Stable isotope palaeodietary study of humans and fauna from the multi-period (Iron Age,Viking and Late Medieval) site of Newark Bay,Orkney

We report here on stable carbon and nitrogen isotope measurements of human and faunal bone collagen from the Iron Age, Viking Age, and Late Medieval site of Newark Bay, Orkney, Scotland. We found a wide range of results for humans in both δ¹³C (−15.4‰ to −20.3‰) and δ¹⁵N (8.6‰–15.6‰) values. The enriched carbon and nitrogen values indicate the consumption of significant amounts of marine protein, which is very unusual for post-Mesolithic (e.g. 4000 cal BC) UK and European populations. Also of interest is a statistically significant difference in δ¹³C (t = −2.48, p = 0.011) and δ¹⁵N (t = −2.44, p = 0.011) values, and therefore diets, between adult males and females at this site, with males (δ¹³C = −17.8 ± 1.2‰, δ¹⁵N = 13.2 ± 1.6‰) having, on average, a higher proportion of marine protein than females (δ¹³C = −18.9 ± 1.1‰, δ¹⁵N = 11.8 ± 1.8‰). The weaning age of the sub-adults was difficult to interpret due to the large isotopic variation in the adult females, but nearly all individuals between birth and 1.25 years have elevated δ¹⁵N values indicating that they were breastfed to some extent.     

Geographic variation in bone carbonate and water δO values in Mendoza,Argentina and their relationship to prehistoric economy and settlement

Questions of mobility, settlement pattern, and their relation to economic organization and resource use are central to ongoing work in central-western Argentina. Here we analyze geographic patterns in the distribution of 178 human bone carbonate δ¹⁸O samples, 46 human tooth enamel carbonate δ¹⁸O samples, and 48 water δ¹⁸O samples from throughout the Andean Cordillera and Monte Desert and evaluate their implications for prehistoric mobility and economy. We confirm and refine previous generalizations regarding a highland/lowland dichotomy in water δ¹⁸O values and show that the range of human carbonate δ¹⁸O values generally reflect available water sources. While there is little within-lifetime change in patterns of water use, we show that most individuals have stable isotope signatures consistent with water use from multiple areas or areas other than where they were ultimately interred. These data indicate high levels of residential mobility, and we conclude by discussing their implications for our understanding of regional prehistory.     

Late Mesolithic burials at Casa Corona (Villena,Spain): direct radiocarbon and palaeodietary evidence of the last forager populations in Eastern Iberia

Current knowledge about the Mesolithic–Neolithic transition in the Central and Western Mediterranean European regions is deeply limited by the paucity of Late Mesolithic human osteological data and the presence of chronological gaps covering several centuries between the last foragers and the first archaeological evidence of farming peoples. In this work, we present new data to fill these gaps. We provide direct AMS radiocarbon dating and carbon (δ¹³C) and nitrogen (δ¹⁵N) stable isotope analysis were carried out on bone collagen samples of two single burials from the recently discovered open-air Late Mesolithic site of Casa Corona (Villena, Spain). The results shed new light on the chronology and subsistence patterns of the last Mesolithic communities in the Central Mediterranean region of the Iberian Peninsula. Radiocarbon results date the human remains and funerary activity of the site to 6059–5849 cal BC, statistically different from other Late Mesolithic sites and the earliest Neolithic contexts, and bridging the 500 yrs chronological gap of the Mesolithic–Neolithic transition from the area. Isotopic evidence shows that diet was based on terrestrial resources despite the proximity to the site of lagoon and marine ecosystems. This and previous isotope studies from the region suggest a lower reliance upon marine resources than for Atlantic and Cantabrian sites, although intra-regional patterns of neighbouring Mesolithic populations exhibit both fully terrestrial diets and diets with significant amounts of aquatic resources in them. We hypothesize that in the Central Mediterranean region of Spain the Late Mesolithic dietary adaptations imposed structural limits on demographic growth of the last foragers and favoured rapid assimilation by the earliest Neolithic populations.     

