Stable isotope analysis of humans from Xiaojingshan site: implications for understanding the origin of millet agriculture in China

Millet agriculture originated in Northern China in the early Neolithic period (ca. 8000 BP), however, the actual importance of millet in human diets is still not clear. To determine the relative contribution of millet in human diets in this period we undertook stable isotope analysis of humans from Xiaojingshan site and fauna from Yuezhuang site, both of which are attributed to the Houli Culture and date to about 8000 years ago. The carbon isotope values of human bone collagen showed that millet (as a C₄ plant) only contributed approximately 25% of dietary protein, with the rest from C₃ based plant and animal sources, if a simple mixing model is used. We did not observe any statistical dietary difference between males and females at the site, although it has been argued that the Houli Culture was a matriarchal society. Finally, we compared our data with other published isotopic data from the contemporary Jiahu site and Xinglongwa site and a number of sites from the subsequent Yangshao Culture and found that millet only became a significant source of dietary protein approximately 1000 years later, as human carbon isotope values from these later sites indicated that almost all of dietary protein came from C₄ (i.e. millet) sources.     

Stable isotopic analysis of human and faunal remains from the Incipient Chulmun (Neolithic) shell midden site of Ando Island,Korea

We analyzed stable carbon and nitrogen isotope ratios of human and animal remains from the Ando shell midden, South Korea. The Ando site is a rare Incipient Chulmun (Neolithic) site (ca. 6000–5000 BC), which contains well-preserved human and animal bones in shell mounds. The stable isotope results for humans (average δ¹³C = −13.5 ± 0.5‰ and δ¹⁵N = 15.2 ± 0.5‰) indicate that Ando people in the Incipient Chulmun period strongly depended on marine resources. There were no isotopic differences between humans of different sex and age at this site. We compared our data with other previous published isotopic data from the Chulmun sites and found that the Ando people had similar isotope values to the southern Chulmun people (Tongsamdong and Daepo), but different isotopic ratios than the western Chulmun people (Daejukri and Konamri). These results indicate that marine foods were the main food resources in the southern coastal regions, but not in the western coastal regions in Chulmun Korea.     

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Fifty-eight dental calculus samples from medieval and post-medieval skeletons from Vitoria, Spain, and a single sample from an Alaskan Inuit were tested for stable carbon and nitrogen isotope compositions. There was sufficient carbon and nitrogen concentrations to obtain δ¹³C and δ¹⁵N values, and the samples from Spain produced results that were replicable and comparable to European isotope values based on bone collagen collected from literature sources. The Alaskan Inuit calculus sample yielded a δ¹⁵N value of +17.5‰, well beyond the range of the Spanish samples, but consistent with literature data for modern Greenlandic Inuit consuming a diet rich in marine food. There are several potential sources for carbon and nitrogen in calculus. The results of this study yield stable isotope values consistent with those obtained from other biomaterials used as isotope proxies for paleodietary research, including bone collagen, hair, and fingernails, although further work is necessary to verify the fidelity of calculus as an isotope proxy. Many studies in bioarchaeology are precluded by curatorial concerns regarding the destructive analysis of primary biomaterials. However, calculus is an “add-on”, or secondary biomaterial, that is not an integral part of the dental or skeletal system. Hence, its consumption during analysis is technically not destructive. Therefore, isotope analysis of dental calculus may provide a potential new avenue for paleodietary analysis where the use of other primary biomaterials is precluded.     

Stable isotopic evidence of diet in a Greek colonial population from the Black Sea

Archaeological and literary sources indicate that the ancient Greeks relied heavily on terrestrial resources and that their access to certain types of foods varied by sex and status. Human and faunal remains from the Greek colonial site of Apollonia (5th to 2nd century BC) on the Black Sea coast of Bulgaria were analyzed for stable carbon and nitrogen isotopes in order to reconstruct the diet of this population, investigate the relative importance of marine vs. terrestrial resources, and explore variations in diet with respect to age, sex, and burial type. The results of this study indicate that the colonists of Apollonia relied on a mixed diet of terrestrial and marine foods, and that there was little or no variation in diet by age, sex, or burial type. The relationship between δ¹³C and δ¹⁵N data for these samples suggests that while marine foods were an important source of nitrogen, much of the carbon used to construct amino acids came from terrestrial foods.     

