Secular decline in cranioskeletal size over two millennia of interior central California prehistory: Relation to calcium deficit in the reconstructed diet and demographic stress

An osteometric study of residual rickets (RR) skeletal plasticity has been made of a time-ordered sequence of fifteen series of adult skeleton sets (n=359) from the Great Valley of central California (GV), spanning the three archaeological horizons of California Indian prehistory in this region: Early (EH), Middle (MH) and Late (LH). By least-squares linear regression analysis, a clear and continuous downward trend obtained in cranioskeletal size in both sexes, proceeding from EH to LH in the GV sequence, pari passu with a steady upward trend in the RR-15 score. To ascertain if dietary calcium deficit (CADEF), a known cause of active rickets, can explain the observed secular increase in RR, the aboriginal diet was reconstructed by the diet grid method: CADEF was found to rise from borderline in the EH, to moderate in the MH, to severe in the LH, as the subsistence base shifted gradually away from hunted prey, and storable seed foods—mainly leached acorn meal—became the dominant source of energy. Further regression analysis has shown that the secular upward trend in CADEF is highly correlated with the temporal clines of the GV sequence, directly with the RR-15 score, and inversely with cranioskeletal size: in round numbers, one grade of CADEF brings about a loss of 120 cm³ or 4 per cent in partial skeleton volume. Two biocultural influences—demographic stress (DS) in females, and the less common ‘male nutritional advantage interaction co-factor’ (MNAIC)—modulate the primary bone antitrophic CADEF effect: DS enhancing, MNAIC ameliorating.     

Cranioskeletal Size Variation in San Francisco Bay Prehistory: Relation to Calcium Deficit in the Reconstructed High-seafoods Diet and Demographic Stress

An osteometric–statistical study of residual rickets (RR) skeletal plasticity has been made of a time-ordered sequence of 11 series of adult skeleton sets (n=251) from shellmound sites around San Francisco Bay (the Bay) spanning the three archaeological horizons of Central California Indian prehistory in this region: Early (EH), Middle (MH) and Late (LH). To control for major differences of subsistence ecology and ethnolinguistic affiliation, the Bay sequence was further subdivided into four subregions: East (EB, Costanoan), South (SB, Costanoan, high acorns), West (WB, Costanoan, low acorns) and Northwest (NWB, Coast Miwok). In the eight EB series, a non-linear downward trend in cranioskeletal size was observed between EH and LH2, coinciding with a modest upward trend in the RR-15B score. To ascertain if food calcium deficit (CADEF) can explain the observed secular decline in cranioskeletal size, the evolving aboriginal Bay archaeodiet—relatively high in marine mammals, birds, fish and calcium-rich mollusks—was reconstructed by the diet grid method: in the three subregions with large stands of oaks (EB, SB, NWB), CADEF rose from negligible in the EH to moderate in the LH, as the subsistence base became more dependent on leached acorn meal for energy. Temporal and areal variation in Bay cranioskeletal size relates mostly to CADEF, demographic stress in females (DS), and the ‘male nutritional advantage interaction co-factor’ (MNAIC), in descending order of importance. This study has also confirmed the existence of the San Francisco Bay Indian physical type.     

Computed femur and tibia volumes and cranial capacity under near-optimal conditions of skeletal growth: Relation to geomagnetic dipole field intensity and other latitude-dependent climatic variables in archaeological North America and North Africa

Using computed femur (F) and tibia (T) volumes and cranial capacity (CC), the Pleistocene geomagnetic acromegalosis (PGMA) model of human evolution is tested in eight series of male skeleton sets (n = 123) from aboriginal North America and North Africa, the majority dated to the Holocene. The material was selected from the extensive multiracial osteometric data base previously assembled in the course of adult residual rickets (RR) skeletal plasticity research in archaeological populations of the Northern Hemisphere, controlling for optimal bone eutrophism, that is, for the absence of sunshine and calcium deficits and demographic stress. Six of the eight series are of hunter-gatherers, including a terminal Pleistocene mesolithic sample from the Maghreb. As predicted by PGMA, a clear osteotrophic response is observed between the F, T, and CC of these populations and their geomagnetic dipole field intensity (GMFI) backgrounds, here expressed in microteslas (μT); along with evidence that the GMFI-dependent osteotrophism is stronger near the sagittal plane. Slightly higher bioenvironmental correlations, also compatible with PGMA, are obtained with a composite environmental osteotrophism parameter (CEOT.J), which incorporates the reciprocal of the archaeological site's reconstructed mean July temperature as a synergizer of GMFI. In a final demonstration of the utility of the new computed osteovolumetrics, it is shown that Skhul IV, to judge from its size and proportions, is a hyperrobust pre-neanderthal male skeleton of early Late Pleistocene interglacial age, and belongs to the North African geographical race.     