Paleodiet reconstruction of Bronze Age Siberians from the mortuary site of Khuzhir-Nuge XIV,Lake Baikal

Paleodiet reconstruction using stable isotopes of carbon and nitrogen was carried out on individuals from the Bronze Age cemetery, Khuzhir-Nuge XIV on the western shore of Lake Baikal, Russian Federation. The cemetery contained 79 graves with 89 individuals and was in use between approximately 2700 and 2000 cal. B.C. with the exception of one burial, dated to around 4000 B.C. Archaeological and ethnographic evidence indicates that hunting and fishing were the main subsistence activities while gathering was less important. δ¹³C from collagen and carbonate apatite was analysed, along with δ¹⁵N from collagen. Bone mineral preservation was evaluated using Fourier transform infrared spectroscopy. δ¹³C and δ¹⁵N of collagen are positively correlated and reflect varying reliance on terrestrial mammals, fish, and seals from Lake Baikal. Lake Baikal biota exhibit considerable variation in δ¹³C at the base of the food web thus; there is variation in human δ¹³C (−20.1‰ to −16.2‰) despite the fact that there are no C₄ plants in the region. δ¹⁵N results (range 10.3‰–16.5‰) indicate that fish, and possibly seals, made up some of the diet for everyone, but in varying amounts relative to terrestrial herbivores. δ¹³C from carbonate is not correlated with δ¹³C from collagen, a situation that has been found in remains from other coastal sites. A mixed diet from both terrestrial and freshwater ecosystems is the most likely explanation. Analysis of mortuary variables within the cemetery reveals three distinct clusters of graves with variations in burial treatment among the clusters. δ¹⁵N values are significantly different among these clusters, suggesting an association between diet and mortuary customs.     

Stable isotope analysis of dog,fox, and human diets at a Late Holocene Chumash village (CA-SRI-2) on Santa Rosa Island,California

Stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope analyses of dog (Canis familiaris), island fox (Urocyon littoralis), and human bone collagen from CA-SRI-2 (AD 130–1830) on Santa Rosa Island, California provide a proxy of diet and the relationships between humans and these animals. Carbon isotopic signatures indicate that Native Americans and their dogs at CA-SRI-2 subsisted almost exclusively on marine resources, while the island fox ate primarily terrestrial foods. Nitrogen isotopes and archaeofaunal remains indicate that humans and dogs also ate higher trophic level foods, including finfishes, marine mammals, and seabirds with smaller amounts of shellfish. The CA-SRI-2 island foxes appear to have eaten higher amounts of terrestrial foods, similar to the diets observed in modern fox populations. These data generally confirm the commensal relationship assumed to exist between domesticated dogs and people, but the carbon isotopic composition of dogs is enriched ∼2‰ compared to humans. We hypothesize that the difference in carbon isotopes between dogs and humans may have resulted from a higher consumption of C3 plants with lower δ¹³C values by humans, or less likely from the ingestion by dogs of significant amounts of bone collagen, which is enriched by ∼4‰ over associated muscle.     

Mesolithic shellfish exploitation in SW Italy: seasonal evidence from the oxygen isotopic composition of Osilinus turbinatus shells

Oxygen isotope analyses were carried out on the topshell Osilinus turbinatus from archaeological sites with the aim to investigate the temporal exploitation patterns by Mesolithic groups inhabiting coastal caves in SW Italy. In order to assess the present day O. turbinatus intra-annual δ¹⁸O variability, living specimens were collected monthly at Marina di Camerota (SW Italian peninsula). Their shell-edges were analysed and the seasonal results were compared with archaeozoological material. Our findings show that Mesolithic O. turbinatus exploitation was carried out almost exclusively during the colder and intermediary seasons (e.g. autumn, winter, spring), with very sporadic harvesting during the warmer seasons. Results suggest a possible regional exploitation pattern of O. turbinatus by Mesolithic groups inhabiting this region.     

