone materials successfully. Their taphonomic history is often obscure, thus the significance of microvertebrate accumulations for settlement, subsistence, and ecological interpretation is difficult to evaluate. Recognizable and reliable archaeological signatures are sought for assessing the effect of taphonomic history on microvertebrate assemblages. Relevant signatures of bone damage, skeletal survivorship, taxonomic composition, and context are examined in assemblages produced and modified through accidental non-predator-related death, predator accumulation, and postmortem alteration.     

Recovery of DNA from archaeological insect remains: first results,problems and potential

We report the recovery of short fragments of PCR amplifiable ancient DNA from exoskeletal fragments of the grain weevil Sitophilus granarius (L.) extracted from Roman and medieval deposits in Northern England. If DNA preservation in archaeological insect remains is widespread then many applications in the spheres of evolutionary studies and archaeology can be conceived, some of which are outlined.     

Collagen fingerprinting of archaeological bone and teeth remains from Domuztepe,South Eastern Turkey

We applied a recently developed method of collagen peptide mass fingerprinting for taxonomic identification in faunal remains to 111 specimens from the Neolithic site of Domuztepe in southeastern Turkey. Advances on the published technique allow us to move beyond the key domesticated fauna (sheep, goat, pig and cattle) to include non-domesticates (deer and gazelle, as well as humans). As the animal economy of Domuztepe was dominated by domestic sheep and goats, a large number of the samples tested were morphologically indistinguishable sheep/goat remains. A number of samples represented elements that provide information on age (i.e., mandibles) or sex (pelvis); thus, this represents the first major study to attempt to improve kill-off profiles using collagen fingerprinting. We also show that the collagen extracted from dentine is equally amenable to the proposed methodology as bone collagen.     

The cost-effectiveness of certain methods of recovering macroscopic organic remains from archaeological deposits

In a survey, carried out to investigate the cost-effectiveness of the main methods used to recover macroscopic organic remains from archaeological deposits, it was found that sampling and recovery methods employed varied widely between individual workers. Sample type, recovery method and experience of the worker influenced the time taken to produce identified material, but it was clear that this is an expensive activity which must be justified in terms of information output.     

Species identification of archaeological dung remains: A critical review of potential methods

Abstract

Dung, macroscopically recognisable as such or not, can more commonly be found in archaeological contexts than is perhaps realised. Up to now, identification of dung to the species which produced it is usually either tenuous, or is not possible. However, species identification can be very informative and is necessary before any further studies can be conducted on the dung, for example on health and hygiene in the past and palaeoecology. This study presents a review of potential methods by which species identifications of archaeological dung can be undertaken. Criteria for identification can be divided into three broad categories: morphometric features of the dung; the content of dung and contextual evidence. Overall, the chances of a precise identification are high; however, a combination of different criteria and techniques will often be necessary to establish a secure identification. Moreover, preservation issues may exclude the application of some criteria while several criteria require more research and the expansion of reference collections of recent material. The overall aim is to move towards standardised methods for species identification of archaeological dung.     

Ancient bacterial DNA (aDNA) in dental calculus from archaeological human remains

The purpose of this study was to identify reactive bacterial aDNA in archaeological human dental calculus. Dental calculus was collected from a middle/late Neolithic human skull from Hulbjerg passage grave, Langeland, Denmark and prepared for transmission electron microscopy (TEM) or gold-labeled antibody TEM. TEM showed calcified, as well as non-calcified bacteria. Immunogold labeling occurred over the cytoplasmic portions of the sectioned bacteria. The result demonstrated that it is possible to identify aDNA sequences from bacteria in archaeological material of considerable age by this technique.     

Subsistence and symbol: the interpretation of bird remains in archaeology

This issue of the journal is devoted to papers on the role of birds in archaeology. Most of the contributions are based on papers given at the second meeting of the Bird Working Group of the International Council for Archaezoology (ICAZ) held at the University of Southampton (UK) in September 1995. The meeting brought together archaeologists, zoologists and ornithologists, and the subjects discussed cover most of the relationships between humans and birds that can be established form the study of birds from archaeological sites.     

