Measuring Root Dentine Translucency in Human Teeth of Varying Antiquity

Many workers have calibrated human root dentine transparency (RDT) as a linear regression on age. It is now regarded as a well-established means of estimating age at death in modern human material. Similar applications in archaeological material have not yet been developed. The aim of this study was to establish a standard protocol for measuring RDT which was derived from previous methods and which could be applied to teeth of unknown and varying antiquity. An initial study on two archaeological populations determined the choice of tooth to study and a second study, using expendable teeth (of unknown age and origin), evaluated various techniques of specimen preparation and examination. Findings from the pilot study indicated that the lower canine was the tooth of choice. From the second study it was observed that archaeological teeth could only be sectioned if they had first been infiltrated and embedded in methyl methacrylate. The optimal section thickness was found to be 150 μm and no benefit was gained by staining. Inter-observer reliability tests showed significant differences in repeated measures of RDT in intact teeth, which were not borne out when sectioned teeth were used. Intra-observer reliability was maintained for measurements in both intact and sectioned teeth. These findings have been used to establish a standard protocol for application to human teeth of any depositional phase to estimate the dental age at death of that individual.     

Recording dental caries in archaeological human remains

Dental caries is an important condition to record in archaeological collections, but the way in which recording is carried out has a large effect on the way in which the results can be interpreted. In living populations, dental caries is a disease that shows a strong relationship with age. Both the nature of carious lesions and their frequency change with successive age groups from childhood to elderly adulthood. There is also a progression in the particular teeth in the dentition which are most commonly affected and, in general, the molars and premolars are involved much more frequently than the canines and incisors. Lower teeth are usually affected more than upper, although the condition usually involves the right and left sides fairly equally. In the high tooth wear rate populations represented by many archaeological and museum collections, there is a complex relationship between the form of lesions and the state of wear, which adds yet another range of factors to the changing pattern of caries with increasing age. In the same populations, chipping, fracture and anomalous abrasion of teeth are also common, and these contribute similarly to the distribution and forms of carious lesion observed. Amongst the living, the pattern of ante‐mortem tooth loss is important in understanding caries and, in archaeological material, there is also the complicating factor of post‐mortem tooth loss. Finally, there is the question of diagnosis. There are diagnostic problems even in epidemiological studies of living patients and, for archaeological specimens, diagenetic change and the variable preservation of different parts of the dentition add further complications. For all these reasons, it is difficult to define any one general index of dental caries to represent the complete dentition of each individual, which would be universally suitable for studying a full range of collections from archaeological sites or museums. Variation in the nature of collections, their preservation, tooth wear, and ante‐mortem and post‐mortem tooth loss mean that when such a general index appears to differ between sites, there could be many other reasons for this, in addition to any genuine differences in caries incidence and pattern that might have been present. It is suggested here that the best approach is instead to make comparisons separately for each tooth type, age group, sex, lesion type and potential lesion site on the tooth. Copyright © 2001 John Wiley & Sons, Ltd.     

Preservation of fungi in archaeological charcoal

During the analysis of wood charcoal remains from archaeological sites, it is common to find different microorganisms and different forms of degradation present in the plant tissue. However, one may encounter difficulties when attempting to identify these microorganisms and determine when their attack occurred. This paper focuses on preservation aspects related to the microorganisms in wood and demonstrates the structural changes that take place in different types of decayed wood after it was converted into charcoal. The study seeks to determine whether the microbial attack found in archaeological woods took place before the burning of the wood or after. Burning experiments were conducted using wood that had been decayed by various types of fungi including white-rot, brown-rot, and soft-rot. The laboratory burnt wood samples showed decay patterns that were comparable to those observed in archaeological charcoal samples, indicating that signs of fungal infestation and features of decay can be preserved after burning with micromorphological details of mycelium and cell wall attack evident. This indication may provide important information related to the gathering of deadwood as fuelwood. In addition, examples of decayed wood preserved in archaeological charcoal assemblages are described. Their relationship to the archaeological context and environmental conditions may suggest different interpretative models concerning wood management strategies applied by past societies.     

