Rice archaeological remains and the possibility of DNA archaeology: examples from Yayoi and Heian periods of Northern Japan

Japanese rice cultivation in paddy fields has 2,400∼3,000 years of history. Most of modern Japanese rice varieties are classified as Temperate-japonica (Tm-J). Few landraces are recognized as Tropical-japonica (Tr-J) only in southwestern Japan. However, ancient DNA studies and phytolith analysis suggest that Tr-J strains were more popular in the past than now. Maekawa is a complex archaeological site composed of paddies dated from the Yayoi (2,100 years BP) to the Heian (1,100 years BP) periods. Phytolith analysis indicates that intensive rice cultivation was practiced in both periods, but there was no cultivation in the intervening period. Morphological features of bulliform phytoliths suggest that Tr-J was cultivated during both periods. Locally, rice cultivation during the Heian period was brought to a close by a flood event, in which immature rice plants were pulled down and buried in silt to be preserved in a quasi-carbonized/ waterlogged state. Ancient DNA (aDNA) analysis of the carbonized plant culm from Heian Maekawa recovered chloroplast DNA sequences of the 6C7A plastid subtype, which is common to both Tr-J and Tm-J, whereas two plastid subtypes, such as 6C7A and also 7C6A, were found in aDNA of carbonized grains from the Tareyanagi site of the Yayoi period. The latter plastid subtype was specific only to Tr-J. In order to better characterize the past rice populations, modern landraces collected in the local area were classified with morphophysiological traits. Some of the landraces were found to carry several traits of Tr-J, including bulliform phytolith types, but mixed with Tm-J traits. Based on the discontinuous distribution of rice phytoliths between the Yayoi and the Heian period, the early introduction of rice cultivation may have been discontinuous and locally reintroduced after a ∼1,000-year hiatus, but with a genetically different rice population. Such populations were composed from Tr-J like strains as shown by landraces but with reduced diversity in plastid types. Through such changes, since the Yayoi era, Tr-J was largely replaced by Tm-J, although ancient Tr-J continued to participate in the genetic makeup of later rice populations and may have aided the local adaptation of introduced Tm-J.     

Nonmetric dental characteristics of the early modern population of Okinawa Island in Nansei Islands,Japan

Dental morphological characteristics of the early modern population (c. 17–19th century AD) of Okinawa Island, Nansei Islands, were investigated to clarify their genealogical characteristics. We observed and classified 25 nonmetric traits of tooth crowns and roots from human remains (106 individuals) excavated from tombs of the early modern period in Okuma, Ginowan City, Okinawa. The incidences of these traits were compared with the incidences in the populations of the other Nansei Islands, of Japan, and of Asia overall. Univariate analysis of each trait and multivariate biological distance analysis based on the frequencies of the traits showed that the Okinawa population in the early modern period more closely resembled the migrant Yayoi populations than it did the native Jomon populations. It is difficult to support the “Ainu‐Ryukyu common origin theory” with regard to the early modern population in Okinawa without some modification of the theory, as well as the modern populations in Tanegashima and Okinawa Island. The geographical cline in the modern period from the northern Kyushu to the Okinawa Island via Tanegashima was confirmed in this analysis. Considering the major temporal changes in northern Kyushu and Tanegashima in addition to the geographical cline, the southward gene flow of the migrant Yayoi elements from northern Kyushu to the central Nansei Islands via the northeast end of the Nansei Islands is suggested. In addition, this study detected some temporal changes from the early modern to the modern period in Okinawa Island. Although the minor temporal change may be attributed to some genetic drift, gene flow from the Japanese main islands or China might be considered one of the causes of the change. Copyright © 2010 John Wiley & Sons, Ltd.     

Frequencies of Rare Incisor Variations Reflect Factors Influencing Precontact Population Relationships in Mexico and the American Southwest

