Analysis of suspected human coprolites from Terra Amata, Nice, France

Dietary analyses were conducted on 400,000 yr old specimens recovered during the excavation of the Terra Amata Palaeolithic site in Nice, France and initially identified as coprolites. The results of our analyses demonstrate that the identification of these specimens as coprolites is difficult to confirm due to the age of the samples. Consequently, they provide no new data pertaining to the suspected dietary habits of prehistoric man.     

Pollen analysis of coprolites from a late Pleistocene–Holocene cave deposit (Wezmeh Cave,west Iran): insights into the late Pleistocene and late Holocene vegetation and flora of the central Zagros Mountains

Coprolite pollen analysis is increasingly used by palynologists to reconstruct the palaeovegetation especially in the arid to semi-arid regions where lakes and peat bogs are scarce. This study is the first palynological analysis of coprolites in Iran to examine their potential in palaeoenvironmental reconstructions. Four pollen-rich coprolite samples were studied from the Wezmeh Cave in western Iran with a recently discovered late Quaternary (70 ka to sub-recent) faunal assemblage dominated by carnivore remains. Pollen analysis of coprolites shows that a mountain steppe dominated the glacial landscapes of the area. Only one sub-recent sample indicates the presence of dispersed tree stands. One sample was rich in Tulipa pollen suggesting that tulips were relatively abundant during the glacial periods. Cousinia (Asteraceae) pollen was found in all samples with considerable values in one sample indicating its importance in the glacial landscapes of the Zagros Mountains. This study revealed that coprolite pollen analysis can provide an invaluable source of information to understand the floristic composition of palaeolandscapes of the Irano-Turanian region.     

Pervasiveness of phytoliths in prehistoric southwestern diet and implications for regional and temporal trends for dental microwear

Our previous analysis of phytolith content of coprolites showed that calcium oxalate phytoliths from desert food plants caused dental microwear among prehistoric Texas hunter-gatherers. We demonstrated that phytoliths from desert succulents were ubiquitous and abundant in hunter-gatherer coprolites. We found that calcium oxalate phytoliths were harder than human dental enamel. We concluded that phytoliths from desert succulent plants caused dental microwear and hypothesized that such dental microwear would be common in other desert hunter-gatherer and horticultural peoples. Presented here are further analyses of phytoliths from coprolites. Two additional hunter-gatherer sites and three Ancestral Pueblo (Anasazi) horticultural sites are included in this study. Calcium oxalate phytoliths are ubiquitous in coprolites from hunter-gatherer sites in the Mojave Desert and the Colorado Plateau. For the three Ancestral Pueblo sites, calcium oxalate phytoliths from desert succulents (agave family and cactus family) are the most common types of phytoliths encountered. However, silica phytoliths are also present in Ancestral Pueblo coprolites. The data demonstrate that phytoliths from non-cultivated desert plants were a source of dental microwear for the pre-maize Archaic hunter-gatherer bands and maize-reliant Ancestral Pueblo villages.     

Biomolecular and micromorphological analysis of suspected faecal deposits at Neolithic Çatalhöyük,Turkey

Suspected coprolites from midden, burial and room fill contexts at Çatalhöyük were analysed by GC/MS and thin section micromorphology. Assessment of sterol biomarkers enabled a distinction between faecal and non-faecal sources for the deposits to be made, with bile acid biomarkers indicating that many of the faecal deposits are human coprolites. The relative lack of ruminant faeces could be due to this material being used as a fuel source. Deposits in burials were observed to contain soil and plant derived sterols rather than their faecal counterparts. Further analysis in thin section enabled identification of associated materials and contents. Diagnostic inclusions such as bone and plant fragments were only present in some of the human coprolites, which were observed to have a very similar morphology to decayed plant remains. This study illustrates the difficulties in distinguishing coprolites in the field and under the microscope, and demonstrates the importance of integrating biogeochemical methods, particularly when such deposits are used as the basis for interpreting human health and diet, and use-of-space in settlements.     