Migration and mobility at the Late Lapita site of Reber–Rakival (SAC), Watom Island using isotope and trace element analysis: a new insight into Lapita interaction in the Bismarck Archipelago

This paper uses strontium isotope (⁸⁷Sr/⁸⁶Sr), oxygen isotope (δ¹⁸O) and Ba/Sr trace element data in archaeological tooth enamel samples to investigate migration and mobility at the Late Lapita site of SAC, Watom Island in the Bismarck Archipelago. Previous archaeological models have identified Lapita mobility at a community level using obsidian distribution patterns and changes in ceramic design, whereas isotope and trace element data can potentially reconstruct prehistoric mobility on an individual level. Human and pig teeth were sampled from SAC and a selection of human teeth were included from the Late–Post Lapita site of Lifafaesing, Tanga Islands as a geographic/geological comparison.The results indicate that there is a large amount of isotopic variation in the Bismarck Archipelago which is useful for identifying non-local individuals and possibly determining their origins. One human individual and several pigs were suggested as coming from elsewhere in the region. Three potentially separate locations were identified for the non-local pigs. It is argued, using the data from SAC, that Late Lapita communities in the Bismarck Archipelago were more mobile than previously assumed. The potential for identifying individual migrants in a Lapita context are discussed in terms of assessing the more subtle aspects of Lapita society in the Southwest Pacific Islands.     

Sample-specific (universal) metric approaches for determining the sex of immature human skeletal remains using permanent tooth dimensions

In this paper, three approaches for developing sample-specific sex determination methods of immature skeletal remains based on permanent tooth dimensions are proposed and tested using a sample of identified skeletons. The sample comprises adult and subadult individuals selected from the Lisbon documented skeletal collection, housed at the National Museum of Natural History in Lisbon, Portugal. Faciolingual and mesiodistal diameters were the tooth dimensions utilized. In the first approach, sex-specific logistic regression formulae based on adult tooth dimensions are developed and then used to determine the sex of the subadult sample. The second and third approaches are based on the sectioning point procedure, which uses the overall mean of a measurement (tooth diameter) collected from the sample as the discriminant criteria for determining the sex of the individuals in that sample. While in the second approach the adult overall mean of each dimension is used as the discriminant criteria for determining the sex of the subadults, in the third approach it is the subadult overall mean of each dimension that comprises the discriminant criteria that is applied back to the subadults for determining sex. Results show that the canines are the teeth with the highest sexual dimorphism and methods of sex determination based on canine dimensions provide correct allocation accuracies between 58.8% and 100% depending on the diameter and the approach that is being used. Canine faciolingual dimensions provide the best overall results. Combinations of measurements from the same and different teeth do not increase significantly the accuracy of the methods and approaches. Some of the problems of subadult sex determination methods based on adult tooth dimensions result from differing levels of sexual dimorphism between the adult and subadult segment of the sample. Mortality or cultural bias may increase or decrease the sexual dimorphism of the subadults compared to the adults. Small subadult samples utilized in this study may also raise questions regarding the accuracy of the three different sample-specific approaches. However, high consistency of results using the canine and different approaches, suggests that adult and subadult canine dimensions can be reliable sex discriminators of immature skeletal remains in archaeological samples. The major advantage of the approaches presented here is that they can be used to derive sample-specific methods and, therefore, eliminate the problem of applying morphological or metric methods to individuals originating from a population that differs from the one that contributed to the development of the method.     

Strontium isotope analysis of human tooth enamel from Barsinia: a late antiquity site in Northern Jordan

The archaeological site of Barsinia represents a model of a mixed subsistence strategy in the late antiquity of Jordan. Contrary to historians' belief that the late antiquity economy was stagnated, archaeological evidence at the site of Barsinia points to wealth accumulation as mirrored by the local wine industry and trade. As the economic growth may enhance population dynamics, the study tests the population mobility at the site using strontium isotope ratios from the human tooth enamel. The study comprised 12 right upper third molars and 12 rodent teeth samples. The results confirm that all of the sampled individuals were local to the area (raised in the area) and whose diets were probably obtained from spatially restricted localities in the region.     

Refining the Quadratic Crown Height Method of age estimation: do elk teeth wear quadratically with age?

