URANIUM-SERIES DATING OF FOSSIL BONES FROM ALPINE CAVES

During the course of an investigation of fossil cave bear populations the uranium-series method for absolute age determination has been applied to bone material. The applicability of the method to bone samples from alpine caves could be demonstrated by the concordance of U/Th and U/Pa ages and cross-checks with the radiocarbon method. Stratigraphic agreement between bone ages and carbonate speleothetn ages also indicates the potential of the uranium-series method as a suitable tool for the age determination of fossil bones from alpine cave environments.     

Analysis of Neolithic human remains discovered in southern Italy

The authors describe the discovery of the remains of two unidentified skeletonised individuals in a small town located in southern Italy. The bodies were discovered while workers were preparing to lay an oil pipeline. The two individuals were found at a depth of 2 meters, and in very close proximity to one other. The recovery process of the skeletonised remains and their related findings, carried out by a team of forensic anthropologists and archaeologists, is described here.Archaeological examination determined that the remains date back to the 4th millennium B.C. Forensic anthropological and odontological examinations were performed to determine the biological profile of the skeletal remains by estimation of age and height, as well as the determination of sex. Age determination was performed by the Kerley and Ubelaker (1978, Revision in the microscopic method of estimating age at death in human cortical bone. Am. J. Phys. Anthropol., 49, 545–546) histological method using a 1 mm thick piece of bone tissue taken from the diaphyses of femur in both individuals. Dental age was estimated by examining root transparency. In order to determine the height of the individuals, various research methods based on the dimensional values of particular skeletal structures were applied. DNA analysis showed genotype differences of all the systems as compared to the haplotypes of present day subjects. This provided confirmation that the skeletal remains were from individuals of an ancient population (4th millennium B.C.). In addition, radiocarbon dating provided useful information as to the approximate period of death of the individuals. Interpretation was further enhanced by analysis of various bone fragments from each of the skeletons by high resolution mass spectrometry. 3D computerized imaging was used to analyse the patterns of skull fractures present, which resulted in supporting the hypothesis that the fractures were caused by stoning.     

Isotopic dietary reconstruction of humans from Middle Bronze Age Lerna,Argolid, Greece

This study presents the results of a carbon and nitrogen stable isotope analysis of 39 human bone and 8 animal samples from Middle Bronze Age (or Middle Helladic, MH, ca. 2100–1700 BC) Lerna, Greece. The isotopic data indicate that the humans had a C₃ terrestrial diet while certain individuals appear to have significant amounts of animal protein in their diet. With regard to weaning age, the isotopic values and the estimated age of early enamel disruptions suggest that solid foods were starting to be used as a substitute for breast milk at or before the ages of 2.5 and 3 years old.     

A NEW DIRECT RADIOCARBON AMS DATE FOR AN UPPER PALAEOLITHIC HUMAN BONE FROM SIBERIA

A new AMS radiocarbon date has been obtained from a Siberian human bone sample taken directly from a cranial fragment. The specimen comes from the north‐east Siberian site of Pokrovka 2 (55⁰19′46.30″N, 92⁰26′48.80″E). The remains consist of the anterior portion of the cranium or the superior part of the face; that is, the forehead and the roof of the eye sockets. Sadly, indifferent features of the frontal bone do not allow a confident diagnosis of the sex of the individual. The evidence suggests, however, that the remains are probably those of a young adult (teenager) and an anatomically modern human, rather than a Neanderthal. Radiocarbon dating was undertaken at the Oxford Radiocarbon Accelerator Unit (ORAU). The radiocarbon age determination of the bone fragment, 27 740 ± 150 bp (OxA‐19850), is one of the earliest direct dates for a modern human from Siberia. The Pokrovka cranial fragment dates to the middle Upper Palaeolithic of Eurasia and is broadly contemporary with the human remains from the famous eastern European site of Sunghir. When the radiocarbon age is compared with the Cariaco Basin ¹⁴C data set and the Greenland oxygen isotope record of NGRIP, it corresponds with Greenland Interstadial 5, a warmer phase of the Last Glacial period, although this is a preliminary conclusion, and is based on climate records that may or may not be wholly synchronous between the two locations.     