Rural Rickets: Vitamin D Deficiency in a Post‐Medieval Farming Community from the Netherlands

Rickets is caused by vitamin D deficiency as a result of limited exposure to sunlight and inadequate diet. In the 19^th century, rickets was endemic in most northern European cities. In post‐Medieval Netherlands, rickets is documented in low frequencies in a few urban samples, but has not been studied in contemporaneous rural populations. Beemster is a rural farming community in the Netherlands that was established in the 17^th century upon drained land, with the Middenbeemster cemetery in use until 1866 AD. Ninety‐five individuals from the ages of 32 weeks in utero to 15 years were examined for rickets in order to understand factors that can cause vitamin D deficiency in rural, non‐industrialized populations. To identify rickets in the Beemster sample, ten features were scored, with bending deformities of the lower limb and one other feature, or at least three non‐bending features, having to be present in order for diagnosis. Nine individuals (9.5%) had evidence of rickets—a high prevalence, especially for a rural community where ample sunlight was available. The two and three year old Beemster infants were most heavily affected with an age‐specific prevalence of 30.4%. Two three‐month‐old infants also had rickets. Some of the affected may have developed rickets secondarily, as a result of a different illness, but cultural practices including prolonged swaddling, occlusive clothing, and keeping the young indoors, are suggested to have contributed to this high rickets prevalence. Dietary variables including poor weaning foods and common episodes of malnutrition may have also contributed to vitamin D deficiency. This study demonstrates the value of careful analysis of pathological conditions in subadults and highlights that rickets was not only a disease of cities, but affected populations that would appear to have been at low risk, because of maladaptive cultural practices. Copyright © 2013 John Wiley & Sons, Ltd.     

Rickets and child abuse: the case of a two year old girl from the 4th century in Lisieux (Normandy)

The burial of a child of two years of age from the 4^th century site of Lisieux‐Michelet (Calvados, France) was examined for bone hyperplasia and a variety of mechanical deformations recognized in association with skeletal trauma. Results of DNA analysis of bone using the PCR method identified this child as female. Microscopic features of the teeth (interglobular dentine) and a sample taken from the tibia conclusively determined that this child suffered from vitamin D‐resistant rickets and possibly X‐linked hypophosphataemic rickets as its most common form of occurrence. In addition, evidence for child abuse is suspected based on the presence of cranial and tooth fractures and the appearance of successive plaque‐like endocranial (meningeal) appositions. This is the first palaeopathological report of child abuse in antiquity. Copyright © 2002 John Wiley & Sons, Ltd.     

The Use of Hunting and Herding Spaces: Stable Isotope Analysis of Late Archaic and Early Formative Camelids in the Tulan Transect (Puna de Atacama,Chile)

We present the results of analysing stable carbon and nitrogen isotopes in camelid remains found at the Late Archaic site TU‐52 (ca. 5000–3800 bp ), the Tarajne Phase site TU‐94 and the Early Formative sites TU‐54, TU‐85 and TU‐122 (ca. 3100–2400 bp ). All of the sites are located in the Puna de Atacama (Northern Chile) along the 14 km Tulan transect, between the head of Tulan ravine (ca. 3000 m.a.s.l.) and the border of the Salar de Atacama (2317 m.a.s.l.). Our aim is to understand how the space was used by hunter‐gatherers and early herders from the beginning of camelid domestication to the consolidation of herding practices. Isotopic analyses were complemented with osteometric data in order to correlate changes in animal size and isotopic values with the initiation of animal husbandry. Isotopic and osteometric results show less variability of δ¹³C and δ¹⁵N values during the Late Archaic and Tarajne Phase, whereas variability is higher during the Early Formative. We postulate that during the latter period, there was more widespread use of hunting and herding spaces along the Tulan ravine, including areas above 3000 m.a.s.l. as result of more consolidated herding practices, while the highlands were used as a complementary space to hold livestock near ritual sites and residential settlements. Copyright © 2017 John Wiley & Sons, Ltd.     