Teeth are the basis for the best methods for estimating the age-at-death of archaeological and paleontological faunal remains, because they change by eruption and wear throughout an individual's life and because they preserve well. However, age-at-death can be difficult to estimate when teeth are isolated or when no known-age reference sample is available. For these reasons, researchers developed the Quadratic Crown Height Method (QCHM), a set of quadratic formulae that can be used to predict age-at-death from tooth crown height, when unworn crown height and the ages when the tooth erupts and when its crown height should reach zero can be estimated. Previous tests of the QCHM suggest that modified equations could improve the method. Here, we use crown height measurements on a sample of 226 known-age Rocky Mountain elk (Cervus elaphus) to perform such modifications. We adjust the age at which each tooth type's crown height reaches zero from the species' potential ecological longevity or average maximum life span to an age that we empirically estimate for each tooth type. We also empirically assess whether for different elk teeth the exponent in the QCHM formula is actually equal to 2; it is for M1, but for P4 it is about 1, indicating a roughly linear relationship. The exponents for M2 and M3 are intermediate, being closer to 1.5. Because different teeth wear at different rates and wear completely away at different ages, we recommend that researchers use the modified equations provided here to estimate age-at-death for samples of Cervus elaphus.     

Cut and Tooth Mark Distributions on Large Animal Bones: Ethnoarchaeological Data from the Hadza and Their Implications For Current Ideas About Early Human Carnivory

Distributions of cut and tooth marks on the bones of large animals found in archaeological sites are increasingly used as sources of inference about the relative importance of hunting and scavenging in early human diets, and (by extension) about the role of meat-eating in human evolution. Here we review the empirical basis for these inferences in light of ethnoarchaeological data from the Tanzanian Hadza, a modern East African foraging population. Comparison of the Hadza data with those produced by other actualistic work indicates that while there may be a relationship between cut and tooth mark distributions and order of consumer access (human- versus carnivore-first), it is less clear-cut than many have suggested. Application of these results to the analysis of Plio-Pleistocene archaeological collections is further complicated by inconsistencies in the ways cut and tooth marks have been defined and counted, and by significant differences between patterns observed in modern control samples and those reported at ancient sites. These observations indicate that cut and tooth mark analyses are unlikely to speak effectively to questions about early human carnivory in the absence of: (1) better-warranted, more comprehensive expectations about the potential range of variation in past human carcass acquisition strategies, (2) a larger, more rigorously designed set of control experiments that model the archaeological consequences of these strategies, and (3) a larger, more consistently analysed archaeological data base. Even if these requirements are met, the idea of meat-eating as an important catalyst in the evolution of early humans will remain highly problematic, mainly due to problems involving the frequency and short-term reliability of carcass access.     

Activity-induced musculoskeletal stress markers (MSM) and subsistence strategy changes among ancient Hudson Bay Eskimos

Although archaeological evidence may express the results of several seasons of activity, the human skeleton, when correlated with archaeological and ethnographic data, provides information concerning daily activities performed throughout an individual's lifetime. Studies in occupational and sports medicine, along with electromyographic analysis of movement, have shown that different activities place different amounts of stress on human bone. In the present study, analysis of upper extremity musculoskeletal stress markers (MSM) has been used to clarify habitual activity patterns of two ancient Thule Eskimo groups from northwest Hudson Bay, Canada. Distinct pattern differences in muscle use occurred between Thule adult males and females and suggest possible gender-specific activity patterns that are not always discernible from the archaeological record alone. Temporal applications of the MSM data for Early and Late Period Thule support McCartney's theory of a substantial change in subsistence strategies through time, particularly among the adult males.     

Report of three fused primary human teeth in an archaeological material

Fusion of deciduous teeth can appear in several ways, usually involving two teeth but occasionally three. The frequency of this anomaly is reported to be low and few cases have been described in the scientific literature. Fused teeth in the archaeological record are rare because of the destructive taphonomic processes to which bones and teeth are subjected. Previous research in this field has only dealt with cases of two fused deciduous teeth. To our knowledge, there have been no reports of three fused teeth in the anthropological literature. The present paper presents a case of three fused primary teeth and a succedaneous supernumerary permanent tooth in a 5‐year‐old child discovered in a late medieval cemetery in northern Italy. Copyright © 2009 John Wiley & Sons, Ltd.     