Stable carbon isotope analysis of human tooth enamel from the Bronze Age cemetery of Ya'amoun in Northern Jordan

Seventy-five teeth from the Bronze Age cemetery of Ya'amoun were sampled for carbon isotope analysis. The sampled teeth represented the transitional period of the Middle/Late Bronze Age. The results showed that the components of the diet that are reflected in the carbon isotope data remained the same during this period with an average δ¹³C of about −11.87‰. This value reflects more C₃ plant consumption.     

Late Stone Age subsistence in the Tilemsi Valley, Mali: Stable isotope analysis of human and animal remains from the site of Karkarichinkat Nord (KN05) and Karkarichinkat Sud (KS05)

The pathways leading to the adoption of cereal cultivation and pastoralism in West Africa are poorly understood. In order to elucidate the transition to food production during the Late Stone Age in Mali’s Tilemsi Valley samples of ancient and modern human and animal remains were selected for carbon and oxygen isotope analysis. Our results indicate the inhabitants of Karkarichinkat Nord (KN05) consumed considerable quantities (85%) of carbon derived from C₄ plants, either directly in the form of C₄ grasses such as wild Panicum sp. and possibly domestic Pennisetum sp. or indirectly through the consumption of C₄ grazers such as Bos sp. and Ovis sp.     

Stable carbon- and nitrogen-isotope ratios and electron spin resonance (ESR) g-values of charred bones: changes with heating and a critical evaluation of the utility of g-values for reconstructing thermal history and original isotope ratios

Changes in the stable carbon and nitrogen isotope ratios of modern bone samples heated to a variety of times and temperatures were used to determine the effect of heating on isotope ratios and the retention of organic matter in charred bones. For organic extracts produced by slow demineralization in weak acid, δ¹³C values were unchanged, while δ¹⁵N values increased by up to 5‰ and were primarily determined by heating temperature. Changes in the electron spin resonance (ESR) g-value of whole bone and organic extracts were also measured. For organic extracts from charred bones, the g-value was well-correlated with δ¹⁵N and temperature, suggesting that g-values could be used to estimate the charring temperature and original δ¹⁵N values of charred bones. Thus, g-values from demineralized extracts could be very useful in forensic investigations where it would important to reconstruct the thermal history of burned bones. Isotope ratios and g-values of demineralized extracts from four prehistoric components at three sites that produced cremated human bone were used to test whether the same approach can be applied to archaeological materials. While carbon isotope ratios of the prehistoric samples were similar to those of uncharred specimens, nitrogen isotope ratios were increased and the g-value corrections for nitrogen isotope ratios were not effective.     

Stable isotopic evidence for diet in a Roman and Late Roman population from Leptiminus,Tunisia

This study investigates the diet of the Roman and Late Roman population of Leptiminus on the Mediterranean coast of Tunisia. Stable carbon and nitrogen isotope analysis of human bone collagen and carbonate samples obtained from individuals buried in four cemeteries at Leptiminus was conducted in order to reconstruct the diet of the population, investigate the relative importance of marine vs. terrestrial resources, explore sex-, age-, and status-based variations in diet, examine temporal changes in the types of foods consumed, and compare the diet at Leptiminus with that of other Roman populations. The results of this study indicate that the residents of Leptiminus consumed a diet that was heavily reliant on terrestrial plant resources with the addition of a significant amount of marine resources. There were no significant sex differences in isotope values. In contrast, distinct dietary differences were seen between the adults and children. Nitrogen isotope values suggest that weaning began before the age of two and was completed by about 3 years of age, a finding consistent with previous isotopic studies of Roman samples. A temporal shift in diet is suggested by the nitrogen isotope values measured in samples from the most recent cemetery. A comparison of the data from Leptiminus with that derived from other Roman sites indicates that regional variability in diet existed within the Empire.     

Archaeobotanical and GIS-based approaches to prehistoric agriculture in the upper Ying valley,Henan, China

Archaeobotanical survey has sampled a series of late Neolithic to early Bronze Age settlements in the upper Ying valley (part of the central plain of China) and provided useful data for understanding prehistoric arable ecology and farming during a period of increasing local social complexity. A combination of the modelling functions offered by Geographic Information Systems (GIS) and the data reduction possibilities offered by Principal Component Analysis (PCA) allow us to explore possible relationships between local arable ecology, crop-processing strategies and the natural environment. The results should be treated cautiously given the size of the analytical sample but suggest that differences in the natural environment around each site may explain varying patterns of wild food collection, while social and cultural factors may better explain variation in farming practice and crop-processing at different sites.     