Identification of fossil hairs in Parahyaena brunnea coprolites from Middle Pleistocene deposits at Gladysvale cave,South Africa

This research focuses on scale pattern and cross sectional morphology of hair to identify an expanded sample of fossil hairs from Parahyaena brunnea coprolites from Gladysvale cave in the Sterkfontein Valley, South Africa. The coprolites are part of a brown hyaena latrine preserved in calcified cave sediment dated to the Middle Pleistocene (257–195 ka). Forty-eight fossil hairs were extracted from 12 coprolites using fine tweezers and a binocular microscope, and examined using a scanning electron microscope. Hair identification was based on consultation of standard guides to hair identification and comparison with our own collection of samples of guard hairs from 15 previously undocumented taxa of indigenous southern African mammals. Samples were taken from the back of pelts curated at the Johannesburg Zoo and Ditsong National Museum of Natural History (formerly Transvaal Museum, Pretoria). Based on the fossil hairs identified here, this research has established that brown hyaenas shared the Sterkfontein Valley with hominins, warthog, impala, zebra and kudu. Apart from humans, these animals are associated with savanna grasslands, much like the Highveld environment of today. These findings support the previous tentative identification of fossil human hair in the coprolites, provide a new source of information on the local Middle Pleistocene fossil mammal community, and insight into the environment in which archaic and emerging modern humans in the interior of the African subcontinent lived.     

Carnivores as zoonotic parasite reservoirs in ancient times: the case of the Epullán Chica archaeological cave (Late Holocene,northwestern Patagonia,Argentina)

Zoonoses are currently considered as one of the most important threats for Public Health worldwide. Numerous zoonoses known today have occurred since antiquity. Carnivores act as definitive hosts for many intestinal parasites; some of them are responsible for several zoonotic diseases. The aim of this work was to study the parasite remains found in coprolites assigned to carnivores from the archaeological site Epullán Chica (ECh) and to discuss the results from a zoonotic point of view. ECh is located in northwestern Patagonia, Argentina and was occupied since the end of the Late Holocene (～2200 years B.P.). Nine coprolites were examined for parasites. Samples were processed by rehydration in a 0.5 % water solution of trissodium phosphate, followed by homogenization, filtered and processed by spontaneous sedimentation. The macroscopic remains were separated and dried at room temperature and were examined for diet analysis. Six out of 9 coprolites examined were positive for parasites. Representatives of at least 10 parasite taxa were registered. Results are in line with the reconstruction of the scenario of zoonoses in the past and the diseases that the human populations and animals from Patagonia could be exposed. The present study provides the first palaeoparasitological report of carnivore coprolites recovered from the archaeological site Ech and reflects contamination of the cave used by hunter-gatherers with different parasites causative of zoonotic diseases.     

Evidence for Seasonality from Coprolites and Recent Faeces?

Abstract

While in search of proof for seasonality from the pollen analysis of coprolites, a comparative study of fox faeces was initiated. Methodology and detailed results of both the coprolites and fox faeces will be published elsewhere, but some critical remarks are presented here. We realise that factors such as sniffing mixtures of pollen from the soil could obscure the results, so that seasonal indicators are difficult to confirm. Exceptions include the special cases in which butterflies and berries had been eaten. They indicate the flowering period of the insect pollinated plants and the fruiting period respectively. Two additional results of the studies were that a tentative reconstruction of the vegetation turned out to be possible, and the presence of cultivated plants could be demonstrated.     

Hunter‐gatherer use of small animal food resources: coprolite evidence

Faunal remains are commonly found in coprolites and provide direct evidence of animal consumption. An evaluation of hunter‐gatherer coprolites from the Southwest US shows that animal bone in coprolites can be used to assess patterns of hunting, food preparation, and general importance of small animals in diet. This is demonstrated by a comparison of faunal assemblages between two hunter‐gatherer sites with respect to small animal hunting strategies. The sites are Dust Devil Cave on the Colorado Plateau, an Archaic winter habitation, and Hinds Cave, a warm season Archaic habitation in the lower Pecos of Texas. The results indicate that small animal hunting varied regionally and seasonally. Copyright © 2006 John Wiley & Sons, Ltd.     