Bone surface modifications in zooarchaeology

Cutmarks made by stone tools, conchoidal flake scars from hammerstone percussion, carnivore tooth marks, striations from sedimentary abrasion, and other surface modifications on bones from archaeological sites constitute a crucial body of evidence for investigating the role of human behaviors and of nonhuman taphonomic processes in site formation. This paper describes the various kinds of bone surface modifications produced by humans and by nonhuman processes and assesses the current status of bone surface modification studies with regard to such issues as the need for greater analytical standardization, the selection of instruments for examining bone specimens, tactics for identifying the origins of marks on bones, and strategies for inferring human behaviors.     

Sex estimation using cervical dental measurements in an archaeological population from Iran

Sex estimation of skeletal remains is one of the major components of forensic identification of unknown individuals. Teeth are a potential source of information on sex and are often recovered in archaeological or forensic contexts due to their post-mortem longevity. Currently, there is limited data on dental sexual dimorphism of archaeological populations from Iran. This paper represents the first study to provide a dental sex estimation method for Iron Age populations. The current study was conducted on the skeletal remains of 143 adults from two Iron Age populations in close temporal and geographic proximity in the Solduz Valley (West Azerbaijan Province of Iran). Mesiodistal and buccolingual cervical measurements of 1334 maxillary and mandibular teeth were used to investigate the degree of sexual dimorphism in permanent dentition and to assess their applicability in sex estimation. Data was analysed using discriminant function analysis (SPSS 23), and posterior probabilities were calculated for all produced formulae. The results showed that incisors and canines were the most sexually dimorphic teeth, providing percentages of correct sex classification between 86.4 and 100 % depending on the measurement used. The combination of canines and other teeth improved significantly the level of correct sex classification. The highest percentages of sex classification were obtained by the combination of canines and incisors (100 %) and canines and molars (92.3 %). The present study provided the first reference standards for sex estimation using odontometric data in an Iranian archaeological population. Cervical measurements were found to be of value for sex assessment, and the method presented here can be a useful tool for establishing accurate demographic data from skeletal remains of the Iron Age from Iran.     

Determining Age and Season of Death by Use of Incremental Lines in Norwegian Reindeer Tooth Cementum

Using incremental patterns in tooth cementum is a powerful tool for age assessment. Recent developments have shown that the method has a large potential as an indicator of the season of death of the individual in question. In this paper, the results of a study comparing thin sections of teeth of the modern Hardangervidda reindeer population to those of Iron Age, eleventh, and thirteenth century reindeer hunting stations from the same area are presented. The tooth wear stages were compared to the actual age of the individual (based on the number of incremental lines and the age at eruption). It was established that in the studied reindeer populations wear stages sometimes result in too low an age estimate. Having identified the start of the period of deposition for rest lines in the modern reindeer population as early autumn and winter, analysis of the outermost layer in the tooth cementum at the archaeological sites points to a prolonged hunting season in the thirteenth century, while in the earlier phases hunters were probably present on fewer and shorter visits.     

Lead isotope ratios: Tracking the migration of European‐Americans to Grafton,Illinois in the 19th century

Lead isotope analysis was conducted using inductively coupled plasma mass spectrometer (ICP‐MS) instruments on local soil samples and human premolar tooth enamel from a 19th century population from Grafton, Illinois, USA. The goal of the study was to determine if lead isotope analysis could be used to infer place of birth and patterns of 19th century migration into the city of Grafton. Five soil core samples from a location near Grafton, Illinois, five grave soil samples from the city cemetery and the tooth enamel of 19 human premolars were analysed. The results of the soil core analysis indicated that the lead isotopic signature of Grafton differs significantly from isotope ratios of other geographic areas associated with recorded places of birth of 19th century Jersey County residents. Elemental and isotope analysis of the soil samples indicated that diagenesis was not a factor in the analysis of lead isotopic signatures of enamel. From the lead isotope analysis of human premolars, the geographic origin of 13 of the remaining 15 individuals could be inferred. The inferred geographic origin was supplemented by an analysis of 1860 mortality and census records and demonstrated the utility of using lead isotope analysis in bio‐archaeological investigations. Copyright © 2010 John Wiley & Sons, Ltd.     