Mexican prehistory is characterized by migration and population isolation in its earliest phase, followed by later inter‐population cultural interactions, such as politics and trade. While shared, common morphological variants are often informative about recent population events, rare trait variants have not been widely investigated to see whether they might be informative about earlier events. Here, we consider populations that show several rare variants at frequencies high enough to warrant such an investigation. Examining past population structure can help us understand population interactions across different periods of time, levels of migration, and population isolation/drift. Multiple‐origin populations may have more variation, including more and higher rates of rare traits. We examined maxillary lateral incisors in 1117 dentitions from 76 samples of Preclassic/Classic and Postclassic pre‐European contact Mexico and the American Southwest for the prevalence of rare lateral incisor variants. Variants observed included barrel, congenital absence, interruption groove, mesial marginal bending, peg, reduced, supernumerary, and talon teeth. The most common variant overall is interruption groove. Central Mexico, Huasteca, and the Lowland Yucatan region samples present the highest overall number of incisor variants. Higher frequencies of single unusual traits are commonly found in samples from smaller populations, while samples from larger population centres show a greater range of these rare variants. We investigated to see whether the pattern of rare incisor variant frequencies reflected early or later population history by comparing similarity/distance matrices and factor model matrices using Mantel tests and Generalized Procrustes analysis. Results show a weak relationship with the Postclassic period and shared migration/language and trade interaction models. We suggest that later cultural interactions have acted to mask earlier population history. Research on serial founder effects should be approached multiregionally and across time, to avoid missing inter‐region biological relationships. Copyright © 2015 John Wiley & Sons, Ltd.     

What can we make of these fragments? Excavation at ‘Mamilla’ Cave,Byzantine period,Jerusalem

This paper reports on the pathology and discrete traits of 1388 vertebrae uncovered from a mass burial site at ‘Mamilla’ cave, Jerusalem, dated to the Byzantine period. Observations of the vertebrae were aimed at reconstructing an anthropological profile of the population, by describing such factors as the frequency of pathology and discrete traits, and age at death of the population. The results show that most of the victims were relatively young, and that they differed significantly in their epigenetic traits from Jews and nomadic Arabs. The results reinforce earlier assumptions that the skeletal material excavated at Mamilla cave represents the Christian population of the city, massacred by the Persians in 614 ad . Copyright © 1999 John Wiley & Sons, Ltd.     

Etruscan biology: the Tarquinian population,seventh to second century BC (Southern Etruria,Italy)

The aim of this study was the anthropological analysis of an ancient Etruscan population from Tarquinia (Viterbo, Latium, Italy). The chronological period is the seventh to second century BC. Nine discrete traits were scored. The results show the complex situation of the Etruscan sample compared to some coeval Mediterranean populations. A strong endogamy probably existed within the upper levels of the Tarquinian society, and there was probably a biological continuity with the present Tuscans. © 1997 John Wiley & Sons, Ltd.     

Reconstruction of Socioeconomic Status in the Medieval (14th–15th Century) Population of Grevenmacher (Luxembourg) Based on Growth,Development and Diet

Numerous historical sources describe many aspects of the estate‐based society in medieval times; the detection of socioeconomic status within populations through skeletal remains is a topic of growing interest in anthropological studies. In medieval times, it was common for members of high social rank to be buried within or next to a church. This was certainly the case in Grevenmacher (Luxembourg), where remains of a church building and an adjacent cemetery from the 13th until the beginning of the 15th century were subjected to archaeological and anthropological analysis. By integrating archaeological and anthropological elements, as well as stable isotope analysis, we documented osteological manifestations as indicators of diet differentials in two subsamples (first group consists out of 56 individuals, second one out of 184 individuals) from the medieval cemetery of Grevenmacher. We could distinguish two subsamples that we assumed as different in their socioeconomic status with regards to the burials' position in the cemetery and burial characteristics. Differences in osteological traits such as bone length, stature and body mass indicated differences in diet between sample groups. To substantiate these outcomes, stable isotopic analysis (δ¹³C, δ¹⁵N) were made; the results displayed a clear separation between the sample groups on the basis of their diet. Therefore, we were able to confirm certain individuals in the medieval population of Grevenmacher in relationship to their socioeconomic status. Copyright © 2017 John Wiley & Sons, Ltd.     

MIGRATION AND SELF‐SELECTION: MEASURED EARNINGS AND LATENT CHARACTERISTICS*

ABSTRACT Research in regional and labor economics has established that economic incentives play a significant role in the process of internal migration. The most common approach is to view migration as a form of human capital investment undertaken by individuals who expect to benefit from the standpoint of increased earnings. One of the central concepts in these models is self‐selection. Individuals who self‐select the option of migration tend to differ from the nonmigrant population in ways that are not measured in most data sets. The contribution of this paper is in its distinction between two aspects of migrant selection. On one hand, some workers possess unmeasured traits that might simultaneously affect their wages and their propensity to engage in risky human capital investment such as migration. On the other hand, measured earnings might exert a direct effect on migration. Based on samples of employed Swedish males and females at two points in time, this study seeks first to examine whether migration between the two periods occurs in the presence of correlation between unmeasured factors present in both earnings during the first period and the subsequent decision to migrate. Second, it looks for an explicit role of earnings per se in the migration decision. Results of the study provide support for selection based on unmeasured traits for both genders. For females, there is also evidence of selection based on measured earnings.     