A regional investigation of subadult dietary patterns and health in late Iron Age and Roman Dorset,England

This is the first regional analysis of the impact of Romanisation on subadult dietary patterns and related health parameters in Britain. A sample of 200 subadults from late Iron Age (LIA) and Romano-British (RB) Dorset were examined for dental health and specific metabolic diseases, and a sub-sample of 29 individuals were selected for nitrogen and carbon isotope analysis. The results showed that dental health declined in the Romano-British period and the incidence of scurvy and rickets rose. Increased consumption of marine foods in the RB period is indicated by an increase in δ¹³C between the LIA and RB subadults. After early childhood, there was no age-dependent variation in dietary protein in the RB and LIA populations from Dorset. We propose that these changes related to the introduction of urban living, Romanised diets and population migration.     

Probabilistic models of seasonal <Emphasis Type="Italic">Bison</Emphasis> exploitation based on fetal prey osteometry and reproductive phenology

Seasonality studies are a staple of Great Plains archaeology, but obtaining evidence for seasonal food procurement is challenging. This study explores seasonality through the lens of fetal bison remains. A method is developed that produces intra-annual probability distributions for the date of fetal death. These distributions are informed by osteometric data from fetal bison in archaeological contexts, fetal skeletal growth curves, and data-driven models of bison conception dates. Probabilities can be assigned to seasonality hypotheses by examining date intervals across these intra-annual distributions. This method is implemented through an R program and applied to seasonality hypotheses at three archaeological sites containing fetal bison remains: Big Goose Creek (Wyoming), Baker Cave III (Idaho), and the Upper Tucker Site (Texas). Unlike previous efforts to infer seasonality from fetal bison remains, this method provides explicit probabilities showing the level of agreement between the osteometric data and seasonality hypotheses. This probabilistic approach could be extended to seasonality studies involving other animal taxa both within and outside of North America.     

Morphometric Analysis of Camelid Remains from the Alero Deodoro Roca (ADR) Site (Córdoba,Argentina). An Attempt to Characterise Body‐Size Variability in Sierras Pampeanas Australes Region

This paper focuses in the morphometric analyses of Lama guanicoe remains recovered from the hunter–gatherer archaeological site Alero Deodoro Roca, located at Sierras Pampeanas Australes region (Córdoba Argentina), an area that has little information on the body‐size variability of these artiodactyls. Because of the sparse osteometric data in the region, we focused on determining inter‐intraspecific differentiation of wild camelids through corroborated osteometric techniques and multivariate statistical analyses of proximal phalanges. Our results showed a clear interspecific differentiation between modern vicuñas and archaeological specimens. We could also distinguish at least three guanaco size groups (small, medium and large) with the archaeological remains from the late Holocene (3000–3600 bp ) of the site, presenting medium‐size characteristics. Finally this paper aims at establishing a first approach in the characterisation of guanaco body size in the mountainous region of Córdoba. Copyright © 2016 John Wiley & Sons, Ltd.     

Archaeological Amerindian and Eskimo cranioskeletal size variation along coastal western North America: relation to climate,the reconstructed diet high in marine animal foods,and demographic stress