Applying new approaches to modeling diet and status: isotopic evidence for commoner resiliency and elite variability in the Classic Maya lowlands

Classic Maya states were characterized by a high degree of socioeconomic stratification. This paper investigates the degree to which status, as defined by grave goods and tomb construction, influenced dietary patterns of elites and commoners throughout the Classic Period (200–900/1000 AD) of the southern lowlands. We compile a database (N = 102) of previously-published stable isotope ratios (δ¹³C collagen, δ¹³C apatite, and δ¹⁵N collagen) from Maya bone mineral and collagen, and interrogate these data through two new isotopic modeling techniques: a simple carbon isotope model ( Kellner and Schoeninger, 2007; Froehle et al., 2010) and a multivariate isotope model ( Froehle et al., 2012). We find that Maya elite diet varied significantly through time in terms of maize consumption and trophic level, while commoner diet remained remarkably stable. These findings provide new information relevant to studies of ancient Maya class structure and to studies of subsistence strategies of the pre-Columbian Americas.     

Early Neolithic diets at Baijia,Wei River valley,China: stable carbon and nitrogen isotope analysis of human and faunal remains

Stable carbon and nitrogen isotope values are presented for faunal and human bone collagen from Baijia, in the Wei River valley region of Shaanxi Province, China. The remains have a calibrated age range of ca. 5709–5389 BC, and correspond with the early Neolithic Laoguantai Period. Stable isotopic results indicate that human diets included millet and probably aquatic foods such as fish and shellfish. Bovid samples are tentatively identified as water buffalo, and have a mean δ¹³C value of −14.6‰, which reflects some millet consumption. Whether bovids were grazing on wild millet, or had diets directly influenced by humans, is not known. The single Sus sample from Baijia had a diet dominated by C3 plants and is thus unlikely to have been a domesticated animal. Overall, the stable isotope results presented here conform to the current concept that the people of the Laoguantai culture were millet farmers, who had subsistence strategies that included hunted wild foods.     

Stable isotope evidence of human diet at the Nukdo shell midden site,South Korea

Carbon and nitrogen stable isotope analysis was undertaken on bone collagen extracted from archaeological human (n = 48) and animal (n = 45) skeletons from the Nukdo site, Location I C, South Korea. This shell midden and grave site is dated from the late Mumun (550–300 BC) to early Iron Age (300 BC-1 AD) periods. The herbivorous mammals fell within the range of C₃ consumers, with average values of δ¹³C = −21.0 ± 0.5‰ and δ¹⁵N = 3.6 ± 0.5‰ for deer (n = 16) and δ¹³C = −20.6 ± 0.5‰ and δ¹⁵N = 4.5 ± 2.0‰ for wild boar (n = 17). Humans from this site averaged δ¹³C = −18.3 ± 0.4‰ and δ¹⁵N = 11.2 ± 0.7‰ for adults (n = 15) and δ¹³C = −18.7 ± 0.7‰ and δ¹⁵N = 12.5 ± 1.1‰ for juveniles (n = 33). These δ¹³C values indicate that there was no significant input of C₄ plants in the human diets and this may be associated with the spread of rice agriculture in the Mumun period. Human bone collagen δ¹³C and δ¹⁵N values indicate that there was some consumption of marine foods, although the main protein sources were from terrestrial foods. The isotope data demonstrate that the humans at Nukdo had mixed diets that included marine and terrestrial protein, including C₃ plants such as rice. Finally, the isotope results from the juveniles indicate that weaning occurred before the age of 1.5 years in this period.     

Prehistoric Fijian diet and subsistence: integration of faunal, ethnographic, and stable isotopic evidence from the Lau Island Group