Craniometric evidence for South African Later Stone Age herders and hunter–gatherers being a single biological population

Later Stone Age (LSA) hunter–gatherers and herders co-existed in South Africa during the last 2000 years. In spite of being the focus of intensive research over the years, the biological status and origins of the herders are still unclear. Did they represent a genetically distinct immigrant population who remained separate from the indigenous hunter–gatherers, or where they indigenous hunter–gatherers who took up herding after contact with herders, probably in northern Botswana? Here, this issue is investigated using craniometric data collected on a large sample of individually dated human crania from coastal LSA context. Mahalanobis distances (D), calculated from the raw metric data, show that there was a small increase in inter-individual craniofacial variation after the introduction of herding at ca. 2000 BP. Here it is argued that this small increase in variation is neither consistent with a large-scale immigration of genetically distinct herders into South Africa, or the long-term co-existence of two genetically distinct populations. Two alternative explanations fit the data better: (1) herding entered South Africa via the small-scale immigration of genetically distinct herders; and (2) local hunter–gatherer populations adopted herding after coming in contact with herders in northern Botswana. While small-scale immigration would not have had a major influence on the local gene pool, it would have increased variation to some extent as immigrants mixed with local populations. If small-scale external gene flow was not a factor in the introduction of herding, secular issues related to the introduction of herding could explain the increased variation in post-2000 BP populations.     

The nation‐state in its state‐istics (Belgium, 1846–1947)

Statistics are, as the etymology of the term suggests (state‐istics), intimately connected with the construction or administration of the nation‐state. This paper addresses the genesis and development of the nation‐state by studying one of the main instruments that states use to ‘embrace’ their populations, viz. population statistics. More particularly, the paper presents a critical analysis of the conceptual and ‘scientific’ representations of modes of belonging to the nation‐state as produced in the Belgian (Queteletian) population censuses from the mid‐nineteenth until the mid‐twentieth century. It is shown how the analyses of the statisticians' interests, techniques and classification schemes shed light on the various ways in which inclusion in, or exclusion from, the Belgian nation‐state have been articulated in its population censuses. It is argued that these shifting interests and classification schemes also inform us about the construction and administration of the contemporary nation‐state.     

An exploration of character traits and linear measurements for sexing mountain gazelle (Gazella gazella) skeletons

The skeletal elements of mountain gazelle (Gazella gazella) have proven difficult to sex with statistical confidence due to wide overlap in the body size of the two sexes. We studied a sample of 53 modern mountain gazelle skeletons to determine which character traits and metric measurements best predict sex. The success of the character traits was determined using blind tests while the metrics were examined using discriminant function analysis. The most useful elements include the previously identified horn core, pubis and atlas, but also some new bone portions that preserve well in the archaeological record (e.g., distal tibia, distal metacarpal and metatarsal, and second phalanx). Surprisingly, two elements commonly used in sexing analyses (distal humerus and astragalus) were not among the most effective elements. Although cutting points and discriminant functions for sexing gazelle bone portions are presented here, they do not account for potential body size change and thus are not suited for direct application to archaeological assemblages. Instead, we provide guidelines for application to archaeological gazelle assemblages, most importantly a regression analysis that considers the sex ratios obtained from multiple measurements to predict the sex ratio of archaeological gazelle populations.     

IN‐SITU ELEMENTAL AND Sr ISOTOPE INVESTIGATION OF HUMAN TOOTH ENAMEL BY LASER ABLATION‐(MC)‐ICP‐MS: SUCCESSES AND PITFALLS*

Trace element and Sr isotope data were obtained by laser ablation‐ and solution mode‐(MC)‐ICP‐MS analysis for tooth enamel from remains excavated at the New Kingdom period Egyptian colonial and Nubian cemetery site of Tombos (Sudan). Elemental abundances determined by both methods of ICP‐MS analysis yielded comparable values; however, ⁸⁷Sr/⁸⁶Sr values obtained by laser ablation were higher compared to their solution mode counterparts. This discrepancy is related to the production of a molecular interference—Ca + P + O (overlaps ⁸⁷Sr); hence the higher ⁸⁷Sr/⁸⁶Sr values recorded during ablation analyses. Laser ablation studies of enamel may provide relatively precise ⁸⁷Sr/⁸⁶Sr values rather quickly but cannot be used for accurately deciphering historical population migrations.     