A quantitative bioenvironmental study of cranioskeletal size has been made of a latitude-ordered sequence of 14 series of adult skeleton sets (N=305): the archaeological remains of aboriginal maritime peoples inhabiting the west coast of North America between southern California and the Arctic. Of eight osteometric parameters of cranioskeletal size developed, the most important are partial skeleton volume (PSK) and cranial capacity (CC); and the principal bone-affecting environmental factors are the northerly climate (CLIM2c), the reconstructed diet deficient in calcium and overabundant in animal foods and vitamin D (DIET3c), and demographic stress (DSg). Prior research has demonstrated that cranioskeletal size increases with geographical latitude and decreases under dietary calcioprivation and unphysiologically high birth rates. Upon linear multifactorial statistical analysis our main finding, limited to the Amerindian subsequence (n=11 series) from the Santa Barbara Channel, the San Francisco Bay Region and the Northwest Coast, is that PSK and CC vary in direct proportion to CLIM2c, and inversely with DIET3c and DSg. Controlling for the latter two osteoantitrophic factors, coastal Amerindian female PSK rises by 9% between 34 and 54°N latitude, CC by 5%; and the male incremental rates are somewhat higher, 13 and 10%, respectively. This newly discovered climate-dependent race cline, an example of Bergmann's Rule, is interpreted as factual evidence of a mid-Wisconsin or earlier arrival date for the first Palaeoindians. Cranioskeletal size of the very small (n=3 series) Western Eskimo subsequence approximates the northernmost values of the coastal Amerindian distribution. © 1998 John Wiley & Sons, Ltd.     

Zooarchaeological evidence for Moslem and Christian improvements of sheep and cattle in Portugal

This study of osteometric variation of sheep and cattle remains from archaeological sites in southern Portugal—the part that was once ruled by the Moslems—reveals an increase in size of the sheep in Moslem times and a subsequent increase of the cattle following the Christian conquest. It is assumed that a size increase reflects improvement. Sheep size increase is easy to understand given the Moslem fondness for mutton. The later size increase of the cattle is less easy to understand but could reflect a dietary switch from mutton to beef as well as the need for bovine power.     

Skeletal evidence for hyperparathyroidism in a 19th century child with rickets

The skeletal remains of a child aged 2.5–3.5 years, recovered during archaeological excavations at the churchyard of St Martin's Church, Birmingham, UK, were examined using gross observation, radiography and scanning electron microscopy. Lesions suggestive of the presence of rickets and of secondary hyperparathyroidism were found. This appears to be a first report of secondary hyperparathyroidism in response to rickets in a palaeopathological specimen. The potential of microscopic examination of bone for interpreting disease processes is emphasised. Copyright © 2006 John Wiley & Sons, Ltd.     

Chemical and strontium isotope analysis of Yaozhou celadon glaze

To produce useful information about the raw materials used in northern Chinese Yaozhou celadon glazes Chinese glazes (especially the source of the calcium‐bearing flux), Sr isotope analysis has been used for the first time. Yaozhou celadon is one of the most important representatives of northern Chinese greenware. The study has enhanced our understanding of the raw material sources used to make Yaozhou glazes dating from the Tang to Northern Song dynasties. It is highly likely that ‘Fuping stone’ mentioned in the historical record is not the main raw material used to make Yaozhou celadon glazes of the Tang to Northern Song dynasties. The results of ⁸⁷Sr/⁸⁶Sr analysis of the Yaozhou celadon glazes studied produce relatively consistent ⁸⁷Sr/⁸⁶Sr isotopic signatures, with a wide variation of relatively high Sr concentrations. This is firm evidence that a calcium‐bearing plant ash was the source of the calcium and of the flux in the Celedon glazes studied, and not, as some have suggested, limestone or ‘Liaojiang stone’. The study had illustrated that the present approach has great potential in providing a new way of reconstructing porcelain glaze technology.     

Evaluation and interpretation of residual rickets deformities in adults

Recent research has considerably improved the recognition and recording of rickets and osteomalacia in archaeological human bone, but the recognition and recording of residual deformities of rickets present in adults is still limited. The aim of this study was to assist with differential diagnosis of residual rickets deformities and determine which of the deformities that are characteristic of rickets could be identified in the adult skeleton. Long bone deformities were investigated, with research focusing on deformities of the leg bones. One hundred and thirty‐five adults from the historic cemetery of St Martin's, Birmingham, where vitamin D deficiency was known to have been widespread, were analysed. Deformities of leg bones were three times as frequent as arm bone deformities, and deformity of both the femur and tibia was the most frequently recorded combination of leg bones. Medial bending of the tibiae and anterior bending of femora, both at the proximal end, were the most frequently recorded types of deformities. A review of a range of conditions that can cause bowing deformities, and comparison of bowing recorded in children with rickets and the present cases, concluded that the most likely cause of the deformities recorded was residual changes linked to rickets. In this community, bowing deformities linked to rickets were quite common amongst adults and probably did not have adverse social consequences, but further studies are required to determine whether this attitude to deformity was more widespread. Copyright © 2008 John Wiley & Sons, Ltd.     