We explore subsistence practices and dietary change on islands in the central Lau Group of Fiji with zooarchaeological methods and stable isotopic analysis of human and animal skeletal material interpreted through an ethnoarchaeological lens. Our dataset combines detailed identifications of fauna, especially fishes, with stable isotopic (δ¹³C, δ¹⁵N) values of human and animal bone collagen and apatite carbonate spanning approximately three thousand years of human occupation on three study islands. Additionally, over fifty inshore and offshore contemporary fishing expeditions were observed on all study islands over a six-year period. We integrate these separate lines of evidence to form robust interpretations of Lauan subsistence patterns over a broad temporal scale. We add to the existing literature on stable isotopic analysis of archaeological bone from Remote Oceania and compare all of these groups through time. Our results indicate that Lauans differentially relied on nearshore reef resources rather than pelagic fishes, and terrestrial endemic species may have served as a portion of the diet during the early prehistoric period. Root crops (e.g., taro, yam) provided the majority of calories to the diet; however, sea grapes likely contributed to the early diet. Our isotopic results differ from previous studies of Fijian diet and that of Remote Oceania at a time of probable marine ecosystem shifts (AD 1300) illustrating diet breadth and variability in subsistence strategies potentially due to climatically influenced resource depletion.     

Alkaline cooking and stable isotope tissue-diet spacing in swine: archaeological implications

In this study we examine the effects of alkaline cooking on carbon and oxygen stable isotopic ratios of mineralized tissues from nine pigs raised on monotonous mixed C3/C4 vegetarian diets. Two sources of collagen (humerus and mandible) and two sources of apatite (humerus and enamel) were analyzed. Within each diet group, humerus and mandible collagens were found to record equivalent δ¹³C and δ¹⁸O ratios; however, enamel apatite was found to be enriched over bone apatite by 2.3‰ in carbon and 1.7‰ in oxygen. Alkaline cooking was found to slightly, but significantly increase the Δ¹³C_{collagen-diet} and Δ¹⁸O_{collagen-diet} of bone collagen. A similar trend towards enrichment was observed in bone and enamel Δ¹³C_apatite-diet and Δ¹⁸O _apatite-diet, but the differences were not significant. Observed isotopic shifts were consistent with increased nutrient utilization of the alkaline-cooked maize as compared to raw maize. In addition, a reexamination of the relationship between diet and tissue carbon isotopic values suggests that species and alimentary type should be considered when interpreting ancient diets.     

Paleodiet of domestic turkey,Shields Pueblo (5MT3807), Colorado: isotopic analysis and its implications for care of a household domesticate

Isotopic analysis of domestic turkey (Meleagris gallopavo) bones from Shields Pueblo, southwest Colorado, USA, suggests that these birds consumed a diet high in C4 plants. This contrasts with the diet of local herbivores, where much lower percentages of C4 plants were recorded. In view of the prevalence of maize (Zea mays) in the human diet of Ancestral Puebloan people, we suggest that turkeys were fed food scraps and surplus maize, rather than being allowed to forage for themselves. This suggests that turkeys were carefully tended in the household. Analysis of specimens from other sites in the northern Southwest shows that this pattern of turkey feeding characterizes all of the sampled horticultural communities.     

Stable isotope evidence for salt-marsh grazing in the Bronze Age Severn Estuary,UK: implications for palaeodietary analysis at coastal sites

Stable isotope analyses of modern coastal and salt-marsh plant species (‘salt-loving’ plants or halophytes) have demonstrated that these are significantly enriched in ¹⁵N compared to other terrestrial plants. Coastal salt-marshes were far more extensive in the past than they are today. They represented a vast and much-exploited resource in many areas of the UK and north-western Europe and were considered to be prime land for the grazing of animal stock.     

Reassessing the diet of Upper Palaeolithic humans from Gough's Cave and Sun Hole,Cheddar Gorge,Somerset, UK

Richards et al. (2000) reconstructed the diet of the human remains found in Gough's and Sun Hole Cave through isotope analysis. They concluded that these people consumed an entirely terrestrial-based diet. Their reconstruction was based upon comparison of the results from human bones with those from a very small number of associated animals. The diets of the Gough's and Sun Hole Cave human were different from the other six Upper Palaeolithic humans from the British Isles for which dietary information has been obtained through isotope analysis. The work of Richards et al. (2000) suggests that they were the only ones for whom marine or freshwater resources did not play a significant role in their diets. We test this through further analyses of faunal remains from Gough's Cave, Sun Hole and other contemporary sites (Kent's Cavern, Aveline's Hole, Kendrick's Cave). Despite the limited faunal sample, the original palaeodietary reconstruction is broadly consistent with our findings. The isotope values of the main protein sources consumed by the humans from both sites are consistent with those of red deer and bovines, and, for a single individual, with that of horse and red deer. Reindeer was postulated in the original reconstruction as a potential food source, but this seems very unlikely based on our isotope reconstruction and the archaeological remains.     