Intrataxonomic variability in metapodial and femur bone density related to age in guanaco (Lama guanicoe). Zooarchaeological and taphonomical implications

The aim of this paper is to present new information about bone mineral density (BMD) variability in guanaco (Lama guanicoe), a South American wild camelid; while taking into account its ontogenic development. The importance of considering intrataxonomic variability in bone density related to age for constructing zooarchaeological indices and applying them to archaeological assemblages is also discussed. The results presented here were developed from a large sample of 46 metacarpals and 45 femurs of modern guanacos, ranging in age from ca. 6 months of gestation to 12 years. Bone structural densities were measured using a biphotonic X-ray densitometer, DEXA method, Norland EXCELL and the bone mineral content (BMC) of each scanned portion or complete element was divided by its volume, which was obtained independently by water displacement. Our results indicate that the bone density of each anatomical unit is highly variable, even among individuals of the same species. According to metacarpals data, age is an important factor affecting BMD. Moreover, our study shows that the density ranges of different skeletal elements can overlap, suggesting that it is not possible to predict that a certain element is always denser than another one. Finally, we present new evidence about the potential age-related taphonomic bias in faunal assemblages, indicating that meanwhile some skeletal elements show clear differences in their density between their fused and unfused state (metacarpal) other do not (femur).     

Possible evidence of mammoth hunting during the Epigravettian at Yudinovo, Russian Plain

Whether or not mammoth hunting was practised during the Late Palaeolithic has been a controversial issue ever since large accumulations of woolly mammoth bones associated with prehistoric artefacts were discovered more than 100 years ago. Detailed taphonomic and palaeobiological analyses of the mammoth bone complexes from the Epigravettian Yudinovo site in the Russian Plain were carried out. The combination of the homogeneous weathering rate of the mammoth bones, the isolated state of most of the skeletal elements, the restricted spatial range of the carnivore gnawing traces, the breakage pattern of the skulls and long bones, the sex ratio, the small body size of the adult mammoths, the age profile (with an important frequency of prime-aged cows), and the large number of individuals, suggest that the bone complexes at Yudinovo were constructed from body parts and bones that were extracted from freshly killed mammoths and that mammoth hunting was practised at this site during the Epigravettian.     

The long–term survival of bone: the role of bioerosion

Fossil bones (N = 350) spanning more than 350 million years, and covering a wide range of depositional environments, were studied to compare the distribution of microbial destruction features in fossil bones with previously published data sets of bones of archaeological age. The distribution of bioerosion in fossil bones is very different from that found in bone from archaeological sites. Fossil bones typically show little or no bioerosion. Under normal conditions, if a bone is to survive into the fossil record, then rapid bioerosion must be prevented (or halted). This conclusion suggests that early post mortem processes,such as the mode of death, influence the potential of any bone to survive into deep time.     

Age‐Related Cortical Bone Maintenance and Loss in an Imperial Roman Population

This article explores age‐ and sex‐related patterns of cortical bone loss, assessed by radiogrammetry of the second metacarpal, from a skeletal sample excavated from the Imperial Roman port city of Velia (1st and 2nd century ad ), to contribute to our understanding of health and disease during the Imperial period. Patterns of age‐ and sex‐related bone loss and health at Velia are also compared with other historical samples and to modern clinical data. It was hypothesised that patterns of age‐ and sex‐related bone loss in this well‐represented Roman sample (n = 71) would mirror those reported in modern populations. Differences in the quantity of cortical bone between three age groups (18–29, 30–49 and 50+ years) are significant in both men and women; however, the pattern of bone loss differs between the sexes. Women show a gradual decline in bone quantity beginning in middle age, whereas men only lose bone in old age. However, there are no significant sex differences at any age group in the metacarpal cortical index. These findings suggest the hypothesis should be refuted because significant sex differences, particularly in old age, are an expected hallmark of bone loss in Western populations today. The possible biocultural and gender‐related factors that may have contributed to the observed patterns of bone maintenance and loss at Velia are discussed. Copyright © 2011 John Wiley & Sons, Ltd.     