Osteometric and molecular sexing of cattle metapodia

Sex identification of skeletal remains based on morphology is a common practice in Zooarchaeology. Knowledge of the sex distribution of slaughtered or hunted animals may help in the interpretation of e.g. hunting or breeding strategies. Here we investigate and evaluate several osteometric criteria used to assess sex of cattle (Bos taurus) metapodia using molecular sex identification as a control of the metric data. The bone assemblage used to assess these new criteria derives from the Eketorp ringfort in the southern parts of Öland Island in Sweden. One hundred metapodia were selected for molecular analysis of sex and we were able to genetically identify the sex of 76 of these elements. The combined results of the molecular and osteometric analyses confirm a significant size difference between females and males for several measurements for both metacarpals (Mc) and metatarsals (Mt). Our results show that some measurements are applicable for metapodials. These measurements include the slenderness indices such as the Mennerich’s index 1 and 3, as well as the distal breadth (Bd), the breadth between the articular crests (Bcr), and the maximum breadth of the lateral trochlea (BFdl). We show that they can be used for sexing of both metacarpals and metatarsals. The latter measurements offer an opportunity to study fragmented elements and thus a higher number of elements may be utilized for morphological sexing of archaeological bones. Size comparisons of Mc and Mt may also aid in the separation of bulls and oxen.     

The Early Bronze Age settlement of Fidvár,Vráble (Slovakia): reconstructing prehistoric settlement patterns using portable XRF

This paper presents first results of chemical analyses of sediment samples from the Early Bronze Age (EBA) settlement Fidvár near Vráble (Slovakia). Large-scale geomagnetic prospection revealed detailed architectural remains of a fortified settlement. Other structures such as an earthwork of the linear pottery culture and at least two Roman march camps were found too. An Auger programme provided sediment samples of different depths, A) from an EBA house and the outer fortification ditch, B) from a potential metal workshop area as indicated by surface finds, and C) from an area located in the settlement's centre. The samples were analysed by a portable X-ray fluorescence (pXRF) spectrometer in order to investigate the vertical and horizontal distribution of chemical signatures. The comparison of geophysical data and three-dimensional chemical patterns gives us the opportunity to determine the human impact and to reconstruct activity patterns. The potentials and limits in the application of pXRF in archaeological soil chemical surveys are evaluated by systematic comparisons with high resolution laboratory measurements, including atomic absorption spectrometry (AAS) and inductively coupled plasma optical emission spectrometry (ICP-OES). The pXRF analyses can be reproduced well and show a good accuracy. Whilst (p)XRF analyses reflect the total composition of archaeological soil samples, the results of the AAS and ICP-OES analyses are fundamentally influenced by the acid digestion or extraction methods used. The pXRF analyses of the Vráble soils showed typical patterns of human occupation. For example, there are strong positive correlations between phosphorous, strontium and calcium. Phosphorous shows an enrichment in pathways and the fortification ditch. It is less strongly enriched within the sediments of house structures. Similar results apply to calcium and strontium distribution. But compared to the respective concentrations in sediment samples from the ditch they show a considerably higher variation in samples associated with house structures; that is, individual samples of sediments from house structures contained high concentrations of both, calcium and strontium. The deposits of an area that according to surface finds was thought to represent a potential metal workshop did not show indicative chemical signatures. Targeted excavations confirmed the absence of such as workshop. Finally it was shown that the refill history of the innermost fortification ditch (sample area D) and the overall cultural occupation sequence at the site correlate strongly with the chemical signatures of the respective ditch profile. The systematic variation of key elements along the profile opens up an entirely new perspective for interpreting the site's history. Overall, the study shows the great potential of pXRF as valuable part of an archaeological survey toolkit.     