Exponentially decreasing tooth growth rate in horse teeth: implications for isotopic analyses

A range of archaeological and palaeoclimatic studies use isotopic analyses of ungulate hypsodont tooth enamel. Such studies commonly assume a constant growth rate, though this has not been fully tested. Here, we use stable isotope analyses of sequential enamel samples to study horse tooth growth. We fit the data using models corresponding to constant and exponentially decreasing rates of growth, and compare the results to metrical data showing the geometry and timing of apposition. The results indicate enamel apposition and maturation advances at an exponentially decreasing rate. An understanding of this variable growth rate is crucial for interpreting isotopic data from equid teeth.     

Fruits of Their Labour: Urbanisation,Orchard Crops,and Dental Health in Early Bronze Age Jordan

The analysis of dental remains, which outlast most other tissues in the human body, provides insight into past diet, activity patterns and ancestry. The remains from Bab edh‐Dhra' represent the only skeletal sample available to assess the impact of agricultural intensification in the Early Bronze Age of the southern Levant (ca. 3500–2000 bce ). This era ushered in a period of ‘urbanisation’, evidenced by fortified towns, planned roadways, developments in irrigation and growing population density. During this time, the cultivation, trade and consumption of orchard taxa (such as figs, grapes and olives) increased. This paper examines changes in the teeth associated with agricultural intensification involving orchard crops as well as grains. Dental caries, ante mortem tooth loss and dental wear are examined for Early Bronze IA (EBIA; 3500–3300 bce ) and Early Bronze II–III (EBII–III; 3100–2300 bce ) teeth from the site of Bab edh‐Dhra', located in modern‐day Jordan. Due to the commingling, general tooth groups (e.g. molars) and specific tooth types (e.g. lower left canine) were used to compare periods. Although age and sex could not be identified for every tooth, analyses of crania and os coxae showed no significant difference in demographic profiles of EBIA and EBII–III. No statistically significant increase was found over time in dental caries frequency; however, teeth for which the cause of pulp exposure could be determined suggested that caries increasingly led to exfoliation. Indeed, ante mortem tooth loss rose significantly with time, whereas dental wear decreased. In general, changes in oral health were consistent with an archaeological record of greater consumption of softer, stickier foods, such as fruits. Copyright © 2013 John Wiley & Sons, Ltd.     

The spatial distribution of Palaeolithic human settlements and its influence on palaeoecological studies: a case from Northern Iberia

The aim of this study is to assess the influence of human site location choice on biogeographical and paleoecological studies based on archaeological remains, through a case study in north-western Spain. Data from an exhaustive literature survey and field trips were managed with GIS and subjected to statistical analyses. The results show that the influence of the different variables shifted through the Palaeolithic, although certain general preferences can be seen: low altitudes (median: 151 m a.s.l.), South-facing surfaces, generally gentle slopes (median: 13°) and moderate distances to water courses (median: 297 m). These choices were conditioned by geomorphological factors, with LGM glacier extent imposing an upper limit in the range of occupied altitudes and marine and fluvial terraces conditioning site location patterns. The results suggest that human site location patterns during the Palaeolithic were not random, conditioning the information available from archaeological remains. Our results allow us to identify some key areas where information on past faunal distributions, and more generally on biocoenoses, will be scarce or missing.     

Peck-Marked Vessels from the San José Market Street Chinatown: A Study of Distribution and Significance

Ceramic bowls and plates with Chinese characters pecked into their surfaces are documented on almost every nineteenth- and early twentieth-century Chinatown site in California. Typically, these vessels are said to bear marks of ownership, and further analysis has been uncommon. Given the socio-political atmosphere surrounding Chinese immigration and labor during this time period, as well as the cultural relevance of this marking practice, it is the author's belief that this explanation is incomplete. Through analysis of archaeological materials from the Market Street Chinatown in San José, California, this paper explores the possibility that Chinese immigrants were using and hybridizing the familiar Chinese cultural practice of marking vessels to aid in creating an environment within the Chinatown that was both more comfortable and more livable.     

Diagnostic criteria for identification of seashell as a trephination implement

Scanning electron microscopy has been applied to experimentally cut bone samples and an ethnographical example of trephination on a human skull in order to produce diagnostic criteria for the identification of mollusc shells as cutting implements used in trephination. Shell scrapes are identifiable by parallel striations in the direction of cut, crossed at 90° by chatter marks caused by uneven movement of the fragile shell-edge across the bone surface.