Laser ablation depth profiling of U-series and Sr isotopes in human fossils

We have explored laser depth profiling to obtain data sets for U-series as well as Sr analyses. Laser probing with an 81 μm spot size allows for the exploration of low uranium domains of up to 400 μm below the outer surface in tooth enamel. These low U domains will contain Sr isotope compositions of the individual, that are least affected by diagenetic Sr overprints. The small holes drilled for U surveying are not visible to the naked eye. Using larger spot sizes of around 233 μm, laser drilling can be used to obtain reliable U-series isotope data to a depth of approximately 1000 μm in enamel and around 1300 μm in bone. Furthermore, meaningful ⁸⁷Sr/⁸⁶Sr isotope data can also be obtained with this spot size. Using our sampling strategy, the overall damage to a human tooth is minute, as demonstrated on a Neanderthal tooth from Moula-Guercy. We expect that laser ablation depth profiling will become routine for gaining insights into the age of human fossils and the migrations of ancient humans.     

The periodontal disease status of the historical population of Assos

The science of anthropology obtains data on health, disease and death from ancient populations. Research on the skeletal remains of human teeth and surrounding tissues provides useful information on the evolutionary perspective of dental and periodontal diseases. The aim of this study was to determine the prevalence and severity of periodontal diseases in the skeletal remains of 33 Assos inhabitants of the 4th century BC. Three age groups were constituted for the studied population. Periodontal disease status was determined based on the textural and architectural variations of the interdental septum and the extent of bone loss. It was found that the prevalence of periodontal diseases increased with age. The severity of periodontal bone loss also increased with age and the mean alveolar bone loss between age groups was statistically significant (P = 0.004). This analysis of the dental health of ancient populations provides insights into the aetiology, patterns and distribution of periodontitis, which has a very complex disease pathogenesis. Copyright © 2005 John Wiley & Sons, Ltd.     

The Use of Multiple Correspondence Analysis (MCA) in Taphonomy: The Case of Middle Helladic Asine,Greece

The goal of this paper is to investigate whether multiple correspondence analysis (MCA), a multivariate statistical technique, is a useful dimensionality‐reduction tool in zooarchaeological and taphonomic studies. For this purpose, the focus is to detect and discuss traces of waste management. Animal bones from waste‐related contexts at the Bronze Age site Asine, Greece, are investigated. The data consist of bone fragments dating to the Middle Helladic from this site. Unidentified fragments were categorised in size classes, where possible. Information on taxa, skeletal parts and the presence or absence of several taphonomic markers is included in the data set. The MCA reveals several correlations of zooarchaeological interest. For example, the association between indeterminate fragments and calcined bone points to issues concerning identification and preservation. Floors are characterised by weathered long‐bone fragments from medium‐sized mammals. Additionally, the results of MCA indicate that the material might have suffered from density‐mediated attrition, based on the abundance of axial fragments, which did not differ between different contexts and taxa. The results show that MCA can be used to detect zooarchaeological and taphonomic patterns. This multivariate technique is useful when investigating large data sets, as is often the case with large zooarchaeological assemblages. Copyright © 2016 John Wiley & Sons, Ltd.     

Prehistoric hunting pressure estimated by the age composition of excavated sika deer (Cervus Nippon) using the annual layer of tooth cement

Age of excavated sika deer was determined not only by the cement-annuli method but also by the observation method to avoid unnecessary destruction of archaeological materials. The observation method is based on tooth replacement, wear index and the appearance of cervical lines on the molar teeth. Stepwise discriminant analysis was used to establish a criterion for the age determination of excavated mandibles. Then the annulation method was carried out for well-preserved specimens older than 3–5 years. The age composition of excavated deer mandibles seems to have changed through the Jomon Period. Age composition at the Torihama sites (the Early Jomon Period) was characterized by an abundance of aged individuals, showing a close similarity with that of the recent deer population under protected conditions. Deer from the Kidosaku and Yahagi sites (the Late Jomon Period) and the SambuUbayama site (the Latest Jomon Period) were composed mainly of deer younger than 5 years, which seems to correspond to that of the recent hunted population living under natural conditions in Hokkaido. These data suggest that the hunting pressure increased during the Late and Latest Jomon Periods and compares with the pressures on recent hunted populations of sika deer.     