Stable isotope investigations of charred barley (Hordeum vulgare) and wheat (Triticum spelta) grains from Danebury Hillfort: implications for palaeodietary reconstructions

Palaeodietary studies typically focus on the analysis of bone collagen due to the limited availability of plant remains. Isotopic analysis of plant remains, however, allow for a more extensive consideration of the contribution of plants to the human diet and can potentially provide information about the environment in which the crops were grown. This paper reports the results of carbon and nitrogen isotope analyses performed on charred barley and wheat grains recovered from pits within Danebury Iron Age hillfort. To the best of our knowledge, this is the first Iron Age site in Britain from which charred grains have been isotopically analysed. Our results suggest that cereals found at the hillfort were grown in several different environmental contexts. The isotope data demonstrate that the herbivores were not consuming a diet primarily based on grains as the δ¹⁵N values of the grains are very similar to those of the herbivores. Palaeodietary investigations typically assume that humans eating plant protein only would have the same δ¹⁵N value as the local herbivores. This assumption is clearly invalid at Danebury, where the humans and animals appear to have consumed either different parts of the same plants or different plants. Researchers typically interpret high differences between human and animal δ¹⁵N values as indicative of diets high in animal protein, however where major plant resources have δ¹⁵N values similar to those of the herbivores our ability to distinguish between plant and animal sources of protein in the diet is limited. Our research has demonstrated that whenever possible it is desirable to measure the isotopic signatures of potential major plant resources in order to understand past subsistence strategies.     

Stable isotope analysis of Late Upper Palaeolithic human and faunal remains from Grotta del Romito (Cosenza), Italy

Stable isotope analysis of carbon (δ¹³C), nitrogen (δ¹⁵N) and sulphur (δ³⁴S) were carried out on one of the largest assemblages of Late Upper Palaeolithic human remains in Southern Europe, at Grotta del Romito (Cosenza), Italy. The burials were stratigraphically dated from ca. 18,000 to 13,000 cal BP, which was confirmed by a series of new AMS dates made directly on the bone collagen. Dietary reconstruction from carbon and nitrogen stable isotopes revealed that eight of the nine individuals analysed, dating to the Final Epigravettian, had very consistent diets, rich in terrestrial animal protein, regardless of their age or sex. These included two individuals who were suffering from severe pathologies. A single individual, dating to the Evolved Epigravettian had a more variable diet, which was significantly enriched in protein from marine or freshwater fish compared to the later burials. Overall, the results are consistent with the very limited number of other studies which describe a change to more specialised and less variable subsistence strategies, in this case the hunting of large herbivores, towards the end of the Palaeolithic period. Sulphur isotope values of all of the nine burials and several faunal samples were notably consistent, showing no evidence of long-distance migration to the site from a different geological zone.     

A palaeodietary investigation of carbon (C/C) and nitrogen (N/N) in human and faunal bones from the Copper Age cemeteries of Varna I and Durankulak,Bulgaria

Stable isotope analyses have been applied to human and faunal bone collagen from the Varna I and Durankulak cemeteries to explore palaeodietary adaptations in the Neolithic and Eneolithic (Copper Age). The results suggest both populations primarily utilised terrestrial, C₃-based diets, despite their proximity to the Black Sea. The wider δ¹⁵N range of the Durankulak humans likely indicates the differential utilisation of terrestrial meat sources, which is probably related to the degree to which primary and/or secondary ovicaprid products were consumed, particularly since ovicaprid δ¹⁵N values differ from other herbivores. The isotopic distribution of Varna I reflects a linear relationship between δ¹⁵N and δ¹³C, suggesting that a minority of individuals enriched in both isotopic parameters supplemented their diets with marine resources. These burials include the well known ‘chieftain’ (burial 43) and show notable material wealth by way of grave goods. At the population level, however, there is no significant correlation between stable isotope values and material wealth at Varna I, a fact with implications for theories regarding emergent social/economic hierarchies in Balkan prehistory. Five burials at Durankulak were found to have relatively enriched δ¹³C and δ¹⁵N values with respect to the rest of the population. These burials reflect a prominently marine-based or mixed terrestrial C₃-based diet that included C₄ inputs, possibly from millet, for which the limitations of stable isotope analysis on bulk collagen are not able to differentiate. AMS dating has shown that these burials belong to a much later period.