Camelid Gastrointestinal Parasites from the Archaeological Site of Huanchaquito (Peru): First Results

ABSTRACT

Palaeoparasitological investigation was conducted on a first set of samples from 13 sacrificed domestic camelids recovered from the pre-Hispanic Chimú culture site of Huanchaquito-Las Llamas, Peru. The aim was to establish the animals’ gastrointestinal parasite diversity and enlighten on their health status at the time of their death. To this end, 20 samples of coprolites and intestinal contents were analysed to check for the presence of parasite markers, i.e. preserved eggs and oocysts. Microscopic examinations revealed the presence of five taxa of helminths and protozoans in a majority of the tested animals (61%). Our analysis revealed the presence in some animals of protozoan oocysts belonging to the species Eimeria macusaniensis (phylum Apicomplexa). Our study is the first report of the possible presence of a parasite egg attributed to the order Plagiorchiida (family Fasciolidae) in ancient camelids. This preliminary study shows that there is interesting potential for conducting palaeoparasitological analysis at the site and that such analysis is promising for answering questions about the health status of the Huanchaquito-Las Llamas camelids.     

Cluster analysis of the coprolites from antelope house: Implications for anasazi diet and cuisine

This paper reports on a cluster analysis of 155 coprolites from Antelope House, a prehistoric Anasazi site in Canyon de Chelly, Arizona. The analysis revealed three primary clusters; whole kernel maize, milled maize, and nonmaize, which we believe to represent seasonal- and preference-related cuisine. Protein residue analysis on a subsample of the specimens added depth to the analysis.     

Amplification and sequencing of Trichuris trichiura ancient DNA extracted from archaeological sediments

Despite high prevalence of Trichuris trichiura infection, PCR-based analysis on T. trichiura from archaeological samples has not been established so far. In the present study, we sought to perform PCR-based amplification of T. trichiura aDNA using the sediments from medieval tomb of Korea. The presence of Trichuris eggs were first detected by microscopic observation; then confirmed by PCR-based aDNA analysis. Obtained sequence showed 100% homology to that of the small subunit ribosomal RNA (SSUrRNA) gene of T. trichiura but distinct from that of other Trichuris species. PCR-based aDNA analysis in this study can serve as effective method to confirm the presence of T. trichiura eggs in the soils or coprolites collected from archaeological sites.     

Late Triassic palynoflora from the Pranhita–Godavari Valley,India: evidence from vertebrate coprolites

Vijaya, Prasad, G.V.R. & Singh, K., June, 2009. Late Triassic palynoflora from the Pranhita–Godavari Valley, India: evidence from vertebrate coprolites. Alcheringa 33, 91–111. ISSN 0311-5518.

The Upper Triassic Maleri Formation, represented by red clays and sandstones, has to date not produced any plant macrofossils or palynomorphs. Many spiral and non-spiral coprolites collected during this study from the Maleri Formation of the Pranhita-Godavari Valley were analysed for palynomorphs. Based on shape, nature of coiling and size, the Maleri coprolites are classified into seven groups. Of these, only Group-I, Type 5 (non-spiral) and amphipolar (spiral) types yielded diverse gymnospermous and pteridophytic spores, pollen, other plant debris and sparse fungal spores and algal remains. Occurrences of Antulsporites varigranulatus, Aratrisporites spp., Cadargasporites baculatus, Dubrajisporites isolatus, Enzonalasporites vigens, Foraminisporis coelatus, Grandispora spinosa, Kraeuselisporites saeptatus, Polycingulatisporites reduncus, Staurosaccites spp., Tethysispora unica and Tikisporites balmei confirm a Late Triassic age for the coprolite-bearing red clays. Records of Classopollis classoides and Callialasporites turbatus/dampieri in these assemblages more precisely suggest a Norian to Rhaetian age. The non-spiral coprolites were possibly produced by aquatic piscivorous animals whereas the spiral coprolites may have been produced by an as yet unidentified fish taxon. The coprolite-producing animals (spiral and non-spiral groups) possibly ingested gymnospermous and pteridophytic plant remains passively along with water or their herbivorous prey.     

Evaluating chloroplast DNA in prehistoric Texas coprolites: medicinal,dietary, or ambient ancient DNA?