Rickets in a High Social Class of Renaissance Italy: The Medici Children

Exploration of the Medici Chapels in the Basilica of San Lorenzo in Florence revealed the burials of nine juvenile members of the Medici family (16th–17th centuries). The estimated children's skeletal ages ranged from newborns to 5‐year‐olds, showing a series of bone abnormalities, in particular diffuse periosteal new bone and bowing of long bones. The comparable pathological lesions, including porosity evident on the skull, orbital roofs, costocondral ribs and growth plates between metaphyses and epiphyses, enlargement of metaphyses and sternal rib ends, and long bone bending, are interpreted as the skeletal manifestation of rickets. The diagnosis of a metabolic disease linked to vitamin D deficiency would appear to be unexpected for children brought up at the court of a Renaissance elite class family like the Medici of Florence. Analysis of the historical and social background is particularly helpful to understand the causes of the onset of the disease in this aristocratic group. Documentary sources, supported by ¹³C and ¹⁵N bone collagen analysis, attest that weaning of these children took place when they were around 2 years old. With a prolonged breast‐feeding and a delay in introducing solid food in the diet, vitamin D deficiency is expected to rise considerably, in particular if the other main risk factor, namely inadequate sunlight exposition, is associated with this human milk‐based diet. Copyright © 2013 John Wiley & Sons, Ltd.     

ON THE EMBALMMENT OF S. FRANCESCO CARACCIOLO

The Italian saint Francesco Caracciolo died in June 1608 and his body was transported from Agnone in the Abruzzo region to the city of Naples, where he was interred. According to written sources, his body was embalmed, but the embalmment method is unknown. In the present work, four samples of the remains of S. Caracciolo have been analysed for traces of embalmment. No organic compounds usually used for embalmment were found using GC with MS detection. Only low background levels of As were measured using CV‐AAS (Cold Vapour Atomic Absorption Spectroscopy). In two samples analysed by CV‐AAS, however, very high concentrations of Hg (12.7 μg g^?1 and 4.9 μg g^?1) were measured, these being 100 to 1000 times more than the background Hg levels found in bone tissue of other medieval and Renaissance individuals. Two interpretations seem viable: (1) the high Hg levels are caused by embalmment using Hg‐containing compounds; (2) the excess Hg originates from a medical treatment that S. Caracciolo received in his youth, when he was cured of leprosy, or in connection with his illness just prior to his death. The case of excess Hg in the remains of S. Caracciolo has distinct parallels with those of other Renaissance burials.     

Nitrogen isotopes and the trophic level of humans in archaeology

Human and domesticate animal bone collagen δ¹⁵N values in prehistory differ generally by 3‰ or more from Neolithic to post- Roman times in Northwest Europe, leading to an assumed dietary animal protein fraction of 60–80% using a standard interpretation of δ¹⁵N values. We examine the assumptions on which this model rests and the limitations of our knowledge in the analysis of δ¹⁵N values in archaeology. We have developed a set of models which, with small changes made in assumptions (on the order of 1‰), can produce substantially lower estimates of the dietary animal protein fraction for given δ¹⁵N values. We consider the implications of various dietary animal protein fractions on agricultural carrying capacities and human population densities in prehistory.     

Using ancient DNA identification and osteometric measures of archaeological Pacific salmon vertebrae for reconstructing salmon fisheries and site seasonality at Dionisio Point,British Columbia

Determining the diversity of salmon species represented in archaeological contexts is critical to understanding patterns of salmon harvesting and consumption, storage practices and seasonal patterns of resource exploitation by precontact peoples of the Northwest Coast. We present species determinations generated for salmon vertebrae from the Dionisio Point site (DgRv-003) in coastal southwestern British Columbia, a well-preserved village site that dates to between 1500 and 1300 cal BP. Molecular species identification was possible for 70 Pacific salmonid vertebral elements recovered from one excavated plankhouse at the site. Coupled with osteometric measures, we determine that residents of the plankhouse fished primarily for chum, sockeye and pink salmon, with a lesser emphasis on chinook, coho and steelhead. These results indicate a diverse fishery that likely occurred between July and December, suggesting a mid summer through winter season of occupation for the village.