The Occurrence and Possible Aetiology of Spondylolysis in a Pre‐contact California Population

The occurrence of spondylolysis (neural arch separation at the pars interarticularis) is reported in a pre‐contact (720–550 Cal BP) Central California population, CA‐CCo‐647 (n = 46). Spondylolysis was examined within this population to assess differences in sex, age, and possible relationships with biological affinity. Furthermore, prevalence rates of spondylolysis were compared across various populations to discern the aetiology of the defect. Within CA‐CCo‐647, spondylolysis was found in 17.4% (8/46) of the population. All occurrences are complete, bilateral separations of the neural arch in the lower lumbar (L4 and L5). The defect is not seen in individuals below the age of 18, and there is no correlation between adult age and spondylolysis. Male individuals display the defect at a higher rate (30.8% ‐ 4/13) than female individuals (12.1% ‐ 4/33); however, this is not a statistically significant finding. There does seem to be a correlation between an individual's sex and the onset of the defect; male individuals display spondylolysis at a younger age than female individuals. This finding could represent differences in activity patterns among young male and female individuals. In a biological distance analysis using craniometric data, male and female individuals with spondylolysis clustered closely, suggesting a genetic component for the defect. Finally, significant differences were found in spondylolysis prevalence between various populations representing distinct geographic and temporal settings. Significant differences were found among Native pre‐contact samples and even between two comparable pre‐contact California skeletal samples. These populations all engaged in distinct activities and were likely composed of genetically distinct groups of individuals, which may account for the differences in spondylolysis prevalence. All of these findings, both within CA‐CCo‐647 and between the various samples, suggest that the aetiology of spondylolysis is likely an interaction of genes and activity. Copyright © 2012 John Wiley & Sons, Ltd.     

Dental and oral nonmetric traits in a Coimbra reference sample: testing intrasample chronological and spatial variation

A reference sample of dental and oral nonmetric traits should represent its biological population from which it stems. The presence of individuals born at different times, different regions, and separate countries in the Coimbra-identified cranial collections provides the test of whether this sample reflects the biological continuity of this Portuguese sample among the late modern (early industrialization, nineteenth century) to early contemporary (early demographic transition, first half of the twentieth century) population of this region of central Portugal. The Coimbra collections were scored for 61 traits using methodology by Hauser and De Stefano (1989), Turner et al. (1991), Scott and Turner (1997), Irish (1998), and Marado and Silva (2016). The 600 individuals in the sample were divided by generation, region, and nationality. Their phenetic diversity was tested with principal component analysis and with the mean measure of divergence statistic. The proximity between the subsamples was generalized, and it mimicked previous genetic marker results. Some small subsamples hindered conclusions; nevertheless, this Coimbra sample is considered a reliable dental reference sample for the Portuguese late modern/early contemporary population.     

Sensitivity of Dental Phenotypic Data for the Identification of Biological Relatives

Using modern samples of known kin, this paper evaluates two fundamental aspects of kinship analysis in archaeological contexts: (1) choice of data (dental metrics or morphology) and (2) analytical approach (multivariate, distance‐based approach or a ‘rare trait’ analysis). Stone dental casts were analysed from 155 female individuals from four sampling locations in Kenya. Of these 155 individuals, only three pairs were close kin: mother–daughter, sister–sister, and first‐cousin–first‐cousin dyads. After variable winnowing, inter‐individual distances or similarities were calculated using 11 odontometric variables and 25 dental morphological variables. Resulting distance matrices were ordinated in two dimensions using multidimensional scaling. Odontometric data performed relatively well at identifying known relative pairs, but the results were heavily affected by choice of similarity measure (e.g. Euclidean distances vs. Gower coefficients) and pre‐analysis data treatments (e.g. raw data vs. principal components). Dental morphological data performed comparably with odontometric data but were slightly less effective. Rare traits were identified and compared among relative pairs for concordance, with mixed results. Rare morphological features were randomly distributed throughout the population and were not exclusively found in close kin. In combination, results indicated the sister–sister dyad was most consistently identified; however, in no analysis were relatives more phenotypically similar than all random pairs of unrelated individuals. A multivariate, distance‐based approach was more effective than rare traits at identifying relative pairs, but even under ideal circumstances, there is not enough variation present in the dentition to faithfully identify close relatives in the absence of contextual archaeological data. Copyright © 2017 John Wiley & Sons, Ltd.     