Molecular analysis of coprolites from Hinds Cave, Texas recovered chloroplast DNA sequences. The sequences were interpreted as evidence of diet. We analyzed 19 Hinds Cave coprolites to evaluate the potential sources of the chloroplast DNA (cpDNA) and compared our results to previous studies. This review shows that some cpDNA sequences could be from well-known prehistoric plants foods. Some other sequences could have come from ambient plant material in the guts of small animals eaten by humans in antiquity. Using pollen concentration analysis, we identify sources of ambient plant material which could have been inhaled or imbibed. It is even possible that cpDNA sequences are from proplastids within ambient pollen grains themselves. However, three sequence types cannot be explained as resulting from only dietary or ambient sources. We suggest instead that these might be from medicinal or hallucinogenic plants. We compared these three sequences to existing sequences in the GenBank. We found that these sequences are 100% matches for Rhamnus, Fouquieria, and Solanum.     

High Resolution Stratigraphic Distribution of Coprolites within Eneolithic Middens,a Case Study: Hârsova-Tell (Constanta County,Southeast Romania)

Abstract

Quantitative distribution of coprolites, fish bones and mammal bones recovered by wet screening from an Eneolithic midden at Hârsova-tell (southeast Romania) is surveyed within a high resolution stratigraphic framework. The coprolites, all containing bone fragments, are attributed to carnivores/omnivores: dog, pig, man. Highly significant correlation (Spearman's coefficient of rank correlation r_s = 0.46, P < 0.0001) between coprolite distribution and the seasonality-related fish bone distribution suggests that seasonality controls the stratigraphic distribution of coprolites recovered by wet screening within middens. Periods of high fish bone input into the midden, correlated with frequent coprolite occurrences, correspond to the warm season (late spring to early autumn). Assessment of potential factors controlling coprolite distribution within the midden suggests that sections of high coprolite concentration reflect preservation of faeces until embedding in sediments, and/or rapid embedding of faeces. Preservation of faeces is favored by dry periods, characteristic of the summer season in the highly continental climate of the region. Rain, freeze-thaw cycles or other wet season processes favor faeces disintegration. Our results suggest that the stratigraphic distribution of coprolites obtained by wet screening may represent an indicator of seasonality in middens. Additional studies are needed to better characterise this relationship and refine interpretations.     

Romano-Libyan Dryland Animal Husbandry and Landscape: Pollen and Palynofacies Analyses of Coprolites from a Farm in the Wadi el-Amud,Tripolitania

Pollen and palynofacies from sheep/goat, rodent and human coprolites of Romano-Libyan, Arab and recent age from a farmstead in the Wadi el-Amud, Tripolitania, Libya are described. There are few differences between assemblages from modern sheep/goat dung and from recent aeolian sand—both contain high percentages of desert species and significant far-travelled pollen. The Romano-Libyan and Arab samples contain high percentages of steppe taxa, suggesting significant vegetation change has occurred subsequently. Romano-Libyan sheep/goat dung samples are often dominated by single taxa, suggesting that the grazing of these animals was closely controlled or that they were stalled and fed hay, cereal waste or other crop residue. This practice seems to have contributed to the longevity of a mixed-Mediterranean agroecosystem in the predesert by preventing widespread and indiscriminate grazing and consequent landscape destabilization.     

An exclusively hyena-collected bone assemblage in the Late Pleistocene of Sicily: taphonomy and stratigraphic context of the large mammal remains from San Teodoro Cave (North-Eastern Sicily,Italy)

A detailed taphonomic analysis of the large mammal assemblage from 1998 to 2006 excavations at San Teodoro Cave is presented, taking into account the stratigraphic context of the deposits. Three not strictly contemporary fossiliferous levels having different lithological features have been detected, here named B-I, B-II, and B-III. Fossil remains are prevalently accumulated in B-I and B-II. The three levels are characterized by evidence of Crocuta crocuta spelaea occupation, represented by their skeletal remains, coprolites, and distinctive damages on the bones, similar to fossil and modern spotted hyena dens from Europe and Africa. A differential distribution of coprolites and small digested bones, probably due to different humidity conditions, has been recognized in B-I and B-II, and can be related to different topographic locations within the cave or to different climate conditions during the sedimentation phases. The very low density of fossil remains in B-III, which is the oldest level, could indicate an area that was less inhabited by hyenas, probably due to geomorphological conditions. Taphonomic comparison of the three fossiliferous levels of the San Teodoro Cave deposits points to a long-term, perhaps cyclic, occupation of the cave by hyenas and confirms the cave as one of the most important Pleistocene hyena dens in Europe.     