On the Use of Endosteal Layers and Medullary Bone from Domestic Fowl in Archaeozoological Studies

The very high incidence of medullary bone in two archaeozoological assemblages of the Roman period was believed to reflect systematic slaughtering of older hens at the end of the egg laying season. In an attempt to test this hypothesis, histological analyses were carried out. Histological data in the literature on ageing of modern fowl and on the development of medullary bone in hens are insufficient for application to archaeozoological material. Bones of modern fowl of known age were analysed with the aim of validating the use of endosteal layers for ageing. In addition, hens with known egg laying stage were studied in order to try and document differences in medullary bone development that could be related to the time of slaughtering (just before, during, or just after the egg laying season).     

Fusion status of the jugular growth plate: An aid for age at death determination

To assess the feasibility of the fusion (synostosis) status of the jugular growth plate as an age at death indicator, a series of 98 skulls of documented age and sex from the so-called ‘Utrecht collection’ was examined. Ages at death in the series ranged from 14 to 92 years. Only specimens that could be inspected bilaterally were taken into account. According to the few but conflicting data from the literature, fusion happens at 16–18 years, or starts at ca. 25 years of age. In our sample no fusions were seen before the age of 22 years. Unilateral fusions were found only in the age range 22–34 years. At ages above 34 years in females and above 36 years in males all jugular growth plates were fused bilaterally. These results indicate that examination of the fusion status of the jugular growth plate is of use in age at death determination.     

Micro-sampling of human bones for mobility studies: diagenetic impacts and potentials for elemental and isotopic research

The nature of long bone formation and the pathways of interaction between bone samples and the burial environment suggest that portions of the bones disconnected from the arterial system are resistant to diagenetic alteration. Preliminary work on femurs from Early Bronze Age hunter-gatherers in Cis-Baikal, Siberia shows that the nature and progression of chemical changes in the bone matrix due to microbial attack can be analyzed using laser ablation inductively coupled plasma mass spectrometry. Intra-osteon variability in elemental concentrations and strontium isotope ratios (⁸⁷Sr/⁸⁶Sr) indicate the presence of unaltered portions of bone within diagenetically modified bone and suggest that useful data remain accessible. These biogenic signals can potentially be useful for mobility research in broad terms and the smaller timescales within an individual's lifetime (months, years), accessible therein. Laser ablation micro-sampling of femur specimens showed that intra-osteon elemental composition of Ba, Re, and Cs varied within and was correlated between multiple osteons of a single bone. Portions of chemically unaffected bone were identified within, and effectively discriminated from diagenetically altered bone tissue. Areas showing visual alterations and erratic or uncorrelated Ca and Sr elemental results also had anomalous Sr isotope ratios, suggesting diagenetic alteration in those places. Compositional and isotopic analysis of intact portions of bone supports the hypothesis that hunter-gatherer groups in Cis-Baikal made numerous major movements during their lives. Microscopic analysis of long bones clarifies aspects of biodeterioration and correlations between trace elemental results and diagenetic alteration. Micro-sampling of intact portions of bone expands the scope of available materials for research on mobility and other aspects of human past behavior.     

Evaluating human skeletal growth: An Anglo-Saxon example

Cross-sectional growth data were obtained from the skeletal remains of non-adults from the Raunds Anglo-Saxon site. Standard measurements of the diaphyseal lengths of the long bones of the upper and lower limbs and the maximum breadth of the ilium were recorded in order to construct skeletal growth profiles (SGP). In addition regression equations were used to estimate diaphyseal length from proximal and distal shaft widths, and epiphyseal breadth data for fragmentary remains. The skeletal measurements were then plotted against age estimates determined by the dental formation standards of Moorrees, Fanning and Hunt, and Anderson, Thompson and Popovich. The growth data were compared with sixth to seventh century German, ninth century Slavic and modern Caucasian data. With the exception of the ancient Slavic material, the Anglo-Saxon remains demonstrated the smallest rates of growth. Diaphyseal ageing curves derived from the Anglo-Saxon sample were tested for applicability on the non-adult cohorts of the Berinsfield and Exeter Anglo-Saxon/Early Medieval samples. Differences were observed between diaphyseal age as determined from the skeletal growth profiles for Raunds and calcification age assessed for individuals within the test samples. It is proposed that variation in long bone growth as well as dental age confounds consistent and reliable ageing of skeletal remains based on diaphyseal length. Assessment of changes in health and evaluation of methodological problems inherent to studies of skeletal growth from archaeological populations are discussed. Population comparisons for changes in general health are recommended over individual assessments.     