Patterns of Resource Use and Isotopic Niche Overlap Among Guanaco (Lama guanicoe), Pampas Deer (Ozotoceros bezoarticus) and Marsh Deer (Blastocerus dichotomus) in the Pampas. Ecological,Paleoenvironmental and Archaeological Implications

ABSTRACT

In this paper, we analyze the isotopic niche and resource use of the guanaco (Lama guanicoe), pampas deer (Ozotoceros bezoarticus) and marsh deer (Blastocerus dichotomus) on the temperate plain of the northern Pampa region in the southeast of South America. The measured stable isotope compositions in bone tissue are δ¹³C, δ¹⁵N and δ¹⁸O. All the analyzed samples were recovered from different archaeological sites from the late Holocene. According to the results, the guanaco was strictly confined to the Pampa plain environment, developing a broad niche based on C₃ plants and a variable contribution of C₄ grasses within a grazing trend. The pampas deer preferentially used the Pampa plain, but also the prairies on the borders of the wetland, showing eurioic characteristics and a narrower niche based on C₃ plants. In turn, the marsh deer was strictly confined to wetland environments, developing a C₃ diet–base, within a narrow isotopic niche and stenoic characteristics. The three mammals showed a wide range of intraspecific variability, which was a key factor in their adaptability to spatial and temporal changes in the vegetation coverage. In fact, the temporal trends of their isotopic values were concurrent with the major climatic variations of the Holocene. Differences in the correlations between the values of both carbon sources in both deer species compared with the guanaco suggest a distinct chemical composition of their diet and/or differences in the allocation of nutrients. The isotopic values of nitrogen and spacing of the carbon sources in guanaco (pseudoruminant) and both deer species (ruminants) show no significant differences between them, thus establishing the values for local large herbivores. Significant correlations between δ¹³C and δ¹⁵N were found in the guanaco and marsh deer. The regional and extra–regional variability in the guanaco's δ¹³C_collagen and δ¹⁵N probably reflect the clinal variations in the vegetation coverage and the amount of rainfall. The collagen isotope values in the guanaco throughout the entire Holocene show that the humid Pampa would have shifted between being a more recurrent mesic and temperate plain with minor phases of dry–mesic conditions like during the Little Ice Age, and a humid and warm one at the peaks of the Holocene Thermal Maximum and the Medieval Climatic Anomaly. The guanaco and the pampas deer adapted to all the climatic changes that happened on the humid Pampa until the biological invasions of large European mammals changed the herbivores’ guild composition of this vast plain, pushing them into peripheral habitats due to competition. The within–species variability in isotopic signals through time and space make it necessary to carry out adequate sampling before reconstructing the diets of local past populations.     

Reassessing the northern limit of maize consumption in North America: stable isotope,plant microfossil,and trace element content of carbonized food residue

In combination, the analysis of carbonized food residue for stable C and N isotopes, elemental composition, and plant microfossils (phytoliths and starch granules) offers a powerful tool for understanding patterns of prehistoric maize (Zea mays) consumption in small-scale societies. Using this approach, we conclude that maize was more widely consumed in North America than previously suspected. Between ∼AD 700 and 1600, despite little or no archaeological evidence of gardening, corn was a widespread component of diet on the eastern Canadian prairies. This pattern, furthermore, extended into the adjacent boreal forest at about the same time. However, carbon (δ¹³C) and nitrogen (δ¹⁵N) values on food residue samples vary widely, suggesting significant overall dietary differences from one region to the next. Analysis of a subset of residue samples for elemental composition (using ICP-OES [inductively coupled plasma–optical emission spectrometry]) may help identify broad trends in the provenience of foods consumed at these sites.     

Seasonality of human site occupation based on stable oxygen isotope ratios of cod otoliths

Oxygen isotope ratios (δ¹⁸O) were measured in micromilled samples of cod otoliths recovered from two Stone Age sites, one from Skoklefald, southeastern Norway and five from Skipshelleren, southwestern Norway. Discrete samples of otolith material were milled from the edge of the otolith towards the centre, each sample representing 3–4 weeks of growth. The δ¹⁸O values showed seasonal signals, covering a period 1–2 years prior to the time of capture. Isotope ratios at the edge of the otoliths, which is the material deposited at the time of fish capture, corresponded to the coldest seasonal water temperatures, in late winter or early spring. These data provide independent evidence for the season of use of the Skoklefald and Skipshelleren sites.     