Biological Homogeneity and Familial Segregation in the Iron Age Population of Alfedena (Abruzzo,Italy), Based on Cranial Discrete Traits Analysis

The Iron Age necropolis of Alfedena has been well-known since the last century. In this study 10 non-continuous traits of the cranial skeleton are examined in a series of 94 adult individuals (54 males and 40 females). The division of the Alfedena necropolis into tomb circles has permitted the investigation of the possible existence of familial segregation. Our analysis has shown us that the individuals inhumated in the different tomb circles have a common genetic substrate probably attributable to the strong endogamy present in this population.     

Flax look‐alikes: Pitfalls of ancient plant fibre identification

Plant fibres have been used since ancient times in numerous applications ranging from nets and basketry to textiles for clothing. A proper identification of plant fibre remains in archaeological excavations provides important information about resource exploitation and agriculture. In order to identify plant fibres, a series of fibre features—cross‐section diameter, lumen diameter, dislocations (nodes), cross markings, cross‐section shape and lumen shape—have been defined and characteristic traits of these features for different fibre types established. How suitable these traits really are for fibre identification has been a matter of debate. To resolve this issue, we have performed a systematic investigation of typical textile bast fibres: flax, nettle and hemp. We have investigated cross‐section diameter, lumen diameter, dislocations (nodes) and cross markings using standard compound, white light transmission microscopy. Our investigations show that all the traits that are considered characteristic for one type of plant fibre can also, on occasion, be found in other types. This demonstrates that an investigation of the traits listed above is not sufficient to ensure a correct identification of the plant fibre material; in particular, when only a small amount of material is available. This is often the case in archaeological excavations.     

Determinants of Variation in Population–Employment Interaction Findings: A Quasi‐Experimental Meta‐Analysis. 人口‐就业相互作用的决定因素研究：一种准实验元分析法

This article reports about a metaregression analysis of empirical results generated using data for the northern Netherlands (1988–2002) in order to investigate the ambiguity in results in the population–employment interaction literature. Specifically, the analysis deals with the issue whether “jobs follow people” or “people follow jobs.” The article starts with introducing the basics of quasi‐experimental meta‐analysis and with identifying some advantages of using quasi‐experimental meta‐analysis as compared with the standard meta‐analysis approach. Two subsequent sections document the selection of the population–employment interaction model and salient characteristics of the data set as well as the setup of the primary analyses. A total of 4,050 quasi‐experimental empirical results for the jobs–people direction of causality are generated using different specifications and estimators for a spatial econometric interaction model. The subsequent metaregression analysis reveals that the empirical results are largely shaped by the spatial, temporal, and employment characteristics of the data sampling. The results also appear much more sensitive to different measurements of the model's key variables when compared with alternative specifications of the spatial weights matrix. The main determinant driving empirical results about jobs–people causality are differences in model specification and estimation, as revealed by an inherent bias in parameter estimates and misguided inferences for some of the commonly used specifications. Finally, suggestions for future research are identified.     

传统地域文化形成中的人地关系作用机制初探——以徽州文化为例

人地关系是人文地理学重要的研究领域。徽州文化是农耕文化繁盛时期遗存的典型地域文化。本文主要结合对徽州地域文化的案例研究,剖析传统地域文化形成中的人地关系作用机制。论文认为,自然环境将影响地域人口社会、经济结构的特征,而人口的社会、经济结构则对地域文化的形成和发展有着关键性影响,因此,地域人口的社会、经济结构可以被视为人地相互作用中的关键性链接因素,优化人口结构将有助于区域文化的持续健康发展。     

URBAN‐TO‐RURAL POPULATION GROWTH LINKAGES: EVIDENCE FROM OECD TL3 REGIONS*

The objective of this paper is to understand, from an international comparative perspective, how population growth in rural regions is affected by the relationship with their nearby urban centers. By means of a cross‐sectional analysis on OECD small regions (Territorial Level 3), the paper distinguishes spread effects—positive spillovers arising from urban growth—from the net effect of distance to nonrural places. The results show that spread effects outweigh backwash effects, so that rural regions benefit from growth in urban places. A rural region's distance from urban and intermediate regions has a negative effect on its population growth rate. Nevertheless, both the strength of this effect and the growth spillovers decline with distance, and this occurs relatively faster in Europe. The results further suggest that proximity to large urban areas has a higher positive influence than proximity to intermediate areas, but only outside Europe.