THE DESIGN OF EVALUATION OF ASSAYS OF TRACE ELEMENTS IN A FOSSIL BONE*

The flame atomic absorption spectroscopy method of analysis of trace elements (Mn, Sr, and Cu) in a mammoth's bone and the method of estimation of the data using regression analysis are described. The concentration fields of the single elements in a cross-section through the bone are well described by a fourth degree polynomial. The minimum values and their coordinates have been determined. A method of determination of the original concentrations of trace elements in fossil bones is described further. The approximating features of the regression exponential equations and of the diffusion functions of various shapes were determined and a method for calculating the concentrations and diffusion properties of the bones once deposited in the soil is suggested. The original trace element concentration can be estimated by extrapolation of a suitable exponential or diffusion function.     

Sexual dimorphism in limb bones of ibex (Capra ibex L.): mixture analysis applied to modern and fossil data

Estimating sex ratios of fossil bone assemblages is an important step in the determination of demographic profiles, which are essential for understanding the palaeobiology and palaeoethology of any particular species, as well as its exploitation patterns by humans. This is especially true for ibex (Capra ibex), which was a main source of food for hominids during Pleistocene times. Classical methods for determining sexual dimorphism and sex ratio, such as analyses using uni‐ and bivariate plots, are based on an arbitrary fixing of limits between sexes. Here we use a more robust statistical method termed mixture analysis (MA) to determine the sex of postcranial remains (long bones, metapodials and tarsals) from ibex. For the first time, we apply MA to both a modern and a fossil sample of one species, by using metric data taken from (i) a collection of present‐day ibex skeletons and (ii) a Palaeolithic sample of the same species. Our results clearly show that the forelimb (humerus and radius) is more dimorphic than the hindlimb (femur and tibia) and is therefore better suited for sexing ibex. It also appears that metapodials should be used carefully for estimating sex ratios. On the basis of these results, we propose a classification of bone measurements that are more or less reliable for sexing ibex. The results of MA applied to the ibex fossil bones from the Upper Palaeolithic site of the Observatoire (Monaco) lead us to the conclusion that this assemblage consists of a majority of males. The quantitative estimations calculated by the MA make it possible to compare the size of Pleistocene and modern ibex for the whole set of variables used in this study. Copyright © 2006 John Wiley & Sons, Ltd.     

Interpreting Urban Food Supply and Distribution Systems from Faunal Assemblages: An Example from Colonial Massachusetts

Animal bone assemblages from four historical sites in eastern Massachusetts are analysed in a comparative framework to interpret aspects of urban food systems. Characteristics of the urban environment and the nature of food supply and exchange systems are interpreted from taxonomic representation, body part representation, butchery patterns, and age and seasonal slaughtering patterns. These analyses show that specialized husbandry to supply urban markets was limited during the seventeenth and eighteenth centuries, and that many aspects of urban food distribution systems followed traditional rural patterns. A model that explores the relation between patterning of bone collections and characteristics of urban areas is applicable to other studies of urban assemblages. © 1997 by John Wiley & Sons, Ltd.     

The Middle Byzantine menu: palaeodietary information from isotopic analysis of humans and fauna from Kastella,Crete

We report here on the measurements of carbon and nitrogen stable isotopes of bone collagen from the Middle Byzantine site of Kastella, in the city of Heraklion, on the island of Crete, Greece. The data derived from the analysis suggest a diet based primarily on terrestrial, C₃ protein, probably from animal sources, with the inclusion of some marine protein. The adult diet at this site is relatively uniform, with no detectable differences between average isotopic values for males and females. We also found that bone collagen δ¹⁵N values for a small number of juveniles decreased to adult levels after the age of two years, indicating that weaning occurred at or before this age. Copyright © 2006 John Wiley & Sons, Ltd.