The ethnoarchaeology of firewood management in the Fang villages of Equatorial Guinea, central Africa: Implications for the interpretation of wood fuel remains from archaeological sites

In archaeological literature, the study of trees and wood remains is a topic of relatively marginal interest, especially compared to texts on crops and human–animal relations. However, charcoal is the most frequent botanical remain found in archaeological sites. Charcoal analysis can therefore play a major role in the development of studies in both landscape and palaeoethnobotanical reconstruction. The majority of the archaeological charcoal assemblages reflect the exploitation of wood as an energy source (fuel). The archaeological study of firewood selection has been predominantly developed from “eco-utilitarian” or “subsistence economy” perspectives, but has not yet considered fuel collection and use as one of the most enduring categories of human–environment interactions, nor has archaeology looked into its potential as a source of empirical information on past perceptions of, and interactions with, ancient landscapes. The aim of this paper is to expand previous archaeological work on the interpretation of charcoal macro-remains through the study of firewood collection as a historically constituted, socially mediated and archaeologically observable landscape practice. In order to achieve this, we present an ethnoarchaeological case study from the Fang society of Equatorial Guinea (central Africa) aimed at gaining a better understanding of the complex interactions between cultural, ecological and economic variables in firewood collection strategies.     

Optimum temporal and spectral window for monitoring crop marks over archaeological remains in the Mediterranean region

This paper aims to explore the formation of crops marks over archaeological features using remote sensing data. Crop marks are very important to archaeological research since they can reveal in an indirect way buried archaeological remains. Several researches have been able to locate subsurface relics via interpretation of multispectral and hyperspectral satellite images. However the best phenophase (time-window) where these crop marks are enhanced and which spectral bands are the most capable for this enhancement are still an open question for the scientific community. This study aims to address these fundamental aspects of Remote Sensing Archaeology. For this reason an extensive dataset was used: two control archaeological sites were constructed in two different areas of Cyprus (Alampra and Acheleia). In these areas both barley and wheat, were cultivated. In situ spectroradiometric measurements were taken over a whole phenological cycle (2001–2012) for both areas. More than 30 in situ campaigns were performed and more than 2600 ground measurements were collected. The phenological analysis of these measurements have shown that a period of only 15 days is best suitable for monitoring crop marks which is connected with one phenological stage; during the beginning of the boot stage of the crops. This period can be used in areas having similar climatic condition as Cyprus (Mediterranean region). Also as it was found, this phenological stage of crops might be also influenced by the climatic conditions of the area. The evaluation of a proposed Archaeological Index was performed at the Thessalian plain (central Greece), where several Neolithic settlements are established. The evaluation was made using both Hyperion and Landsat images. The results have shown that the proposed Archaeological Index is suitable for the enhancement of crop marks in satellite imagery.     

A new calibration of the XRD technique for the study of archaeological burned human remains

A new calibration of human bones as a function of programmed temperature (200–1000 °C) and time (0, 18 and 60 min) is presented and discussed in order to investigate the issues related to the study of cremated bone remains by using the powder X-ray diffraction approach. The experimental results confirm the growth of hydroxylapatite crystallites as a function of the applied temperature, with a sigmoid behaviour that has been parameterized to the experimental data points. Particularly, it was observed that the thermal treatments for 60 min anticipate of about 100 °C the effects that are otherwise observed after the treatments for 0 min. The developed procedure was subsequently applied to cremated remains of various archaeological sites of Spain and supplied precise information not only about the temperature reached during the funerary rites, but also on the presumed duration for the cremation.     

New methods for the identification of evidence for bitting on horse remains from archaeological sites

This article describes alterations to equid lower second premolars and diastemata from a series of known life history equids and a number of archaeological horse specimens from the British Iron Age. Two new methods for recording bit wear are proposed involving the analysis of the extent and morphology of enamel/dentine exposure on the anterior edge of LP2s and analysis of the extent of new bone formation and bone loss to the diastema of the mandible. It is suggested that when a bit is used on a horse it acts more frequently on the anterior margin of the LP2 than has previously been thought and that repeated contact between the bit and the LP2s and diastemata results in recognisable damage to these areas of the mouth.