Diversity in foddering strategy and herd management in late Bronze Age Britain: An isotopic investigation of pigs and other fauna from two midden sites

Abstract

Middens of the southern British late Bronze and Iron Age are vast accumulations of cultural debris that can be explained as refuse dumps linked with large periodic feasting events. A distinctive feature of these sites is that their faunal assemblages invariably comprise a considerably higher proportion of pig remains than contemporaneous settlement sites. This paper presents results from a programme of stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope analysis of fauna from two major midden sites, Llanmaes in South Wales and Potterne in Wiltshire. The research aim is to reconstruct husbandry strategies and foddering regimes, particularly concerning pigs, to better understand how the challenges of raising large herds were met. Analysis produced exceptionally wide-ranging results for pigs and other domesticates at both sites, particularly in terms of δ¹⁵N values, demonstrating that diverse foddering strategies were employed. Diversity in the late Bronze Age pig foddering regimes indicates that the Neolithic husbandry practices (focusing on woodland fodder) had not been abandoned, but that new husbandry methods (consumption of household waste) were also being practised, which subsequently became more widely established in the Iron Age. The heterogeneity of signatures suggests that animals may have been husbanded in a piecemeal fashion at a local, household level. This in turn hints that fauna may have been brought to these sites from households across the surrounding landscape, rather than being husbanded by specialist producers in the vicinity of the middens.     

Stable isotopes and diet at Ancient Kerma,Upper Nubia (Sudan)

Carbon and nitrogen stable isotope values were measured from bone collagen extracted from archaeological Nubian human (n = 54) and faunal (n = 61) populations from the site of Kerma, Sudan. Collagen suitable for isotopic analysis was extracted from 22 faunal and 48 human samples from the Eastern cemetery site, dated to the Middle Kerma (c. 2050–1750 BC) and Classic Kerma (c. 1750–1500 BC) periods respectively. The isotopic data indicate that the human dietary regimen included a mix of C₃ and C₄ plant-derived components, with a larger C₄ component than previously reported in archaeological Egyptian Nile Valley populations. Elevated δ¹⁵N values are attributed to consumption of dietary resources from a ¹⁵N-enriched terrestrial ecosystem. The faunal isotope data also indicate the consumption of C₃ and C₄ plants. The large range of δ¹³C values measured in both the human and faunal samples supports previous work suggesting that a significant portion of the populations buried at Kerma may have originated elsewhere, further confirming the Nile Valley as a major corridor for population movement in the region.     

Investigating the dietary life histories and mobility of children buried in St Gertrude Church cemetery,Riga, Latvia, 15th–17th centuries ad*

Carbon and nitrogen isotope profiles were obtained from incremental dentine analysis of 19 non-adults from a cemetery in Riga, Latvia. The research compared the life histories and diet between people buried in two mass graves and the general cemetery. The δ¹³C profiles of several children from the mass graves were similar but did not resemble the patterns seen in children from the general cemetery, suggesting that they probably represented a different population group. The rise in δ¹⁵N values towards the end of the life of four individuals from one mass grave suggests they were victims of an historically documented famine.     

Reconstruction of Socioeconomic Status in the Medieval (14th–15th Century) Population of Grevenmacher (Luxembourg) Based on Growth,Development and Diet

Numerous historical sources describe many aspects of the estate‐based society in medieval times; the detection of socioeconomic status within populations through skeletal remains is a topic of growing interest in anthropological studies. In medieval times, it was common for members of high social rank to be buried within or next to a church. This was certainly the case in Grevenmacher (Luxembourg), where remains of a church building and an adjacent cemetery from the 13th until the beginning of the 15th century were subjected to archaeological and anthropological analysis. By integrating archaeological and anthropological elements, as well as stable isotope analysis, we documented osteological manifestations as indicators of diet differentials in two subsamples (first group consists out of 56 individuals, second one out of 184 individuals) from the medieval cemetery of Grevenmacher. We could distinguish two subsamples that we assumed as different in their socioeconomic status with regards to the burials' position in the cemetery and burial characteristics. Differences in osteological traits such as bone length, stature and body mass indicated differences in diet between sample groups. To substantiate these outcomes, stable isotopic analysis (δ¹³C, δ¹⁵N) were made; the results displayed a clear separation between the sample groups on the basis of their diet. Therefore, we were able to confirm certain individuals in the medieval population of Grevenmacher in relationship to their socioeconomic status. Copyright © 2017 John Wiley & Sons, Ltd.     