Repeat silica extraction: a simple technique for the removal of PCR inhibitors from DNA extracts

Polymerase chain reaction (PCR) inhibitors are often co-extracted with ancient DNA (aDNA) and when present make the analysis of aDNA difficult, if not impossible. In this study we review previous research on PCR inhibitors and techniques that address their co-extraction with DNA from sub-optimal samples. Additionally, we introduce a simple extraction technique, “repeat silica extraction,” that effectively removed PCR inhibitors from extracts of 7000–8000-year-old human skeletal remains from the Windover archaeological site in Florida and 700–2000-year-old human coprolites excavated from Fish Slough Cave in southern California. A series of tests on these same samples demonstrates that N-phenacylthiazolium bromide is largely ineffective, despite previously reported success using this compound as part of the DNA extraction process. We also describe a method for demonstrating the presence as well as successful removal of PCR inhibitors by use of a “positive aDNA control,” a test necessary to conclude that negative PCR amplification is the result of the absence of preserved DNA.     

Combining photogrammetry and laser scanning for the recording and modelling of the Late Intermediate Period site of Pinchango Alto,Palpa, Peru

This paper describes the 3D modelling of Pinchango Alto, Peru, based on a combination of image and range data. Digital photogrammetry and laser scanning allow archaeological sites to be recorded efficiently and in detail even under unfavourable conditions. In 2004 we documented Pinchango Alto, a typical site of the hitherto poorly studied Late Intermediate Period on the south coast of Peru, with the aim of conducting spatial archaeological analyses at different scales. The combined use of a mini helicopter and a terrestrial laser scanner, both equipped with a camera, allowed a fast yet accurate recording of the site and its stone architecture. In this paper we describe the research background, the 3D modelling based on different image and range data sets, and the resulting products that will serve as a basis for archaeological analysis.     

Huerequeque: An inland outpost of the Initial Period Sechín Alto Polity in the Casma Valley of Peru

The Sechín Alto Polity effectively controlled the Casma Valley zone on the Peruvian north coast during the Initial Period (2100–1000 cal b.c.) through an inclusive administrative strategy whereby large inland centers and their component monumental architecture served specific, complementary functions within the polity. Smaller satellite sites established on the coast insured a supply of marine protein to the inland agricultural centers. The political strategy of the Sechín Alto Polity also included expansion inland to colonize the mid-sized site of Huerequeque at a key location in order to annex abundant agricultural land, to access a trade route to the highlands, and to extend and define the eastern Sechín Alto Polity boundary farther. Huerequeque is unique, however, because of its distinct cultural heritage, and excavations there have made it possible to examine the process through which the expanding polity established an administrative presence, imposed Sechín Alto Polity practices, and eventually abandoned the site. More broadly, elucidating this process is critical to a better understanding of the development and expansion of complex societies.     

Identifying foreigners versus locals in a burial population from Nasca, Peru: an investigation using strontium isotope analysis

During the several thousand years of human occupation in the Nasca drainage on the south coast of Peru, population movement into and out of the region impacted the formation and organization of society, as well as contributed to major cultural transformations. This study identifies foreign versus local individuals through the investigation of burial practices and strontium isotope analysis of human remains from the sites of La Tiza and Pajonal Alto dating from the Early Intermediate Period through the Late Intermediate Period (A.D. 1–1476). Of the ten samples analyzed, two individuals dating to the Middle Horizon were identified as foreigners. One of these individuals was buried in a new elite tomb type providing additional evidence that the Wari state incorporated Nasca into its realm. Two headless individuals, one dating to Middle Nasca with clear evidence of decapitation, and another dating to the Late Intermediate Period who was likely decapitated, were both locals. This suggests the practice of decapitation took place among local groups, and that the practice of decapitation and the use of heads as ritual items or trophies may have continued later in time than previously thought.