Isotopic anthropology of rural German medieval diet: intra- and inter-population variability

This study investigates the diet of an eleventh century CE parish community located in northwestern Germany. We assessed the isotopic compositions of human (n = 24) and faunal (n = 17) bone collagen (δ ¹³C_col, δ ¹⁵N_col) and human structural carbonate (δ ¹³C_sc) using skeletal material recovered from the Dalheim cemetery. Traditional interpretation of the isotopic data indicates that Dalheim residents likely relied on a C₃ plant-based diet and consumed some terrestrial animal products without evidence of marine resource input in the diet. Bivariate and multivariate models used as an additional means to assess diet indicate minor consumption of C₄ plant foods in this community. The multivariate-isotope model identified regional similarities and differences in C₄ plant/marine food consumption and in dietary protein sources by comparing data from Dalheim with those of other medieval sites from the published literature. We did not observe sex differences in this population but differences in δ ¹⁵N_col suggest that juveniles consumed the lowest trophic level protein.     

New Insights into Mesolithic Human Diet in the Mediterranean from Stable Isotope Analysis: The Sites of Campu Stefanu and Torre d'Aquila,Corsica

Mesolithic human remains are rare in the archaeological record of the French Mediterranean. Only the island of Corsica has so far produced relatively well‐preserved burials, and recent archaeological excavations have brought to light new Mesolithic human remains. The site of Campu Stefanu , located in Sollacaro in the southeast of the island, contained a collective burial of seven to eight individuals in a previously unobserved funerary context. A re‐evaluation of collections in regional museums yielded the remains from another Mesolithic individual from the site of Torre d 'Aquila , excavated at Pietracorbara, in the northern part of the island, at the beginning of the 1990s. These two discoveries presented the rare opportunity to obtain new radiocarbon dates and paleodietary insights from this crucial time period using stable isotope analysis (δ¹³C, δ¹⁵N) on collagen. From Campu Stefanu, one individual had sufficient collagen preserved for radiocarbon dating, revealing that it is the oldest Mesolithic human known on the island, dated to 10216–9920 cal. BP. At Torre d'Aquila, radiocarbon dates indicate that the individual belonged to a younger Mesolithic phase than Campu Stefanu, dated to 9903–9596 cal. BP. δ¹³C and δ¹⁵N isotope ratios are similar between the Campu Stefanu and Torre d'Aquila individuals and indicate a diet dominated by the consumption of terrestrial animal protein and a lack of marine resources. These findings are in contrast with the previous results from two other Mesolithic individuals from Corsica from the sites of Araguina Sennola and Monte Leone , for which about 25–30% of the consumed proteins came from a marine diet. The dietary variability recorded in Corsica is consistent with results obtained from Mesolithic human remains of Sicily and the Iberian Peninsula. We can hypothesise, that despite the nomadic lifestyle, the distance to the sea played a major role in Mesolithic food choices in Corsica. Copyright © 2016 John Wiley & Sons, Ltd.     

Isotopic evidence for the reconstruction of diet and mobility during village formation in the Early Middle Ages: Las Gobas (Burgos,northern Spain)

Strontium, carbon, and nitrogen isotopes of human bone and tooth remains have been used to reconstruct residential mobility and diet of early medieval populations at Las Gobas from the sixth to eleventh centuries. Most non-local individuals correspond to the tenth to eleventh centuries and were mostly women and infants. This residential mobility coincided with the formation of Laño village and the abandonment of artificial cave settlement. Carbon and nitrogen isotope ratios of bone collagen indicate an omnivorous homogenous diet based on terrestrial plant resources, with few animal-derived proteins from livestock. Millet consumption was restricted to an earlier period of time (seventh to ninth centuries); and in later periods (tenth to eleventh centuries), mainly C₃ plants such as wheat and barley were consumed. In general, there were no dietary differences between individuals according to sex or age. Sex-related dietary differences have only been observed in the tenth to eleventh centuries, when females consumed a more vegetarian diet and less animal protein. The higher δ ¹⁵N values in infants reflect the weaning effect, while the differences in δ ¹⁵N values between young adult men and young adult women can be explained as a physiological factor related to pregnancy or different origins. In a comparison with contemporaneous medieval populations in the northern Iberian Peninsula, both δ ¹³C and δ ¹⁵N values suggest similar foodstuff resources and diet among Christian and Muslim populations.     

Calcium isotopes in archaeological bones and their relationship to dairy consumption

The calcium isotope ratios (δ^44/42Ca) of bones from humans and fauna from three archaeological sites, Taforalt, Abu Hureyra, and Danebury, are evaluated in order to assess whether calcium isotope ratios of bones can be used to detect dairy consumption by adult humans. At each site the fauna δ^44/42Ca is the same regardless of species, while the humans have lower δ^44/42Ca than the local animals by 0.24–0.41‰ (site means). However we cannot ascribe this difference to dairy consumption, given this human–faunal difference also occurs in Epipalaeolithic and Mesolithic adult humans, where dairy consumption is unlikely. Rather, this difference appears to be a result of differences in metabolic processes or other aspects of diet between humans and fauna. Minimal isotopic change in sequential acid leaches of bone powders and consideration of the high calcium concentration in bone suggest that bone calcium isotope ratios are not substantially affected by diagenetic change.     

Diet in medieval Denmark: a regional and temporal comparison

The aim of this research is to explore regional and temporal patterning in diet in medieval Danish populations. δ¹⁵N, δ¹³C_coll, δ¹³C_ap values were obtained from 154 human bone samples from three sites located in the Jutland peninsula of Denmark. These sites span the medieval period. The results suggest that the medieval Danish diet was composed of C₃ plants, terrestrial animals, and freshwater and marine fish in varying amounts. The data also suggest some regional patterning in diet, but little temporal differences in the composition of diet.     

Human subsistence strategy at Liuzhuang site,Henan, China during the proto-Shang culture (∼2000–1600 BC) by stable isotopic analysis

Since the discovery of the proto-Shang culture, created mostly by ancestors of the Shang clan from the late Neolithic Age to the early Shang period (∼2000–1600 BC), the subsistence strategy and lifestyle of humans in China during their movement southwards have been a great focus. Chinese literature and archaeological findings suggest that the proto-Shang societies were composed of different cultural groups and had various subsistence strategies. For example, at the Liuzhuang site, three types of burials, i.e., stone coffin, wooden coffin and earthen shaft-pit, are found. The wooden coffin and earthen shaft-pit burials had been adopted locally in the Central Plains since the Neolithic Age while the stone coffin burials were usually used by people living in Northeast China and had never been found in the Central Plains before. In this study, stable carbon and nitrogen isotopic analyses were performed on human bones from the Liuzhuang site and animal bones from Zhangdeng site in Henan province, China to determine whether different social groups had various accesses to food resources and whether their dietary difference was related to inequality in social status. Humans have mean δ¹³C and δ¹⁵N values of −7.6 ± 0.6‰ and 9.6 ± 1.0‰ (n = 19) respectively, which strongly indicates that humans rely primarily on C₄-based food. The main contribution of C₄-based food in their diet is from millet agriculture or animals that consume millet by-products. The isotopic spacing of carbon and nitrogen isotopic values between pigs and humans, between dogs and humans, and between cattle and humans, all imply that these animals were the main meat resources for humans. Surprisingly, the δ¹³C values and δ¹⁵N values of humans with different types of burials are quite close, indicating that they had equal access to food resources. This result suggests that the proto-Shang humans had adapted to the local subsistence strategy, and the local cultural factors in the Central Plains were very well integrated into proto-Shang culture.     

Stable carbon and nitrogen isotope analysis on human remains from the Early Mesolithic site of La Vergne (Charente-Maritime,France)

We report here the results of stable carbon and nitrogen isotope analysis of human and faunal remains from La Vergne (Charente-Maritime, western France), a rare Early Mesolithic burial site (ca. 8500–8000 cal BC). The results for nine humans (average δ¹³C = −19.3‰; δ¹⁵N = 9.4‰) indicate a strongly terrestrial diet, dominated by animal protein, with the possibility of, at best, a slight contribution of marine-derived protein. Given lower sea-levels in the early Holocene, the site would have been some 60–80 km from the sea at the time of its use; nevertheless, contacts with the coast are shown by the presence of numerous marine shell beads in the graves. In the light of the stable isotope results, it is suggested here that such contacts most likely took the form of exchange with coastal communities whose remains now lie underwater.